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- PDB-1mcp: PHOSPHOCHOLINE BINDING IMMUNOGLOBULIN FAB MC/PC603. AN X-RAY DIFF... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1mcp | |||||||||
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Title | PHOSPHOCHOLINE BINDING IMMUNOGLOBULIN FAB MC/PC603. AN X-RAY DIFFRACTION STUDY AT 2.7 ANGSTROMS | |||||||||
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![]() | IMMUNOGLOBULIN | |||||||||
Function / homology | ![]() immunoglobulin complex / immunoglobulin mediated immune response / antigen binding / blood microparticle Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() | |||||||||
![]() | Satow, Y. / Cohen, G.H. / Padlan, E.A. / Davies, D.R. | |||||||||
![]() | ![]() Title: Phosphocholine binding immunoglobulin Fab McPC603. An X-ray diffraction study at 2.7 A. Authors: Satow, Y. / Cohen, G.H. / Padlan, E.A. / Davies, D.R. #1: ![]() Title: Kappa Chain Structure from a Crystallized Murine Fab(Prime). Role of Joining Segment in Hapten Binding Authors: Rudikoff, S. / Satow, Y. / Padlan, E. / Davies, D. / Potter, M. #2: ![]() Title: Immunoglobulin Structures at High Resolution Authors: Davies, D.R. / Padlan, E.A. / Segal, D.M. #3: ![]() Year: 1974 Title: The Three-Dimensional Structure of the Antigen Binding Site of Mc/Pc 603 Protein Authors: Padlan, E.A. / Segal, D.M. / Cohen, G.H. / Davies, D.R. / Rudikoff, S. / Potter, M. #4: ![]() Title: The Three-Dimensional Structure of a Phosphorylcholine-Binding Mouse Immunoglobulin Fab and the Nature of the Antigen Binding Site Authors: Segal, D.M. / Padlan, E.A. / Cohen, G.H. / Rudikoff, S. / Potter, M. / Davies, D.R. #5: ![]() Title: Structure at 4.5 Angstroms Resolution of a Phosphorylcholine-Binding Fab Authors: Padlan, E.A. / Segal, D.M. / Spande, T.F. / Davies, D.R. / Rudikoff, S. / Potter, M. #6: ![]() Title: Crystals of Phosphorylcholine-Binding Fab-Fragments from Mouse Myeloma Proteins. Preparation and X-Ray Analysis Authors: Rudikoff, S. / Potter, M. / Segal, D.M. / Padlan, E.A. / Davies, D.R. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 98.3 KB | Display | ![]() |
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PDB format | ![]() | 77.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 442.8 KB | Display | ![]() |
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Full document | ![]() | 499.9 KB | Display | |
Data in XML | ![]() | 27.6 KB | Display | |
Data in CIF | ![]() | 36.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Atom site foot note | 1: FOR RESIDUES 28-37 AND 162-163 OF THE LIGHT CHAIN AND RESIDUE 202 OF THE HEAVY CHAIN, THE DENSITY WAS POOR. THESE RESIDUES WERE BUILT MAINLY BY STEREOCHEMISTRY. 2: RESIDUES PRO L 8, PRO L 101, PRO L 147, PRO H 143, PRO H 155 ARE CIS PROLINES. |
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Components
#1: Antibody | Mass: 24113.584 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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#2: Antibody | Mass: 24319.266 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
#3: Chemical | ChemComp-SO4 / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.78 Å3/Da / Density % sol: 74.25 % | |||||||||||||||
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Crystal grow | *PLUS pH: 7 / Method: vapor diffusion | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.7 Å / Lowest resolution: 10 Å / Num. obs: 24235 / Num. measured all: 147706 |
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Processing
Refinement | Rfactor Rwork: 0.225 / Highest resolution: 2.7 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement step | Cycle: LAST / Highest resolution: 2.7 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2.7 Å / Lowest resolution: 8 Å / Num. reflection obs: 23737 / Rfactor obs: 0.225 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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