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Open data
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Basic information
Entry | Database: PDB / ID: 1sbs | ||||||
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Title | CRYSTAL STRUCTURE OF AN ANTI-HCG FAB | ||||||
![]() | (MONOCLONAL ANTIBODY 3A2) x 2 | ||||||
![]() | MONOCLONAL ANTIBODY / FAB-FRAGMENT / REPRODUCTION | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta / : / : ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fotinou, C. / Beauchamp, J. / Emsley, P. / Dehaan, A. / Schielen, W.J.G. / Bos, E. / Isaacs, N.W. | ||||||
![]() | ![]() Title: Structure of an Fab fragment against a C-terminal peptide of hCG at 2.0 A resolution. Authors: Fotinou, C. / Beauchamp, J. / Emsley, P. / deHaan, A. / Schielen, W.J. / Bos, E. / Isaacs, N.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 118.4 KB | Display | ![]() |
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PDB format | ![]() | 92.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 391.8 KB | Display | ![]() |
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Full document | ![]() | 403.4 KB | Display | |
Data in XML | ![]() | 12 KB | Display | |
Data in CIF | ![]() | 23.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1igfS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 |
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Unit cell |
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Components
#1: Antibody | Mass: 24131.965 Da / Num. of mol.: 1 / Fragment: FAB FRAGMENT / Source method: isolated from a natural source / Details: HYBRIDOMA CELL / Source: (natural) ![]() ![]() |
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#2: Antibody | Mass: 24426.975 Da / Num. of mol.: 1 / Fragment: FAB FRAGMENT / Source method: isolated from a natural source / Details: HYBRIDOMA CELL / Source: (natural) ![]() ![]() |
#3: Chemical | ChemComp-SO4 / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.7 Å3/Da / Density % sol: 66 % | ||||||||||||||||||||
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Crystal grow | pH: 7 / Details: pH 7.00 | ||||||||||||||||||||
Crystal grow | *PLUS Method: unknown | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Oct 1, 1994 / Details: MIRRORS |
Radiation | Monochromator: SI(111) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. obs: 43925 / % possible obs: 99 % / Redundancy: 4 % / Rmerge(I) obs: 0.091 / Net I/σ(I): 6.2 |
Reflection shell | Resolution: 2→2.05 Å / Redundancy: 2.8 % / Rmerge(I) obs: 0.365 / Mean I/σ(I) obs: 1.8 / % possible all: 93 |
Reflection | *PLUS Num. measured all: 174451 |
Reflection shell | *PLUS % possible obs: 93 % |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: 1IGF Resolution: 2→20 Å / Cross valid method: THROUGHOUT Details: POSITIONAL REFINEMENT WITH X-PLOR. R_CRYS=36%, R_FREE=41%. UNRESTRAINED B-FACTOR REFINEMENT USING SFALL. R_CRYS=23.8%, R_FREE=33.8%. THE REFINEMENT CONTINUED USING ONLY REFMAC. R_CRYS=18.2%, ...Details: POSITIONAL REFINEMENT WITH X-PLOR. R_CRYS=36%, R_FREE=41%. UNRESTRAINED B-FACTOR REFINEMENT USING SFALL. R_CRYS=23.8%, R_FREE=33.8%. THE REFINEMENT CONTINUED USING ONLY REFMAC. R_CRYS=18.2%, R_FREE=24.1% THE SEARCHING FOR WATER POSITIONS WAS PERFORMED USING ARP.
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Refinement step | Cycle: LAST / Resolution: 2→20 Å
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||
Displacement parameters | *PLUS |