1NVU

Structural evidence for feedback activation by RasGTP of the Ras-specific nucleotide exchange factor SOS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-03
Detector	SBC-2
Wavelength(s)	1.0332
Spacegroup name	I 4 2 2
Unit cell lengths	183.312, 183.312, 178.509
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.000 * - 2.210 *
R-factor	0.186
Rwork	0.186
R-free	0.21200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bkd
RMSD bond length	0.009
RMSD bond angle	21.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.110 *	0.512 *
Total number of observations	758703 *
Number of reflections	75882
<I/σ(I)>	18.7	3.3
Completeness [%]	99.4	99.9
Redundancy	10	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	1.2-1.5 M phosphate, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	reservoir	phosphate	1.2-1.5 (M)
3	1	reservoir	HEPES	100 (mM)	pH7.5