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TitleNucleotide insertions and deletions complement point mutations to massively expand the diversity created by somatic hypermutation of antibodies.
Journal, issue, pagesJ. Biol. Chem., Vol. 289, Page 33557-33567, Year 2014
Publish dateDec 6, 2013 (structure data deposition date)
AuthorsBowers, P.M. / Verdino, P. / Wang, Z. / da Silva Correia, J. / Chhoa, M. / Macondray, G. / Do, M. / Neben, T.Y. / Horlick, R.A. / Stanfield, R.L. ...Bowers, P.M. / Verdino, P. / Wang, Z. / da Silva Correia, J. / Chhoa, M. / Macondray, G. / Do, M. / Neben, T.Y. / Horlick, R.A. / Stanfield, R.L. / Wilson, I.A. / King, D.J.
External linksJ. Biol. Chem. / PubMed:25320089
MethodsX-ray diffraction
Resolution1.602 - 1.75 Å
Structure data

PDB-4nwt:
Crystal structure of the anti-human NGF Fab APE1531
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-4nwu:
Crystal structure of APE1551, an anti-human NGF Fab with a nine amino acid insertion in CDR H1
Method: X-RAY DIFFRACTION / Resolution: 1.602 Å

Chemicals

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-ACT:
ACETATE ION

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsIMMUNE SYSTEM / beta sandwich / human beta nerve growth factor / beta-sandwich

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