mitochondrial genome maintenance / ribonuclease III activity / fungal-type vacuole / DNA strand exchange activity / mitochondrial large ribosomal subunit / mitochondrial translation / RNA processing / large ribosomal subunit / double-stranded RNA binding / single-stranded DNA binding ...mitochondrial genome maintenance / ribonuclease III activity / fungal-type vacuole / DNA strand exchange activity / mitochondrial large ribosomal subunit / mitochondrial translation / RNA processing / large ribosomal subunit / double-stranded RNA binding / single-stranded DNA binding / transferase activity / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / rRNA binding / negative regulation of translation / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / mitochondrion / RNA binding 類似検索 - 分子機能
54S ribosomal protein L44, mitochondrial / : / Mitochondrial ribosomal protein subunit L20 / Ribosomal protein L31p, N-terminal / Ribosomal protein L20, mitochondrial / Mitochondrial homologous recombination protein 1 / 54S ribosomal protein L25 / 54S ribosomal protein L15, mitochondrial / MRPL25 domain / 54S ribosomal protein L3, double-stranded RNA binding domain ...54S ribosomal protein L44, mitochondrial / : / Mitochondrial ribosomal protein subunit L20 / Ribosomal protein L31p, N-terminal / Ribosomal protein L20, mitochondrial / Mitochondrial homologous recombination protein 1 / 54S ribosomal protein L25 / 54S ribosomal protein L15, mitochondrial / MRPL25 domain / 54S ribosomal protein L3, double-stranded RNA binding domain / Transcriptional regulation of mitochondrial recombination / Mitochondrial ribosomal protein mL59 / 54S ribosomal protein L28, mitochondrial / Ribosomal protein L31, mitochondrial / 54S ribosomal protein L36, yeast / Mitochondrial ribosomal protein L31 / Ribonuclease-III-like / 28S ribosomal protein S25, mitochondrial / Ribonuclease III family domain profile. / Ribonuclease III family / Ribonuclease III domain / Ribosomal protein L37, mitochondrial / Mitochondrial ribosomal protein L37 / Ribosomal protein L53, mitochondrial / 39S ribosomal protein L53/MRP-L53 / Ribosomal protein L49/IMG2 / Mitochondrial large subunit ribosomal protein (Img2) / Ribosomal protein L46, N-terminal / 39S mitochondrial ribosomal protein L46 / Ribosomal protein L50, mitochondria / Ribosomal protein L27/L41, mitochondrial / Mitochondrial ribosomal protein L27 / Ribosomal subunit 39S / 39S ribosomal protein L46, mitochondrial / 39S ribosomal protein L43/54S ribosomal protein L51 / Ribosomal protein L47, mitochondrial / MRP-L47 superfamily, mitochondrial / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein, eukaryotic / PEBP-like superfamily / Double-stranded RNA binding motif / Double-stranded RNA binding motif / Ribonuclease III, endonuclease domain superfamily / Double-stranded RNA-binding domain / : / NUDIX domain / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / NUDIX hydrolase domain / Ribosomal protein L1, 3-layer alpha/beta-sandwich / NUDIX hydrolase-like domain superfamily / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L1p/L10e family / Ribosomal protein L11, bacterial-type / Ribosomal protein L10-like domain superfamily / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal protein L11, N-terminal / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11, RNA binding domain / Ribosomal protein L11/L12 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L6, bacterial-type / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L14P, bacterial-type / Ribosomal protein L2, bacterial/organellar-type / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / Ribosomal protein L30, bacterial-type / : / Ribosomal protein L16 / L28p-like / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal protein L19 / Ribosomal protein L17 類似検索 - ドメイン・相同性
: / NICOTINAMIDE-ADENINE-DINUCLEOTIDE / SPERMINE / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Ribosomal protein L15 / Large ribosomal subunit protein mL59 domain-containing protein / Uncharacterized protein ...: / NICOTINAMIDE-ADENINE-DINUCLEOTIDE / SPERMINE / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Ribosomal protein L15 / Large ribosomal subunit protein mL59 domain-containing protein / Uncharacterized protein / Large ribosomal subunit protein uL16m / Large ribosomal subunit protein bL32m / Large ribosomal subunit protein uL1m / Large ribosomal subunit protein uL5m / Large ribosomal subunit protein mL58 / Large ribosomal subunit protein bL27m / Large ribosomal subunit protein bL31m / Large ribosomal subunit protein bL17m / Large ribosomal subunit protein uL3m / Large ribosomal subunit protein uL30m / Large ribosomal subunit protein mL61 / Large ribosomal subunit protein uL11m / Large ribosomal subunit protein uL24m / Large ribosomal subunit protein mL38 / Large ribosomal subunit protein mL67 / Large ribosomal subunit protein bL19m / Large ribosomal subunit protein uL10m / Large ribosomal subunit protein uL6m / Large ribosomal subunit protein bL9m / Large ribosomal subunit protein mL57 / Large ribosomal subunit protein mL46 / Large ribosomal subunit protein mL43 / Large ribosomal subunit protein bL36m / Large ribosomal subunit protein mL49 / Large ribosomal subunit protein uL22m / Large ribosomal subunit protein mL41 / Large ribosomal subunit protein mL50 / Large ribosomal subunit protein uL29m / Large ribosomal subunit protein uL23m / Large ribosomal subunit protein mL44 / Large ribosomal subunit protein uL14m / Large ribosomal subunit protein uL13m / Large ribosomal subunit protein bL28m / Large ribosomal subunit protein uL2m / Large ribosomal subunit protein mL54 / Large ribosomal subunit protein bL21m / Large ribosomal subunit protein mL53 / Related to ribosomal protein L34, mitochondrial / Large ribosomal subunit protein mL60 / Large ribosomal subunit protein bL33m / Large ribosomal subunit protein uL4m 類似検索 - 構成要素
ジャーナル: Nat Commun / 年: 2020 タイトル: Analysis of translating mitoribosome reveals functional characteristics of translation in mitochondria of fungi. 著者: Yuzuru Itoh / Andreas Naschberger / Narges Mortezaei / Johannes M Herrmann / Alexey Amunts / 要旨: Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each ...Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each other is not understood. We report models of the translating fungal mitoribosome with mRNA, tRNA and nascent polypeptide, as well as an assembly intermediate. Nicotinamide adenine dinucleotide (NAD) is found in the central protuberance of the large subunit, and the ATPase inhibitory factor 1 (IF) in the small subunit. The models of the active mitoribosome explain how mRNA binds through a dedicated protein platform on the small subunit, tRNA is translocated with the help of the protein mL108, bridging it with L1 stalk on the large subunit, and nascent polypeptide paths through a newly shaped exit tunnel involving a series of structural rearrangements. An assembly intermediate is modeled with the maturation factor Atp25, providing insight into the biogenesis of the mitoribosomal large subunit and translation regulation.
A: RNA (2820-MER) B: 60S ribosomal protein L2 C: 60S ribosomal protein L3 D: 60S ribosomal protein L4, variant E: 50S ribosomal protein L5 F: 60S ribosomal protein L6 G: RIBOSOMAL_L9 domain-containing protein f: Uncharacterized protein g: 60S ribosomal protein L19 H: Ribosomal protein L13 I: 50S ribosomal protein L14 J: Ribosomal protein L15 K: 60S ribosomal protein L16 L: 50S ribosomal protein L17 M: Mitochondrial ribosomal protein N: Aconitate hydratase O: Mitochondrial large ribosomal subunit P: Mitochondrial ribosomal protein subunit L23 Q: KOW domain-containing protein R: Uncharacterized protein S: 50S ribosomal protein L24 T: 54S ribosomal protein L4, mitochondrial U: 50S ribosomal protein L30 V: Uncharacterized protein W: Mitochondrial ribosomal protein subunit L32 X: Uncharacterized protein Y: Related to ribosomal protein L34, mitochondrial 0: Ribosomal protein 1: Mitochondrial large ribosomal subunit YmL35 2: Uncharacterized protein 3: Uncharacterized protein 4: Mitochondrial ribosomal protein L43 5: 60S ribosomal protein L3 6: 50S ribosomal subunit L30 7: Uncharacterized protein 8: Uncharacterized protein h: Mitochondrial ribosomal protein L44 i: Uncharacterized protein 9: RNase III domain-containing protein a: 60S ribosomal protein L20 b: Mitoc_mL59 domain-containing protein c: 54S ribosomal protein L31, mitochondrial d: Uncharacterized protein e: 60S ribosomal protein L1 j: L51_S25_CI-B8 domain-containing protein ヘテロ分子