+Open data
-Basic information
Entry | Database: PDB / ID: 6bwy | ||||||
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Title | DNA substrate selection by APOBEC3G | ||||||
Components |
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Keywords | HYDROLASE / Deaminase / DNA binding / complex | ||||||
Function / homology | Function and homology information apolipoprotein B mRNA editing enzyme complex / dCTP deaminase activity / telomere cap complex / cytidine deamination / chromosome, telomeric repeat region / telomerase inhibitor activity / base conversion or substitution editing / single-stranded DNA cytosine deaminase / DNA cytosine deamination / cytidine to uridine editing ...apolipoprotein B mRNA editing enzyme complex / dCTP deaminase activity / telomere cap complex / cytidine deamination / chromosome, telomeric repeat region / telomerase inhibitor activity / base conversion or substitution editing / single-stranded DNA cytosine deaminase / DNA cytosine deamination / cytidine to uridine editing / deoxycytidine deaminase activity / cytidine deaminase activity / shelterin complex / negative regulation of single stranded viral RNA replication via double stranded DNA intermediate / negative regulation of viral process / regulation of telomere maintenance via telomerase / retrotransposon silencing / nuclear telomere cap complex / G-rich strand telomeric DNA binding / single-stranded telomeric DNA binding / telomere capping / DNA demethylation / negative regulation of viral genome replication / APOBEC3G mediated resistance to HIV-1 infection / positive regulation of defense response to virus by host / telomere maintenance / P-body / Vif-mediated degradation of APOBEC3G / defense response to virus / ribonucleoprotein complex / innate immune response / RNA binding / zinc ion binding / identical protein binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Schizosaccharomyces pombe (fission yeast) Homo sapiens (human) synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.9 Å | ||||||
Authors | Ziegler, S.J. / Buzovetsky, O. | ||||||
Citation | Journal: PLoS ONE / Year: 2018 Title: Insights into DNA substrate selection by APOBEC3G from structural, biochemical, and functional studies. Authors: Ziegler, S.J. / Liu, C. / Landau, M. / Buzovetsky, O. / Desimmie, B.A. / Zhao, Q. / Sasaki, T. / Burdick, R.C. / Pathak, V.K. / Anderson, K.S. / Xiong, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6bwy.cif.gz | 607.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6bwy.ent.gz | 499 KB | Display | PDB format |
PDBx/mmJSON format | 6bwy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bw/6bwy ftp://data.pdbj.org/pub/pdb/validation_reports/bw/6bwy | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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4 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 0 / Beg auth comp-ID: GLN / Beg label comp-ID: GLN / End auth comp-ID: ASN / End label comp-ID: ASN / Refine code: 0 / Auth seq-ID: 30 - 381 / Label seq-ID: 7 - 358
NCS ensembles :
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-Components
#1: DNA chain | Mass: 9357.086 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #2: Protein | Mass: 41966.359 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (yeast), (gene. exp.) Homo sapiens (human) Strain: 972 / ATCC 24843 / Gene: pot1, SPAC26H5.06, APOBEC3G, MDS019 / Production host: Escherichia coli (E. coli) References: UniProt: O13988, UniProt: Q9HC16, Hydrolases; Acting on carbon-nitrogen bonds, other than peptide bonds; In cyclic amidines #3: Chemical | ChemComp-PO4 / #4: Chemical | ChemComp-ZN / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal grow | Temperature: 298 K / Method: batch mode Details: 100 mM HEPES pH7, 200 mM LiCl, and 20% (w/v) Polyethylene glycol (PEG) 6000 |
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-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X29A / Wavelength: 1.075 Å | |||||||||||||||
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 28, 2012 | |||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength | Wavelength: 1.075 Å / Relative weight: 1 | |||||||||||||||
Reflection twin |
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Reflection | Resolution: 2.9→44.19 Å / Num. obs: 35772 / % possible obs: 97.5 % / Redundancy: 3.3 % / CC1/2: 0.7 / Rmerge(I) obs: 0.105 / Rpim(I) all: 0.064 / Net I/σ(I): 5.1 | |||||||||||||||
Reflection shell | Resolution: 2.9→3 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.856 / Mean I/σ(I) obs: 1.1 / Num. unique obs: 3545 / Rpim(I) all: 0.539 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3IR2, 1QZH Resolution: 2.9→44.19 Å / Cor.coef. Fo:Fc: 0.937 / Cor.coef. Fo:Fc free: 0.923 / SU B: 27.433 / SU ML: 0.267 / Cross valid method: THROUGHOUT / ESU R Free: 0.109
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 88.213 Å2
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Refinement step | Cycle: 1 / Resolution: 2.9→44.19 Å
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Refine LS restraints |
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