1K3A

Structure of the Insulin-like Growth Factor 1 Receptor Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97250
Spacegroup name	C 2 2 21
Unit cell lengths	80.624, 111.018, 93.297
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 * - 2.100
R-factor	0.212
Rwork	0.212
R-free	0.24800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ir3
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000 *	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.048 *	0.254 *
Total number of observations	279673 *
Number of reflections	24941
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	4 *	PEG 8000, sodium chloride, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Mg AMP-PCP	1 (mM)
3	1	drop	peptide	1 (mM)
4	1	reservoir	PEG8000	16 (%(w/v))
5	1	reservoir	HEPES	100 (mM)	pH7.5
6	1	reservoir		0.15 (M)
7	1	reservoir	ethylene glycol	2 (%(v/v))