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- PDB-4ydj: Crystal structure of broadly and potently neutralizing antibody 4... -

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Basic information

Entry
Database: PDB / ID: 4ydj
TitleCrystal structure of broadly and potently neutralizing antibody 44-VRC13.01 in complex with HIV-1 clade AE strain 93TH057 gp120
Components
  • Envelope glycoprotein gp160,Envelope glycoprotein gp160
  • HEAVY CHAIN OF ANTIBODY 44-VRC13.01
  • LIGHT CHAIN OF ANTIBODY 44-VRC13.01
KeywordsIMMUNE SYSTEM / Antibody / HIV-1
Function / homology
Function and homology information


symbiont-mediated perturbation of host defense response / positive regulation of plasma membrane raft polarization / positive regulation of receptor clustering / host cell endosome membrane / clathrin-dependent endocytosis of virus by host cell / viral protein processing / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell ...symbiont-mediated perturbation of host defense response / positive regulation of plasma membrane raft polarization / positive regulation of receptor clustering / host cell endosome membrane / clathrin-dependent endocytosis of virus by host cell / viral protein processing / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane / structural molecule activity / membrane
Similarity search - Function
HIV Envelope Protein Gp120; Chain G / Human immunodeficiency virus 1, Gp160, envelope glycoprotein / Envelope glycoprotein Gp160 / Retroviral envelope protein / Retroviral envelope protein GP41-like / Gp120 core superfamily / Envelope glycoprotein GP120 / Human immunodeficiency virus 1, envelope glycoprotein Gp120 / Beta Complex / Immunoglobulins ...HIV Envelope Protein Gp120; Chain G / Human immunodeficiency virus 1, Gp160, envelope glycoprotein / Envelope glycoprotein Gp160 / Retroviral envelope protein / Retroviral envelope protein GP41-like / Gp120 core superfamily / Envelope glycoprotein GP120 / Human immunodeficiency virus 1, envelope glycoprotein Gp120 / Beta Complex / Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
(R,R)-2,3-BUTANEDIOL / ISOPROPYL ALCOHOL / DI(HYDROXYETHYL)ETHER / Envelope glycoprotein gp160
Similarity search - Component
Biological speciesHuman immunodeficiency virus 1
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.308 Å
AuthorsZhou, T. / Moquin, S. / Kwong, P.D.
CitationJournal: Cell / Year: 2015
Title: Structural Repertoire of HIV-1-Neutralizing Antibodies Targeting the CD4 Supersite in 14 Donors.
Authors: Zhou, T. / Lynch, R.M. / Chen, L. / Acharya, P. / Wu, X. / Doria-Rose, N.A. / Joyce, M.G. / Lingwood, D. / Soto, C. / Bailer, R.T. / Ernandes, M.J. / Kong, R. / Longo, N.S. / Louder, M.K. / ...Authors: Zhou, T. / Lynch, R.M. / Chen, L. / Acharya, P. / Wu, X. / Doria-Rose, N.A. / Joyce, M.G. / Lingwood, D. / Soto, C. / Bailer, R.T. / Ernandes, M.J. / Kong, R. / Longo, N.S. / Louder, M.K. / McKee, K. / O'Dell, S. / Schmidt, S.D. / Tran, L. / Yang, Z. / Druz, A. / Luongo, T.S. / Moquin, S. / Srivatsan, S. / Yang, Y. / Zhang, B. / Zheng, A. / Pancera, M. / Kirys, T. / Georgiev, I.S. / Gindin, T. / Peng, H.P. / Yang, A.S. / Mullikin, J.C. / Gray, M.D. / Stamatatos, L. / Burton, D.R. / Koff, W.C. / Cohen, M.S. / Haynes, B.F. / Casazza, J.P. / Connors, M. / Corti, D. / Lanzavecchia, A. / Sattentau, Q.J. / Weiss, R.A. / West, A.P. / Bjorkman, P.J. / Scheid, J.F. / Nussenzweig, M.C. / Shapiro, L. / Mascola, J.R. / Kwong, P.D.
History
DepositionFeb 22, 2015Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 3, 2015Provider: repository / Type: Initial release
Revision 1.1Jun 10, 2015Group: Database references
Revision 1.2Jun 17, 2015Group: Database references
Revision 1.3Jul 29, 2020Group: Data collection / Derived calculations ...Data collection / Derived calculations / Source and taxonomy / Structure summary
Category: chem_comp / entity ...chem_comp / entity / entity_src_gen / pdbx_chem_comp_identifier / pdbx_entity_nonpoly / pdbx_struct_assembly / pdbx_struct_assembly_gen / pdbx_struct_oper_list / struct_conn / struct_site / struct_site_gen
Item: _chem_comp.name / _chem_comp.type ..._chem_comp.name / _chem_comp.type / _entity.pdbx_description / _entity_src_gen.pdbx_alt_source_flag / _pdbx_entity_nonpoly.name / _pdbx_struct_assembly.oligomeric_details / _pdbx_struct_assembly_gen.asym_id_list / _pdbx_struct_oper_list.symmetry_operation / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.pdbx_ptnr1_PDB_ins_code / _struct_conn.pdbx_role / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 1.4Sep 27, 2023Group: Data collection / Database references ...Data collection / Database references / Refinement description / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.5Nov 13, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
G: Envelope glycoprotein gp160,Envelope glycoprotein gp160
H: HEAVY CHAIN OF ANTIBODY 44-VRC13.01
L: LIGHT CHAIN OF ANTIBODY 44-VRC13.01
I: Envelope glycoprotein gp160,Envelope glycoprotein gp160
A: HEAVY CHAIN OF ANTIBODY 44-VRC13.01
B: LIGHT CHAIN OF ANTIBODY 44-VRC13.01
hetero molecules


Theoretical massNumber of molelcules
Total (without water)179,62840
Polymers173,8446
Non-polymers5,78434
Water6,756375
1
G: Envelope glycoprotein gp160,Envelope glycoprotein gp160
H: HEAVY CHAIN OF ANTIBODY 44-VRC13.01
L: LIGHT CHAIN OF ANTIBODY 44-VRC13.01
hetero molecules


Theoretical massNumber of molelcules
Total (without water)89,58918
Polymers86,9223
Non-polymers2,66715
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
I: Envelope glycoprotein gp160,Envelope glycoprotein gp160
A: HEAVY CHAIN OF ANTIBODY 44-VRC13.01
B: LIGHT CHAIN OF ANTIBODY 44-VRC13.01
hetero molecules


Theoretical massNumber of molelcules
Total (without water)90,03922
Polymers86,9223
Non-polymers3,11819
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)293.796, 67.053, 93.638
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number18
Space group name H-MP21212

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Components

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Antibody , 2 types, 4 molecules HALB

#2: Antibody HEAVY CHAIN OF ANTIBODY 44-VRC13.01


Mass: 25534.984 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Plasmid: pVRC8400 / Cell line (production host): HEK293F / Production host: Homo sapiens (human)
#3: Antibody LIGHT CHAIN OF ANTIBODY 44-VRC13.01


Mass: 22175.512 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Plasmid: pVRC8400 / Cell line (production host): HEK293F / Production host: Homo sapiens (human)

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Protein / Sugars , 2 types, 24 molecules GI

#1: Protein Envelope glycoprotein gp160,Envelope glycoprotein gp160


Mass: 39211.434 Da / Num. of mol.: 2 / Fragment: UNP residues 43-122, 201-303, 325-486
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human immunodeficiency virus 1 / Strain: 93TH057 / Gene: env / Plasmid: pVRC8400 / Cell line (production host): HEK 293 GNTI- / Production host: Homo sapiens (human) / References: UniProt: Q0ED31
#4: Sugar...
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 22 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 6 types, 387 molecules

#5: Chemical ChemComp-IPA / ISOPROPYL ALCOHOL / 2-PROPANOL


Mass: 60.095 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C3H8O
#6: Chemical
ChemComp-BU3 / (R,R)-2,3-BUTANEDIOL


Mass: 90.121 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C4H10O2
#7: Chemical ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Na
#8: Chemical ChemComp-PEG / DI(HYDROXYETHYL)ETHER


Mass: 106.120 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C4H10O3
#9: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Cl
#10: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 375 / Source method: isolated from a natural source / Formula: H2O

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Details

Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.65 Å3/Da / Density % sol: 53.62 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.5
Details: 9.5% PEG 8000, 15% isopropanol, 0.1M imidazole, pH 6.5
PH range: 6.5

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-BM / Wavelength: 1 Å
DetectorType: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Mar 13, 2012
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.3→50 Å / Num. obs: 81592 / % possible obs: 99.3 % / Redundancy: 5 % / Biso Wilson estimate: 52.87 Å2 / Rmerge(I) obs: 0.141 / Χ2: 1.587 / Net I/av σ(I): 13.386 / Net I/σ(I): 7.3 / Num. measured all: 410786
Reflection shell

Diffraction-ID: 1 / Rejects: _

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique allΧ2% possible all
2.3-2.343.20.83338380.88695.2
2.34-2.383.70.84939940.86297.6
2.38-2.434.20.86239650.91599.1
2.43-2.484.60.79140410.91799.7
2.48-2.5350.76740400.94399.9
2.53-2.595.20.69640581.00899.8
2.59-2.665.30.5740751.059100
2.66-2.735.30.49340261.15199.9
2.73-2.815.30.4240691.27599.8
2.81-2.95.30.33540891.42499.8
2.9-35.30.27640751.598100
3-3.125.40.24340501.74599.9
3.12-3.265.30.20540862.06199.9
3.26-3.445.30.18340982.29299.8
3.44-3.655.30.1641132.412100
3.65-3.935.30.14741462.31499.9
3.93-4.335.40.13241092.21599.8
4.33-4.955.40.12141852.11799.6
4.95-6.245.50.1142031.76199.5
6.24-505.20.09243321.64997.3

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassification
PHENIXdev_1702refinement
HKL-2000data reduction
SCALEPACKdata scaling
PDB_EXTRACT3.15data extraction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3SE9

3se9


Resolution: 2.308→38.3 Å / SU ML: 0.3 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 27.09 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.2495 4075 5 %Random selection
Rwork0.2233 77390 --
obs0.2246 81465 98.89 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 334.7 Å2 / Biso mean: 98.2266 Å2 / Biso min: 30.82 Å2
Refinement stepCycle: final / Resolution: 2.308→38.3 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11354 0 747 375 12476
Biso mean--97.94 63.66 -
Num. residues----1476
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00312020
X-RAY DIFFRACTIONf_angle_d0.73116328
X-RAY DIFFRACTIONf_chiral_restr0.0351897
X-RAY DIFFRACTIONf_plane_restr0.0042081
X-RAY DIFFRACTIONf_dihedral_angle_d11.9094307
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 29

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.3084-2.33550.36431210.32412226234784
2.3355-2.3640.31071150.31292548266396
2.364-2.39390.31411340.2932650278498
2.3939-2.42540.32491490.28892579272899
2.4254-2.45860.28841440.27732637278199
2.4586-2.49370.26461220.265526882810100
2.4937-2.5310.30471370.279126532790100
2.531-2.57050.28451560.274326722828100
2.5705-2.61260.27791530.28826282781100
2.6126-2.65770.31331440.271126572801100
2.6577-2.7060.31111450.267526702815100
2.706-2.7580.31751470.278526732820100
2.758-2.81430.30651670.263226482815100
2.8143-2.87550.29031610.268226422803100
2.8755-2.94230.32161270.253326772804100
2.9423-3.01590.28531330.251527002833100
3.0159-3.09740.24781470.247326482795100
3.0974-3.18850.311310.249127192850100
3.1885-3.29140.3031280.248126872815100
3.2914-3.40890.26311480.244126772825100
3.4089-3.54530.25121560.225126942850100
3.5453-3.70660.23441360.21427092845100
3.7066-3.90180.24191300.218727232853100
3.9018-4.1460.22561530.207726912844100
4.146-4.46560.2291340.1827492883100
4.4656-4.91420.19131280.171227272855100
4.9142-5.62340.19171490.180927552904100
5.6234-7.07760.24271330.22652792292599
7.0776-38.30480.23021470.20892871301897
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.72770.59560.75878.47942.87922.9518-0.1219-1.39460.01740.4752-0.0733-0.051-0.5273-0.15210.15411.3449-0.1844-0.12751.68030.34880.8609-22.591228.3104-24.1784
25.37771.0775-1.2174.1737-0.07685.825-0.1096-1.3819-1.23630.63340.1121-0.18660.51090.17160.06590.8373-0.0178-0.0151.33340.48960.9698-19.123621.4947-35.7415
34.98361.50780.49533.23521.21224.2962-0.159-0.63420.15910.276-0.0061-0.34970.36180.73810.16890.37440.0803-0.09231.0920.18810.67-16.270333.388-45.0315
40.3233-1.16520.36159.35922.95488.8752-0.311-0.26090.6688-0.20370.504-1.8741-0.79221.3311-0.00050.4257-0.0799-0.09491.03180.08281.1702-11.367843.8395-52.0326
52.0015.23632.3348.1905-2.52933.0497-0.20270.8677-2.0586-0.67521.5635-1.4667-0.10790.9837-1.21630.99210.2110.28281.4406-0.03481.3-10.930327.7802-63.9656
64.7261-0.3578-1.67684.780.24143.8337-0.1821-0.12910.65710.1195-0.2874-0.0109-0.31640.20490.44290.49230.0262-0.13781.07330.1420.8819-24.263345.0362-49.622
76.1111-2.1986-6.42810.80292.31456.7614-0.01732.57340.6093-0.55790.2108-0.5669-1.3977-2.0231-0.09721.774-0.14240.14741.46480.21931.1036-10.633744.4442-62.5885
85.7981-4.8875-2.21674.17281.88240.8752-0.88410.89480.09441.0517-0.0561-0.06790.0110.29290.82551.4451-0.3185-0.14811.67660.27211.3442-4.828150.8431-61.0336
94.14030.6686-4.23876.3936-1.15184.2362-0.05091.06340.5557-0.52780.0265-0.67-0.2461-0.1380.04930.69150.0572-0.28591.02680.17341.1702-23.451252.1849-47.254
106.4863-1.9155-0.41588.68284.5976.52660.099-1.83891.14320.56870.5416-1.063-0.1680.9894-0.46520.5847-0.0457-0.0281.312-0.10530.9187-16.44446.1117-41.4197
119.52480.23432.59982.39160.98142.7291-0.12681.17550.2833-1.812-0.04020.98770.2493-0.6530.16530.9926-0.0166-0.1121.19430.16690.8684-20.055230.1006-66.3311
124.52961.1725-1.55492.7916-1.24463.3904-0.2036-1.1428-0.2954-0.196-0.1-0.17441.1374-0.22810.37330.6901-0.0152-0.01651.11590.20920.8981-21.905926.2021-42.4188
134.6649-1.92622.45735.7916-1.81714.2487-0.1375-0.37740.2018-0.10020.1568-0.1034-0.13460.1877-0.01380.3368-0.0834-0.02740.64490.08130.5367-43.726547.5922-65.5278
144.77541.01551.37027.65880.81092.32830.08630.0673-0.10680.3953-0.16720.17870.0022-0.06090.08720.57-0.00520.04860.29360.02430.2738-77.959847.1104-90.2877
154.23664.43785.02994.53465.21285.9802-0.78792.1699-0.8975-1.74080.6807-2.2322-1.06981.95440.09250.935-0.23520.221.20780.20191.0723-48.023629.243-79.4672
163.3172-0.51491.84390.7317-1.03113.87960.2739-0.1722-0.4159-0.0948-0.1587-0.16850.26170.3848-0.12640.57610.02310.00970.38810.0350.4945-53.10830.4465-76.0181
172.51470.4868-0.08049.0323-3.57193.9140.1650.1020.1820.0682-0.4255-0.4041-0.39230.1860.22730.45790.04180.06510.29120.01360.4016-63.874936.0564-94.0102
187.96533.76631.91845.69130.78964.12410.4549-1.5634-0.39091.5708-0.4935-0.3248-0.2944-1.3836-0.00471.31230.07580.04611.85930.31040.9061-21.6545-7.507220.8946
195.1625-0.5691.10953.9685-0.77346.2691-0.4769-1.7612-1.04630.70720.1149-0.30280.4909-0.06330.32570.80380.1202-0.10071.65740.58531.2113-20.0615-10.269910.7514
204.98640.46971.51174.5-0.45514.8818-0.2682-0.86130.2870.2129-0.0622-1.1917-0.23480.40930.370.47080.0937-0.14091.03590.1290.8925-16.36663.5254-2.4811
213.28010.97051.78754.6855-0.81547.4427-0.81580.6441.9959-0.41790.0553-0.5637-0.5833-0.24710.69040.85310.0117-0.36290.9230.09021.0707-16.470316.4884-8.0385
220.1991.120.4449.10555.91365.2364-0.0787-1.61170.40081.17780.25-1.4955-0.02620.9356-0.21840.71320.0236-0.30251.4023-0.25341.0673-15.506812.88074.7263
236.48915.2831-4.36599.0797-6.99125.5906-0.4062-0.22890.1514-1.4927-0.1272-0.77671.03090.49890.62510.68860.1278-0.07290.80020.10750.8393-22.0609-0.8032-16.5335
245.5501-2.4644-3.85843.87781.30212.75961.37030.1474-0.3055-0.0097-1.0597-0.3140.49031.1634-0.24370.68010.1685-0.1271.54440.26720.9362-23.1458-3.2174.7066
250.5856-0.54040.70063.08113.62228.0366-0.1149-1.1494-0.22031.7180.32560.05361.2178-1.2581-0.25481.24170.5050.09932.14320.81091.3114-19.6116-17.759911.7738
264.8356-2.11052.73185.3005-2.01314.0026-0.3871-0.33930.43130.51670.1699-0.36-0.44060.21530.19060.4385-0.0467-0.10220.5669-0.00920.4771-43.694917.1543-18.381
273.95942.00621.44847.35871.76313.5364-0.0720.1396-0.0890.112-0.0994-0.04890.0286-0.04290.17180.27960.00780.01530.20680.03580.2434-78.172218.2029-43.7473
282.5157-3.08331.17824.6169-1.28978.16960.05761.06780.657-0.2406-0.0745-0.1948-0.01220.79790.04640.59320.07980.05040.480.11920.498-50.3164-1.2027-31.2792
294.6462.72750.65976.73551.10843.74540.0932-0.3803-0.57120.8195-0.1013-0.51110.59510.4467-0.01380.73460.1051-0.05060.48890.11970.4527-47.8258-3.7546-20.6742
302.4599-1.14752.08422.5978-1.52064.38410.0818-0.0731-0.09360.1825-0.04820.00130.25390.1598-0.04040.4239-0.02210.05190.2402-0.05450.3406-60.79284.3797-33.9367
311.85481.7514-2.25288.4358-5.8369.55260.20.00010.05550.341-0.2832-0.2074-0.37480.21950.10040.326-0.0253-0.10380.31750.00620.3715-64.13748.2602-44.4829
326.29171.0822-3.01172.66461.26832.75450.41640.6492-0.1246-0.9459-0.21720.10580.70950.2396-0.58940.569-0.0473-0.01960.3248-0.07460.3319-66.20462.7354-52.212
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'G' and (resid 44 through 82 )G0
2X-RAY DIFFRACTION2chain 'G' and (resid 83 through 245 )G0
3X-RAY DIFFRACTION3chain 'G' and (resid 246 through 299 )G0
4X-RAY DIFFRACTION4chain 'G' and (resid 327 through 349 )G0
5X-RAY DIFFRACTION5chain 'G' and (resid 350 through 360 )G0
6X-RAY DIFFRACTION6chain 'G' and (resid 361 through 391 )G0
7X-RAY DIFFRACTION7chain 'G' and (resid 392 through 397 )G0
8X-RAY DIFFRACTION8chain 'G' and (resid 407 through 411 )G0
9X-RAY DIFFRACTION9chain 'G' and (resid 412 through 423 )G0
10X-RAY DIFFRACTION10chain 'G' and (resid 441 through 453 )G0
11X-RAY DIFFRACTION11chain 'G' and (resid 454 through 467 )G0
12X-RAY DIFFRACTION12chain 'G' and (resid 468 through 491 )G0
13X-RAY DIFFRACTION13chain 'H' and (resid 1 through 112 )H0
14X-RAY DIFFRACTION14chain 'H' and (resid 113 through 216 )H0
15X-RAY DIFFRACTION15chain 'L' and (resid 1 through 14 )L0
16X-RAY DIFFRACTION16chain 'L' and (resid 15 through 137 )L0
17X-RAY DIFFRACTION17chain 'L' and (resid 138 through 211 )L0
18X-RAY DIFFRACTION18chain 'I' and (resid 44 through 82 )I0
19X-RAY DIFFRACTION19chain 'I' and (resid 83 through 235 )I0
20X-RAY DIFFRACTION20chain 'I' and (resid 236 through 385 )I0
21X-RAY DIFFRACTION21chain 'I' and (resid 386 through 423 )I0
22X-RAY DIFFRACTION22chain 'I' and (resid 441 through 453 )I0
23X-RAY DIFFRACTION23chain 'I' and (resid 454 through 474 )I0
24X-RAY DIFFRACTION24chain 'I' and (resid 475 through 483 )I0
25X-RAY DIFFRACTION25chain 'I' and (resid 484 through 492 )I0
26X-RAY DIFFRACTION26chain 'A' and (resid 1 through 111 )A0
27X-RAY DIFFRACTION27chain 'A' and (resid 112 through 216 )A0
28X-RAY DIFFRACTION28chain 'B' and (resid 1 through 14 )B0
29X-RAY DIFFRACTION29chain 'B' and (resid 15 through 75 )B0
30X-RAY DIFFRACTION30chain 'B' and (resid 76 through 129 )B0
31X-RAY DIFFRACTION31chain 'B' and (resid 130 through 197 )B0
32X-RAY DIFFRACTION32chain 'B' and (resid 198 through 211 )B0

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