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- PDB-2law: Structure of the second WW domain from human YAP in complex with ... -
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Basic information
Entry | Database: PDB / ID: 2law | ||||||
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Title | Structure of the second WW domain from human YAP in complex with a human Smad1 derived peptide | ||||||
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![]() | SIGNALING PROTEIN/TRANSCRIPTION / YAP / SMAD1 / CDK / signal transduction / SIGNALING PROTEIN-TRANSCRIPTION complex | ||||||
Function / homology | ![]() mesodermal cell fate commitment / homomeric SMAD protein complex / osteoblast fate commitment / enterocyte differentiation / regulation of keratinocyte proliferation / SMAD protein complex / RUNX2 regulates bone development / co-SMAD binding / heteromeric SMAD protein complex / regulation of metanephric nephron tubule epithelial cell differentiation ...mesodermal cell fate commitment / homomeric SMAD protein complex / osteoblast fate commitment / enterocyte differentiation / regulation of keratinocyte proliferation / SMAD protein complex / RUNX2 regulates bone development / co-SMAD binding / heteromeric SMAD protein complex / regulation of metanephric nephron tubule epithelial cell differentiation / negative regulation of muscle cell differentiation / cardiac muscle tissue regeneration / positive regulation of cartilage development / glandular epithelial cell differentiation / TEAD-YAP complex / primary miRNA binding / DEAD/H-box RNA helicase binding / lateral mesoderm development / RUNX3 regulates YAP1-mediated transcription / bud elongation involved in lung branching / polarized epithelial cell differentiation / notochord development / negative regulation of cilium assembly / lung epithelial cell differentiation / gamete generation / hindbrain development / cardiac conduction system development / YAP1- and WWTR1 (TAZ)-stimulated gene expression / heart process / trophectodermal cell differentiation / primary miRNA processing / paraxial mesoderm development / regulation of stem cell proliferation / hippo signaling / tissue homeostasis / EGR2 and SOX10-mediated initiation of Schwann cell myelination / Signaling by BMP / intestinal epithelial cell development / negative regulation of epithelial cell apoptotic process / embryonic pattern specification / SMAD protein signal transduction / Formation of axial mesoderm / negative regulation of stem cell differentiation / embryonic heart tube morphogenesis / female germ cell nucleus / I-SMAD binding / proline-rich region binding / Signaling by Hippo / cartilage development / Cardiogenesis / nuclear inner membrane / cardiac muscle cell proliferation / ureteric bud development / negative regulation of epithelial cell differentiation / midbrain development / organ growth / homeostatic process / positive regulation of stem cell population maintenance / interleukin-6-mediated signaling pathway / positive regulation of Notch signaling pathway / negative regulation of fat cell differentiation / positive regulation of sprouting angiogenesis / RUNX2 regulates osteoblast differentiation / Zygotic genome activation (ZGA) / somatic stem cell population maintenance / cellular response to organic cyclic compound / regulation of neurogenesis / anatomical structure morphogenesis / canonical Wnt signaling pathway / vasculogenesis / BMP signaling pathway / positive regulation of osteoblast differentiation / cellular response to retinoic acid / extrinsic apoptotic signaling pathway / Nuclear signaling by ERBB4 / keratinocyte differentiation / positive regulation of cardiac muscle cell proliferation / ossification / transforming growth factor beta receptor signaling pathway / epithelial cell proliferation / positive regulation of epithelial cell proliferation / response to progesterone / negative regulation of extrinsic apoptotic signaling pathway / wound healing / bone development / cell morphogenesis / cellular response to gamma radiation / positive regulation of miRNA transcription / positive regulation of protein localization to nucleus / transcription corepressor activity / MAPK cascade / cell-cell junction / positive regulation of canonical Wnt signaling pathway / cell junction / RUNX1 regulates transcription of genes involved in differentiation of HSCs / positive regulation of cell growth / protein-containing complex assembly / DNA-binding transcription activator activity, RNA polymerase II-specific / DNA-binding transcription factor binding / transcription by RNA polymerase II Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
![]() | Macias, M.J. / Aragon, E. / Goerner, N. / Zaromytidou, A. / Xi, Q. / Escobedo, A. / Massague, J. | ||||||
![]() | ![]() Title: A Smad action turnover switch operated by WW domain readers of a phosphoserine code. Authors: Aragon, E. / Goerner, N. / Zaromytidou, A.I. / Xi, Q. / Escobedo, A. / Massague, J. / Macias, M.J. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 285.5 KB | Display | ![]() |
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PDB format | ![]() | 236.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 405.2 KB | Display | ![]() |
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Full document | ![]() | 626.3 KB | Display | |
Data in XML | ![]() | 21.6 KB | Display | |
Data in CIF | ![]() | 35.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2lajC ![]() 2laxC ![]() 2layC ![]() 2lazC ![]() 2lb0C ![]() 2lb1C ![]() 2lb2C ![]() 2lb3C C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 4367.827 Da / Num. of mol.: 1 / Fragment: second WW domain, residues 230-263 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 1293.419 Da / Num. of mol.: 1 / Fragment: residues 222-233 / Source method: obtained synthetically / Source: (synth.) ![]() |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR Details: Structure of the second WW domain from human YAP (res 230 -263) in complex with a human Smad1 (res 221-233) derived peptide | ||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
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Sample conditions | Ionic strength: 0.420 / pH: 7 / Pressure: ambient / Temperature: 285 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||
NMR constraints | NOE constraints total: 629 / NOE intraresidue total count: 0 / NOE long range total count: 234 / NOE medium range total count: 78 / NOE sequential total count: 158 / Hydrogen bond constraints total count: 10 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with acceptable covalent geometry Conformers calculated total number: 300 / Conformers submitted total number: 20 |