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- PDB-2lb3: Structure of the WW domain of PIN1 in complex with a human phosph... -
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Basic information
Entry | Database: PDB / ID: 2lb3 | ||||||
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Title | Structure of the WW domain of PIN1 in complex with a human phosphorylated Smad3 derived peptide | ||||||
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![]() | SIGNALING PROTEIN/TRANSCRIPTION / PIN1 / SMAD / CDK / signal transduction / SIGNALING PROTEIN-TRANSCRIPTION complex | ||||||
Function / homology | ![]() zygotic specification of dorsal/ventral axis / regulation of binding / homomeric SMAD protein complex / paraxial mesoderm morphogenesis / activin responsive factor complex / SMAD4 MH2 Domain Mutants in Cancer / SMAD2/3 MH2 Domain Mutants in Cancer / nodal signaling pathway / SMAD protein complex / endoderm formation ...zygotic specification of dorsal/ventral axis / regulation of binding / homomeric SMAD protein complex / paraxial mesoderm morphogenesis / activin responsive factor complex / SMAD4 MH2 Domain Mutants in Cancer / SMAD2/3 MH2 Domain Mutants in Cancer / nodal signaling pathway / SMAD protein complex / endoderm formation / co-SMAD binding / heteromeric SMAD protein complex / determination of left/right asymmetry in lateral mesoderm / odontoblast differentiation / secondary palate development / pericardium development / FOXO-mediated transcription of cell cycle genes / SMAD2/3 Phosphorylation Motif Mutants in Cancer / TGFBR1 KD Mutants in Cancer / cis-trans isomerase activity / Transcriptional regulation of pluripotent stem cells / regulation of transforming growth factor beta receptor signaling pathway / embryonic foregut morphogenesis / phosphothreonine residue binding / primary miRNA processing / transforming growth factor beta receptor binding / Germ layer formation at gastrulation / pulmonary valve morphogenesis / negative regulation of cell motility / type I transforming growth factor beta receptor binding / Formation of definitive endoderm / signal transduction involved in regulation of gene expression / activin receptor signaling pathway / ubiquitin ligase activator activity / Signaling by Activin / SMAD protein signal transduction / Formation of axial mesoderm / positive regulation of BMP signaling pathway / Signaling by NODAL / response to cholesterol / embryonic cranial skeleton morphogenesis / regulation of protein localization to nucleus / I-SMAD binding / aortic valve morphogenesis / pancreas development / GTPase activating protein binding / insulin secretion / postsynaptic cytosol / anterior/posterior pattern specification / mitogen-activated protein kinase kinase binding / ureteric bud development / endocardial cushion morphogenesis / regulation of mitotic nuclear division / organ growth / adrenal gland development / negative regulation of SMAD protein signal transduction / SMAD binding / PI5P Regulates TP53 Acetylation / TGF-beta receptor signaling activates SMADs / negative regulation of amyloid-beta formation / cytoskeletal motor activity / R-SMAD binding / mesoderm formation / phosphoserine residue binding / RHO GTPases Activate NADPH Oxidases / phosphatase binding / anatomical structure morphogenesis / cell fate commitment / cis-regulatory region sequence-specific DNA binding / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / protein peptidyl-prolyl isomerization / positive regulation of epithelial to mesenchymal transition / positive regulation of protein dephosphorylation / gastrulation / Downregulation of TGF-beta receptor signaling / transforming growth factor beta receptor signaling pathway / ciliary basal body / post-embryonic development / positive regulation of GTPase activity / regulation of cytokinesis / negative regulation of protein binding / peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / Negative regulators of DDX58/IFIH1 signaling / cellular response to glucose stimulus / Downregulation of SMAD2/3:SMAD4 transcriptional activity / phosphoprotein binding / SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription / synapse organization / negative regulation of transforming growth factor beta receptor signaling pathway / regulation of protein phosphorylation / lung development / regulation of protein stability / tau protein binding / neuron differentiation / negative regulation of protein catabolic process / negative regulation of ERK1 and ERK2 cascade / ISG15 antiviral mechanism / beta-catenin binding / disordered domain specific binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
![]() | Macias, M.J. / Aragon, E. / Goerner, N. / Zaromytidou, A. / Xi, Q. / Escobedo, A. / Massague, J. | ||||||
![]() | ![]() Title: A Smad action turnover switch operated by WW domain readers of a phosphoserine code. Authors: Aragon, E. / Goerner, N. / Zaromytidou, A.I. / Xi, Q. / Escobedo, A. / Massague, J. / Macias, M.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 275.8 KB | Display | ![]() |
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PDB format | ![]() | 237.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 404.4 KB | Display | ![]() |
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Full document | ![]() | 557.9 KB | Display | |
Data in XML | ![]() | 27.1 KB | Display | |
Data in CIF | ![]() | 41.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2lajC ![]() 2lawC ![]() 2laxC ![]() 2layC ![]() 2lazC ![]() 2lb0C ![]() 2lb1C ![]() 2lb2C C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 4231.692 Da / Num. of mol.: 1 / Fragment: residues 6-41 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 886.882 Da / Num. of mol.: 1 / Fragment: residues 176-183 / Source method: obtained synthetically / Source: (synth.) ![]() |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR Details: Structure of the first domain of human PIN1 in complex with a human Smad3 derived peptide( resi 173-186). | ||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
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Sample conditions | Ionic strength: 0.420 / pH: 7 / Pressure: ambient / Temperature: 285 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||
NMR constraints | NOE constraints total: 557 / NOE intraresidue total count: 0 / NOE long range total count: 215 / NOE medium range total count: 56 / NOE sequential total count: 167 / Hydrogen bond constraints total count: 10 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with acceptable covalent geometry Conformers calculated total number: 300 / Conformers submitted total number: 20 |