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- PDB-6s3t: P46, an immunodominant surface protein from Mycoplasma hyopneumoniae -
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Open data
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Basic information
Entry | Database: PDB / ID: 6s3t | ||||||||||||
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Title | P46, an immunodominant surface protein from Mycoplasma hyopneumoniae | ||||||||||||
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![]() | PROTEIN BINDING / immunodominant surface protein Mycoplasma hyopneumoniae P46 Complex with monolonal antibody | ||||||||||||
Function / homology | ![]() outer membrane-bounded periplasmic space / carbohydrate binding / plasma membrane Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Guasch, A. / Gonzalez-Gonzalez, L. / Fita, I. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure of P46, an immunodominant surface protein from Mycoplasma hyopneumoniae: interaction with a monoclonal antibody. Authors: Guasch, A. / Montane, J. / Moros, A. / Pinol, J. / Sitja, M. / Gonzalez-Gonzalez, L. / Fita, I. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 769.6 KB | Display | ![]() |
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PDB format | ![]() | 504.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.7 MB | Display | ![]() |
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Full document | ![]() | 1.7 MB | Display | |
Data in XML | ![]() | 106.5 KB | Display | |
Data in CIF | ![]() | 144.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6rnnSC ![]() 6rqgSC ![]() 6ruxC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 42426.508 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() References: UniProt: P0C0J8 #2: Antibody | Mass: 26370.238 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Antibody | Mass: 52172.977 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #4: Sugar | ChemComp-XYP / #5: Chemical | ChemComp-NA / Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.53 Å3/Da / Density % sol: 65.18 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / Details: 0.1M MIB pH 4.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: May 17, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 3.5→29.3 Å / Num. obs: 83357 / % possible obs: 98.8 % / Redundancy: 1.9 % / CC1/2: 0.981 / Net I/σ(I): 5.51 |
Reflection shell | Resolution: 3.5→3.6 Å / Num. unique obs: 8278 / CC1/2: 0.292 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6RQG, 6RNN Resolution: 3.5→29 Å / Cross valid method: FREE R-VALUE / σ(F): 2.4 / Phase error: 31.8932
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||
Displacement parameters | Biso mean: 103.39 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.5→29 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.5→29.3 Å
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