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Yorodumi- PDB-6rnn: P46, an immunodominant surface protein from Mycoplasma hyopneumoniae -
+Open data
-Basic information
Entry | Database: PDB / ID: 6rnn | |||||||||
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Title | P46, an immunodominant surface protein from Mycoplasma hyopneumoniae | |||||||||
Components |
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Keywords | IMMUNE SYSTEM / immunodominant surface protein Mycoplasma hyopneumoniae P46 Monoclonal Antidody | |||||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta Function and homology information | |||||||||
Biological species | Mus musculus (house mouse) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.95 Å | |||||||||
Authors | Guasch, A. / Gonzalez-Gonzalez, L. / Fita, I. | |||||||||
Funding support | Spain, 2items
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Citation | Journal: Acta Crystallogr D Struct Biol / Year: 2020 Title: Structure of P46, an immunodominant surface protein from Mycoplasma hyopneumoniae: interaction with a monoclonal antibody. Authors: Guasch, A. / Montane, J. / Moros, A. / Pinol, J. / Sitja, M. / Gonzalez-Gonzalez, L. / Fita, I. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6rnn.cif.gz | 197.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6rnn.ent.gz | 149.2 KB | Display | PDB format |
PDBx/mmJSON format | 6rnn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rn/6rnn ftp://data.pdbj.org/pub/pdb/validation_reports/rn/6rnn | HTTPS FTP |
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-Related structure data
Related structure data | 6rqgC 6ruxC 6s3tC 1a3rS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Antibody | Mass: 26370.238 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Production host: Homo sapiens (human) #2: Antibody | Mass: 52172.977 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Production host: Homo sapiens (human) #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 4 / Details: 0.1 M Sodium Citrate pH 4.0 21% PEG6000 |
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-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ALBA / Beamline: XALOC / Wavelength: 1 Å |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: May 17, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.95→61 Å / Num. obs: 94930 / % possible obs: 94.02 % / Redundancy: 18.4 % / CC1/2: 0.98 / Net I/σ(I): 5.92 |
Reflection shell | Resolution: 1.95→2 Å / Num. unique obs: 5588 / CC1/2: 0.27 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1A3R Resolution: 1.95→61 Å / Cross valid method: FREE R-VALUE
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Displacement parameters | Biso mean: 31.1 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.95→61 Å
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Refine LS restraints |
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