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Open data
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Basic information
Entry | Database: PDB / ID: 1atn | |||||||||
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Title | Atomic structure of the actin:DNASE I complex | |||||||||
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![]() | ENDODEOXYRIBONUCLEASE | |||||||||
Function / homology | ![]() Striated Muscle Contraction / regulation of neutrophil mediated cytotoxicity / zymogen granule / regulation of acute inflammatory response / dynactin complex / deoxyribonuclease I / neutrophil activation involved in immune response / deoxyribonuclease I activity / DNA catabolic process / cytoskeletal motor activator activity ...Striated Muscle Contraction / regulation of neutrophil mediated cytotoxicity / zymogen granule / regulation of acute inflammatory response / dynactin complex / deoxyribonuclease I / neutrophil activation involved in immune response / deoxyribonuclease I activity / DNA catabolic process / cytoskeletal motor activator activity / tropomyosin binding / mesenchyme migration / troponin I binding / myosin heavy chain binding / filamentous actin / actin filament bundle / skeletal muscle thin filament assembly / striated muscle thin filament / actin filament bundle assembly / skeletal muscle myofibril / actin monomer binding / skeletal muscle fiber development / stress fiber / titin binding / actin filament polymerization / filopodium / actin filament / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / calcium-dependent protein binding / lamellipodium / nuclear envelope / actin binding / cell body / hydrolase activity / protein domain specific binding / apoptotic process / calcium ion binding / positive regulation of gene expression / magnesium ion binding / DNA binding / extracellular region / ATP binding / identical protein binding / nucleus / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() | |||||||||
![]() | Kabsch, W. / Mannherz, H.G. / Suck, D. / Pai, E. / Holmes, K.C. | |||||||||
![]() | ![]() Title: Atomic structure of the actin:DNase I complex. Authors: Kabsch, W. / Mannherz, H.G. / Suck, D. / Pai, E.F. / Holmes, K.C. #1: ![]() Title: Structure and Function of Actin Authors: Kabsch, W. / Vandekerckhove, J. #2: ![]() Title: Muscle Proteins: Actin Authors: Holmes, K.C. / Kabsch, W. #3: ![]() Title: Similarity of the Three-Dimensional Structures of Actin and the ATPase Fragment of a 70-kDa Heat Shock Cognate Protein Authors: Flaherty, K.M. / Mckay, D.B. / Kabsch, W. / Holmes, K.C. #4: ![]() Title: Atomic Model of the Actin Filament Authors: Holmes, K.C. / Popp, D. / Gebhard, W. / Kabsch, W. #5: ![]() Title: Crystallographic Refinement and Structure of DNase I at 2 Angstroms Resolution Authors: Oefner, C. / Suck, D. #6: ![]() Title: Three-Dimensional Structure of the Complex of Actin and DNase I at 4.5 Angstroms Resolution Authors: Kabsch, W. / Mannherz, H.G. / Suck, D. #7: ![]() Title: Three-Dimensional Structure of Bovine Pancreatic DNase I at 2.5 Angstroms Resolution Authors: Suck, D. / Oefner, C. / Kabsch, W. #8: ![]() Title: Three-Dimensional Structure of the Complex of Skeletal Muscle Actin and Bovine Pancreatic DNase I at 6-Angstroms Resolution Authors: Suck, D. / Kabsch, W. / Mannherz, H.G. | |||||||||
History |
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Remark 700 | SHEET SHEETS A4A AND A4B ARE IDENTICAL EXCEPT FOR THE FIRST STRAND. THIS STRAND IS SPLIT INTO TWO ...SHEET SHEETS A4A AND A4B ARE IDENTICAL EXCEPT FOR THE FIRST STRAND. THIS STRAND IS SPLIT INTO TWO DISTINCT AMINO ACID RUNS. THE BULGE OF 5 AMINO ACIDS INCLUDES HELIX 7. SHEET D1B IS AN EXTENSION OF SHEET D1A. STRANDS 1 TO 4 OF D1B ARE AN EXTENSION OF STRANDS 2 TO 5 OF D1A. SHEETS D2A AND D2B ARE IDENTICAL EXCEPT FOR THE FIRST STRAND. THIS STRAND IS INTERRUPTED BY ONE BULGE RESIDUE. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 136.3 KB | Display | ![]() |
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PDB format | ![]() | 107.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 542.6 KB | Display | ![]() |
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Full document | ![]() | 564.1 KB | Display | |
Data in XML | ![]() | 16.9 KB | Display | |
Data in CIF | ![]() | 24.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUE HIS A 73 IS A METHYLATED HISTIDINE. |
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Components
#1: Protein | Mass: 41522.176 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() | ||||
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#2: Protein | Mass: 29047.539 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() | ||||
#3: Polysaccharide | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source | ||||
#4: Chemical | ChemComp-CA / #5: Chemical | ChemComp-ATP / | Nonpolymer details | THERE IS A CARBOHYDRATE MOIETY CONSISTING OF TWO N-ACETYLGLUCOSAMINE AND 5 MANNOSE RESIDUES WHICH ...THERE IS A CARBOHYDRA | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.91 Å3/Da / Density % sol: 57.69 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 4 ℃ / pH: 6.6 / Method: unknown | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.4 Å / Num. all: 32996 / Num. obs: 31362 / % possible obs: 81 % / Observed criterion σ(I): 1 / Num. measured all: 103367 / Rmerge(I) obs: 0.107 |
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Processing
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Refinement | Resolution: 2.8→10 Å / Rfactor Rwork: 0.21 / σ(I): 1 | ||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→10 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 10 Å / Rfactor obs: 0.21 | ||||||||||||
Solvent computation | *PLUS | ||||||||||||
Displacement parameters | *PLUS | ||||||||||||
Refine LS restraints | *PLUS
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