Mass: 26000.039 Da / Num. of mol.: 1 / Fragment: HEAVY CHAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Cell line (production host): HEK293 EBNA / Production host: HOMO SAPIENS (human)
#2: Antibody
FPRO0165FAB
Mass: 23953.803 Da / Num. of mol.: 1 / Fragment: LIGHT CHAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Cell line (production host): HEK293 EBNA / Production host: HOMO SAPIENS (human)
-
Experimental details
-
Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
-
Sample preparation
Crystal
Density Matthews: 4.3 Å3/Da / Density % sol: 71 % / Description: NONE
Crystal grow
Details: 0.1 M TRIS-HCL PH 7.5-8.0, 18% PEG8000, 0.18-0.2 M MGCL2
Resolution: 3.08→39.2 Å / Cor.coef. Fo:Fc: 0.8321 / Cor.coef. Fo:Fc free: 0.7915 / SU R Cruickshank DPI: 0.856 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.796 / SU Rfree Blow DPI: 0.385 / SU Rfree Cruickshank DPI: 0.396 Details: REFINEMENT WAS PERFORMED USIN RESOLUTION FAB FRAGMENT STRUCTURES WITH HIGH HOMOLOGY TO FPR0165 FAB WERE USED TO BUILD A MODEL FOR TARGET RESTRAINTS, PDB CODE 3AAZ AND PDB CODE 3GHB FOR THE ...Details: REFINEMENT WAS PERFORMED USIN RESOLUTION FAB FRAGMENT STRUCTURES WITH HIGH HOMOLOGY TO FPR0165 FAB WERE USED TO BUILD A MODEL FOR TARGET RESTRAINTS, PDB CODE 3AAZ AND PDB CODE 3GHB FOR THE LIGHT AND HEAVY CHAINS RESPECTIVELY.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.2804
812
5.02 %
RANDOM
Rwork
0.2427
-
-
-
obs
0.2446
16180
99.58 %
-
Displacement parameters
Biso mean: 111.81 Å2
Baniso -1
Baniso -2
Baniso -3
1-
-29.3105 Å2
0 Å2
0 Å2
2-
-
-29.3105 Å2
0 Å2
3-
-
-
58.621 Å2
Refine analyze
Luzzati coordinate error obs: 0.826 Å
Refinement step
Cycle: LAST / Resolution: 3.08→39.2 Å
Protein
Nucleic acid
Ligand
Solvent
Total
Num. atoms
3398
0
0
0
3398
Refine LS restraints
Refine-ID
Type
Dev ideal
Number
Restraint function
Weight
X-RAY DIFFRACTION
t_bond_d
0.01
3485
HARMONIC
2
X-RAY DIFFRACTION
t_angle_deg
1.27
4746
HARMONIC
2
X-RAY DIFFRACTION
t_dihedral_angle_d
1140
SINUSOIDAL
2
X-RAY DIFFRACTION
t_incorr_chiral_ct
X-RAY DIFFRACTION
t_pseud_angle
X-RAY DIFFRACTION
t_trig_c_planes
73
HARMONIC
2
X-RAY DIFFRACTION
t_gen_planes
505
HARMONIC
5
X-RAY DIFFRACTION
t_it
3485
HARMONIC
20
X-RAY DIFFRACTION
t_nbd
X-RAY DIFFRACTION
t_omega_torsion
3.64
X-RAY DIFFRACTION
t_other_torsion
22.39
X-RAY DIFFRACTION
t_improper_torsion
X-RAY DIFFRACTION
t_chiral_improper_torsion
452
SEMIHARMONIC
5
X-RAY DIFFRACTION
t_sum_occupancies
X-RAY DIFFRACTION
t_utility_distance
X-RAY DIFFRACTION
t_utility_angle
X-RAY DIFFRACTION
t_utility_torsion
X-RAY DIFFRACTION
t_ideal_dist_contact
3938
SEMIHARMONIC
4
LS refinement shell
Resolution: 3.08→3.29 Å / Total num. of bins used: 8
Rfactor
Num. reflection
% reflection
Rfree
0.2765
149
5.26 %
Rwork
0.2878
2685
-
all
0.2872
2834
-
obs
-
-
99.58 %
+
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