1TV6
HIV-1 Reverse Transcriptase Complexed with CP-94,707
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-10-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 223.910, 69.040, 104.330 |
| Unit cell angles | 90.00, 106.64, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.262 |
| Rwork | 0.262 |
| R-free | 0.31200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3hvt |
| RMSD bond length | 0.096 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.058 | |
| Number of reflections | 37716 | |
| <I/σ(I)> | 29.1 | 1.6 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 50 mM bis-Tris-propane, 100 mM ammonium sulfate, 0.2% (w/v) beta-octylglucoside, 10% (v/v) glycerol, 14% (w/v) PEG-8000, pH 7.0 |
