regulation of protein deubiquitination / ubiquitin-like protein transferase activity / negative regulation of defense response to virus / protein K29-linked ubiquitination / cellular response to tumor cell / : / T cell anergy / positive regulation of T cell anergy / cellular response to oxidised low-density lipoprotein particle stimulus / negative regulation of cell division ...regulation of protein deubiquitination / ubiquitin-like protein transferase activity / negative regulation of defense response to virus / protein K29-linked ubiquitination / cellular response to tumor cell / : / T cell anergy / positive regulation of T cell anergy / cellular response to oxidised low-density lipoprotein particle stimulus / negative regulation of cell division / regulation of necroptotic process / CD4-positive, alpha-beta T cell proliferation / CXCR chemokine receptor binding / HECT-type E3 ubiquitin transferase / negative regulation of CD4-positive, alpha-beta T cell proliferation / negative regulation of JNK cascade / arrestin family protein binding / enzyme inhibitor activity / Regulation of FOXO transcriptional activity by acetylation / regulation of hematopoietic stem cell differentiation / negative regulation of type I interferon production / protein monoubiquitination / positive regulation of receptor catabolic process / negative regulation of NF-kappaB transcription factor activity / ubiquitin-like protein ligase binding / protein K63-linked ubiquitination / The NLRP3 inflammasome / ligase activity / platelet-derived growth factor receptor signaling pathway / protein K48-linked ubiquitination / ribonucleoprotein complex binding / Purinergic signaling in leishmaniasis infection / protein autoubiquitination / response to glucose / response to mechanical stimulus / keratinocyte differentiation / Downregulation of ERBB4 signaling / Activated NOTCH1 Transmits Signal to the Nucleus / response to progesterone / Negative regulators of DDX58/IFIH1 signaling / regulation of cell growth / Degradation of GLI1 by the proteasome / Hedgehog 'on' state / response to hydrogen peroxide / NOD1/2 Signaling Pathway / receptor internalization / Regulation of necroptotic cell death / Cytoprotection by HMOX1 / response to calcium ion / protein import into nucleus / positive regulation of protein catabolic process / ubiquitin-protein transferase activity / SARS-CoV-1 activates/modulates innate immune responses / ubiquitin protein ligase activity / protein transport / Antigen processing: Ubiquitination & Proteasome degradation / response to estradiol / regulation of cell population proliferation / RUNX1 regulates transcription of genes involved in differentiation of HSCs / cell cortex / ubiquitin-dependent protein catabolic process / cytoplasmic vesicle / early endosome membrane / proteasome-mediated ubiquitin-dependent protein catabolic process / defense response to virus / protein ubiquitination / response to xenobiotic stimulus / inflammatory response / cell cycle / symbiont entry into host cell / positive regulation of apoptotic process / intracellular membrane-bounded organelle / innate immune response / apoptotic process / ubiquitin protein ligase binding / negative regulation of apoptotic process / negative regulation of transcription by RNA polymerase II / protein-containing complex / extracellular exosome / nucleoplasm / membrane / plasma membrane / cytosol / cytoplasm Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 10 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 1.84 Å3/Da / Density % sol: 33.23 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 1.8 M ammonium sulfate, 0.2 M sodium acetate, 0.1 M sodium cacodylate pH 5.5, vapor diffusion, sitting drop, temperature 293K
Resolution: 2.03→30 Å / Cor.coef. Fo:Fc: 0.936 / Cor.coef. Fo:Fc free: 0.894 / WRfactor Rfree: 0.2734 / WRfactor Rwork: 0.2257 / Occupancy max: 1 / Occupancy min: 0.5 / FOM work R set: 0.7465 / SU B: 14.984 / SU ML: 0.188 / SU R Cruickshank DPI: 0.2659 / SU Rfree: 0.2292 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.266 / ESU R Free: 0.229 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: DIFFRACTION IMAGES SHOWED MUTLIPLE PATTERNS AND ICE RINGS. FOR INTEGRATION, DIFFRACTION IMAGES WERE SEPARATED INTO RANGES (PHI 0 THROUGH 63 DEGREES, 63 THROUGH 180 DEGREES, RESPECTIVELY). ...Details: DIFFRACTION IMAGES SHOWED MUTLIPLE PATTERNS AND ICE RINGS. FOR INTEGRATION, DIFFRACTION IMAGES WERE SEPARATED INTO RANGES (PHI 0 THROUGH 63 DEGREES, 63 THROUGH 180 DEGREES, RESPECTIVELY). MOSAICITY AND CRYSTAL-TO-DETECTOR DISTANCE WERE CONSTRAINED DURING INTEGRATION. NO-MERGE-ORIGINAL-INDEX REFLECTIONS FROM SCALEPACK WERE MERGED WITH AIMLESS (ONLYMERGE). ARP/WARP WAS USED FOR AUTOMATED MODEL BUILDING AND MAP IMPROVEMENT. PARROT WAS USED FOR PHASE IMPROVEMENT. RESTRAINTS FOR THE C-TERMINAL AMIDE PROTECTION OF THE PEPTIDE LIGAND WERE PREPARED WITH JLIGAND. COOT WAS USED FOR INTERACTIVE MODEL BUILDING. MODEL GEOMETRY WAS EVALUATED WITH MOLPROBITY.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.2975
943
13.8 %
THIN SHELLS (SFTOOLS)
Rwork
0.2389
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obs
0.2463
6841
99.66 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
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