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- EMDB-10977: The structure of the Atp25 bound assembly intermediate of the mit... -

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Basic information

Entry
Database: EMDB / ID: EMD-10977
TitleThe structure of the Atp25 bound assembly intermediate of the mitoribosome from Neurospora crassa
Map dataConsensus map for the assembly intermediate with bound Atp25
Sample
  • Complex: Assembly intermediate of the mitoribosome of Neurospora crassa
    • RNA: x 1 types
    • Protein or peptide: x 42 types
  • Ligand: x 5 types
Function / homology
Function and homology information


mitochondrial gene expression / DNA strand exchange activity / mitochondrial genome maintenance / fungal-type vacuole / ribonuclease III activity / mRNA stabilization / mitochondrial proton-transporting ATP synthase complex assembly / mitochondrial large ribosomal subunit / mitochondrial translation / RNA processing ...mitochondrial gene expression / DNA strand exchange activity / mitochondrial genome maintenance / fungal-type vacuole / ribonuclease III activity / mRNA stabilization / mitochondrial proton-transporting ATP synthase complex assembly / mitochondrial large ribosomal subunit / mitochondrial translation / RNA processing / large ribosomal subunit rRNA binding / double-stranded RNA binding / single-stranded DNA binding / large ribosomal subunit / cytosolic large ribosomal subunit / transferase activity / mitochondrial inner membrane / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / mitochondrion / RNA binding
Similarity search - Function
ATPase synthesis protein 25, mitochondrial / 54S ribosomal protein L44, mitochondrial / : / Mitochondrial ribosomal protein subunit L20 / Ribosomal protein L31p, N-terminal / Ribosomal protein L20, mitochondrial / Mitochondrial homologous recombination protein 1 / 54S ribosomal protein L25 / 54S ribosomal protein L15, mitochondrial / MRPL25 domain ...ATPase synthesis protein 25, mitochondrial / 54S ribosomal protein L44, mitochondrial / : / Mitochondrial ribosomal protein subunit L20 / Ribosomal protein L31p, N-terminal / Ribosomal protein L20, mitochondrial / Mitochondrial homologous recombination protein 1 / 54S ribosomal protein L25 / 54S ribosomal protein L15, mitochondrial / MRPL25 domain / 54S ribosomal protein L3, double-stranded RNA binding domain / Transcriptional regulation of mitochondrial recombination / Mitochondrial ribosomal protein mL59 / 54S ribosomal protein L28, mitochondrial / Ribosomal protein L31, mitochondrial / 54S ribosomal protein L36, yeast / Mitochondrial ribosomal protein L31 / Ribonuclease-III-like / Ribonuclease III family domain profile. / Ribosomal protein L37, mitochondrial / Mitochondrial ribosomal protein L37 / Ribonuclease III family / Ribonuclease III domain / Ribosomal protein L53, mitochondrial / 39S ribosomal protein L53/MRP-L53 / Ribosomal protein L49/IMG2 / Mitochondrial large subunit ribosomal protein (Img2) / Ribosomal protein L46, N-terminal / 39S mitochondrial ribosomal protein L46 / Ribosomal protein L50, mitochondria / Ribosomal protein L27/L41, mitochondrial / Mitochondrial ribosomal protein L27 / Ribosomal subunit 39S / 39S ribosomal protein L46, mitochondrial / Ribosomal protein L47, mitochondrial / MRP-L47 superfamily, mitochondrial / 39S ribosomal protein L43/54S ribosomal protein L51 / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein, eukaryotic / PEBP-like superfamily / Double-stranded RNA binding motif / Double-stranded RNA binding motif / Ribonuclease III, endonuclease domain superfamily / Double-stranded RNA-binding domain / : / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / NUDIX domain / NUDIX hydrolase domain / NUDIX hydrolase-like domain superfamily / Ribosomal protein L11, bacterial-type / Ribosomal protein L10-like domain superfamily / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L11, N-terminal / Ribosomal protein L17 signature. / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11, RNA binding domain / Ribosomal protein L28/L24 superfamily / Ribosomal protein L6, bacterial-type / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L14P, bacterial-type / Ribosomal protein L2, bacterial/organellar-type / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / L28p-like / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal protein L19 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal L32p protein family / Ribosomal protein L24 / Ribosomal protein L32p / Ribosomal protein L34
Similarity search - Domain/homology
Uncharacterized protein / Large ribosomal subunit protein uL16m / Large ribosomal subunit protein bL32m / Large ribosomal subunit protein uL5m / Large ribosomal subunit protein mL58 / Large ribosomal subunit protein bL27m / Large ribosomal subunit protein bL31m / Ribosomal protein L34 / Large ribosomal subunit protein bL17m / Large ribosomal subunit protein uL3m ...Uncharacterized protein / Large ribosomal subunit protein uL16m / Large ribosomal subunit protein bL32m / Large ribosomal subunit protein uL5m / Large ribosomal subunit protein mL58 / Large ribosomal subunit protein bL27m / Large ribosomal subunit protein bL31m / Ribosomal protein L34 / Large ribosomal subunit protein bL17m / Large ribosomal subunit protein uL3m / Large ribosomal subunit protein uL30m / Large ribosomal subunit protein uL11m / Large ribosomal subunit protein uL24m / Large ribosomal subunit protein mL38 / Large ribosomal subunit protein mL67 / Large ribosomal subunit protein bL19m / Large ribosomal subunit protein uL10m / Large ribosomal subunit protein uL6m / Large ribosomal subunit protein mL57 / Large ribosomal subunit protein mL46 / Large ribosomal subunit protein mL43 / Large ribosomal subunit protein bL36m / Large ribosomal subunit protein mL49 / Large ribosomal subunit protein uL22m / Large ribosomal subunit protein mL41 / Large ribosomal subunit protein mL50 / Large ribosomal subunit protein uL29m / Large ribosomal subunit protein uL23m / Large ribosomal subunit protein mL44 / ATPase synthesis protein 25, mitochondrial / 50S ribosomal subunit protein L15 / Large ribosomal subunit protein uL14m / Large ribosomal subunit protein uL13m / Large ribosomal subunit protein bL28m / Large ribosomal subunit protein uL2m / Large ribosomal subunit protein mL54 / Mitochondrial 60S ribosomal protein L25 / Large ribosomal subunit protein bL21m / Large ribosomal subunit protein mL53 / Large ribosomal subunit protein mL60 / Large ribosomal subunit protein bL33m / Large ribosomal subunit protein uL4m
Similarity search - Component
Biological speciesNeurospora crassa OR74A (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.03 Å
AuthorsAmunts A / Itoh Y / Naschberger A
Funding support Sweden, European Union, 4 items
OrganizationGrant numberCountry
Swedish Research CouncilNT_2015-04107 Sweden
Knut and Alice Wallenberg Foundation2018.0080 Sweden
European Research Council (ERC)(ERC-2018-StG-805230European Union
European Molecular Biology Organization (EMBO)H2020-MSCA-IF-2017European Union
CitationJournal: Nat Commun / Year: 2020
Title: Analysis of translating mitoribosome reveals functional characteristics of translation in mitochondria of fungi.
Authors: Yuzuru Itoh / Andreas Naschberger / Narges Mortezaei / Johannes M Herrmann / Alexey Amunts /
Abstract: Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each ...Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each other is not understood. We report models of the translating fungal mitoribosome with mRNA, tRNA and nascent polypeptide, as well as an assembly intermediate. Nicotinamide adenine dinucleotide (NAD) is found in the central protuberance of the large subunit, and the ATPase inhibitory factor 1 (IF) in the small subunit. The models of the active mitoribosome explain how mRNA binds through a dedicated protein platform on the small subunit, tRNA is translocated with the help of the protein mL108, bridging it with L1 stalk on the large subunit, and nascent polypeptide paths through a newly shaped exit tunnel involving a series of structural rearrangements. An assembly intermediate is modeled with the maturation factor Atp25, providing insight into the biogenesis of the mitoribosomal large subunit and translation regulation.
History
DepositionApr 30, 2020-
Header (metadata) releaseNov 11, 2020-
Map releaseNov 11, 2020-
UpdateNov 11, 2020-
Current statusNov 11, 2020Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0292
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.0292
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-6ywv
  • Surface level: 0.0292
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_10977.png

Downloads & links

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Map

FileDownload / File: emd_10977.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationConsensus map for the assembly intermediate with bound Atp25
Voxel sizeX=Y=Z: 1.06 Å
Density
Contour LevelBy AUTHOR: 0.0292 / Movie #1: 0.0292
Minimum - Maximum-0.2819625 - 0.48563343
Average (Standard dev.)-0.00014527274 (±0.0109234555)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 423.99997 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.061.061.06
M x/y/z400400400
origin x/y/z0.0000.0000.000
length x/y/z424.000424.000424.000
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ400400400
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS400400400
D min/max/mean-0.2820.486-0.000

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Supplemental data

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Mask #1

Fileemd_10977_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_10977_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_10977_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Assembly intermediate of the mitoribosome of Neurospora crassa

EntireName: Assembly intermediate of the mitoribosome of Neurospora crassa
Components
  • Complex: Assembly intermediate of the mitoribosome of Neurospora crassa
    • RNA: 23 S rRNA
    • Protein or peptide: 60S ribosomal protein L2
    • Protein or peptide: 60S ribosomal protein L3
    • Protein or peptide: 60S ribosomal protein L4, variantRibosome
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 60S ribosomal protein L6
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: 60S ribosomal protein L19
    • Protein or peptide: Ribosomal protein L1360S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal subunit protein L15
    • Protein or peptide: 60S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: Mitochondrial ribosomal protein
    • Protein or peptide: Aconitate hydrataseAconitase
    • Protein or peptide: Mitochondrial large ribosomal subunit
    • Protein or peptide: Mitochondrial ribosomal protein subunit L23
    • Protein or peptide: KOW domain-containing protein
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 54S ribosomal protein L4, mitochondrialRibosome
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Mitochondrial ribosomal protein subunit L32
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Ribosomal protein
    • Protein or peptide: Mitochondrial large ribosomal subunit YmL35
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Mitochondrial ribosomal protein L43
    • Protein or peptide: 60S ribosomal protein L3
    • Protein or peptide: 50S ribosomal subunit L30
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Mitochondrial ribosomal protein L44
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: RNase III domain-containing protein
    • Protein or peptide: 60S ribosomal protein L20
    • Protein or peptide: Mitochondrial 60S ribosomal protein L25
    • Protein or peptide: 54S ribosomal protein L31, mitochondrialRibosome
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: ATPase synthesis protein 25, mitochondrial
  • Ligand: MAGNESIUM ION
  • Ligand: NICOTINAMIDE-ADENINE-DINUCLEOTIDE
  • Ligand: SPERMINE
  • Ligand: POTASSIUM IONPotassium
  • Ligand: ZINC ION

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Supramolecule #1: Assembly intermediate of the mitoribosome of Neurospora crassa

SupramoleculeName: Assembly intermediate of the mitoribosome of Neurospora crassa
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#43
Source (natural)Organism: Neurospora crassa OR74A (fungus)

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Macromolecule #1: 23 S rRNA

MacromoleculeName: 23 S rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 1.114857375 MDa
SequenceString: AAAUGUAAUG GAUAUAAAGC UUAUGUUUAU AUAUAUAGAC AUAUAUAAGU AUAUAAAGAG ACUACUACCA AUAGCUACAC UAUGUAUUA AGGAGAGUAU AACUUAAUUU AUGUUUAUGA UUUUAUCAUA CCCCUAAAAA UGACACCGAG GAGCAAGGGU C GGGUUAGC ...String:
AAAUGUAAUG GAUAUAAAGC UUAUGUUUAU AUAUAUAGAC AUAUAUAAGU AUAUAAAGAG ACUACUACCA AUAGCUACAC UAUGUAUUA AGGAGAGUAU AACUUAAUUU AUGUUUAUGA UUUUAUCAUA CCCCUAAAAA UGACACCGAG GAGCAAGGGU C GGGUUAGC AUCCUGGUUC GUACACCUUG GUGACCUAGG CUAGUACCAG GUCCCCCUCU AAGGGACUUG UCCCCCUCUA AG GGACUUG CGUCGGUCCU AUCCUAGGCC GAAUAGGUGA AUAAAUACUU ACGGACGGCC UUGGUCUGUC CUAGAGGUUA UCA ACAUAU GAACUCUUAG AGAAAUUACU UAAUAAACGA AGUGAAUUGA AAUAUCUUAU UAACUUCAGG AAAAGAAAUC AAAC GAGAU UCUAUGAUUA GUGUGAACGA AAAUAGAGCA GCCUAUUAAA AUAAGUAAAA UGGCUUUAAA GCUGUUUGAA UAUUG UGGG GAACCUUCCU CAAAGGCUAA AUAUAAUACA UGAGUUACAG AGAAAAGUAC CGUGAGGGAA AGCUUUGAAA UAGUAG UUU UAUAAGCAGC UCAAGCAAUA AGAAAGCGAG AGCGUACCUU UUGCAUAAUG GGUCACCAAG UUAAUUUUAG AUGCGAG CG AAUUUAUUUA UGUUUUUACU GAUUAAACAA UAUAAUGAAU CAUAAUUAUU UUUGUAACGA GUAUUAGUAU UAAAUCUU A AUUUAAUAUU AGUAUAAGUU UUCAGUAUGG CGGCUACAUA GCAUAAUCUA UGCAGCCAGC CAAUAAUUGG AUUUCCAAU CCAAUUUCGG UAAUAAAUAG AUGUGCAUAG UUAAACCGAU CAUUAAAAUA AUGAAUAGUG UCUAAAGUUA GACCCGAAGC CUGGUGAUC UUACUAUAGU CAGGACUAUA AAGGUCCGAA CGGGUUAUCG UUGCAAAGAU AUCCGAAGAA CUAUGGUAAG C GAGUGAAA GACAACACUG ACUAGGAUAG CUGGUUUUCU GCGAAACCUA UAAUAGUAGG CAAUUUAAGU AACAUCUUAG UA GGUACAG AACUUAAUCU CAGACAAGAU GUAGAUUUUC AUACCUAUGU UUAGGUAUGA AAUGCAUUUU UUUUUGUAUA CAU CGGGGG AUCGUGAAGA UUUUAUCGGU GAGUAUGUAG ACUCGGAAUG ACAAAGAUGA AUCUUGAAUA AUCAGACAUA GAAU GAUAA GGUUGUAUGU CAAAAGGGAA ACAGCCCAGA ACAAGAGUUA AGGUUCCAAA AUUAUUAUUA AGUGAAAUAA AGAAA GUUU UUAUAUAAGU CGACAAGAAG AUGGGCUUGG AAGCAGCCAU AAUUUAAAGA UCUCGUAACA GAGCACUUGU UAAAUC UUA AAAGCAUCGA AAAUUUAACG GAUCUAAAUA AUAUACCGAA ACCUUGUCCA UAUGUAACAU UAGUAAUAAU AUGCUAU UA AUGUUAUUUG AUGGGGUAGC AGAACGUUGA GUGAAUCUUA GAUUUUUUUU UUAUAACUAA AUAUAGAUGA UAACUCAA G UGAGAAUGGU GACAUGAGUA ACAAAAAAGA GUUUAAGGUA CCUAAAAGGU AUCUUAGAGU CUCGCCUAAA GCUUAUGGC UACGUCAAGU AACGGCCUCU AAGUUUAUAA UCUGAAGAUU AUGACGAUGA GAAAAUAACG CGCAGAAGUG CGCUGCUUUG AUACUUAUG GUACCAACAU UUAAAAGUGA AAAUUGUGCA GGAAGGAUCA GUAUCCUUUC AUUCUUAUGU GGGGGAGUGG A CAAAACUG AACAGAGUGU AUCUGAACAC AGAUGAGUCC ACACCCCCCC CCAUGUAAUG AAUGAAUGAC AAACCGUACC UA GAAUCUG AAACAAGUAA GCUAGUAGAG AAUACGAAGG CGUGAAUGAG AUAACAAUCA UAAAGGAACU CGGCAAACUA ACU ACCGUA ACUUAGGGAU AAGGAGAGCU CAUUAGUCUC GAUUAAUACG AGUAAAAAGG AAGAAGCAUG GAAUAUUGUU GUAC GACUG UUUAAUUAAA ACAAAGCACU UUGCAAAAAG ACGAUAAGUC UAAGUAUUGA GUGUGAUUUC UGCCCGAUGC CGGCU GGUU AACGAAUUUU CUAAAUUGAA AAAAAAUUUG GUUUCAGAGG AACCCCCGGU UAAUGGCGGC CUUAGCGUGA GGGUCC UAA GGUAGCGAAA UGCCUUGGCC GUUAAAUGCG GUCUUGCAUG AAUGAUGUAA CGAUACAACA GCUGUCUCUA UGAUUGA CU CAGUGAAAUU GGAAUAACUG UGCAGAUACA GUUUACCUCU AGUUAGACGA GAAGACCCUA UGCAGCUUUA CUGUUACU A AUUAUUGAAU ACGAUUCUGA AAAUUUCCAG UGUAAAAGGU AAUCGAUAAG AUAUAAUUGA AACACCUUUA UUUUUCUAU CGUAUUAUUA AACCUUAAAU UAAGGAACAA UUGUUAGAAG ACAGUUUAUG CGGGGCACAG GCCCCAUAAA GAGUAAAUGG GUGUGUCUA AAAUUUAUAA AUUUAUGUUU GCAAUUUUUU AUAGUGAUUA UAUAUCAAAU CAUCUUUAUG CUAUUCAUAG A GUGUAUUU AUUAUAUUCC UUGGGUACAG UAUAAAAAUU AUAUAUGUAU UAAUUUACAU AUAUUUUUUC UAAGAAAUUA GG UAAGAUU UUGUUUAUAG AGAAAUUAGA UGUAAAAAAA AAAUCUUAUG AGGGCGGUAU UUAAUAAUCC GCUUCUAAUA UUU UUUUGU AGUUAUUAUU AUAAAUUUAA UAAUAAUCAU GUUUAUUACU UAAAAAGCUU AAUGGCUUAA UCUUGCCUUA CUGU UUGAU UAACAACAAA UCUUACAGUC GCGUAAGCGG GGCAUAGGAU CACAAGAUAC AAAAAGGAAA GAUCUUGGAU UUUUG GAAA AGCUACGCUA GGGAUAACAG GCUAAUUUGC GCAAGAGUGU ACAAAAUGAG UGCGCGGUUU GGCACCUCGA UGUCGG CUU GACUAAUCCU CAUGGAUGCA GAAACUAUGU AGGGUACGAC UGUUCGUCGA UUAAAAAGUU ACAUGAGCUG GGUUAAA UA CGUCGUGAGA CAGUAUGGUU UCUAUCUUCU AGAGGGAAUU AGAAUAUAAU AAGGAUUAAC CUUUGUACGA AAGGAACA U GGGGUACUAU UGUUAUACCU AGUUGUAUAA CAGUUUUAUU AACCUCUGGU UUACCUGUUG UUUAUGUGCC UUAUAUUAA UUUCAUGUGU GAUGCUCCGC AAGGAUAUUA CAGGGAUGUU ACCGUCACUU GAGUAAAUAC AAUAGCAUAA GCAUGGCAGG AAAGCUAAG UUAGUCAAAA AUAAGUGCUG AAAGCAUAUA GGCACGAAAU UUACCUUAAG AUAUUUCUUA AAUAUACGUA A GAAAAUAU UACGUUAAUA GGCUUAGUUU GUAAUAAUCU AGAGAUUUUA AGGAACUAAG UACUAAUUUU AUAAAAAACU GA AUGAUUA AUAUAUCUUA CAUUUUC

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Macromolecule #2: 60S ribosomal protein L2

MacromoleculeName: 60S ribosomal protein L2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 42.260766 KDa
SequenceString: MLQPQFRPLL AGTASFAQTV LGRTVARCYA TKAATQSASS TSTSNTSKDA KAKIVTPYVR DAGMMRTYKP HTPGIRHLKR PINDHLWKG RPYLPLTFPK KGQSKGGRNH SGRVTVRHRG GGHKRRIRMV DFERWIPGPH TVLRIEYDPG RSAHIALVKE E ATGRKSYI ...String:
MLQPQFRPLL AGTASFAQTV LGRTVARCYA TKAATQSASS TSTSNTSKDA KAKIVTPYVR DAGMMRTYKP HTPGIRHLKR PINDHLWKG RPYLPLTFPK KGQSKGGRNH SGRVTVRHRG GGHKRRIRMV DFERWIPGPH TVLRIEYDPG RSAHIALVKE E ATGRKSYI VAADGMRAGD VVQSYRSGLP QDLLDSMGGV VDPGILAART CWRGNCLPVS MIPVGTQIYC VGSRPDGKAV FC RSAGTYA TIISKEEETR EDGTKVMTGK FVNVRLQSGE IRRVSKDACA TVGIASNIMH HYRQLGKAGR SRWLNIRPTV RGL AMNAND HPHGGGRGKS KGNRHPVSPW GTPAKGGYKT RRKSNVNKWV VTPRVRNMGV RRNKKTT

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Macromolecule #3: 60S ribosomal protein L3

MacromoleculeName: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 41.262527 KDa
SequenceString: MAPRLPARCW RQLSLVERSA THTTTTAAAS SLLLAGRTTP TFSASSPSTV FPSLLPQITK RGVKYGWSTL PKRSRPTRFN QVTQGLPAP TSGPAAALKR REKTTPLRTG VLAVKKGMTV FMGRTGARIP CTVLQLDRVQ VVANKTRAKN GYWAVQVGLG E RRAENVGA ...String:
MAPRLPARCW RQLSLVERSA THTTTTAAAS SLLLAGRTTP TFSASSPSTV FPSLLPQITK RGVKYGWSTL PKRSRPTRFN QVTQGLPAP TSGPAAALKR REKTTPLRTG VLAVKKGMTV FMGRTGARIP CTVLQLDRVQ VVANKTRAKN GYWAVQVGLG E RRAENVGA PQLGYYEAKG IPPKQTLAEF KVRNQDGLLP VGVQLFPDWF HVGQVVDVRG ITRGMGFAGG MKRHGFAGQE AS HGNSLNH RTIGSVGGSQ GSGSRVLPGK KMPGRMGAQQ HTVQNLPILM VDNELGIVVV KGAVAGHKGA VVKVQDAVKK APP PEEFVE ATKQLLNERF PDAEEKLQAA RKLHLELKEA RRQGLIDSLI KNGLTEKADG NAAVEASA

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Macromolecule #4: 60S ribosomal protein L4, variant

MacromoleculeName: 60S ribosomal protein L4, variant / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 36.402785 KDa
SequenceString: MAGKGLKSLN EAMNALSIAS KSCRALPMRQ SSILPCRRSM ASVATPPAAN ITRSVSEPWQ PITNVPVTVY SFPELEPRSL ESYSARHLH LPLRRDILHL AVIYEGDSTR RGMASTKTRY EVHGSHKKMS PQKGTGNARR GTRQSPLMKG GGKTFGPKPR D FSTKLNKK ...String:
MAGKGLKSLN EAMNALSIAS KSCRALPMRQ SSILPCRRSM ASVATPPAAN ITRSVSEPWQ PITNVPVTVY SFPELEPRSL ESYSARHLH LPLRRDILHL AVIYEGDSTR RGMASTKTRY EVHGSHKKMS PQKGTGNARR GTRQSPLMKG GGKTFGPKPR D FSTKLNKK VYDLAWRTAL SYRYKRGELI VTEDGLDLPL PNDFLWLAGG GKLSRELEDG YVRKWVHEFM TSLNWGKEAG RT TFITGDK RPNLFTGFEL AGAEGRALEL WDVDVKDLLE TGRIVIERSA LKEMIEDHQS DLVTRVAVQG LRQKGPNLGE VLV RAPRY

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Macromolecule #5: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 39.170875 KDa
SequenceString: MASLRGVSRS ARALQPFSAQ FAVRRCASTQ TGAGAAAATP KSNIPDLAEL ETRSALDAPI PSEEDKKEFR PWKRAADRKA RLPSSRYQY HPPKYNRGPL HPIQSPPSSD PIARDFVPGP FNMPRLKETF RTVMASDLMT LAYIHTPPGT PKKEPTERLR A WEGDSPYF ...String:
MASLRGVSRS ARALQPFSAQ FAVRRCASTQ TGAGAAAATP KSNIPDLAEL ETRSALDAPI PSEEDKKEFR PWKRAADRKA RLPSSRYQY HPPKYNRGPL HPIQSPPSSD PIARDFVPGP FNMPRLKETF RTVMASDLMT LAYIHTPPGT PKKEPTERLR A WEGDSPYF ANRARRAPRG APELPIRERD ISFRNIPEIK EITVSTFVPL GLKNPDLLIV ARAVLLAMTG TMPEMTRSKN NV VQWQLQA NKPAGCKTTI YGNAAWEFMD RLIHLVLPRI KDWKGVPAST GDGSGNVQFG LNPEDVQLFP EVECNYDMYP AKM IPGCHI AIKTTATSDR QAKLLLQSLG VPFYSN

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Macromolecule #6: 60S ribosomal protein L6

MacromoleculeName: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 28.629158 KDa
SequenceString: MFAPSRRRVL EAVASSSSSP VVTLPGFLVP AFQQTAGAAA RRNFSATTTR PSKLGRTPLS IPPGVEITIG EPFVKRDMTQ WKQQPKRKI TVQGPLGQLE MDIPDFIKID HDAEARRATL SVANRDEKEQ REMWGTTWAY LNRFIMGVSE GHTAVLRLVG I GYRATIDT ...String:
MFAPSRRRVL EAVASSSSSP VVTLPGFLVP AFQQTAGAAA RRNFSATTTR PSKLGRTPLS IPPGVEITIG EPFVKRDMTQ WKQQPKRKI TVQGPLGQLE MDIPDFIKID HDAEARRATL SVANRDEKEQ REMWGTTWAY LNRFIMGVSE GHTAVLRLVG I GYRATIDT RPEKEEYPGQ QFVCLKLGFS HPVEMGVPKG MKASTPQPTR ILLEGINREQ VMTFAADIRR WRVPEPYKGK GI FVNGETI KLKQKKIK

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Macromolecule #7: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 37.363766 KDa
SequenceString: MSLRLSRPAV RGLGSAIKSS RISSRSAALL VPSTSTSAFS TASPQRAAAA GHLRLPDDYV PPTQPPSARP VDTRKSQLLR TYTSMLRST PLMLIFQHNN LTAIEWAAIR RELSLALSNV PVPEGAPDIT SKIHLQVVRT RIFDVALKTV EFFDPSTVEP T TATTATGT ...String:
MSLRLSRPAV RGLGSAIKSS RISSRSAALL VPSTSTSAFS TASPQRAAAA GHLRLPDDYV PPTQPPSARP VDTRKSQLLR TYTSMLRST PLMLIFQHNN LTAIEWAAIR RELSLALSNV PVPEGAPDIT SKIHLQVVRT RIFDVALKTV EFFDPSTVEP T TATTATGT KVPATYNHDL SKHAWKAVKE ATKNTEAVEK TVYGQLAPLL VGPVAILTLP SVSPAHLGAA LSVLAPSPPA FP APSRKKN PGYYDLTCQS GLQKLLLVGG RIEGKAFDYD GIKWVGGIEN GIEGLRAQLV HMLQSAGMGL TSVLEGAGKS LWL TMESRR SVLEEEQNPK KEGEGEEEKK E

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Macromolecule #8: 60S ribosomal protein L19

MacromoleculeName: 60S ribosomal protein L19 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 16.629375 KDa
SequenceString:
MSKAAKGGAG GVEQVVKLIV GAGQASPSPP VGPALGSKGI KSMDFCKEFN ARTAHINTGT PMPVRVTVRP DRSFHFDVRT PHTSWLLLN AAEAPIGKGG KRKGASNPGK EVVGTVSLKH VYEIAKIKQS ELRLSGLPLE GLCRAVIYQA RSIGINVIP

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Macromolecule #9: Ribosomal protein L13

MacromoleculeName: Ribosomal protein L13 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 20.334576 KDa
SequenceString:
MSQTVGATRL AYSRVWHHIS AVTPHPTLST IKAPSEAITP PSLGRLASRI ATILMGKHKP IWDPSTDCGD YVVVTNCAGL YTTGHKKWR KTYYRHNTRP GSLQAITMDA LMEKHGGAEV LRKAVSGMLP KNRLRDKRLA RLKAFEGDAH PYKENLVRFG G KVVGAPGW EEAVKAIREA DMERL

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Macromolecule #10: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 14.13563 KDa
SequenceString:
MIQLKTMLNC IDNSGAALVE CAMVVGQKRH ASIGDRIVVV VQKQRGADSA GMAASSAATK VKRGDIRHAV VVRTKQKVQR RDGSVVRFD DNACVLINKA GDPIGSRING VVGQELRKKK WSKILSMAPM QA

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Macromolecule #11: 50S ribosomal subunit protein L15

MacromoleculeName: 50S ribosomal subunit protein L15 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 33.951125 KDa
SequenceString: MPPRLPLAQA ARCCQASLTA RPSVPASSPT SSLISLFAAL SVQTRSASIL ASLSDNRGAY HKRIRKGRGP SSGYGKTAGR GTKGQKAHG HVKPWFQGGQ TPLIVSHGRK GFVNQFAADM SELNLEKLQE WIEAGRIDPT KPITPKEIIK SGIIGSSIKD G IKLLGRGK ...String:
MPPRLPLAQA ARCCQASLTA RPSVPASSPT SSLISLFAAL SVQTRSASIL ASLSDNRGAY HKRIRKGRGP SSGYGKTAGR GTKGQKAHG HVKPWFQGGQ TPLIVSHGRK GFVNQFAADM SELNLEKLQE WIEAGRIDPT KPITPKEIIK SGIIGSSIKD G IKLLGRGK ESFKIPVTIT VSRASASAIE AIEAAGGKIV TRFYTKESLK RLVEGKSLHT DKPLPVGKEH VEEILAQARS LK KKYYRLP DPTSRWDIEY YRDPAHRGYL SHQLAPGESP SLYFKVPTGG EKVKVVRADK VKASKTGDVA SEKLF

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Macromolecule #12: 60S ribosomal protein L16

MacromoleculeName: 60S ribosomal protein L16 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 27.443828 KDa
SequenceString: MKHNASSALL SAFQGLRISS SATPFRAASL ATSAVRRPIA PTPVSVASHV RLFSATAIQA GSWLEPNLNR KKKMMKGRPR VPTGGSTKG TTVVWGDYGL RMRDHHRRIS AQQLKLAEDT IKQRLRGQKY RLYKRVACNV GVYVSGNEMR MGKGKGSFDH W ATRVAVNQ ...String:
MKHNASSALL SAFQGLRISS SATPFRAASL ATSAVRRPIA PTPVSVASHV RLFSATAIQA GSWLEPNLNR KKKMMKGRPR VPTGGSTKG TTVVWGDYGL RMRDHHRRIS AQQLKLAEDT IKQRLRGQKY RLYKRVACNV GVYVSGNEMR MGKGKGSFDH W ATRVAVNQ IIFEIRGQLH EQVIRDAFRL AGHKLPGLYE FVKKGDPPVV GITKLEDGLT VEDLKNPRKK LLMPEITQSA AS TSSTAAP PS

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Macromolecule #13: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 22.181545 KDa
SequenceString: MAGAHVKYRH LSRSSAHRQA LLRNLVTSLI KNETIHTTYP KAKEAQRLAE KLITLSKRDT ETARRSAQGI LYTPDQLLPK LFGEIRERY ANRPGGYTRV LRTEPKDQYS QAPSAILELV DGPKDMRFAM TAATVARDRE QKTESTDLTK KNMDKVTRFR K DGMRAFED ...String:
MAGAHVKYRH LSRSSAHRQA LLRNLVTSLI KNETIHTTYP KAKEAQRLAE KLITLSKRDT ETARRSAQGI LYTPDQLLPK LFGEIRERY ANRPGGYTRV LRTEPKDQYS QAPSAILELV DGPKDMRFAM TAATVARDRE QKTESTDLTK KNMDKVTRFR K DGMRAFED MVASLRDMKF TAGAINPKEW KKLDR

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Macromolecule #14: Mitochondrial ribosomal protein

MacromoleculeName: Mitochondrial ribosomal protein / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 28.235305 KDa
SequenceString: MNVTASLSRR PLGCLKAGLC QSLIRGYATA VAASTTQSTG ALPDHNFFRI ADKHTKKTRT AFAVFTPPKS PTTLPPSSVV TKSKAFKIA NSKLPPLMTK PPSDPMPLLT QQQIARMDPT GARTALFSKA RHAARVGDVL MVTHRRGGEP FAGVCLAIRR S GIDTAILL ...String:
MNVTASLSRR PLGCLKAGLC QSLIRGYATA VAASTTQSTG ALPDHNFFRI ADKHTKKTRT AFAVFTPPKS PTTLPPSSVV TKSKAFKIA NSKLPPLMTK PPSDPMPLLT QQQIARMDPT GARTALFSKA RHAARVGDVL MVTHRRGGEP FAGVCLAIRR S GIDTAILL RNHLGKVGVE MWYKIYNKNV AGIEIIKRRA KRARRAKLMY MRQPKHDMGS VEQYVFAWKK MRKVLSSKGL TG GVGGGGG KQKGQESKKK N

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Macromolecule #15: Aconitate hydratase

MacromoleculeName: Aconitate hydratase / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 23.953027 KDa
SequenceString: MSRALLRSVL ELRTPVTRLP PSFLLPFRPA ARFLHQTAQQ VEPTSQSDAA AAAATLLKAS PPKVTTATTP EAPAAVPTST PFSQQPPIS QQVKELLPVL AAQPGHYTTI HIHGKPYLVT EGDTVKLPFK MPGVAPGDVL RLNRASVIGS RDLTLQGAPY V DERLFECR ...String:
MSRALLRSVL ELRTPVTRLP PSFLLPFRPA ARFLHQTAQQ VEPTSQSDAA AAAATLLKAS PPKVTTATTP EAPAAVPTST PFSQQPPIS QQVKELLPVL AAQPGHYTTI HIHGKPYLVT EGDTVKLPFK MPGVAPGDVL RLNRASVIGS RDLTLQGAPY V DERLFECR AIVMGTESEP MRVMIKKKRR CRKKKHVFSK HKYTVLRINQ LKINDVSSL

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Macromolecule #16: Mitochondrial large ribosomal subunit

MacromoleculeName: Mitochondrial large ribosomal subunit / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 41.565996 KDa
SequenceString: MSLNVPSRRL LKSAPSSANA LVPALQSLSI NTRSIHTTPS QQWSLFGWGK QKVEERNKVK EELKQSELAR TRKPTQEEIF ERVRGRLEG DSIFADTTPS SQLAAEQDKH KDWATASAEK KLAAAHAKDQ TPTGISLKKD HLVRVVDPDP RSRVRWERKM V IRKLQRGT ...String:
MSLNVPSRRL LKSAPSSANA LVPALQSLSI NTRSIHTTPS QQWSLFGWGK QKVEERNKVK EELKQSELAR TRKPTQEEIF ERVRGRLEG DSIFADTTPS SQLAAEQDKH KDWATASAEK KLAAAHAKDQ TPTGISLKKD HLVRVVDPDP RSRVRWERKM V IRKLQRGT DPWSVEPKAE RIARTERKLV YKTGYLPTSV KKLVHLSRQI RGKTVSEALV QMQFSKKKMA KEVKTELLRA EA KAIVTRG MGLGKAAAAA AQKETGAEPV KIQTKDGKHL EIRDPTRIYV AETFVNKGFT RGVELDYRAR GRVFKMNKPT TTM TVVLKE EKTRIREHQE RVAKKLRQGP WVHLPDRPVT SQRQFYSW

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Macromolecule #17: Mitochondrial ribosomal protein subunit L23

MacromoleculeName: Mitochondrial ribosomal protein subunit L23 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 26.005465 KDa
SequenceString: MASVAKTGAE ALRNAPFRVG KKQIFLPNHV ITFVRPLPKQ PPNLATFIVP LEFNKLDLRD YLYHVYNVEV TGVRSFVNQR MHEQRHGDV GNWRRPKSQK MMIAELAKPF VWPKPPAEDA REAFDYAMWK KQKTAQEQDT KFQGIRAQGE VVPPSQFEVT K DRKAIKAR ...String:
MASVAKTGAE ALRNAPFRVG KKQIFLPNHV ITFVRPLPKQ PPNLATFIVP LEFNKLDLRD YLYHVYNVEV TGVRSFVNQR MHEQRHGDV GNWRRPKSQK MMIAELAKPF VWPKPPAEDA REAFDYAMWK KQKTAQEQDT KFQGIRAQGE VVPPSQFEVT K DRKAIKAR QSKFLSGEET WAPGKVNAFL NSKIVPEEEG WSEVEENLPL DESAESAAEE SSSKGSETRQ

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Macromolecule #18: KOW domain-containing protein

MacromoleculeName: KOW domain-containing protein / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 45.460117 KDa
SequenceString: MDKLLRRVRM AEGMVARRAQ RKNALLKRIT ERKQNKKNGE AFTEAIQQRK AAVEARNEDW MLGPLAPRRE LDEITLSNGN FFGSLSPTR ALLESEVSEE ERKARVAWCG SPKFLCIAPG DRVVVIEGHH KDLIGTIEKL NTRNMTVEIQ SEKLKTNTTV P QFMQNDAD ...String:
MDKLLRRVRM AEGMVARRAQ RKNALLKRIT ERKQNKKNGE AFTEAIQQRK AAVEARNEDW MLGPLAPRRE LDEITLSNGN FFGSLSPTR ALLESEVSEE ERKARVAWCG SPKFLCIAPG DRVVVIEGHH KDLIGTIEKL NTRNMTVEIQ SEKLKTNTTV P QFMQNDAD KPVTQIYARL PISSVRLVHP LKDPQTGEYR DVIIRELRPR NIVHDRPTRT RSMRRFVPGE NIIIPWPKQE PI KREDQPA DTLRIDVDEK TFVPTLFRPP APQQVLDELR NKYSIFRTRH TPEYIAKKEQ EEQEKEAKKS AAKAMLTPVQ EYN RKQREL RRARGQPALT EEMLAKIGEV VARNKLGHHQ APKVKEETVA QIEKAVEQLS LGGGQEDAAT TTSPEQPKVV

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Macromolecule #19: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 49.549199 KDa
SequenceString: MHLAQLRQPL TRAAANSCHS VCRLPTTHSS VSATAILSEQ FAHLRIGPSS SNVAVEGRRY ASVKAQGAYR LKPKRTIPKK LGAKKTGDQ YVIPGNIIYK QRGTLWHPGE NTIMGRDHTI HAAVAGYVKY YRDPQLHPDR QYIGVVFNRN DKLPYPKDAP R KRKLGLVA ...String:
MHLAQLRQPL TRAAANSCHS VCRLPTTHSS VSATAILSEQ FAHLRIGPSS SNVAVEGRRY ASVKAQGAYR LKPKRTIPKK LGAKKTGDQ YVIPGNIIYK QRGTLWHPGE NTIMGRDHTI HAAVAGYVKY YRDPQLHPDR QYIGVVFNRN DKLPYPKDAP R KRKLGLVA VPRKVEEVEK PTMSASGLPL FVTRHETIEI PPPAVTTPAA AGKAVKGQGA RVSASGAAAV PASSSTISAS AS SNSNNGG SSVIAELIKE KLAARAEYNA RQSALRKLQQ QKMLARRGTR VLRLMNNYSY RETNWEIGRL IGDPGSVPGT EKV GSRKAK FRARRRRRNT FLLGIKERKL AKADRREEYR RRVREKREQR LVQRKEFLAK QREAKKAREG GAAAEKSEKK EVKA EKPAA AAPKVEKPKA PEAAKKESKP KVEEKKAAAA EPKKDSKTEK KD

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Macromolecule #20: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 31.501131 KDa
SequenceString: MSSFLVRPFC LRATTTTTTT TTTTALVQQQ LRALTTTTAL TYHQPRVPSS AIPIPNVSGR VPLPDSTPVP EDFKIEIPSY PYGPRRVYH QSNTGLYGSA LIRFGNNVSK RNEIKTRRKW RPNVQQKRLW SKSLGVFVRT RVTTRVLRTI DKVGGLDEYL L GHKAARVK ...String:
MSSFLVRPFC LRATTTTTTT TTTTALVQQQ LRALTTTTAL TYHQPRVPSS AIPIPNVSGR VPLPDSTPVP EDFKIEIPSY PYGPRRVYH QSNTGLYGSA LIRFGNNVSK RNEIKTRRKW RPNVQQKRLW SKSLGVFVRT RVTTRVLRTI DKVGGLDEYL L GHKAARVK ELGPWGWMLR WRIMQTPEIR ERFAKEREAL GLPPRREEDE EKSLEEQLRE FQVAGIQFAE GKAPKTKGQL KE EADRLIK KSETEEFGLG QEEDLFMKEE PKPTKMA

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Macromolecule #21: 54S ribosomal protein L4, mitochondrial

MacromoleculeName: 54S ribosomal protein L4, mitochondrial / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 29.64526 KDa
SequenceString: MASPAALRPS MGAIMQTCRS AATAPKVAVA VPVRALSTSA ALLKRHKYPT ARVTRDNSKQ RGESALRKSG TRWKLSVSDE PLPEPVPRE ELPPIQVDEN HGLWDFFYDR ETVAMAPLEH TKHGRAWTVS ELRKKSWDDL HRLWWVCVKE RNRIATANWE R TKSELGFG ...String:
MASPAALRPS MGAIMQTCRS AATAPKVAVA VPVRALSTSA ALLKRHKYPT ARVTRDNSKQ RGESALRKSG TRWKLSVSDE PLPEPVPRE ELPPIQVDEN HGLWDFFYDR ETVAMAPLEH TKHGRAWTVS ELRKKSWDDL HRLWWVCVKE RNRIATANWE R TKSELGFG LAEANERDRN VKQTMRGIKH VLTERFYAWE DAVKLAEQDP EIDLSNPENP YTPSTFLEAE ETAEGAEASE TQ STTTEID PTTIPSSKSQ AEAPRV

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Macromolecule #22: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 18.196393 KDa
SequenceString:
MSFFRITLHR SAIGLPKRTH GVLAALGLRR RNQTVFHPVE PQFAGMLMKV KELVRVEEVP HRLTKRELKD ERKPDAGFVV EKQVRRFIP GVGVQDEVDF SKVVEELKAQ KVENVEGVEK MVVEGGAVVR DGKRAGDLGV RTRSDWEVIG KMKSVLPKVK A L

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Macromolecule #23: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 23.766729 KDa
SequenceString: MSSKLPTTLL RRPSALPSTT TYTAYSASRP TPPSCTAHGA QGQQIRNATF VPRHRRPYQF TQLVQLSDGS TFTVRTTMPT ALYKSAKDS RNHLLWQPSD KSLKNVELDE AGKLAAFRER YGRGWDLDAK MTPEEEAAAA AALAAGGGAG VPGGKAAKKA A EEALLAKK ...String:
MSSKLPTTLL RRPSALPSTT TYTAYSASRP TPPSCTAHGA QGQQIRNATF VPRHRRPYQF TQLVQLSDGS TFTVRTTMPT ALYKSAKDS RNHLLWQPSD KSLKNVELDE AGKLAAFRER YGRGWDLDAK MTPEEEAAAA AALAAGGGAG VPGGKAAKKA A EEALLAKK KKEEEEAAKK AAEAEEADPF DSLTDLISGY ATENMNPGLN FKETRHYGKK K

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Macromolecule #24: Mitochondrial ribosomal protein subunit L32

MacromoleculeName: Mitochondrial ribosomal protein subunit L32 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 14.114779 KDa
SequenceString:
MAAMTAAAAA PAMRLFNPST FFQTLRTQRF GVPALNFAVP AAAISLLPSI PSLLEDIWEG ILRAVPKKKT SHMKKRHRQM AGKALKDVT HLNRCPACGN LKRMHHLCST CLGKLKGFMD RNGGSNAKAY

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Macromolecule #25: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 7.027574 KDa
SequenceString:
MAKKAKSRII NVRLISMAMT GYFYTFTRPR TSLPMSMLKY DPIVRRKVLF LEQKRKGRS

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Macromolecule #26: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 14.853288 KDa
SequenceString:
MSRIFSSALQ AVFKPSAFLP KTATRSFSIL PSLRPATLSS TPSTIFRAPN AITAPSASPS ATTGIDGEVL DLLSASSLIS SHPALSGLG SQIRCGPRPT MSGATRLVQK RRHGFLSRVK TKNGQKTLKR RLAKGRLRLS A

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Macromolecule #27: Ribosomal protein

MacromoleculeName: Ribosomal protein / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 13.960405 KDa
SequenceString:
MSNLFRSLAS SMRALSLAAP RATAVNTTKT VVSTHQTRCL SQGLLSRHIC TPMCSHNRPV AVCQSAKNGL QSKQQSRGMK VHSAIKKRC EHCKVVRRKA NKRQNGYLYI ICPANPRHKQ RQGYR

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Macromolecule #28: Mitochondrial large ribosomal subunit YmL35

MacromoleculeName: Mitochondrial large ribosomal subunit YmL35 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 50.749188 KDa
SequenceString: MSTCQQVARP LVRCLRQTGP GAATCSTRSV AVAAVRPFSS TPMRKEEGAT TTTTTSPVDS TTTPPPLPSA TEAKATVADT VVGGPELGS RRRRAALATT GNLPFEQLPY QCFQDARKIL QQDRAAKIAQ IVKETEKIKL IEARDASEFE GGEAAKQTRI K SLRKYIEE ...String:
MSTCQQVARP LVRCLRQTGP GAATCSTRSV AVAAVRPFSS TPMRKEEGAT TTTTTSPVDS TTTPPPLPSA TEAKATVADT VVGGPELGS RRRRAALATT GNLPFEQLPY QCFQDARKIL QQDRAAKIAQ IVKETEKIKL IEARDASEFE GGEAAKQTRI K SLRKYIEE LKILADINDP EVKRRFEDGR GDMTKPVYRF MAERRWRSMD YKIIAQRISQ FHVVPDLLPA FDPTMDVKLS FR GYQVSPG AILDSRVTEV APTLRMQVFD KGERLLTVVV IDSDVPDVTH DNFKRRCHFL AANIPWDPSK TVLSLRSVGD RVE GDVGKP WLPPFAQKGS PYHRLNVFVL EQKPGAKIDG EALKKHLENR ENFSLKGFRE KFDLEPVGFN LFRSEWDEGT AEVM ERHGI PGAEVEFKRQ KFASLKPPRK ARGWEAKRQK PKYKSLWKYV KRIA

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Macromolecule #29: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 41.496336 KDa
SequenceString: MVSATKKPVS AMSAKNSTRH RKVSHKVTKT IATAKSQVIS NKAMKIRTKS WKDMPSPKRR RRINAMVDEL RLQGNVKMPK PGKSTYKAG GGERVRLVKA ASTLPTPPLT PPVLSKPTGS ASQSKGDLKP KRPTPEKKRS VSKRDVERFF LSTQTSTRRR E NTPIHAPI ...String:
MVSATKKPVS AMSAKNSTRH RKVSHKVTKT IATAKSQVIS NKAMKIRTKS WKDMPSPKRR RRINAMVDEL RLQGNVKMPK PGKSTYKAG GGERVRLVKA ASTLPTPPLT PPVLSKPTGS ASQSKGDLKP KRPTPEKKRS VSKRDVERFF LSTQTSTRRR E NTPIHAPI MSSTFTSLRG LATRLFAGGA RPSPSSLLLP NKPATAALPT AIQHQQTASY ASKGKSGPPA GMFSGQKAGS KK SKGPKQV DPRIINILRH FAVLSPKRIP PPLRFGRNRY LRHWTIHRAW LLFRRQQREQ RERILMQQHQ SMSNACEELR NTE GPGTRE TGYLYRVAML KNGVYGLKSI PIEYASRALV ETPGRQAWNH EWKR

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Macromolecule #30: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 11.796603 KDa
SequenceString:
MQPTRILQGL KYRKLRLTTK DVNKGFYKGN RTGSMGTHTS YGTYKIDYTK VRTYVCPDLT GFKLTPFVSK TIRPVHDQFP GDKLGPKNP ATYLARWKSE NGLD

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Macromolecule #31: Mitochondrial ribosomal protein L43

MacromoleculeName: Mitochondrial ribosomal protein L43 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 15.126693 KDa
SequenceString:
MTVKALTQIS SAGRNGVGAF VLQCKKLDIH YSDWAGSSRG MNGFIKSLLP KFAAANPQIE FVVSPRPAKH PILMGHYING RTKAICVRN MEPLEILKKA ELLRDASGEK PQKFKKPVTS TNPSVRGVWS PYHGQGMAV

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Macromolecule #32: 60S ribosomal protein L3

MacromoleculeName: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 48.106484 KDa
SequenceString: MKRIATPSLT SRLLVAARSG VSPATAAIRS RSAISVRSQS TAALAQHDAS HDLNNDRFPP LEPLPPAAES LPSPLPERAL TSAKLAALH ARLNLSPKIP LQTLARTLVD ASADENPQFN NANLAFVGQT LINYHIAEWL LCKYPRLPQG ILFSAMKAYA G PKPLLQIA ...String:
MKRIATPSLT SRLLVAARSG VSPATAAIRS RSAISVRSQS TAALAQHDAS HDLNNDRFPP LEPLPPAAES LPSPLPERAL TSAKLAALH ARLNLSPKIP LQTLARTLVD ASADENPQFN NANLAFVGQT LINYHIAEWL LCKYPRLPQG ILFSAMKAYA G PKPLLQIA RSWGVDTAAV PGGEVDPGLL QFDALKPGVA ITNFGYKRTE LAYLEKFKWR RGMASRVVLD DDFGDVVRSD PK VAADLEK AMEEQDQDKT PDEEEAEMVA NEQDQDVSYD RYGNPDTRAA AERAHAYFVR AVVGAIYAHC GREAAKAFVK AHI MSRTLD IAKLFEFKYP TRELAALCAR EDFEPPVARL LSETGRQSRT PVFVVGIYSG SDKLGEGAAS SLDHARFKAA MNAL KAWYL YSPGENPRVP SDMLEEGAKP WTPAYIDMGE VISR

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Macromolecule #33: 50S ribosomal subunit L30

MacromoleculeName: 50S ribosomal subunit L30 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 41.322074 KDa
SequenceString: MSASSRGAAL LRSQQRSICL QCRNQTRVLA PAGVTSAPRR FYSAEASATA TATATATTTT TLPPPHPPVT TSTGTHAATS TSSQIYRIK SGVILTRPPL LTRDLTPFEE SFYFYQKRLN ERLTAPFRKD FYFKKDTAAD LDWRIKLKER HGVPAKDIGR Y NPRGRMAW ...String:
MSASSRGAAL LRSQQRSICL QCRNQTRVLA PAGVTSAPRR FYSAEASATA TATATATTTT TLPPPHPPVT TSTGTHAATS TSSQIYRIK SGVILTRPPL LTRDLTPFEE SFYFYQKRLN ERLTAPFRKD FYFKKDTAAD LDWRIKLKER HGVPAKDIGR Y NPRGRMAW NDEVLVGSQT SSRKHMVEKL LADAEMRVSE DGEEIPAEDR VPVEKPMPRR TEADEKGDVK RLDRALDKTL YL VVKKKAD KEGEEAKWMF PTGVVPTDEG LHETAARILA ESAGVNMNTW IVGRVPVAHH VVRPVFGQKD GALLKKGEKI FFL KGRIMA GQADLTDNLH DLVDFKWLTQ EELRSTLAEE YFHSVKGMFA ER

+
Macromolecule #34: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 18.180738 KDa
SequenceString:
MFRSTFFGLS RAIVQPATPL TVRAAFQSRF YSAAAAASQP TTTATTTTPL QQQQQQQPTT QPTTPIQTQT GAAPTESTKP VAKPYLVGR AWTQRLPVYH LAKRGGNKKL TQIKKVQGDG QALRRDLAQF LGLEVKEVRV KVPTGHLEVD GHRREEIVKF L DGLGF

+
Macromolecule #35: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 49.748516 KDa
SequenceString: MRRIPRIPST ASALCSSSSS VASTSAAPLR TLRAAADSSS ICQSCSFSTQ TSATRNRVWQ NTVQTQRRCA STVTETTPEA PVAAAAAET ATESTETVEQ QRKRKGGFFE TQRQVNLVLP GQPTRADAPE KIRDPNYEPA TSGAGLQEIG GMSDWWSKPE H FRDGGKQF ...String:
MRRIPRIPST ASALCSSSSS VASTSAAPLR TLRAAADSSS ICQSCSFSTQ TSATRNRVWQ NTVQTQRRCA STVTETTPEA PVAAAAAET ATESTETVEQ QRKRKGGFFE TQRQVNLVLP GQPTRADAPE KIRDPNYEPA TSGAGLQEIG GMSDWWSKPE H FRDGGKQF EYQGFAPQEK ITDPRLLKVI LRRALAEGLA LKKFGANPKN PADMASIIGN GDHWQRTVSV EMCRGENGEL SL KNESDLQ KVWILMRNAA EKTYYQREWQ EEINRLRSLG EKEQAKQLLE EGKKLGYRLK SEEGSLVKLT VDEAVELRKS WNN DWKEAI IRDPVVKFYA AKRIQKMTGH ILSDGKLTSI QTVANFMDAL VTPPKPKKLA EQIEQSSILP ELPNVKVYPR RVTP VDKER MVGRWKVIQK ELQKRELPVL GTGNHGKYVE LKWLGSKQ

+
Macromolecule #36: Mitochondrial ribosomal protein L44

MacromoleculeName: Mitochondrial ribosomal protein L44 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 11.128777 KDa
SequenceString:
MLTRFITDVS TRFNPFSAKA KAARLFLSFL PPNARSNGMN ITTQLLPRNS TETPLLYVKF KDGKEMNLDV ENMGIKSIVE EVDRHSRIL QKQADLNDG

+
Macromolecule #37: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 23.179602 KDa
SequenceString: MICRTCLRRA SGFARPAAAA ATRPMVAASS RAAFSTTFSV CTPPAATPAA AAPATSTAEA GLAPVPGAAT TEQAKAAISS CPAGTKLNG LNYFKNKADP VALPDEEYPE WLWRCLEVQK KTDDAADADA GDEFSKSKKQ RRLAMKRARA QEAKILASGD L EALAPKIP ...String:
MICRTCLRRA SGFARPAAAA ATRPMVAASS RAAFSTTFSV CTPPAATPAA AAPATSTAEA GLAPVPGAAT TEQAKAAISS CPAGTKLNG LNYFKNKADP VALPDEEYPE WLWRCLEVQK KTDDAADADA GDEFSKSKKQ RRLAMKRARA QEAKILASGD L EALAPKIP LQKQSVNLPG AVNGGVQDAV LADEKREELR KAMRKERKAK IKESNYLKAM

+
Macromolecule #38: RNase III domain-containing protein

MacromoleculeName: RNase III domain-containing protein / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 29.625195 KDa
SequenceString: MAALTPSRSV FASTCKTIAQ QCSRRPVAVQ QLAAVPVARQ VSSSSAVQQE QDASGTSSSQ QPRPRWSYTP ERMKGPGFSL NLVKDPRRK QWMVNSDPAK LDAFYDAFLG QGGSRMLSEE TKWLAVTHKS FDYGRRGYNT RLAFLGRQII ALETTRSILT S PVLNEPIV ...String:
MAALTPSRSV FASTCKTIAQ QCSRRPVAVQ QLAAVPVARQ VSSSSAVQQE QDASGTSSSQ QPRPRWSYTP ERMKGPGFSL NLVKDPRRK QWMVNSDPAK LDAFYDAFLG QGGSRMLSEE TKWLAVTHKS FDYGRRGYNT RLAFLGRQII ALETTRSILT S PVLNEPIV DKYGRTPYNH AALANIDKLI YTQPLDIMDK TKIARMGIDF GLLTVMRWKP RMPEDLESSG VVVVLNSTLF AI IGAISLE KGAAVAQRIV REKILKRLGA

+
Macromolecule #39: 60S ribosomal protein L20

MacromoleculeName: 60S ribosomal protein L20 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 25.439018 KDa
SequenceString: MEASLSLFRP VATCCRRVAL SSSSSSSQKA AAVAGISVRY QSTANRTKRM LNIPPHESFL NVPVEGDRII FNPPSSEASV YHTPFKFLP RSDPRRRANI YKLFKPPQAP ITTPESSSTD AAAADQHGDL PPVLYNPTKS YNVTPEQVEE IRELRAKDPK K YSVTYLSN ...String:
MEASLSLFRP VATCCRRVAL SSSSSSSQKA AAVAGISVRY QSTANRTKRM LNIPPHESFL NVPVEGDRII FNPPSSEASV YHTPFKFLP RSDPRRRANI YKLFKPPQAP ITTPESSSTD AAAADQHGDL PPVLYNPTKS YNVTPEQVEE IRELRAKDPK K YSVTYLSN KYNCTKVFIM MCTQAPREHQ EQHKLARART AENWGPRRAA AKLDARRRKE MLHRGEI

+
Macromolecule #40: Mitochondrial 60S ribosomal protein L25

MacromoleculeName: Mitochondrial 60S ribosomal protein L25 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 18.745025 KDa
SequenceString:
AATVKQHVQL ALSLPPRLRT FLARYPPASI LPAGADPETH KTPYQEESPN PFMPTKHPIT GKWHNPKYSL RRQAELVKLA QEHGVEELL PFTRKLNEEK LRKRVELGLR VKGTGVGEKV KGHKHERTLV AKMEKRREAM LAMPDLIREW KKVGKRNWTK F PK

+
Macromolecule #41: 54S ribosomal protein L31, mitochondrial

MacromoleculeName: 54S ribosomal protein L31, mitochondrial / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 13.131485 KDa
SequenceString:
MFGAFRFTNP LSGGLLWKIP WRMSKFQKLR QRRRLRAVDN VVATIENALA KKGETVKAVE RWRAEMPTEA EMLPKDKYTI FDRKEKRYR KGIHKLPKWT RVSQRVNPPG Y

+
Macromolecule #42: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 32.516111 KDa
SequenceString: MNSVTTSTIG RLAFGVSRLS AVTNVAGAVR HASTHPTTAI PKPTTAVLKT SPLAEETAVE LKSPSRLQLK SEPGNKGNPK GHGDRIWAF HHIEQGQVIY STKPVISHQH LIRQQPFTGK NLVPRKIRKD YWRPLAMIEL AGKDQGAVGR SVYQKLREFK K RHELDWAN ...String:
MNSVTTSTIG RLAFGVSRLS AVTNVAGAVR HASTHPTTAI PKPTTAVLKT SPLAEETAVE LKSPSRLQLK SEPGNKGNPK GHGDRIWAF HHIEQGQVIY STKPVISHQH LIRQQPFTGK NLVPRKIRKD YWRPLAMIEL AGKDQGAVGR SVYQKLREFK K RHELDWAN EAEEGRKLMH KSKRERGQEL NDQKPNSVAD MAAVLAGAGK GNLMWQVSRL GSSSELVGEI KKASGAVEKG AV VDVKRQL RRATVYWTNE QDKFHAREWS DNVSHEVGIP GEAEKKRMPI RMSSE

+
Macromolecule #43: ATPase synthesis protein 25, mitochondrial

MacromoleculeName: ATPase synthesis protein 25, mitochondrial / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Neurospora crassa OR74A (fungus)
Molecular weightTheoretical: 77.985422 KDa
SequenceString: MSATPAVLRA SACSACRFSA LRLFVSSFAS PRAPLPVART RVLSPTAPYS TFRPSPRLLA SPSSEEHAEP AAQEQPNKTE DAQAEASTE ANNEGGADVP WYLQVDAPTH PTLVHEPPPL PDIPEGSPKL MEPLVKFVSD ELGMDDLKLL DLREIDPPPA L GPEVLMLF ...String:
MSATPAVLRA SACSACRFSA LRLFVSSFAS PRAPLPVART RVLSPTAPYS TFRPSPRLLA SPSSEEHAEP AAQEQPNKTE DAQAEASTE ANNEGGADVP WYLQVDAPTH PTLVHEPPPL PDIPEGSPKL MEPLVKFVSD ELGMDDLKLL DLREIDPPPA L GPEVLMLF GTARSERHLH VSADRLVRWL RNRGVTAKAD GLLGRNELKT KLRRKARKAK LLGTTGLPSG ADDGITTGWI CV NLGTLGW SDMEMEFKDE KGVTSGFGVP QSGTTLVVQL MTETRREELA LEKLWSGILR RSLERQDKIA GKLPEASFTT SPD TVSGQS FSPSPIRSSG RNRKRPDRLY FSTSARQHAA AVDVSQTDLA TAMAGWEYSN ASPSTIDNLL AQDTDSKVQL LIQM QEYLS SLPRDQALSA VSLCEDGSPS TFMRLFNRAI ENLPSAQAWE ARLWVEKAAR ALQHPDHDLA RLGDLIQEMK LSGAA NVSR EKFVDLLRMI FAIPETTDAG VRQQASLSMD VIDMLFSRGE KVIEYDVVVA VIESLLQTRA RPPEARRLLS QFEDLL SEA QMDCPTEDEI IRLLDVYANH RAWDKFWNVW RVFPRHCERR TERMYTKVYE RIAAEDHQGM ATDALRWCVE EMWHEQP PV RVTAAMLKAL EACIRIADPE AESLAKDIDD RISDAQYMDR EFVRLWLTLH GSAAWGA

+
Macromolecule #44: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 44 / Number of copies: 144 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #45: NICOTINAMIDE-ADENINE-DINUCLEOTIDE

MacromoleculeName: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / type: ligand / ID: 45 / Number of copies: 1 / Formula: NAD
Molecular weightTheoretical: 663.425 Da
Chemical component information

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM / Nicotinamide adenine dinucleotide

+
Macromolecule #46: SPERMINE

MacromoleculeName: SPERMINE / type: ligand / ID: 46 / Number of copies: 1 / Formula: SPM
Molecular weightTheoretical: 202.34 Da
Chemical component information

ChemComp-SPM:
SPERMINE / Spermine

+
Macromolecule #47: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 47 / Number of copies: 23 / Formula: K
Molecular weightTheoretical: 39.098 Da

+
Macromolecule #48: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 48 / Number of copies: 2 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
Component:
ConcentrationFormulaName
20.0 mMHEPES-KOH4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid-KOH
15.0 mMKClPotassium chloride
15.0 mMMg(C2H3O2)2Magnesium acetate
0.05 %DDMn-Dodecyl B-D-maltoside
0.0075 %Cardiolipin1,3-bis(sn-3-phosphatidyl)-sn-glycerol
2.0 mMDTTDithiothreitol
GridModel: Quantifoil R2/2 / Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2.7 nm
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Calibrated magnification: 130000 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Specialist opticsEnergy filter - Name: GIF Quantum LS / Energy filter - Slit width: 20 eV
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Frames/image: 1-20 / Number real images: 3172 / Average electron dose: 35.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: Gctf
Startup modelType of model: EMDB MAP
EMDB ID:

3551

Initial angle assignmentType: PROJECTION MATCHING / Software - Name: RELION
Final 3D classificationSoftware - Name: RELION
Final angle assignmentType: PROJECTION MATCHING / Software - Name: RELION
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.03 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 24142
FSC plot (resolution estimation)

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