1A4R
G12V MUTANT OF HUMAN PLACENTAL CDC42 GTPASE IN THE GDP FORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 98.650, 98.650, 104.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.225 * |
| Rwork | 0.228 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4Q21 WITH ALL NON-CONSERVED RESIDUES MUTATED TO ALANINE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 27.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.049 | 0.460 |
| Total number of observations | 88071 * | |
| Number of reflections | 18254 * | |
| <I/σ(I)> | 15.6 | 3.3 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * | 15 * | pH 4.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mM) | |
| 2 | 1 | drop | HEPES-NaOH | 20 (mM) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | reservoir | 0.1 (M) | ||
| 5 | 1 | reservoir | PEG4000 | 5 (%) | |
| 6 | 1 | reservoir | 0.3 (M) |
