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- PDB-2e4i: Human Telomeric DNA mixed-parallel/antiparallel quadruplex under ... -
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Basic information
Entry | Database: PDB / ID: 2e4i | ||||||||||||||||||
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Title | Human Telomeric DNA mixed-parallel/antiparallel quadruplex under Physiological Ionic Conditions Stabilized by Proper Incorporation of 8-Bromoguanosines | ||||||||||||||||||
![]() | DNA (5'-D(*![]() DNA / telomere / quadruplex / mixed-parallel/antiparallel | Function / homology | DNA / DNA (> 10) | ![]() Method | SOLUTION NMR / simulated annealing | ![]() Matsugami, A. / Xu, Y. / Noguchi, Y. / Sugiyama, H. / Katahira, M. | ![]() ![]() Title: Structure of a human telomeric DNA sequence stabilized by 8-bromoguanosine substitutions, as determined by NMR in a K+ solution Authors: Matsugami, A. / Xu, Y. / Noguchi, Y. / Sugiyama, H. / Katahira, M. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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PDBx/mmCIF format | ![]() | 21.7 KB | Display | ![]() |
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PDB format | ![]() | 15.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 251.6 KB | Display | ![]() |
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Full document | ![]() | 251.7 KB | Display | |
Data in XML | ![]() | 2.3 KB | Display | |
Data in CIF | ![]() | 2.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: DNA chain | Mass: 7377.977 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: Human Telomeric DNA |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details |
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Sample conditions | Ionic strength: 110mM Potassium Ion / pH: 6.3 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: the structures are based on a total of 752 restraints, 582 are NOE-derived distance constraints, 110 dihedral angle restraints,48 distance restraints from hydrogen bonds, 12 planarity ...Details: the structures are based on a total of 752 restraints, 582 are NOE-derived distance constraints, 110 dihedral angle restraints,48 distance restraints from hydrogen bonds, 12 planarity restraints for tetrad planes. | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 1 |