温度: 277 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 6.6 詳細: 0.1 M MOPSO (pH 6.6), 0.3 M Mg(NO3)2, 19% (v/v) PEG 4000, and 5 mM DTT. H2O2 soaking was performed by adding hydrogen peroxide to 1 mM to the cryoprotection solution (17% glycerol and 83% ...詳細: 0.1 M MOPSO (pH 6.6), 0.3 M Mg(NO3)2, 19% (v/v) PEG 4000, and 5 mM DTT. H2O2 soaking was performed by adding hydrogen peroxide to 1 mM to the cryoprotection solution (17% glycerol and 83% reservoir solution) and incubating at 277 K for 75 min