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Open data
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Basic information
Entry | Database: PDB / ID: 1cem | ||||||
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Title | ENDOGLUCANASE A (CELA) CATALYTIC CORE, RESIDUES 33-395 | ||||||
![]() | CELLULASE CELA (1,4-BETA-D-GLUCAN-GLUCANOHYDROLASE) | ||||||
![]() | ![]() ![]() ![]() ![]() | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Alzari, P.M. | ||||||
![]() | ![]() Title: The crystal structure of endoglucanase CelA, a family 8 glycosyl hydrolase from Clostridium thermocellum. Authors: Alzari, P.M. / Souchon, H. / Dominguez, R. #1: ![]() Title: Crystallization of a Family 8 Cellulase from Clostridium Thermocellum Authors: Souchon, H. / Beguin, P. / Alzari, P.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 86.6 KB | Display | ![]() |
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PDB format | ![]() | 64.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 40345.277 Da / Num. of mol.: 1 / Fragment: CATALYTIC CORE RESIDUES 33-395 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: UniProt: P04955, UniProt: A3DC29*PLUS, ![]() |
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#2: Water | ChemComp-HOH / ![]() |
Compound details | THE COORDINATES REPORTED IN THIS ENTRY ARE THOSE OF THE CATALYTIC CORE, RESIDUES 33 - 395, OBTAINED ...THE COORDINATE |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.07 Å3/Da / Density % sol: 40.58 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal | *PLUS Density % sol: 38 % | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow![]() | *PLUS pH: 7.5 / Method: unknownDetails: macroseeding, Souchon, H., (1996) Proteins: Struct.,Funct., Genet., 25, 134. | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: MARRESEARCH / Detector: IMAGE PLATE |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.65→20 Å / Num. obs: 39653 / % possible obs: 96.9 % / Redundancy: 6.26 % / Rmerge(I) obs: 0.05 |
Reflection | *PLUS Num. obs: 39868 / Num. measured all: 249508 |
Reflection shell | *PLUS % possible obs: 94 % / Rmerge(I) obs: 0.155 |
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Processing
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Refinement | Resolution: 1.65→10 Å
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Displacement parameters | Biso mean: 14.26 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.65→10 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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