[English] 日本語
![](img/lk-miru.gif)
- PDB-3ivq: Structures of SPOP-Substrate Complexes: Insights into Molecular A... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 3ivq | ||||||
---|---|---|---|---|---|---|---|
Title | Structures of SPOP-Substrate Complexes: Insights into Molecular Architectures of BTB-Cul3 Ubiquitin Ligases: SPOPMATH-CiSBC2 | ||||||
![]() |
| ||||||
![]() | LIGASE / Protein Binding / Nucleus / Ubl conjugation pathway | ||||||
Function / homology | ![]() regulation of proteolysis / Cul3-RING ubiquitin ligase complex / molecular function inhibitor activity / Hedgehog 'on' state / protein polyubiquitination / proteasome-mediated ubiquitin-dependent protein catabolic process / nuclear speck / ubiquitin protein ligase binding / nucleoplasm / identical protein binding ...regulation of proteolysis / Cul3-RING ubiquitin ligase complex / molecular function inhibitor activity / Hedgehog 'on' state / protein polyubiquitination / proteasome-mediated ubiquitin-dependent protein catabolic process / nuclear speck / ubiquitin protein ligase binding / nucleoplasm / identical protein binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Schulman, B.A. / Miller, D.J. / Calabrese, M.F. / Seyedin, S. | ||||||
![]() | ![]() Title: Structures of SPOP-Substrate Complexes: Insights into Molecular Architectures of BTB-Cul3 Ubiquitin Ligases. Authors: Zhuang, M. / Calabrese, M.F. / Liu, J. / Waddell, M.B. / Nourse, A. / Hammel, M. / Miller, D.J. / Walden, H. / Duda, D.M. / Seyedin, S.N. / Hoggard, T. / Harper, J.W. / White, K.P. / Schulman, B.A. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 73.1 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 54.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 3hqhC ![]() 3hqiC ![]() 3hqlC ![]() 3hqmC ![]() 3hsvC ![]() 3htmC ![]() 3hu6C ![]() 3hveC ![]() 3ivvC C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 16543.967 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein/peptide | Mass: 1305.371 Da / Num. of mol.: 2 / Source method: obtained synthetically #3: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 1.83 Å3/Da / Density % sol: 32.69 % |
---|---|
Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.6 Details: 28% PME 550, 0.1 M BTP, Cryoprotection: 35% PME 550, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→50 Å / Num. all: 15678 / Num. obs: 15674 / % possible obs: 100 % / Observed criterion σ(F): -3 / Observed criterion σ(I): 0 / Redundancy: 7.2 % / Rsym value: 0.077 / Net I/σ(I): 26 |
Reflection shell | Resolution: 2.1→2.18 Å / Redundancy: 7.3 % / Mean I/σ(I) obs: 4.4 / Num. unique all: 1558 / Rsym value: 0.365 / % possible all: 100 |
-
Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| ||||||||||||||||||||
Displacement parameters | Biso mean: 40.6 Å2
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→40 Å
| ||||||||||||||||||||
Refine LS restraints |
|