1C8T

HUMAN STROMELYSIN-1 (E202Q) CATALYTIC DOMAIN COMPLEXED WITH RO-26-2812

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	1995-01-01
Detector	SDMS
Spacegroup name	P 21 21 21
Unit cell lengths	120.100, 47.000, 54.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.22
RMSD bond length	0.011 *
RMSD bond angle	2.500 *
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.150	2.150
Rmerge	0.079
Number of reflections	15629
<I/σ(I)>	19
Completeness [%]	88.9 *
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	22 *	PEG, potassium chloride, calcium chloride, cacodylate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	mPEG6000	100 (mM)	or mPEG8000
2	1	reservoir		50 (mM)
3	1	reservoir		200 (mM)