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Open data
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Basic information
Entry | Database: PDB / ID: 2ins | ||||||
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Title | THE STRUCTURE OF DES-PHE B1 BOVINE INSULIN | ||||||
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![]() | HORMONE | ||||||
Function / homology | ![]() estradiol secretion / negative regulation of lactation / positive regulation of blood circulation / glucose import in response to insulin stimulus / positive regulation of cell maturation / positive regulation of lactation / response to L-arginine / positive regulation of mammary gland epithelial cell proliferation / negative regulation of appetite / response to butyrate ...estradiol secretion / negative regulation of lactation / positive regulation of blood circulation / glucose import in response to insulin stimulus / positive regulation of cell maturation / positive regulation of lactation / response to L-arginine / positive regulation of mammary gland epithelial cell proliferation / negative regulation of appetite / response to butyrate / feeding behavior / response to growth hormone / response to food / positive regulation of Rho protein signal transduction / positive regulation of peptide hormone secretion / protein secretion / negative regulation of lipid catabolic process / response to glucose / response to nutrient levels / positive regulation of protein secretion / insulin receptor binding / positive regulation of insulin secretion / hormone activity / glucose metabolic process / glucose homeostasis / response to heat / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of gene expression / negative regulation of apoptotic process / extracellular space / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Smith, G.D. / Duax, W.L. / Dodson, E.J. / Dodson, G.G. / Degraaf, R.A.G. / Reynolds, C.D. | ||||||
![]() | Journal: Acta Crystallogr.,Sect.B / Year: 1982 Title: The Structure of Des-Phe B1 Bovine Insulin Authors: Smith, G.D. / Duax, W.L. / Dodson, E.J. / Dodson, G.G. / Degraaf, R.A.G. / Reynolds, C.D. #1: ![]() Title: A Comparative Assessment of the Zinc-Protein Coordination in 2Zn-Insulin as Determined by X-Ray Absorption Fine Structure (Exafs) and X-Ray Crystallography Authors: Bordas, J. / Dodson, G.G. / Grewe, H. / Koch, M.H.J. / Krebs, B. / Randall, J. #2: ![]() Title: Structural Relationships in the Two-Zinc Insulin Hexamer Authors: Dodson, E.J. / Dodson, G.G. / Hodgkin, D.C. / Reynolds, C.D. #3: ![]() Title: Experience with Fast Fourier Least Squares in the Refinement of the Crystal Structure of Rhombohedral 2-Zinc Insulin at 1.5 Angstroms Resolution Authors: Isaacs, N.W. / Agarwal, R.C. #4: ![]() Title: Rhombohedral Insulin Crystal Transformation Authors: Bentley, G. / Dodson, G. / Lewitova, A. #5: ![]() Title: A Method for Fitting Satisfactory Models to Sets of Atomic Positions in Protein Structure Refinements Authors: Dodson, E.J. / Isaacs, N.W. / Rollett, J.S. #8: ![]() Title: Insulin. The Structure in the Crystal and its Reflection in Chemistry and Biology Authors: Blundell, T. / Dodson, G. / Hodgkin, D. / Mercola, D. #9: ![]() Title: The Crystal Structure of Rhombohedral 2 Zinc Insulin Authors: Blundell, T.L. / Cutfield, J.F. / Dodson, E.J. / Dodson, G.G. / Hodgkin, D.C. / Mercola, D.A. #10: ![]() Title: Atomic Positions in Rhombohedral 2-Zinc Insulin Crystals Authors: Blundell, T.L. / Cutfield, J.F. / Cutfield, S.M. / Dodson, E.J. / Dodson, G.G. / Hodgkin, D.C. / Mercola, D.A. / Vijayan, M. #11: ![]() Title: X-Ray Analysis and the Structure of Insulin Authors: Blundell, T.L. / Dodson, G.G. / Dodson, E. / Hodgkin, D.C. / Vijayan, M. #12: ![]() Title: X-Ray Diffraction Data on Some Crystalline Varieties of Insulin Authors: Baker, E.N. / Dodson, G. #13: ![]() Title: Structure of Rhombohedral 2 Zinc Insulin Crystals Authors: Adams, M.J. / Blundell, T.L. / Dodson, E.J. / Dodson, G.G. / Vijayan, M. / Baker, E.N. / Harding, M.M. / Hodgkin, D.C. / Rimmer, B. / Sheat, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 35.7 KB | Display | ![]() |
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PDB format | ![]() | 27.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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5 | ![]()
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Unit cell |
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Atom site foot note | 1: THE QUASI-TWO-FOLD SYMMETRY BREAKS DOWN MOST SERIOUSLY AT RESIDUES GLY A 1 TO GLN A 5 AND GLY C 1 TO GLN C 5 HIS B 5 AND HIS D 5 PHE B 25 AND PHE D 25 2: THE FOLLOWING RESIDUES ARE DISORDERED - ARG B 22, LYS D 29. 3: SEE REMARK 8. | |||||||||||||||
Components on special symmetry positions |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.88, -0.48, 0.02), Details | THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT OF INSULIN CONSISTS OF TWO INSULIN MOLECULES EACH CONSISTING OF TWO CHAINS. THIS ENTRY PRESENTS COORDINATES FOR MOLECULES I (CHAIN INDICATORS A AND B) AND II (CHAIN INDICATORS C AND D). THE QUASI-TWO-FOLD AXIS THAT TRANSFORMS MOLECULE I INTO MOLECULE II IS GIVEN IN THE MTRIX RECORDS BELOW. APPLYING THE THREE-FOLD CRYSTALLOGRAPHIC AXIS YIELDS A HEXAMER AROUND THE AXIS. THERE ARE TWO ZINC IONS SITUATED ON THIS THREE-FOLD AXIS. COORDINATES FOR THE ZINC IONS AND SOME WATER MOLECULES ARE INCLUDED BELOW WITH A BLANK CHAIN INDICATOR. | |
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Components
#1: Protein/peptide | Mass: 2339.645 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Protein/peptide | Mass: 3256.753 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.95 Å3/Da / Density % sol: 36.8 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 6.5 / Method: batch method | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.5 Å / Num. obs: 2722 |
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Processing
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Refinement | Highest resolution: 2.5 Å Details: THE FOLLOWING RESIDUES ARE DISORDERED - ARG B 22, LYS D 29. THE MODEL OF THE WATER STRUCTURE OBTAINED FROM THE REFINEMENT OF 2 ZN PORCINE INSULIN AT 1.5 ANGSTROMS RESOLUTION WAS USED ...Details: THE FOLLOWING RESIDUES ARE DISORDERED - ARG B 22, LYS D 29. THE MODEL OF THE WATER STRUCTURE OBTAINED FROM THE REFINEMENT OF 2 ZN PORCINE INSULIN AT 1.5 ANGSTROMS RESOLUTION WAS USED THROUGHOUT THE DES-PHE B1 INSULIN REFINEMENT.
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Refinement step | Cycle: LAST / Highest resolution: 2.5 Å
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Refine LS restraints |
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Refinement | *PLUS Rfactor obs: 0.18 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: o_bond_d / Dev ideal: 0.02 |