3-hydroxyisobutyryl-CoA hydrolase / 3-hydroxyisobutyryl-CoA hydrolase activity / mitochondrial translational initiation / L-valine catabolic process / DNA strand exchange activity / mitochondrial genome maintenance / ribonuclease III activity / ATPase inhibitor activity / mitochondrial large ribosomal subunit / mitochondrial small ribosomal subunit ...3-hydroxyisobutyryl-CoA hydrolase / 3-hydroxyisobutyryl-CoA hydrolase activity / mitochondrial translational initiation / L-valine catabolic process / DNA strand exchange activity / mitochondrial genome maintenance / ribonuclease III activity / ATPase inhibitor activity / mitochondrial large ribosomal subunit / mitochondrial small ribosomal subunit / mitochondrial translation / superoxide dismutase activity / positive regulation of proteolysis / RNA processing / peroxisome / large ribosomal subunit / single-stranded DNA binding / double-stranded RNA binding / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / mitochondrion / RNA binding / metal ion binding / cytoplasm 類似検索 - 分子機能
54S ribosomal protein L44, mitochondrial / : / Large ribosomal subunit protein uL24m / Ribosomal protein L31, mitochondrial / Ribosomal protein L20, mitochondrial / Mitochondrial homologous recombination protein 1 / 54S ribosomal protein L25 / 54S ribosomal protein L15, mitochondrial / MRPL25 domain / 54S ribosomal protein L28, mitochondrial ...54S ribosomal protein L44, mitochondrial / : / Large ribosomal subunit protein uL24m / Ribosomal protein L31, mitochondrial / Ribosomal protein L20, mitochondrial / Mitochondrial homologous recombination protein 1 / 54S ribosomal protein L25 / 54S ribosomal protein L15, mitochondrial / MRPL25 domain / 54S ribosomal protein L28, mitochondrial / 54S ribosomal protein L3, double-stranded RNA binding domain / Mitochondrial ribosomal protein subunit L20 / Transcriptional regulation of mitochondrial recombination / Mitochondrial ribosomal protein mL59 / Mitochondrial ribosomal protein L31 / 54S ribosomal protein L36, yeast / : / : / : / Ribosomal protein L31p, N-terminal / Mitochondrial ribosomal protein MRP51, fungi / Mitochondrial ribosomal protein S25 / Ribosomal protein S24, mitochondrial / Ribosomal protein S23, mitochondrial, fungi / Mitochondrial ribosomal protein subunit / Mitochondrial ribosomal protein S25 / Ribosomal protein S35, mitochondrial / Ribosomal protein MRP10, mitochondrial / Eukaryotic mitochondrial regulator protein / Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type / Small ribosomal subunit protein mS41 SAM domain / Enoyl-CoA hydratase/isomerase domain / Enoyl-CoA hydratase/isomerase / Protein Fyv4 / IGR protein motif / IGR / Ribonuclease-III-like / Mitochondrial ATPase inhibitor / Mitochondrial ATPase inhibitor, IATP / 28S ribosomal protein S25, mitochondrial / Ribonuclease III family domain profile. / Ribonuclease III family / Ribonuclease III domain / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Ribosomal protein L53, mitochondrial / 39S ribosomal protein L53/MRP-L53 / Ribosomal protein L37, mitochondrial / Mitochondrial ribosomal protein L37 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Enoyl-CoA hydratase/isomerase, conserved site / Enoyl-CoA hydratase/isomerase signature. / : / Mitochondrial ribosomal protein L46 NUDIX / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Ribosomal protein L46, N-terminal / 39S mitochondrial ribosomal protein L46 / Ribosomal protein L50, mitochondria / Ribosomal subunit 39S / Ribosomal protein L49/IMG2 / Mitochondrial large subunit ribosomal protein (Img2) / Mitochondrial mRNA-processing protein COX24, C-terminal / Ribosomal protein L27/L41, mitochondrial / : / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial ribosomal protein L27 / Mitochondrial domain of unknown function (DUF1713) / 39S ribosomal protein L46, mitochondrial / 39S ribosomal protein L43/54S ribosomal protein L51 / Ribosomal protein L47, mitochondrial / Manganese/iron superoxide dismutase, C-terminal / Manganese/iron superoxide dismutase, C-terminal domain superfamily / Manganese/iron superoxide dismutase, N-terminal domain superfamily / MRP-L47 superfamily, mitochondrial / Iron/manganese superoxide dismutases, C-terminal domain / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / Phosphatidylethanolamine-binding protein, eukaryotic / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein / PEBP-like superfamily / Double-stranded RNA binding motif / Double-stranded RNA binding motif / Ribonuclease III, endonuclease domain superfamily / Double-stranded RNA-binding domain / NUDIX domain / : / NUDIX hydrolase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / ClpP/crotonase-like domain superfamily / Ribosomal protein L1, 3-layer alpha/beta-sandwich / NUDIX hydrolase-like domain superfamily / Ribosomal protein L11, bacterial-type / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L1p/L10e family 類似検索 - ドメイン・相同性
ADENOSINE-5'-TRIPHOSPHATE / : / NICOTINAMIDE-ADENINE-DINUCLEOTIDE / SPERMINE / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Uncharacterized protein ...ADENOSINE-5'-TRIPHOSPHATE / : / NICOTINAMIDE-ADENINE-DINUCLEOTIDE / SPERMINE / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Uncharacterized protein / Small ribosomal subunit protein bS1m / Large ribosomal subunit protein uL16m / Small ribosomal subunit protein bS16m / Small ribosomal subunit protein uS3m / Large ribosomal subunit protein bL32m / Small ribosomal subunit protein uS5m / Small ribosomal subunit protein uS15m / Small ribosomal subunit protein mS33 / Small ribosomal subunit protein mS35 / Large ribosomal subunit protein uL1m / Large ribosomal subunit protein uL5m / Small ribosomal subunit protein mS41 / Large ribosomal subunit protein mL58 / Large ribosomal subunit protein bL27m / Small ribosomal subunit protein mS47 / Large ribosomal subunit protein bL31m / Large ribosomal subunit protein bL34m / Large ribosomal subunit protein bL17m / Small ribosomal subunit protein bS18m / Large ribosomal subunit protein uL3m / Small ribosomal subunit protein uS19m / Large ribosomal subunit protein uL30m / Large ribosomal subunit protein mL61 / Large ribosomal subunit protein uL11m / Large ribosomal subunit protein uL24m / Large ribosomal subunit protein mL38 / Small ribosomal subunit protein uS10m / Large ribosomal subunit protein mL67 / Small ribosomal subunit protein mS27 / Large ribosomal subunit protein bL19m / Large ribosomal subunit protein uL10m / Large ribosomal subunit protein uL6m / Large ribosomal subunit protein bL9m / Large ribosomal subunit protein mL57 / Large ribosomal subunit protein mL46 / Small ribosomal subunit protein uS13m / Small ribosomal subunit protein mS42 / Large ribosomal subunit protein mL43 / Small ribosomal subunit protein uS17m / Large ribosomal subunit protein bL36m / Small ribosomal subunit protein mS37 / Large ribosomal subunit protein mL49 / Large ribosomal subunit protein uL22m / Large ribosomal subunit protein mL41 / Small ribosomal subunit protein uS7m / Large ribosomal subunit protein mL50 / Small ribosomal subunit protein uS9m / Large ribosomal subunit protein uL29m / Small ribosomal subunit protein uS12m / Large ribosomal subunit protein uL23m / Large ribosomal subunit protein mL44 / Small ribosomal subunit protein uS4m / Small ribosomal subunit protein bS21m / Small ribosomal subunit protein bS6m / Large ribosomal subunit protein uL15m / Large ribosomal subunit protein uL14m / Large ribosomal subunit protein uL13m / Large ribosomal subunit protein bL28m / Large ribosomal subunit protein uL2m / Large ribosomal subunit protein mL54 / Small ribosomal subunit protein mS29 / Large ribosomal subunit protein mL59 / Small ribosomal subunit protein uS14m / Small ribosomal subunit protein mS46 / Large ribosomal subunit protein bL21m / Large ribosomal subunit protein mL53 / Small ribosomal subunit protein uS11m / Small ribosomal subunit protein mS45 / Small ribosomal subunit protein uS8m / Small ribosomal subunit protein mS38 / Small ribosomal subunit protein mS26 / Small ribosomal subunit protein mS23 / Large ribosomal subunit protein mL60 / Small ribosomal subunit protein uS2m / Large ribosomal subunit protein bL33m / Large ribosomal subunit protein uL4m / F(1)-ATPase inhibitor IF(1), mitochondrial 類似検索 - 構成要素
ジャーナル: Nat Commun / 年: 2020 タイトル: Analysis of translating mitoribosome reveals functional characteristics of translation in mitochondria of fungi. 著者: Yuzuru Itoh / Andreas Naschberger / Narges Mortezaei / Johannes M Herrmann / Alexey Amunts / 要旨: Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each ...Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each other is not understood. We report models of the translating fungal mitoribosome with mRNA, tRNA and nascent polypeptide, as well as an assembly intermediate. Nicotinamide adenine dinucleotide (NAD) is found in the central protuberance of the large subunit, and the ATPase inhibitory factor 1 (IF) in the small subunit. The models of the active mitoribosome explain how mRNA binds through a dedicated protein platform on the small subunit, tRNA is translocated with the help of the protein mL108, bridging it with L1 stalk on the large subunit, and nascent polypeptide paths through a newly shaped exit tunnel involving a series of structural rearrangements. An assembly intermediate is modeled with the maturation factor Atp25, providing insight into the biogenesis of the mitoribosomal large subunit and translation regulation.