Parkin-FBXW7-Cul1 ubiquitin ligase complex / F-box domain binding / PcG protein complex / regulation of epidermal cell differentiation / cullin-RING ubiquitin ligase complex / Cul7-RING ubiquitin ligase complex / positive regulation of ubiquitin protein ligase activity / Loss of Function of FBXW7 in Cancer and NOTCH1 Signaling / maintenance of protein location in nucleus / regulation of smoothened signaling pathway ...Parkin-FBXW7-Cul1 ubiquitin ligase complex / F-box domain binding / PcG protein complex / regulation of epidermal cell differentiation / cullin-RING ubiquitin ligase complex / Cul7-RING ubiquitin ligase complex / positive regulation of ubiquitin protein ligase activity / Loss of Function of FBXW7 in Cancer and NOTCH1 Signaling / maintenance of protein location in nucleus / regulation of smoothened signaling pathway / neural crest cell differentiation / negative regulation of response to oxidative stress / Nuclear events mediated by NFE2L2 / SCF ubiquitin ligase complex / ubiquitin ligase complex scaffold activity / SCF-dependent proteasomal ubiquitin-dependent protein catabolic process / Cul3-RING ubiquitin ligase complex / protein quality control for misfolded or incompletely synthesized proteins / Prolactin receptor signaling / entrainment of circadian clock by photoperiod / protein monoubiquitination / cullin family protein binding / centriolar satellite / ubiquitin-like ligase-substrate adaptor activity / cellular response to interleukin-4 / protein K48-linked ubiquitination / Nuclear events stimulated by ALK signaling in cancer / inclusion body / Regulation of BACH1 activity / intrinsic apoptotic signaling pathway / MAP3K8 (TPL2)-dependent MAPK1/3 activation / regulation of autophagy / molecular function activator activity / SCF-beta-TrCP mediated degradation of Emi1 / NIK-->noncanonical NF-kB signaling / Vpu mediated degradation of CD4 / actin filament / Dectin-1 mediated noncanonical NF-kB signaling / Degradation of GLI1 by the proteasome / animal organ morphogenesis / Activation of NF-kappaB in B cells / Negative regulation of NOTCH4 signaling / Iron uptake and transport / GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 / Degradation of GLI2 by the proteasome / GLI3 is processed to GLI3R by the proteasome / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / Degradation of beta-catenin by the destruction complex / negative regulation of DNA-binding transcription factor activity / NOTCH1 Intracellular Domain Regulates Transcription / CLEC7A (Dectin-1) signaling / SCF(Skp2)-mediated degradation of p27/p21 / Constitutive Signaling by NOTCH1 PEST Domain Mutants / Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants / FCERI mediated NF-kB activation / beta-catenin binding / Interleukin-1 signaling / Orc1 removal from chromatin / protein polyubiquitination / G1/S transition of mitotic cell cycle / Regulation of RUNX2 expression and activity / Cyclin D associated events in G1 / disordered domain specific binding / Regulation of PLK1 Activity at G2/M Transition / KEAP1-NFE2L2 pathway / Circadian Clock / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / Antigen processing: Ubiquitination & Proteasome degradation / Downstream TCR signaling / nervous system development / Neddylation / protein-macromolecule adaptor activity / cellular response to oxidative stress / midbody / ubiquitin-dependent protein catabolic process / proteasome-mediated ubiquitin-dependent protein catabolic process / positive regulation of canonical NF-kappaB signal transduction / in utero embryonic development / RNA polymerase II-specific DNA-binding transcription factor binding / cell population proliferation / Potential therapeutics for SARS / Ub-specific processing proteases / protein ubiquitination / chromatin remodeling / protein domain specific binding / centrosome / ubiquitin protein ligase binding / endoplasmic reticulum / nucleoplasm / identical protein binding / nucleus / plasma membrane / cytosol / cytoplasm 類似検索 - 分子機能
Leucine-rich repeat, cysteine-containing subtype / Leucine-rich repeat - CC (cysteine-containing) subfamily / A Receptor for Ubiquitination Targets / F-box domain profile. / F-box-like domain superfamily / F-box-like / SKP1 component, dimerisation / S-phase kinase-associated protein 1 / SKP1-like, dimerisation domain superfamily / Skp1 family, dimerisation domain ...Leucine-rich repeat, cysteine-containing subtype / Leucine-rich repeat - CC (cysteine-containing) subfamily / A Receptor for Ubiquitination Targets / F-box domain profile. / F-box-like domain superfamily / F-box-like / SKP1 component, dimerisation / S-phase kinase-associated protein 1 / SKP1-like, dimerisation domain superfamily / Skp1 family, dimerisation domain / Kelch-like ECH-associated protein 1 / : / F-box domain / BTB-kelch protein / BTB/Kelch-associated / BTB And C-terminal Kelch / Cullin protein neddylation domain / BTB And C-terminal Kelch / Leucine Rich repeat / Cullin, conserved site / Cullin family signature. / Cullin / Cullin repeat-like-containing domain superfamily / Cullin protein, neddylation domain / Cullin protein neddylation domain / Kelch / Kelch repeat type 1 / Kelch motif / Cullin / Cullin, N-terminal / Cullin homology domain / Cullin homology domain superfamily / Cullin family / Cullin family profile. / Kelch-type beta propeller / S-phase kinase-associated protein 1-like / SKP1 component, POZ domain / Skp1 family, tetramerisation domain / Found in Skp1 protein family / BTB/POZ domain / BTB domain profile. / Broad-Complex, Tramtrack and Bric a brac / BTB/POZ domain / SKP1/BTB/POZ domain superfamily / Leucine-rich repeat / Leucine-rich repeat domain superfamily / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily 類似検索 - ドメイン・相同性
S-phase kinase-associated protein 1 / Cullin-1 / Kelch-like ECH-associated protein 1 / F-box/LRR-repeat protein 17 類似検索 - 構成要素
ジャーナル: Nature / 年: 2020 タイトル: Structural basis for dimerization quality control. 著者: Elijah L Mena / Predrag Jevtić / Basil J Greber / Christine L Gee / Brandon G Lew / David Akopian / Eva Nogales / John Kuriyan / Michael Rape / 要旨: Most quality control pathways target misfolded proteins to prevent toxic aggregation and neurodegeneration. Dimerization quality control further improves proteostasis by eliminating complexes of ...Most quality control pathways target misfolded proteins to prevent toxic aggregation and neurodegeneration. Dimerization quality control further improves proteostasis by eliminating complexes of aberrant composition, but how it detects incorrect subunits remains unknown. Here we provide structural insight into target selection by SCF-FBXL17, a dimerization-quality-control E3 ligase that ubiquitylates and helps to degrade inactive heterodimers of BTB proteins while sparing functional homodimers. We find that SCF-FBXL17 disrupts aberrant BTB dimers that fail to stabilize an intermolecular β-sheet around a highly divergent β-strand of the BTB domain. Complex dissociation allows SCF-FBXL17 to wrap around a single BTB domain, resulting in robust ubiquitylation. SCF-FBXL17 therefore probes both shape and complementarity of BTB domains, a mechanism that is well suited to establish quality control of complex composition for recurrent interaction modules.
S-phasekinase-associatedprotein1 / SKP1 / Cyclin-A/CDK2-associated protein p19 / p19A / Organ of Corti protein 2 / OCP-2 / Organ of ...SKP1 / Cyclin-A/CDK2-associated protein p19 / p19A / Organ of Corti protein 2 / OCP-2 / Organ of Corti protein II / OCP-II / RNA polymerase II elongation factor-like protein / SIII / Transcription elongation factor B polypeptide 1-like / p19skp1
加速電圧: 200 kV / アライメント法: COMA FREE / C2レンズ絞り径: 50 µm / 凍結剤: NITROGEN / 電子線源: FIELD EMISSION GUN / 照射モード: FLOOD BEAM / モデル: FEI TALOS ARCTICA / モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス(公称値): 3000 nm / 試料ホルダーモデル: OTHER
ID
最小 デフォーカス(公称値) (nm)
Specimen-ID
1
2000
1
2
1500
2
撮影
ID
Imaging-ID
電子線照射量 (e/Å2)
フィルム・検出器のモデル
撮影したグリッド数
検出モード
1
1
60
GATAN K3 (6k x 4k)
1
2
2
50
GATAN K2 SUMMIT (4k x 4k)
1
SUPER-RESOLUTION
-
解析
ソフトウェア
名称: PHENIX / バージョン: 1.16_3549: / 分類: 精密化
EMソフトウェア
ID
名称
バージョン
カテゴリ
Imaging-ID
詳細
2
SerialEM
画像取得
1
4
CTFFIND
4
CTF補正
5
Gctf
CTF補正
8
UCSF Chimera
モデルフィッティング
FITINMAP
10
SerialEM
画像取得
2
11
RELION
3
初期オイラー角割当
12
cryoSPARC
初期オイラー角割当
13
RELION
3
最終オイラー角割当
15
RELION
3
3次元再構成
16
PHENIX
モデル精密化
REALSPACEREFINE
画像処理
詳細: Datasets from K3 and K2 were joined for the final reconstruction.
CTF補正
詳細: As implemented in RELION. / タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION
粒子像の選択
選択した粒子像数: 824561 詳細: Total number of particles for both datasets. Selected using RELION auto-picking (Laplacian-of-Gaussian).
対称性
点対称性: C1 (非対称)
3次元再構成
解像度: 8.5 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 160256 / アルゴリズム: FOURIER SPACE / クラス平均像の数: 2 / 対称性のタイプ: POINT
原子モデル構築
プロトコル: OTHER / 空間: REAL 詳細: Coordinates were fitted as rigid bodies or fragments in Chimera and subsequently geometry-optimized using PHENIX real space refinement.