+Open data
-Basic information
Entry | Database: PDB / ID: 7te4 | |||||||||
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Title | Crystal structure of Fab2 anti-GluN2B antibody | |||||||||
Components |
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Keywords | IMMUNE SYSTEM / Fab fragment | |||||||||
Biological species | Mus musculus (house mouse) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.456 Å | |||||||||
Authors | Tajima, N. / Furukawa, H. | |||||||||
Funding support | United States, 2items
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Citation | Journal: Nat Commun / Year: 2022 Title: Development and characterization of functional antibodies targeting NMDA receptors. Authors: Nami Tajima / Noriko Simorowski / Remy A Yovanno / Michael C Regan / Kevin Michalski / Ricardo Gómez / Albert Y Lau / Hiro Furukawa / Abstract: N-methyl-D-aspartate receptors (NMDARs) are critically involved in basic brain functions and neurodegeneration as well as tumor invasiveness. Targeting specific subtypes of NMDARs with distinct ...N-methyl-D-aspartate receptors (NMDARs) are critically involved in basic brain functions and neurodegeneration as well as tumor invasiveness. Targeting specific subtypes of NMDARs with distinct activities has been considered an effective therapeutic strategy for neurological disorders and diseases. However, complete elimination of off-target effects of small chemical compounds has been challenging and thus, there is a need to explore alternative strategies for targeting NMDAR subtypes. Here we report identification of a functional antibody that specifically targets the GluN1-GluN2B NMDAR subtype and allosterically down-regulates ion channel activity as assessed by electrophysiology. Through biochemical analysis, x-ray crystallography, single-particle electron cryomicroscopy, and molecular dynamics simulations, we show that this inhibitory antibody recognizes the amino terminal domain of the GluN2B subunit and increases the population of the non-active conformational state. The current study demonstrates that antibodies may serve as specific reagents to regulate NMDAR functions for basic research and therapeutic objectives. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7te4.cif.gz | 163.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7te4.ent.gz | 124.3 KB | Display | PDB format |
PDBx/mmJSON format | 7te4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7te4_validation.pdf.gz | 451.5 KB | Display | wwPDB validaton report |
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Full document | 7te4_full_validation.pdf.gz | 459.7 KB | Display | |
Data in XML | 7te4_validation.xml.gz | 28 KB | Display | |
Data in CIF | 7te4_validation.cif.gz | 38.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/te/7te4 ftp://data.pdbj.org/pub/pdb/validation_reports/te/7te4 | HTTPS FTP |
-Related structure data
Related structure data | 7te6C 7te9C 7tebC 7teeC 7teqC 7terC 7tesC 7tetC 5b3jS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
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