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- PDB-2lax: Structure of first WW domain of human YAP in complex with a human... -

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Basic information

Entry
Database: PDB / ID: 2lax
TitleStructure of first WW domain of human YAP in complex with a human Smad1 doubly-phosphorilated derived peptide.
Components
  • Mothers against decapentaplegic homolog 1
  • Yorkie homolog
KeywordsSIGNALING PROTEIN/TRANSCRIPTION / YAP / SMAD / CDK / signal transduction / SIGNALING PROTEIN-TRANSCRIPTION complex
Function / homology
Function and homology information


mesodermal cell fate commitment / homomeric SMAD protein complex / osteoblast fate commitment / enterocyte differentiation / SMAD protein complex / regulation of keratinocyte proliferation / RUNX2 regulates bone development / heteromeric SMAD protein complex / co-SMAD binding / regulation of metanephric nephron tubule epithelial cell differentiation ...mesodermal cell fate commitment / homomeric SMAD protein complex / osteoblast fate commitment / enterocyte differentiation / SMAD protein complex / regulation of keratinocyte proliferation / RUNX2 regulates bone development / heteromeric SMAD protein complex / co-SMAD binding / regulation of metanephric nephron tubule epithelial cell differentiation / negative regulation of muscle cell differentiation / cardiac muscle tissue regeneration / positive regulation of cartilage development / glandular epithelial cell differentiation / TEAD-YAP complex / lateral mesoderm development / DEAD/H-box RNA helicase binding / polarized epithelial cell differentiation / bud elongation involved in lung branching / primary miRNA binding / RUNX3 regulates YAP1-mediated transcription / notochord development / negative regulation of cilium assembly / gamete generation / hindbrain development / lung epithelial cell differentiation / cardiac conduction system development / YAP1- and WWTR1 (TAZ)-stimulated gene expression / heart process / trophectodermal cell differentiation / primary miRNA processing / paraxial mesoderm development / hippo signaling / regulation of stem cell proliferation / EGR2 and SOX10-mediated initiation of Schwann cell myelination / negative regulation of epithelial cell apoptotic process / intestinal epithelial cell development / tissue homeostasis / SMAD protein signal transduction / Signaling by BMP / embryonic pattern specification / Formation of axial mesoderm / negative regulation of stem cell differentiation / embryonic heart tube morphogenesis / female germ cell nucleus / I-SMAD binding / proline-rich region binding / Signaling by Hippo / Differentiation of Keratinocytes in Interfollicular Epidermis in Mammalian Skin / nuclear inner membrane / Cardiogenesis / cartilage development / positive regulation of dendrite development / ureteric bud development / cardiac muscle cell proliferation / organ growth / negative regulation of epithelial cell differentiation / interleukin-6-mediated signaling pathway / positive regulation of Notch signaling pathway / negative regulation of fat cell differentiation / midbrain development / positive regulation of sprouting angiogenesis / RUNX2 regulates osteoblast differentiation / positive regulation of stem cell population maintenance / Zygotic genome activation (ZGA) / : / regulation of neurogenesis / somatic stem cell population maintenance / anatomical structure morphogenesis / bicellular tight junction / canonical Wnt signaling pathway / BMP signaling pathway / positive regulation of osteoblast differentiation / vasculogenesis / Nuclear signaling by ERBB4 / Transcriptional regulation of brown and beige adipocyte differentiation by EBF2 / keratinocyte differentiation / positive regulation of cardiac muscle cell proliferation / cellular response to retinoic acid / extrinsic apoptotic signaling pathway / transforming growth factor beta receptor signaling pathway / ossification / male germ cell nucleus / response to progesterone / positive regulation of epithelial cell proliferation / epithelial cell proliferation / Turbulent (oscillatory, disturbed) flow shear stress activates signaling by PIEZO1 and integrins in endothelial cells / negative regulation of extrinsic apoptotic signaling pathway / stem cell differentiation / transcription coregulator activity / wound healing / cellular response to gamma radiation / cell morphogenesis / bone development / positive regulation of miRNA transcription / positive regulation of protein localization to nucleus / transcription corepressor activity / osteoblast differentiation / cell junction / positive regulation of canonical Wnt signaling pathway
Similarity search - Function
: / Omega loop, TEAD interating region 3 / : / MAD homology, MH1 / Dwarfin / SMAD MH1 domain superfamily / MAD homology domain 1 (MH1) profile. / SMAD domain, Dwarfin-type / MH2 domain / MAD homology domain 2 (MH2) profile. ...: / Omega loop, TEAD interating region 3 / : / MAD homology, MH1 / Dwarfin / SMAD MH1 domain superfamily / MAD homology domain 1 (MH1) profile. / SMAD domain, Dwarfin-type / MH2 domain / MAD homology domain 2 (MH2) profile. / Domain B in dwarfin family proteins / MAD homology 1, Dwarfin-type / MH1 domain / Domain A in dwarfin family proteins / SMAD-like domain superfamily / SMAD/FHA domain superfamily / WW domain / WW/rsp5/WWP domain signature. / WW domain superfamily / WW/rsp5/WWP domain profile. / Domain with 2 conserved Trp (W) residues / WW domain
Similarity search - Domain/homology
Transcriptional coactivator YAP1 / Mothers against decapentaplegic homolog 1
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodSOLUTION NMR / simulated annealing
Model detailslowest energy, model 1
AuthorsMacias, M.J. / Aragon, E. / Goerner, N. / Zaromytidou, A. / Xi, Q. / Escobedo, A. / Massague, J.
CitationJournal: Genes Dev. / Year: 2011
Title: A Smad action turnover switch operated by WW domain readers of a phosphoserine code.
Authors: Aragon, E. / Goerner, N. / Zaromytidou, A.I. / Xi, Q. / Escobedo, A. / Massague, J. / Macias, M.J.
History
DepositionMar 22, 2011Deposition site: BMRB / Processing site: RCSB
Revision 1.0Jul 6, 2011Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Version format compliance
Revision 1.2Nov 27, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature / pdbx_nmr_software / struct_conn / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_nmr_software.name / _struct_conn.pdbx_leaving_atom_flag / _struct_ref_seq_dif.details

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Yorkie homolog
B: Mothers against decapentaplegic homolog 1


Theoretical massNumber of molelcules
Total (without water)5,6772
Polymers5,6772
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 300structures with acceptable covalent geometry
RepresentativeModel #1lowest energy

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Components

#1: Protein/peptide Yorkie homolog / 65 kDa Yes-associated protein / YAP65


Mass: 4540.034 Da / Num. of mol.: 1 / Fragment: first WW domain, residues 170-205
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: YAP1, YAP65 / Production host: Escherichia coli (E. coli) / References: UniProt: P46937
#2: Protein/peptide Mothers against decapentaplegic homolog 1 / MAD homolog 1 / Mothers against DPP homolog 1 / JV4-1 / Mad-related protein 1 / SMAD family member ...MAD homolog 1 / Mothers against DPP homolog 1 / JV4-1 / Mad-related protein 1 / SMAD family member 1 / SMAD 1 / Smad1 / hSMAD1 / Transforming growth factor-beta-signaling protein 1 / BSP-1


Mass: 1136.965 Da / Num. of mol.: 1 / Fragment: residues 201-209 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: Q15797
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
Details: Structure of first WW domain of human YAP in complex with a human Smad1 doubly-phosphorilated derived peptide.
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1112D 1H-1H NOESY
1212D 1H-1H TOCSY
1333D CBCA(CO)NH
1433D HN(CA)CB
1522D 1H-15N HSQC

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Sample preparation

Details
Solution-IDContentsSolvent system
11 mM NEDD4LWW3, 3 mM SMAD3, 20 mM sodium phosphate, 100 mM sodium chloride, 2 mM sodium azide, 90% H2O/10% D2O90% H2O/10% D2O
21 mM [U-100% 15N] NEDD4LWW3, 3 mM SMAD3, 20 mM sodium phosphate, 100 mM sodium chloride, 2 mM sodium azide, 90% H2O/10% D2O90% H2O/10% D2O
31 mM [U-100% 13C; U-100% 15N] NEDD4LWW3, 3 mM SMAD3, 20 mM sodium phosphate, 100 mM sodium chloride, 2 mM sodium azide, 90% H2O/10% D2O90% H2O/10% D2O
Sample
Conc. (mg/ml)ComponentIsotopic labelingSolution-ID
1 mMNEDD4LWW3-11
3 mMSMAD3-21
20 mMsodium phosphate-31
100 mMsodium chloride-41
2 mMsodium azide-51
1 mMNEDD4LWW3-6[U-100% 15N]2
3 mMSMAD3-72
20 mMsodium phosphate-82
100 mMsodium chloride-92
2 mMsodium azide-102
1 mMNEDD4LWW3-11[U-100% 13C; U-100% 15N]3
3 mMSMAD3-123
20 mMsodium phosphate-133
100 mMsodium chloride-143
2 mMsodium azide-153
Sample conditionsIonic strength: 0.42 / pH: 7 / Pressure: ambient / Temperature: 285 K

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NMR measurement

NMR spectrometerType: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz

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Processing

NMR software
NameVersionDeveloperClassification
CNS1.3Brunger, Adams, Clore, Gros, Nilges and Readstructure solution
XEASYBartels et al.chemical shift assignment
TopSpinBruker Biospincollection
NMRPipeDelaglio, Grzesiek, Vuister, Zhu, Pfeifer and Baxprocessing
CNSrefinement
RefinementMethod: simulated annealing / Software ordinal: 1
NMR constraintsNOE constraints total: 600 / NOE intraresidue total count: 0 / NOE long range total count: 248 / NOE medium range total count: 81 / NOE sequential total count: 170 / Hydrogen bond constraints total count: 10
NMR representativeSelection criteria: lowest energy
NMR ensembleConformer selection criteria: structures with acceptable covalent geometry
Conformers calculated total number: 300 / Conformers submitted total number: 20

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