1F93

CRYSTAL STRUCTURE OF A COMPLEX BETWEEN THE DIMERIZATION DOMAIN OF HNF-1 ALPHA AND THE COACTIVATOR DCOH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	CCD
Collection date	1998-01-01
Detector	ADSC QUANTUM
Spacegroup name	P 1 21 1
Unit cell lengths	49.480, 82.750, 70.640
Unit cell angles	90.00, 97.83, 90.00

Refinement procedure

Resolution	20.100 - 2.600
R-factor	0.257
Rwork	0.257
R-free	0.29900
RMSD bond length	0.006
RMSD bond angle	1.103 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.670
High resolution limit [Å]	2.600	2.600
Rmerge	0.057 *	0.243 *
Total number of observations	59400 *
Number of reflections	16500
<I/σ(I)>	22
Completeness [%]	95.0	95
Redundancy	3.6	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5	4 *	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	PEG8000	13 (%(w/v))
3	1	drop	potassium succinate	100 (mM)
4	1	reservoir	PEG400	15 (%(v/v))
5	1	reservoir	PEG8000	14 (%(w/v))
6	1	reservoir	potassium succinate	100 (mM)