1JTY
Crystal structure of the multidrug binding transcriptional regulator QacR bound to ethidium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 172.000, 172.000, 95.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.160 - 2.970 |
| Rwork | 0.239 |
| R-free | 0.29100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1jus |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.160 | 3.150 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Rmerge | 0.045 | 0.229 |
| Number of reflections | 22060 | |
| <I/σ(I)> | 12 | 3 |
| Completeness [%] | 75.0 | 67.6 |
| Redundancy | 5.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Ammonium sulphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
