Mass: 25965.783 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: IGHG1 / Cell line (production host): HEK293F / Production host: Homo sapiens (human) / References: UniProt: P01857
#2: Antibody
APE1551AbFablightchain
Mass: 24342.928 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK293F / Production host: Homo sapiens (human) / References: UniProt: Q8TCD0
Resolution: 1.6→1.63 Å / Redundancy: 5 % / Rmerge(I) obs: 0.623 / Mean I/σ(I) obs: 2.4 / Num. unique all: 3210 / % possible all: 96.3
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Processing
Software
Name
Version
Classification
Web-Ice
datacollection
PHASER
phasing
REFMAC
5.7.0029
refinement
HKL-2000
datareduction
HKL-2000
datascaling
Refinement
Method to determine structure: MOLECULAR REPLACEMENT Starting model: APE1531 Fab Resolution: 1.602→45.58 Å / Cor.coef. Fo:Fc: 0.973 / Cor.coef. Fo:Fc free: 0.962 / SU B: 3.701 / SU ML: 0.064 / Cross valid method: THROUGHOUT / ESU R: 0.083 / ESU R Free: 0.085 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.19767
3280
5.1 %
RANDOM
Rwork
0.16628
-
-
-
obs
0.16787
61414
97.83 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK