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基本情報
登録情報 | データベース: EMDB / ID: EMD-4169 | |||||||||
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タイトル | N terminal region of dynein tail domains in complex with dynactin filament and BICDR-1 | |||||||||
![]() | Map of the N-terminal half of two dynein tail domains bound to dynactin and BICDR1. This map was generated after particle signal subtraction from the overall map (separate deposition) | |||||||||
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機能・相同性 | ![]() RHOD GTPase cycle / Factors involved in megakaryocyte development and platelet production / Golgi to secretory granule transport / RHOF GTPase cycle / dynactin complex / Regulation of PLK1 Activity at G2/M Transition / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Anchoring of the basal body to the plasma membrane ...RHOD GTPase cycle / Factors involved in megakaryocyte development and platelet production / Golgi to secretory granule transport / RHOF GTPase cycle / dynactin complex / Regulation of PLK1 Activity at G2/M Transition / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / transport along microtubule / WASH complex / F-actin capping protein complex / positive regulation of intracellular transport / dynein light chain binding / negative regulation of filopodium assembly / regulation of metaphase plate congression / dynein heavy chain binding / establishment of spindle localization / axonemal dynein complex / positive regulation of spindle assembly / vesicle transport along microtubule / dynein complex / P-body assembly / COPI-independent Golgi-to-ER retrograde traffic / minus-end-directed microtubule motor activity / barbed-end actin filament capping / dynein light intermediate chain binding / cytoplasmic dynein complex / regulation of cell morphogenesis / regulation of lamellipodium assembly / retrograde axonal transport / nuclear migration / Recruitment of NuMA to mitotic centrosomes / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / MHC class II antigen presentation / COPI-mediated anterograde transport / microtubule motor activity / dynein intermediate chain binding / microtubule-based movement / cytoplasmic microtubule / dynactin binding / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / COPI-mediated anterograde transport / stress granule assembly / Mitotic Prometaphase / cytoplasmic microtubule organization / EML4 and NUDC in mitotic spindle formation / regulation of mitotic spindle organization / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / axon cytoplasm / Recruitment of mitotic centrosome proteins and complexes / cytoskeleton organization / Recruitment of NuMA to mitotic centrosomes / Resolution of Sister Chromatid Cohesion / Anchoring of the basal body to the plasma membrane / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / MHC class II antigen presentation / sarcomere / AURKA Activation by TPX2 / mitotic spindle organization / filopodium / RHO GTPases Activate Formins / cell morphogenesis / small GTPase binding / Aggrephagy / HCMV Early Events / Separation of Sister Chromatids / neuron projection development / Regulation of PLK1 Activity at G2/M Transition / azurophil granule lumen / actin filament binding / positive regulation of cold-induced thermogenesis / actin binding / cell cortex / actin cytoskeleton organization / microtubule / vesicle / cell division / centrosome / Neutrophil degranulation / ATP hydrolysis activity / RNA binding / extracellular exosome / extracellular region / ATP binding / membrane / cytoplasm / cytosol 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() ![]() ![]() ![]() ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.4 Å | |||||||||
![]() | Urnavicius L / Lau CK / Elshenawy MM / Morales-Rios E / Motz C / Yildiz A / Carter AP | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Cryo-EM shows how dynactin recruits two dyneins for faster movement. 著者: Linas Urnavicius / Clinton K Lau / Mohamed M Elshenawy / Edgar Morales-Rios / Carina Motz / Ahmet Yildiz / Andrew P Carter / ![]() ![]() ![]() 要旨: Dynein and its cofactor dynactin form a highly processive microtubule motor in the presence of an activating adaptor, such as BICD2. Different adaptors link dynein and dynactin to distinct cargoes. ...Dynein and its cofactor dynactin form a highly processive microtubule motor in the presence of an activating adaptor, such as BICD2. Different adaptors link dynein and dynactin to distinct cargoes. Here we use electron microscopy and single-molecule studies to show that adaptors can recruit a second dynein to dynactin. Whereas BICD2 is biased towards recruiting a single dynein, the adaptors BICDR1 and HOOK3 predominantly recruit two dyneins. We find that the shift towards a double dynein complex increases both the force and speed of the microtubule motor. Our 3.5 Å resolution cryo-electron microscopy reconstruction of a dynein tail-dynactin-BICDR1 complex reveals how dynactin can act as a scaffold to coordinate two dyneins side-by-side. Our work provides a structural basis for understanding how diverse adaptors recruit different numbers of dyneins and regulate the motile properties of the dynein-dynactin transport machine. | |||||||||
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構造の表示
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構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
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画像 | ![]() | 139 KB | ||
その他 | ![]() ![]() | 1 MB 1.2 MB | ||
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-検証レポート
文書・要旨 | ![]() | 226.6 KB | 表示 | ![]() |
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-関連構造データ
関連構造データ | ![]() 6f1uMC ![]() 4168C ![]() 4170C ![]() 4171C ![]() 4172C ![]() 4177C ![]() 5owoC ![]() 6f1tC ![]() 6f1vC ![]() 6f1yC ![]() 6f1zC ![]() 6f38C ![]() 6f3aC C: 同じ文献を引用 ( M: このマップから作成された原子モデル |
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類似構造データ |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | Map of the N-terminal half of two dynein tail domains bound to dynactin and BICDR1. This map was generated after particle signal subtraction from the overall map (separate deposition) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.34 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-追加マップ: Map of summed signal of dynein heavy chains present in main map
ファイル | emd_4169_additional_1.map | ||||||||||||
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注釈 | Map of summed signal of dynein heavy chains present in main map | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-追加マップ: Map of summed signal of dynein intermediate chains...
ファイル | emd_4169_additional_2.map | ||||||||||||
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注釈 | Map of summed signal of dynein intermediate chains present in main map | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
+全体 : Two dynein tail domains bound to dynactin and BICDR1.
+超分子 #1: Two dynein tail domains bound to dynactin and BICDR1.
+超分子 #2: dynactin filament
+超分子 #3: Cytoplasmic dynein
+超分子 #4: BICDR1
+分子 #1: ARP1 actin related protein 1 homolog A
+分子 #2: Capping protein (Actin filament) muscle Z-line, alpha 1
+分子 #3: F-actin capping protein beta subunit
+分子 #4: Dynactin subunit 2
+分子 #5: Dynactin subunit 2
+分子 #6: Cytoplasmic dynein 1 heavy chain 1
+分子 #7: Cytoplasmic dynein 1 intermediate chain 2
+分子 #8: BICD family-like cargo adapter 1
+分子 #9: ADENOSINE-5'-DIPHOSPHATE
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE / 装置: FEI VITROBOT MARK III |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON II (4k x 4k) 平均電子線量: 52.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
-原子モデル構築 1
精密化 | 空間: REAL 当てはまり具合の基準: Cross-correlation coefficient |
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得られたモデル | ![]() PDB-6f1u: |