[English] 日本語
Yorodumi- PDB-2hwz: Fab fragment of Humanized anti-viral antibody MEDI-493 (Synagis TM) -
+Open data
-Basic information
Entry | Database: PDB / ID: 2hwz | ||||||
---|---|---|---|---|---|---|---|
Title | Fab fragment of Humanized anti-viral antibody MEDI-493 (Synagis TM) | ||||||
Components |
| ||||||
Keywords | IMMUNE SYSTEM / Immunoglobulin / Fab fragment | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta Function and homology information | ||||||
Biological species | Homo sapiens (human) Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.8 Å | ||||||
Authors | Braden, B. | ||||||
Citation | Journal: To be Published Title: Identification of a single tryptophan residue as critical for binding activity in a humanized monoclonal antibody against respiratory syncytial virus Authors: Wei, Z. / Feng, J. / Lin, H.-Y. / Mullapudi, S. / Bishop, E. / Tous, G. / Casas-Finet, J. / Hakki, F. / Strouse, R. / Schenerman, M. #1: Journal: Patent / Year: 2005 Title: Crystals and structure of Synagis Fab Authors: Braden, B. / Johnson, L.S. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2hwz.cif.gz | 99 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2hwz.ent.gz | 79.2 KB | Display | PDB format |
PDBx/mmJSON format | 2hwz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2hwz_validation.pdf.gz | 370 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 2hwz_full_validation.pdf.gz | 377.1 KB | Display | |
Data in XML | 2hwz_validation.xml.gz | 10.2 KB | Display | |
Data in CIF | 2hwz_validation.cif.gz | 17.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hw/2hwz ftp://data.pdbj.org/pub/pdb/validation_reports/hw/2hwz | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Details | Fab heterodimer of antibody light chain and heavy chain variable and constant domain 1 |
-Components
#1: Antibody | Mass: 23177.904 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Humanized mouse antibody MEDI-493 Anti-respiratory syncytial virus antibody Source: (gene. exp.) Homo sapiens, Mus musculus |
---|---|
#2: Antibody | Mass: 24246.334 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Humanized mouse antibody MEDI-493 Anti-respiratory syncytial virus antibody Source: (gene. exp.) Homo sapiens, Mus musculus |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.99 Å3/Da / Density % sol: 58.82 % |
---|
-Data collection
Diffraction source | Source: ROTATING ANODE / Type: RIGAKU |
---|---|
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 1.8→6 Å / Num. obs: 33200 / Observed criterion σ(F): 2 |
-Processing
Software |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.8→6 Å / σ(F): 2 / Stereochemistry target values: Engh & Huber /
| ||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→6 Å
| ||||||||||||
LS refinement shell | Resolution: 1.8→1.9 Å /
|