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- PDB-6qb5: Crystal structure of the N-terminal region of human cohesin subun... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6qb5 | ||||||
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Title | Crystal structure of the N-terminal region of human cohesin subunit STAG1 | ||||||
![]() | Cohesin subunit SA-1 | ||||||
![]() | GENE REGULATION / Cohesin / SA-1 / chromosome segregation | ||||||
Function / homology | ![]() Cohesin Loading onto Chromatin / Establishment of Sister Chromatid Cohesion / mitotic cohesin complex / cohesin complex / establishment of mitotic sister chromatid cohesion / sister chromatid cohesion / mitotic spindle pole / chromosome, centromeric region / mitotic spindle assembly / localization ...Cohesin Loading onto Chromatin / Establishment of Sister Chromatid Cohesion / mitotic cohesin complex / cohesin complex / establishment of mitotic sister chromatid cohesion / sister chromatid cohesion / mitotic spindle pole / chromosome, centromeric region / mitotic spindle assembly / localization / SUMOylation of DNA damage response and repair proteins / Resolution of Sister Chromatid Cohesion / Meiotic synapsis / nuclear matrix / Separation of Sister Chromatids / chromosome / Estrogen-dependent gene expression / nuclear body / cell division / chromatin binding / chromatin / nucleoplasm / nucleus / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Newman, J.A. / Katis, V.L. / von Delft, F. / Arrowsmith, C.H. / Edwards, A. / Bountra, C. / Gileadi, O. | ||||||
Funding support | 1items
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![]() | ![]() Title: STAG1 vulnerabilities for exploiting cohesin synthetic lethality in STAG2-deficient cancers. Authors: van der Lelij, P. / Newman, J.A. / Lieb, S. / Jude, J. / Katis, V. / Hoffmann, T. / Hinterndorfer, M. / Bader, G. / Kraut, N. / Pearson, M.A. / Peters, J.M. / Zuber, J. / Gileadi, O. / Petronczki, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 270.4 KB | Display | ![]() |
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PDB format | ![]() | 215.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 450.4 KB | Display | ![]() |
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Full document | ![]() | 453.9 KB | Display | |
Data in XML | ![]() | 47.4 KB | Display | |
Data in CIF | ![]() | 67.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6r7oC ![]() 6rrcC ![]() 6rrkC ![]() 4pk7S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 39629.531 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-NA / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3 Å3/Da / Density % sol: 58.99 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 0.1 M Na/K Phosphate pH 6.0, 0.2 M NaCl, 34% PEG200 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: May 27, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9159 Å / Relative weight: 1 |
Reflection | Resolution: 2.02→110 Å / Num. obs: 125560 / % possible obs: 99.8 % / Redundancy: 6 % / CC1/2: 0.998 / Rmerge(I) obs: 0.081 / Rpim(I) all: 0.052 / Net I/σ(I): 9.8 |
Reflection shell | Resolution: 2.02→2.07 Å / Redundancy: 4.4 % / Rmerge(I) obs: 0.969 / Mean I/σ(I) obs: 1.1 / Num. unique obs: 9012 / CC1/2: 0.664 / Rpim(I) all: 0.583 / % possible all: 98 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4pk7 Resolution: 2.02→85.002 Å / SU ML: 0.26 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 28.54
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.02→85.002 Å
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Refine LS restraints |
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LS refinement shell |
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