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- PDB-1is9: Endoglucanase A from Clostridium thermocellum at atomic resolution -
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Open data
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Basic information
Entry | Database: PDB / ID: 1is9 | ||||||
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Title | Endoglucanase A from Clostridium thermocellum at atomic resolution | ||||||
![]() | endoglucanase A | ||||||
![]() | HYDROLASE / endoglucanase family 8 / Hg derivative | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Schmidt, A. / Gonzalez, A. / Morris, R.J. / Costabel, M. / Alzari, P.M. / Lamzin, V.S. | ||||||
![]() | ![]() Title: Advantages of high-resolution phasing: MAD to atomic resolution. Authors: Schmidt, A. / Gonzalez, A. / Morris, R.J. / Costabel, M. / Alzari, P.M. / Lamzin, V.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 166.7 KB | Display | ![]() |
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PDB format | ![]() | 127.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 373.3 KB | Display | ![]() |
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Full document | ![]() | 380.2 KB | Display | |
Data in XML | ![]() | 9.9 KB | Display | |
Data in CIF | ![]() | 18.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 40345.277 Da / Num. of mol.: 1 / Fragment: residues 1-363 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P04955, UniProt: A3DC29*PLUS, cellulase | ||||
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#2: Chemical | ChemComp-HG / #3: Chemical | ChemComp-CL / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 38.47 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 7.5 / Method: unknownDetails: macroseeding, Souchon, H., (1996) Proteins: Struct.,Funct., Genet., 25, 134. | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Aug 1, 1997 | ||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 1→25 Å / Num. all: 158384 / Num. obs: 158384 / % possible obs: 97 % / Redundancy: 4 % / Biso Wilson estimate: 5.5 Å2 / Rsym value: 0.051 / Net I/σ(I): 14 | ||||||||||||
Reflection shell | Resolution: 1.02→1.03 Å / Redundancy: 4 % / Mean I/σ(I) obs: 3 / Rsym value: 0.35 / % possible all: 91 | ||||||||||||
Reflection | *PLUS Highest resolution: 1.03 Å / Lowest resolution: 30 Å / Num. all: 304029 / % possible obs: 98.2 % / Num. measured all: 1605139 / Rmerge(I) obs: 0.049 | ||||||||||||
Reflection shell | *PLUS % possible obs: 90.9 % / Rmerge(I) obs: 0.355 / Mean I/σ(I) obs: 3.9 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 1.03→25 Å
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Refinement | *PLUS Lowest resolution: 25 Å / Rfactor Rfree: 0.149 / Rfactor Rwork: 0.132 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |