+Open data
-Basic information
Entry | Database: PDB / ID: 1qcv | ||||||
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Title | RUBREDOXIN VARIANT (PFRD-XC4) FOLDS WITHOUT IRON | ||||||
Components | PROTEIN (RUBREDOXIN VARIANT PFRD-XC4) | ||||||
Keywords | ELECTRON TRANSPORT / HYPERTHERMOPHILE / RUBREDOXIN | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Pyrococcus furiosus (archaea) | ||||||
Method | SOLUTION NMR / distance geometry | ||||||
Authors | Strop, P. / Mayo, S.L. | ||||||
Citation | Journal: Biochemistry / Year: 2000 Title: Contribution of surface salt bridges to protein stability. Authors: Strop, P. / Mayo, S.L. #1: Journal: J.Am.Chem.Soc. / Year: 1999 Title: Rubredoxin Variant Folds without Iron Authors: Strop, P. / Mayo, S.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1qcv.cif.gz | 448.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1qcv.ent.gz | 391.8 KB | Display | PDB format |
PDBx/mmJSON format | 1qcv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qc/1qcv ftp://data.pdbj.org/pub/pdb/validation_reports/qc/1qcv | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 5874.404 Da / Num. of mol.: 1 / Mutation: C5L C8T C38A C41T Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pyrococcus furiosus (archaea) / Production host: Escherichia coli (E. coli) / References: UniProt: P24297 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: DQF-COSY NOESY TOCSY |
-Sample preparation
Details | Contents: 1.5MM PFRD-XC4 ACETATE-D3 PH 6.3; 200MM NACL; 90/10 H2O/D2O |
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Sample conditions | Ionic strength: 225mM / pH: 6.3 / Pressure: 1 atm / Temperature: 298.0 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITYPLUS / Manufacturer: Varian / Model: UNITYPLUS / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: distance geometry / Software ordinal: 1 | ||||||||||||||||
NMR ensemble | Conformer selection criteria: STRUCTURES WITH THE LEAST RESTRAINT VIOLATIONS & LOWEST ENERGY Conformers calculated total number: 40 / Conformers submitted total number: 29 |