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- PDB-5h77: Crystal structure of the PKA-protein A fusion protein -

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Basic information

Entry
Database: PDB / ID: 5h77
TitleCrystal structure of the PKA-protein A fusion protein
ComponentscAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A
KeywordsSIGNALING PROTEIN / IMMUNE SYSTEM / synthetic protein
Function / homology
Function and homology information


ROBO receptors bind AKAP5 / cAMP-dependent protein kinase regulator activity / negative regulation of cAMP-dependent protein kinase activity / nucleotide-activated protein kinase complex / IgG binding / cAMP-dependent protein kinase inhibitor activity / CREB1 phosphorylation through the activation of Adenylate Cyclase / PKA activation / ciliary base / cAMP-dependent protein kinase complex ...ROBO receptors bind AKAP5 / cAMP-dependent protein kinase regulator activity / negative regulation of cAMP-dependent protein kinase activity / nucleotide-activated protein kinase complex / IgG binding / cAMP-dependent protein kinase inhibitor activity / CREB1 phosphorylation through the activation of Adenylate Cyclase / PKA activation / ciliary base / cAMP-dependent protein kinase complex / plasma membrane raft / protein kinase A catalytic subunit binding / PKA activation in glucagon signalling / DARPP-32 events / Hedgehog 'off' state / cAMP binding / FCGR3A-mediated IL10 synthesis / Vasopressin regulates renal water homeostasis via Aquaporins / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / ADORA2B mediated anti-inflammatory cytokines production / GPER1 signaling / Factors involved in megakaryocyte development and platelet production / intracellular signal transduction / protein domain specific binding / focal adhesion / centrosome / ubiquitin protein ligase binding / protein-containing complex / extracellular exosome / extracellular region / membrane / plasma membrane / cytoplasm / cytosol
Similarity search - Function
cAMP-dependent protein kinase regulatory subunit / cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain / Regulatory subunit of type II PKA R-subunit / RIIalpha, Regulatory subunit portion of type II PKA R-subunit / Octapeptide repeat / Octapeptide repeat / Protein A, Ig-binding domain / B domain / Lysin motif / LysM domain superfamily ...cAMP-dependent protein kinase regulatory subunit / cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain / Regulatory subunit of type II PKA R-subunit / RIIalpha, Regulatory subunit portion of type II PKA R-subunit / Octapeptide repeat / Octapeptide repeat / Protein A, Ig-binding domain / B domain / Lysin motif / LysM domain superfamily / LysM domain / LysM domain profile. / LysM domain / Immunoglobulin/albumin-binding domain superfamily / YSIRK type signal peptide / Cyclic nucleotide-binding domain signature 2. / Cyclic nucleotide-binding domain signature 1. / Cyclic nucleotide-binding, conserved site / YSIRK Gram-positive signal peptide / LPXTG cell wall anchor motif / Cyclic nucleotide-monophosphate binding domain / Cyclic nucleotide-binding domain / cAMP/cGMP binding motif profile. / Cyclic nucleotide-binding domain / Gram-positive cocci surface proteins LPxTG motif profile. / Cyclic nucleotide-binding domain superfamily / LPXTG cell wall anchor domain / RmlC-like jelly roll fold
Similarity search - Domain/homology
cAMP-dependent protein kinase type II-alpha regulatory subunit / Immunoglobulin G-binding protein A
Similarity search - Component
Biological speciesHomo sapiens (human)
Staphylococcus aureus (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.197 Å
AuthorsYoun, S.J. / Kwon, N.Y. / Lee, J.H. / Kim, J.H. / Lee, H. / Lee, J.O.
CitationJournal: Sci Rep / Year: 2017
Title: Construction of novel repeat proteins with rigid and predictable structures using a shared helix method.
Authors: Youn, S.J. / Kwon, N.Y. / Lee, J.H. / Kim, J.H. / Choi, J. / Lee, H. / Lee, J.O.
History
DepositionNov 17, 2016Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jun 28, 2017Provider: repository / Type: Initial release
Revision 1.1Nov 8, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A
B: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A
C: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A
D: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A
E: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A
F: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A
G: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A
H: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A
I: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A
J: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A
K: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A
L: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A


Theoretical massNumber of molelcules
Total (without water)123,77412
Polymers123,77412
Non-polymers00
Water00
1
A: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A
B: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A


Theoretical massNumber of molelcules
Total (without water)20,6292
Polymers20,6292
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2010 Å2
ΔGint-19 kcal/mol
Surface area11050 Å2
MethodPISA
2
C: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A
D: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A


Theoretical massNumber of molelcules
Total (without water)20,6292
Polymers20,6292
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2010 Å2
ΔGint-21 kcal/mol
Surface area10970 Å2
MethodPISA
3
E: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A
F: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A


Theoretical massNumber of molelcules
Total (without water)20,6292
Polymers20,6292
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2050 Å2
ΔGint-18 kcal/mol
Surface area10830 Å2
MethodPISA
4
G: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A
H: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A


Theoretical massNumber of molelcules
Total (without water)20,6292
Polymers20,6292
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1950 Å2
ΔGint-20 kcal/mol
Surface area10780 Å2
MethodPISA
5
I: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A
J: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A


Theoretical massNumber of molelcules
Total (without water)20,6292
Polymers20,6292
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2010 Å2
ΔGint-19 kcal/mol
Surface area11180 Å2
MethodPISA
6
K: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A
L: cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A


Theoretical massNumber of molelcules
Total (without water)20,6292
Polymers20,6292
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1840 Å2
ΔGint-19 kcal/mol
Surface area11210 Å2
MethodPISA
Unit cell
Length a, b, c (Å)71.588, 135.378, 88.988
Angle α, β, γ (deg.)90.00, 108.77, 90.00
Int Tables number4
Space group name H-MP1211

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Components

#1: Antibody
cAMP-dependent protein kinase type II-alpha regulatory subunit,Immunoglobulin G-binding protein A / IgG-binding protein A / Staphylococcal protein A


Mass: 10314.522 Da / Num. of mol.: 12 / Fragment: UNP RESIDUES 5-41,UNP RESIDUES 220-268 / Mutation: G222A, G240A
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human), (gene. exp.) Staphylococcus aureus (bacteria)
Gene: PRKAR2A, PKR2, PRKAR2, spa / Production host: Escherichia coli (E. coli) / References: UniProt: P13861, UniProt: P38507

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.51 Å3/Da / Density % sol: 64.99 %
Crystal growTemperature: 296 K / Method: vapor diffusion / pH: 4.5
Details: 0.1M sodium acetate pH 4.5, 0.68M sodium citrate pH 4.5

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Data collection

DiffractionMean temperature: 80 K
Diffraction sourceSource: SYNCHROTRON / Site: PAL/PLS / Beamline: 7A (6B, 6C1) / Wavelength: 0.97934 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Mar 21, 2016
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97934 Å / Relative weight: 1
ReflectionResolution: 3.2→50 Å / Num. obs: 26459 / % possible obs: 99.4 % / Redundancy: 3.3 % / Net I/σ(I): 11.2
Reflection shellResolution: 3.2→3.31 Å / Redundancy: 3 % / Mean I/σ(I) obs: 1.8 / % possible all: 99.1

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Processing

Software
NameVersionClassification
PHENIX(1.10.1_2155)refinement
HKL-2000data reduction
SCALEPACKdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 2IZX, 2SPZ

2izx

2spz


Resolution: 3.197→42.419 Å / SU ML: 0.59 / Cross valid method: FREE R-VALUE / σ(F): 1.37 / Phase error: 32.37 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2845 2003 7.58 %
Rwork0.2389 --
obs0.2424 26430 99.24 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 3.197→42.419 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8143 0 0 0 8143
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0058351
X-RAY DIFFRACTIONf_angle_d0.9911340
X-RAY DIFFRACTIONf_dihedral_angle_d14.3265197
X-RAY DIFFRACTIONf_chiral_restr0.0531259
X-RAY DIFFRACTIONf_plane_restr0.0071534
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.197-3.27690.43471260.36331721X-RAY DIFFRACTION97
3.2769-3.36550.37231460.34271721X-RAY DIFFRACTION99
3.3655-3.46440.35121480.31351724X-RAY DIFFRACTION99
3.4644-3.57620.3951340.30341756X-RAY DIFFRACTION100
3.5762-3.7040.34991430.28461744X-RAY DIFFRACTION100
3.704-3.85220.35251530.27411745X-RAY DIFFRACTION100
3.8522-4.02740.29391360.25351746X-RAY DIFFRACTION100
4.0274-4.23950.28591460.23581757X-RAY DIFFRACTION100
4.2395-4.50480.27161500.20731743X-RAY DIFFRACTION100
4.5048-4.85220.20971400.19951733X-RAY DIFFRACTION100
4.8522-5.33970.23281370.21341754X-RAY DIFFRACTION100
5.3397-6.11040.28831550.25431752X-RAY DIFFRACTION100
6.1104-7.69090.30171440.24771768X-RAY DIFFRACTION100
7.6909-42.42320.20821450.16281763X-RAY DIFFRACTION98
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.0938-1.725-1.13723.49454.38538.4681-0.0680.78310.36030.5742-0.2504-0.2530.38250.80450.34540.40140.02020.02040.75530.36330.678368.1115-23.926420.2269
24.8982-1.95881.44631.4444-0.65310.4345-0.5337-1.90680.50320.52950.42810.50750.62250.7961-0.01711.2945-0.25160.25281.589-0.39420.777368.427-47.29616.5806
35.2285-2.8872-0.73782.49651.57915.57310.03061.70820.598-0.6069-1.0554-0.6785-0.0534-0.36080.21670.95840.40970.28241.65450.22980.622877.5135-37.66698.3004
43.8252-0.64830.53962.29131.63814.0551-0.03721.1668-0.6283-0.9843-0.554-0.29370.3710.70970.61630.92110.19170.24151.06970.0550.527868.1524-41.79910.7243
50.04820.08280.08680.14840.17640.4081-0.0621-0.10920.008-0.0698-0.07250.0528-0.14270.2012-0.04370.5272-0.16110.0210.87080.52280.794667.0271-10.4832.653
67.1055-0.0363-0.03882.2142.20362.1675-0.36850.3377-0.0365-0.6836-1.17610.4829-0.1518-1.03071.18130.37820.0677-0.09260.4493-0.11770.58858.0363-15.227914.7868
76.16774.9276-2.18834.6704-1.06431.64160.3910.37631.0371-0.8708-0.8055-0.4955-0.1651-0.3527-0.00030.49020.10830.0560.47750.32810.774372.4512-7.655711.5213
83.9563-0.57243.07942.9487-1.42747.01810.27691.31410.2542-0.73680.15-1.33591.00970.2124-0.30840.686-0.0280.09910.50350.07341.185282.1816-5.17084.8938
92.3361-2.6423-0.64053.20552.00226.3099-0.535-0.91260.67250.69050.1053-1.58250.84580.21240.26490.75530.119-0.05740.3792-0.15091.224686.8327-1.927714.8441
101.05080.6987-2.39719.236-7.17128.634-0.0231-0.1439-0.39510.8425-0.0007-0.0706-0.96460.16340.03240.4742-0.08780.06580.4135-0.05070.365855.735712.192526.5662
113.26553.093.13994.8542.25273.32140.49851.8355-0.07770.06080.2625-0.15420.04831.862-0.83490.761-0.08190.09690.947-0.01580.51358.663726.11339.4226
122.0807-1.4466-0.39633.24.82589.4738-0.40650.3604-0.7310.63380.3464-1.4228-0.40790.7320.30130.48670.04130.06360.68290.11960.638162.54641.322128.0289
139.53924.08476.54254.07426.04328.8965-0.48491.0612-0.9539-0.49790.7246-0.268-0.84330.6901-0.10410.436-0.04760.16140.5741-0.02620.426756.2536-0.069718.4409
142.83553.74470.37925.48230.72827.37270.03040.9115-0.4081-0.8708-0.8170.0468-0.1108-0.58530.40940.29040.0612-0.09910.8009-0.24260.707444.5073-8.304710.4597
156.06696.98930.23078.0630.43352.38250.46990.3983-0.24631.7148-0.25010.0344-0.1993-1.2977-0.04090.60380.1507-0.17080.9139-0.32370.78837.445-7.79116.917
163.18630.1361-0.80456.81082.0887.1345-0.021-0.09050.89290.2318-1.0484-0.8138-0.63320.42750.65421.0278-0.1281-0.00710.71160.21060.487662.4155-10.785344.811
172.1908-1.8893-1.41586.7331.56814.1974-0.3381-0.36950.12141.2988-0.09431.1896-0.46960.43260.3961.01370.04640.15670.49720.12650.672251.5459-13.631651.0625
181.413-0.7374-3.00880.66052.42069.1093-1.9803-0.17390.5337-0.3599-0.21622.15720.7111-1.80722.18760.8132-0.03060.05171.21620.12583.068930.4602-11.096448.4962
192.19750.46151.18568.35436.81525.8595-1.9514-0.2612-0.29930.7554-0.93560.57771.2048-0.99922.03041.17570.10640.40471.12050.37231.993836.275-15.819856.8332
202.62261.50420.47756.83294.42437.7144-1.35970.4372-0.1943-0.2412-0.8271.8521.663-1.19291.9880.9636-0.26540.46380.99350.03752.246940.2183-21.53248.2283
214.42210.8303-1.99860.7953-0.933.9756-1.9406-1.1362-3.76540.60310.0515-1.52841.68562.20292.05711.555-0.40430.48591.68590.15251.56627.8885-15.554743.3683
223.6057-5.25912.11779.835-1.61182.2505-0.09250.3746-0.0371-0.6005-0.0281-0.2480.61840.35740.28910.6712-0.0453-0.05120.54240.24130.537262.822-19.75639.2158
233.3923-2.58993.20832.9393-0.10078.3856-0.0732-0.30060.1706-0.06510.29010.30570.1685-0.556-0.46730.422-0.15240.01820.33390.16140.455363.1054-29.625152.119
243.96293.04694.61582.0317-0.71177.74510.607-0.16390.2761-0.8059-0.28220.42560.3448-0.9261-0.3130.73990.08680.01850.55210.12230.369159.3556-38.124658.9886
258.00360.49080.94234.4222-0.30324.94360.2101-0.73771.2536-0.02290.1605-0.4031-0.8116-0.2084-0.12340.70670.01240.07710.2832-0.03750.570966.6722-34.317267.1586
263.5559-2.18991.16366.4951-0.75325.3378-0.4874-0.55640.146-0.44640.30470.01340.3673-0.31280.22960.80680.3471-0.18850.8264-0.79971.210134.8389-27.1175-8.9752
274.9196-2.3260.54797.1608-2.30693.3884-1.161-1.16571.7905-0.05120.5869-1.0128-0.605-1.3065-0.58030.69080.5036-0.2511.4143-1.0126-0.30125.0714-31.1745-3.0716
283.07970.4735-0.56272.06661.97083.6260.1112-0.2576-0.10060.8779-0.1167-0.2120.2869-0.73760.00891.04060.14930.03041.55220.06690.453215.4781-41.89014.5029
29-0.001-0.0026-0.00140.00250.00520.00960.392-0.97840.36120.5293-0.07010.0561-0.2462-0.2538-0.15910.96390.1657-0.01691.7196-0.28110.696939.2798-17.3134-14.2891
309.8543-0.59971.36264.2977-0.10838.8105-0.2241-1.3971-0.62690.3604-0.3346-0.478-0.22090.64630.58780.51050.3562-0.30091.3705-0.4121.154540.3287-27.83313.6086
313.1305-0.3002-2.33293.9990.55413.37550.5293-0.3317-0.14620.1054-0.2169-0.186-0.29130.9799-0.42160.42290.5767-0.27851.2023-0.81251.061531.8061-18.1565.8849
326.4047-3.203-5.94391.653.04225.61140.7753-0.47271.95840.35420.6404-1.2908-1.56921.2141-1.29380.6178-0.08770.08581.2134-0.48221.324529.4333.41499.1664
331.48420.3768-0.70397.3641-0.00671.7121-0.1372-0.55490.66090.8920.2646-0.3028-0.03650.2466-0.01430.68760.4706-0.14630.4952-0.91261.325624.1175-5.761513.7706
343.7462-2.2271-0.25521.90681.15414.6443-0.15991.46950.57480.75620.833-0.72030.0268-0.1943-0.31810.54040.20350.03730.7592-0.30861.341322.8571-4.39993.4213
350.0041-0.00970.00430.1418-0.08860.06130.5547-0.334-0.11081.2387-0.05240.3247-0.40690.4245-0.08340.8347-0.2414-0.02150.9030.35630.753261.36481.968241.2837
363.9077-0.0629-5.2635.05820.54487.0506-1.2016-0.4177-1.35921.3250.05430.85040.54560.18041.19921.36760.03480.31080.53210.04320.654644.622-1.172553.5704
376.6183-0.3571-1.79913.70322.21924.7299-0.1158-2.5882-0.85581.59310.1187-0.79480.86941.7090.49352.08410.17640.26161.02730.54120.765754.64651.670561.6171
384.53980.13082.38190.78511.66844.5145-0.523-2.2412-0.16560.4560.55660.1062-0.12671.37910.19041.64210.5188-0.21274.19631.20271.392273.3025-6.614468.1545
394.41431.7058-3.00033.56973.51159.5358-0.1141-2.4313-1.59730.74121.2371-0.6539-0.60572.3868-0.91492.14880.163-0.39073.83540.3181.127466.14050.240374.0815
400.4483-0.0356-0.10910.0360.03760.0729-1.1779-2.0650.3039-0.33241.3146-0.0087-0.14771.6492-0.13082.409-0.6057-0.1722.1534-0.30861.138970.51995.783465.5055
412.2507-0.29942.33732.48852.33515.35260.20630.25950.1846-0.02960.01160.5155-0.6090.0906-0.02551.02650.08040.08730.45520.04270.535650.99267.858746.1568
423.0773-3.1707-0.71115.56551.9550.9449-0.22390.35-0.06230.47590.2629-0.2453-0.0074-0.3004-0.01521.5104-0.12170.11380.55880.15540.420348.150315.950255.0877
432.3535-1.0637-0.22357.46280.58848.12150.3435-0.64560.0391-0.099-0.404-0.18111.518-1.1420.09850.9454-0.12210.13570.6907-0.0370.38140.214925.423964.2871
443.6312-0.5688-0.33982.6021-0.27144.2639-0.08930.0211.0898-0.06430.5798-0.2289-0.65810.5956-0.53011.1031-0.285-0.07890.71830.00121.187319.9338-22.508855.034
453.5451-1.932-1.30733.311-2.93296.55270.8631-1.05030.3295-0.3954-0.8159-0.76240.30320.7594-0.06071.0291-0.1498-0.0381.14180.12811.726138.8848-36.274853.4749
469.48244.13072.53812.4560.07472.25590.6260.18730.53140.4473-0.110.4001-0.029-0.2767-0.66060.6738-0.03150.14910.7952-0.17480.91911.3693-27.130462.2962
479.66388.34046.74487.71015.6319.4573-0.06570.1447-0.61740.44720.5597-0.64070.03030.4847-0.55350.8247-0.01710.21230.5083-0.16470.669.6688-34.548751.7214
482.0163-3.48556.10677.6717-0.05644.32990.5725-0.9487-0.1775-1.39330.0893-0.55090.8182-0.0134-0.66550.9287-0.03940.26290.7806-0.10650.45274.7222-43.35640.1343
492.0063.25954.40236.40431.24886.57770.2817-0.66711.4271-0.3495-0.47180.0304-0.82360.72190.03231.0969-0.04090.3140.6066-0.16470.4330.6093-36.730734.5951
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 4 through 48 )
2X-RAY DIFFRACTION2chain 'A' and (resid 49 through 53 )
3X-RAY DIFFRACTION3chain 'A' and (resid 54 through 70 )
4X-RAY DIFFRACTION4chain 'A' and (resid 71 through 87 )
5X-RAY DIFFRACTION5chain 'B' and (resid 2 through 6 )
6X-RAY DIFFRACTION6chain 'B' and (resid 7 through 25 )
7X-RAY DIFFRACTION7chain 'B' and (resid 26 through 53 )
8X-RAY DIFFRACTION8chain 'B' and (resid 54 through 70 )
9X-RAY DIFFRACTION9chain 'B' and (resid 71 through 86 )
10X-RAY DIFFRACTION10chain 'C' and (resid 4 through 48 )
11X-RAY DIFFRACTION11chain 'C' and (resid 49 through 87 )
12X-RAY DIFFRACTION12chain 'D' and (resid 2 through 25 )
13X-RAY DIFFRACTION13chain 'D' and (resid 26 through 46 )
14X-RAY DIFFRACTION14chain 'D' and (resid 47 through 70 )
15X-RAY DIFFRACTION15chain 'D' and (resid 71 through 87 )
16X-RAY DIFFRACTION16chain 'E' and (resid 2 through 25 )
17X-RAY DIFFRACTION17chain 'E' and (resid 26 through 48 )
18X-RAY DIFFRACTION18chain 'E' and (resid 49 through 53 )
19X-RAY DIFFRACTION19chain 'E' and (resid 54 through 66 )
20X-RAY DIFFRACTION20chain 'E' and (resid 67 through 81 )
21X-RAY DIFFRACTION21chain 'E' and (resid 82 through 86 )
22X-RAY DIFFRACTION22chain 'F' and (resid 4 through 25 )
23X-RAY DIFFRACTION23chain 'F' and (resid 26 through 53 )
24X-RAY DIFFRACTION24chain 'F' and (resid 54 through 70 )
25X-RAY DIFFRACTION25chain 'F' and (resid 71 through 87 )
26X-RAY DIFFRACTION26chain 'G' and (resid 4 through 25 )
27X-RAY DIFFRACTION27chain 'G' and (resid 26 through 47 )
28X-RAY DIFFRACTION28chain 'G' and (resid 48 through 82 )
29X-RAY DIFFRACTION29chain 'H' and (resid 2 through 6 )
30X-RAY DIFFRACTION30chain 'H' and (resid 7 through 25 )
31X-RAY DIFFRACTION31chain 'H' and (resid 26 through 48 )
32X-RAY DIFFRACTION32chain 'H' and (resid 49 through 53 )
33X-RAY DIFFRACTION33chain 'H' and (resid 54 through 66 )
34X-RAY DIFFRACTION34chain 'H' and (resid 67 through 85 )
35X-RAY DIFFRACTION35chain 'I' and (resid 2 through 6 )
36X-RAY DIFFRACTION36chain 'I' and (resid 7 through 25 )
37X-RAY DIFFRACTION37chain 'I' and (resid 26 through 47 )
38X-RAY DIFFRACTION38chain 'I' and (resid 48 through 53 )
39X-RAY DIFFRACTION39chain 'I' and (resid 54 through 66 )
40X-RAY DIFFRACTION40chain 'I' and (resid 67 through 83 )
41X-RAY DIFFRACTION41chain 'J' and (resid 3 through 25 )
42X-RAY DIFFRACTION42chain 'J' and (resid 26 through 48 )
43X-RAY DIFFRACTION43chain 'J' and (resid 49 through 85 )
44X-RAY DIFFRACTION44chain 'K' and (resid 2 through 46 )
45X-RAY DIFFRACTION45chain 'K' and (resid 47 through 82 )
46X-RAY DIFFRACTION46chain 'L' and (resid 4 through 25 )
47X-RAY DIFFRACTION47chain 'L' and (resid 26 through 48 )
48X-RAY DIFFRACTION48chain 'L' and (resid 49 through 70 )
49X-RAY DIFFRACTION49chain 'L' and (resid 71 through 86 )

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