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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 6kea | ||||||
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タイトル | crystal structure of MBP-tagged REV7-IpaB complex | ||||||
![]() | Maltose-binding periplasmic protein,LINKER,hREV7,LINKER,Invasin IpaB,hREV3 | ||||||
![]() | CELL CYCLE / REV7 / MAD2B / MAD2L2 / Shigella Invasin IpaB | ||||||
機能・相同性 | ![]() somatic diversification of immunoglobulins involved in immune response / DNA damage response, signal transduction resulting in transcription / telomere maintenance in response to DNA damage / negative regulation of transcription regulatory region DNA binding / zeta DNA polymerase complex / positive regulation of isotype switching / positive regulation of extracellular matrix assembly / negative regulation of transcription by competitive promoter binding / negative regulation of cell-cell adhesion mediated by cadherin / JUN kinase binding ...somatic diversification of immunoglobulins involved in immune response / DNA damage response, signal transduction resulting in transcription / telomere maintenance in response to DNA damage / negative regulation of transcription regulatory region DNA binding / zeta DNA polymerase complex / positive regulation of isotype switching / positive regulation of extracellular matrix assembly / negative regulation of transcription by competitive promoter binding / negative regulation of cell-cell adhesion mediated by cadherin / JUN kinase binding / negative regulation of epithelial to mesenchymal transition / negative regulation of ubiquitin protein ligase activity / detection of maltose stimulus / maltose transport complex / mitotic spindle assembly checkpoint signaling / positive regulation of double-strand break repair via nonhomologous end joining / carbohydrate transport / carbohydrate transmembrane transporter activity / maltose binding / maltose transport / host cell membrane / maltodextrin transmembrane transport / negative regulation of double-strand break repair via homologous recombination / error-prone translesion synthesis / positive regulation of epithelial to mesenchymal transition / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / ATP-binding cassette (ABC) transporter complex / Translesion synthesis by REV1 / Translesion synthesis by POLK / Translesion synthesis by POLI / cell chemotaxis / regulation of cell growth / actin filament organization / double-strand break repair via homologous recombination / negative regulation of canonical Wnt signaling pathway / negative regulation of protein catabolic process / negative regulation of DNA-binding transcription factor activity / DNA-templated DNA replication / spindle / host cell / double-strand break repair / positive regulation of peptidyl-serine phosphorylation / chromosome / site of double-strand break / 4 iron, 4 sulfur cluster binding / outer membrane-bounded periplasmic space / RNA polymerase II-specific DNA-binding transcription factor binding / periplasmic space / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / cell division / nucleotide binding / DNA damage response / host cell nucleus / chromatin / nucleolus / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / DNA binding / extracellular region / nucleoplasm / membrane / nucleus / metal ion binding / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() synthetic construct (人工物) ![]() ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Wang, X. / Pernicone, N. / Pertz, L. / Hua, D.P. / Zhang, T.Q. / Listovsky, T. / Xie, W. | ||||||
資金援助 | ![]()
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![]() | ![]() タイトル: REV7 has a dynamic adaptor region to accommodate small GTPase RAN/ShigellaIpaB ligands, and its activity is regulated by the RanGTP/GDP switch. 著者: Wang, X. / Pernicone, N. / Pertz, L. / Hua, D. / Zhang, T. / Listovsky, T. / Xie, W. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 447.9 KB | 表示 | ![]() |
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PDB形式 | ![]() | 369 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 464.8 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 489.5 KB | 表示 | |
XML形式データ | ![]() | 78.3 KB | 表示 | |
CIF形式データ | ![]() | 108.5 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 68963.570 Da / 分子数: 4 / 変異: D83A/K84A/E173A/N174A/K240A/R485A / 由来タイプ: 組換発現 詳細: MBP-tagged human REV7 and Shigella Invasin IpaB fusion protein 由来: (組換発現) ![]() ![]() ![]() ![]() 株: K12 / 発現宿主: ![]() ![]() 参照: UniProt: P0AEX9, UniProt: Q9UI95, UniProt: P18011, UniProt: O60673 #2: 水 | ChemComp-HOH / | 配列の詳細 | residues 360-373 and 573-596 are LINKER | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.41 Å3/Da / 溶媒含有率: 48.9 % |
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結晶化 | 温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 5.6 / 詳細: 100 mM Bistris, pH 5.6, 25% PEG3350 |
-データ収集
回折 | 平均測定温度: 100 K / Serial crystal experiment: N |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: PSI PILATUS 6M / 検出器: PIXEL / 日付: 2016年11月20日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.9792 Å / 相対比: 1 |
反射 | 解像度: 2.35→29.91 Å / Num. obs: 98435 / % possible obs: 97.5 % / 冗長度: 7.2 % / Rmerge(I) obs: 0.1366 / Net I/σ(I): 14.22 |
反射 シェル | 解像度: 2.35→2.43 Å / 冗長度: 7.3 % / Rmerge(I) obs: 0.8728 / % possible all: 97.8 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: 3VD8, 3ABD 解像度: 2.35→29.91 Å / 交差検証法: FREE R-VALUE
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精密化ステップ | サイクル: LAST / 解像度: 2.35→29.91 Å
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LS精密化 シェル | 解像度: 2.35→2.434 Å
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