3-hydroxyisobutyryl-CoA hydrolase / 3-hydroxyisobutyryl-CoA hydrolase activity / mitochondrial translational initiation / L-valine catabolic process / ATPase inhibitor activity / mitochondrial large ribosomal subunit / mitochondrial small ribosomal subunit / mitochondrial translation / superoxide dismutase activity / positive regulation of proteolysis ...3-hydroxyisobutyryl-CoA hydrolase / 3-hydroxyisobutyryl-CoA hydrolase activity / mitochondrial translational initiation / L-valine catabolic process / ATPase inhibitor activity / mitochondrial large ribosomal subunit / mitochondrial small ribosomal subunit / mitochondrial translation / superoxide dismutase activity / positive regulation of proteolysis / peroxisome / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / mitochondrion / RNA binding / metal ion binding / cytoplasm 類似検索 - 分子機能
54S ribosomal protein L36, yeast / : / : / : / Ribosomal protein L31p, N-terminal / Mitochondrial ribosomal protein MRP51, fungi / Mitochondrial ribosomal protein S25 / Ribosomal protein S24, mitochondrial / Ribosomal protein S23, mitochondrial, fungi / Mitochondrial ribosomal protein subunit ...54S ribosomal protein L36, yeast / : / : / : / Ribosomal protein L31p, N-terminal / Mitochondrial ribosomal protein MRP51, fungi / Mitochondrial ribosomal protein S25 / Ribosomal protein S24, mitochondrial / Ribosomal protein S23, mitochondrial, fungi / Mitochondrial ribosomal protein subunit / Mitochondrial ribosomal protein S25 / Ribosomal protein S35, mitochondrial / Ribosomal protein MRP10, mitochondrial / Eukaryotic mitochondrial regulator protein / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type / Small ribosomal subunit protein mS41 SAM domain / Enoyl-CoA hydratase/isomerase domain / Enoyl-CoA hydratase/isomerase / Protein Fyv4 / IGR protein motif / IGR / Mitochondrial ATPase inhibitor / Mitochondrial ATPase inhibitor, IATP / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Enoyl-CoA hydratase/isomerase, conserved site / Enoyl-CoA hydratase/isomerase signature. / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / Manganese/iron superoxide dismutase, C-terminal / Manganese/iron superoxide dismutase, C-terminal domain superfamily / Manganese/iron superoxide dismutase, N-terminal domain superfamily / Iron/manganese superoxide dismutases, C-terminal domain / ClpP/crotonase-like domain superfamily / Sterile alpha motif/pointed domain superfamily / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein S21 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / Ribosomal protein S15, bacterial-type / Ribosomal protein S12, bacterial-type / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S2 signature 1. / Ribosomal protein S15/S19, conserved site / Ribosomal protein S19 signature. / Ribosomal protein S10 / Ribosomal protein S19/S15 / Ribosomal protein S19/S15, superfamily / Ribosomal protein S19 / Ribosomal protein S2, conserved site / Ribosomal protein S2 / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S2 / Ribosomal protein S5 / Ribosomal protein S13, conserved site / Ribosomal protein S13 signature. / S5 double stranded RNA-binding domain profile. / Ribosomal protein S5, N-terminal / Ribosomal protein S13 / 30s ribosomal protein S13, C-terminal / Ribosomal protein S13/S18 / Ribosomal protein S5, C-terminal / Ribosomal protein S13 family profile. / Ribosomal protein S5, N-terminal domain / Ribosomal protein S5, C-terminal domain / Ribosomal protein S4, conserved site / Ribosomal protein S4 signature. / Ribosomal protein S14 / Ribosomal protein S14p/S29e / Ribosomal protein S4/S9 / Ribosomal protein S8 / Ribosomal protein S8 superfamily / Ribosomal protein S8 / S4 RNA-binding domain profile. / Ribosomal protein S13-like, H2TH / S4 RNA-binding domain / S4 domain / RNA-binding S4 domain / Ribosomal protein S10p/S20e / Ribosomal protein S9, conserved site / Ribosomal protein S9 signature. / Ribosomal protein S11 / Ribosomal protein S10 domain / Ribosomal protein S10 domain superfamily 類似検索 - ドメイン・相同性
Small ribosomal subunit protein bS1m / Small ribosomal subunit protein bS16m / Small ribosomal subunit protein uS3m / Small ribosomal subunit protein uS5m / Small ribosomal subunit protein uS15m / Small ribosomal subunit protein mS33 / Small ribosomal subunit protein mS35 / Small ribosomal subunit protein mS41 / Small ribosomal subunit protein mS47 / Large ribosomal subunit protein bL31m ...Small ribosomal subunit protein bS1m / Small ribosomal subunit protein bS16m / Small ribosomal subunit protein uS3m / Small ribosomal subunit protein uS5m / Small ribosomal subunit protein uS15m / Small ribosomal subunit protein mS33 / Small ribosomal subunit protein mS35 / Small ribosomal subunit protein mS41 / Small ribosomal subunit protein mS47 / Large ribosomal subunit protein bL31m / Small ribosomal subunit protein bS18m / Small ribosomal subunit protein uS19m / Small ribosomal subunit protein uS10m / Small ribosomal subunit protein mS27 / Small ribosomal subunit protein uS13m / Small ribosomal subunit protein mS42 / Small ribosomal subunit protein uS17m / Small ribosomal subunit protein mS37 / Small ribosomal subunit protein uS7m / Small ribosomal subunit protein uS9m / Small ribosomal subunit protein uS12m / Small ribosomal subunit protein uS4m / Small ribosomal subunit protein bS21m / Small ribosomal subunit protein bS6m / Small ribosomal subunit protein mS29 / Small ribosomal subunit protein uS14m / Small ribosomal subunit protein mS46 / Small ribosomal subunit protein uS11m / Small ribosomal subunit protein mS45 / Small ribosomal subunit protein uS8m / Small ribosomal subunit protein mS38 / Small ribosomal subunit protein mS26 / Small ribosomal subunit protein mS23 / Small ribosomal subunit protein uS2m / F(1)-ATPase inhibitor IF(1), mitochondrial 類似検索 - 構成要素
ジャーナル: Nat Commun / 年: 2020 タイトル: Analysis of translating mitoribosome reveals functional characteristics of translation in mitochondria of fungi. 著者: Yuzuru Itoh / Andreas Naschberger / Narges Mortezaei / Johannes M Herrmann / Alexey Amunts / 要旨: Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each ...Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each other is not understood. We report models of the translating fungal mitoribosome with mRNA, tRNA and nascent polypeptide, as well as an assembly intermediate. Nicotinamide adenine dinucleotide (NAD) is found in the central protuberance of the large subunit, and the ATPase inhibitory factor 1 (IF) in the small subunit. The models of the active mitoribosome explain how mRNA binds through a dedicated protein platform on the small subunit, tRNA is translocated with the help of the protein mL108, bridging it with L1 stalk on the large subunit, and nascent polypeptide paths through a newly shaped exit tunnel involving a series of structural rearrangements. An assembly intermediate is modeled with the maturation factor Atp25, providing insight into the biogenesis of the mitoribosomal large subunit and translation regulation.