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- PDB-5x3f: Crystal structure of the YgjG-Protein A-Zpa963-PKA catalytic domain -
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Basic information
Entry | Database: PDB / ID: 5x3f | ||||||
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Title | Crystal structure of the YgjG-Protein A-Zpa963-PKA catalytic domain | ||||||
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![]() | TRANSFERASE / synthetic protein / LYASE | ||||||
Function / homology | ![]() diamine transaminase / putrescine-2-oxoglutarate transaminase / diamine transaminase activity / putrescine--2-oxoglutarate transaminase activity / L-lysine catabolic process / putrescine catabolic process / spontaneous exocytosis of neurotransmitter / PKA activation in glucagon signalling / CREB1 phosphorylation through the activation of Adenylate Cyclase / negative regulation of meiotic cell cycle ...diamine transaminase / putrescine-2-oxoglutarate transaminase / diamine transaminase activity / putrescine--2-oxoglutarate transaminase activity / L-lysine catabolic process / putrescine catabolic process / spontaneous exocytosis of neurotransmitter / PKA activation in glucagon signalling / CREB1 phosphorylation through the activation of Adenylate Cyclase / negative regulation of meiotic cell cycle / HDL assembly / DARPP-32 events / Rap1 signalling / PKA activation / Vasopressin regulates renal water homeostasis via Aquaporins / Regulation of insulin secretion / GPER1 signaling / Hedgehog 'off' state / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / MAPK6/MAPK4 signaling / GLI3 is processed to GLI3R by the proteasome / AURKA Activation by TPX2 / Factors involved in megakaryocyte development and platelet production / Regulation of PLK1 Activity at G2/M Transition / Interleukin-3, Interleukin-5 and GM-CSF signaling / CD209 (DC-SIGN) signaling / Mitochondrial protein degradation / RET signaling / Ion homeostasis / VEGFA-VEGFR2 Pathway / regulation of cellular respiration / regulation of protein processing / protein localization to lipid droplet / regulation of bicellular tight junction assembly / cellular response to parathyroid hormone stimulus / IgG binding / cAMP-dependent protein kinase / cellular response to cold / regulation of osteoblast differentiation / sperm capacitation / cAMP-dependent protein kinase activity / ciliary base / negative regulation of glycolytic process through fructose-6-phosphate / cAMP-dependent protein kinase complex / AMP-activated protein kinase activity / postsynaptic modulation of chemical synaptic transmission / cellular response to glucagon stimulus / protein kinase A regulatory subunit binding / plasma membrane raft / axoneme / mesoderm formation / sperm flagellum / negative regulation of smoothened signaling pathway / regulation of proteasomal protein catabolic process / regulation of synaptic transmission, glutamatergic / positive regulation of gluconeogenesis / sperm midpiece / negative regulation of TORC1 signaling / protein kinase A signaling / protein serine/threonine/tyrosine kinase activity / protein export from nucleus / positive regulation of protein export from nucleus / acrosomal vesicle / neural tube closure / cellular response to glucose stimulus / modulation of chemical synaptic transmission / neuromuscular junction / adenylate cyclase-activating G protein-coupled receptor signaling pathway / positive regulation of insulin secretion / mRNA processing / small GTPase binding / presynapse / pyridoxal phosphate binding / cellular response to heat / manganese ion binding / postsynapse / peptidyl-serine phosphorylation / dendritic spine / regulation of cell cycle / protein kinase activity / nuclear speck / protein domain specific binding / protein serine kinase activity / protein serine/threonine kinase activity / centrosome / glutamatergic synapse / ubiquitin protein ligase binding / positive regulation of cell population proliferation / protein-containing complex binding / protein kinase binding / perinuclear region of cytoplasm / negative regulation of transcription by RNA polymerase II / magnesium ion binding / protein homodimerization activity / protein-containing complex Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Youn, S.J. / Kwon, N.Y. / Lee, J.H. / Kim, J.H. / Lee, H. / Lee, J.O. | ||||||
![]() | ![]() Title: Construction of novel repeat proteins with rigid and predictable structures using a shared helix method. Authors: Youn, S.J. / Kwon, N.Y. / Lee, J.H. / Kim, J.H. / Choi, J. / Lee, H. / Lee, J.O. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 351.5 KB | Display | ![]() |
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PDB format | ![]() | 290 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 449.5 KB | Display | ![]() |
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Full document | ![]() | 477.5 KB | Display | |
Data in XML | ![]() | 32.8 KB | Display | |
Data in CIF | ![]() | 44 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5h75C ![]() 5h76C ![]() 5h77C ![]() 5h78C ![]() 5h79C ![]() 5h7aC ![]() 5h7bC ![]() 5h7cC ![]() 5h7dC ![]() 5xbyC ![]() 2m5aS ![]() 4ntsS ![]() 4uoyS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 54451.414 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 7-453,220-269 / Mutation: N222V, G240A Source method: isolated from a genetically manipulated source Details: The fusion protein of Putrescine aminotransferase (UNP RESIDUES 7-453) and Immunoglobulin G-binding protein A (UNP RESIDUES 220-269) Source: (gene. exp.) ![]() ![]() ![]() ![]() Strain: K12 / Gene: patA, ygjG, b3073, JW5510, spa / Production host: ![]() ![]() References: UniProt: P42588, UniProt: P38507, putrescine-2-oxoglutarate transaminase |
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#2: Protein | Mass: 45479.789 Da / Num. of mol.: 1 / Fragment: residues 97-156,UNP RESIDUES 11-343 Source method: isolated from a genetically manipulated source Details: The fusion protein of Zpa963 and cAMP-dependent protein kinase catalytic subunit alpha (UNP RESIDUES 11-343) Source: (gene. exp.) ![]() ![]() ![]() ![]() Gene: Prkaca, Pkaca / Production host: ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.55 Å3/Da / Density % sol: 77.84 % |
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Crystal grow | Temperature: 296 K / Method: vapor diffusion / pH: 5.5 / Details: 91mM MES pH 5.5, 2.33M Na formate |
-Data collection
Diffraction | Mean temperature: 80 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Dec 14, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97934 Å / Relative weight: 1 |
Reflection | Resolution: 3.38→50 Å / Num. obs: 31056 / % possible obs: 99.7 % / Redundancy: 6.5 % / Net I/σ(I): 13.7 |
Reflection shell | Resolution: 3.38→3.52 Å / Redundancy: 6.1 % / % possible all: 99.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4UOY, 2M5A, 4NTS Resolution: 3.38→50 Å / Cor.coef. Fo:Fc: 0.946 / Cor.coef. Fo:Fc free: 0.939 / SU B: 42.478 / SU ML: 0.288 / Cross valid method: THROUGHOUT / ESU R Free: 0.371 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 135.845 Å2
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Refinement step | Cycle: 1 / Resolution: 3.38→50 Å
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Refine LS restraints |
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