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Yorodumi- PDB-1nld: FAB FRAGMENT OF A NEUTRALIZING ANTIBODY DIRECTED AGAINST AN EPITO... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1nld | ||||||
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Title | FAB FRAGMENT OF A NEUTRALIZING ANTIBODY DIRECTED AGAINST AN EPITOPE OF GP41 FROM HIV-1 | ||||||
Components | (FAB1583) x 2 | ||||||
Keywords | IMMUNOGLOBULIN / FAB FRAGMENT | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta / : Function and homology information | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.9 Å | ||||||
Authors | Davies, C. / Beauchamp, J.C. / Emery, D. / Rawas, A. / Muirhead, H. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 1997 Title: Structure of the Fab fragment from a neutralizing monoclonal antibody directed against an epitope of gp41 from HIV-1. Authors: Davies, C. / Beauchamp, J.C. / Emery, D. / Rawas, A. / Muirhead, H. #1: Journal: Nature / Year: 1989 Title: An Engineered Poliovirus Chimaera Elicits Broadly Reactive HIV-1 Neutralizing Antibodies Authors: Evans, D.J. / Mckeating, J. / Meredith, J.M. / Burke, K.L. / Katrak, K. / John, A. / Ferguson, M. / Minor, P.D. / Weiss, R.A. / Almond, J.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1nld.cif.gz | 87.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1nld.ent.gz | 71.8 KB | Display | PDB format |
PDBx/mmJSON format | 1nld.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nl/1nld ftp://data.pdbj.org/pub/pdb/validation_reports/nl/1nld | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Antibody | Mass: 24186.941 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / Cell line: 1583 HYBRIDOMA / References: PIR: PC4203 |
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#2: Antibody | Mass: 22959.670 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / Cell line: 1583 HYBRIDOMA |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.19 Å3/Da / Density % sol: 46 % | |||||||||||||||
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Crystal | *PLUS | |||||||||||||||
Crystal grow | *PLUS Temperature: 291 K / Method: vapor diffusion, hanging drop / PH range low: 5.5 / PH range high: 5 | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: 1992 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 8958 / % possible obs: 91.6 % / Observed criterion σ(I): 0 / Redundancy: 3.1 % / Rmerge(I) obs: 0.1016 |
Reflection | *PLUS Highest resolution: 2.88 Å / Lowest resolution: 5.24 Å / Num. measured all: 27644 |
-Processing
Software |
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Refinement | Resolution: 2.9→8 Å / σ(F): 0
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Displacement parameters | Biso mean: 16.6 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.9→8 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_deg / Dev ideal: 1.7 |