+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-30140 | |||||||||
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Title | Mature Phage T7 mismatch | |||||||||
Map data | ||||||||||
Sample |
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Biological species | Escherichia phage T7 (virus) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.6 Å | |||||||||
Authors | Chen WY / Xiao H | |||||||||
Funding support | 1 items
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Citation | Journal: Protein Cell / Year: 2020 Title: Structural changes of a bacteriophage upon DNA packaging and maturation. Authors: Wenyuan Chen / Hao Xiao / Xurong Wang / Shuanglin Song / Zhen Han / Xiaowu Li / Fan Yang / Li Wang / Jingdong Song / Hongrong Liu / Lingpeng Cheng / | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_30140.map.gz | 471.5 MB | EMDB map data format | |
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Header (meta data) | emd-30140-v30.xml emd-30140.xml | 7.5 KB 7.5 KB | Display Display | EMDB header |
Images | emd_30140.png | 58.7 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-30140 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-30140 | HTTPS FTP |
-Validation report
Summary document | emd_30140_validation.pdf.gz | 364.6 KB | Display | EMDB validaton report |
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Full document | emd_30140_full_validation.pdf.gz | 364.2 KB | Display | |
Data in XML | emd_30140_validation.xml.gz | 8.4 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-30140 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-30140 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_30140.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 1.27 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : Escherichia virus T7
Entire | Name: Escherichia virus T7 |
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Components |
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-Supramolecule #1: Escherichia virus T7
Supramolecule | Name: Escherichia virus T7 / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Escherichia phage T7 (virus) |
Recombinant expression | Organism: Escherichia coli BL21 (bacteria) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: NITROGEN |
-Electron microscopy
Microscope | FEI TECNAI ARCTICA |
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Image recording | Film or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 24.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Talos Arctica / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 55212 |
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Initial angle assignment | Type: COMMON LINE |
Final angle assignment | Type: PROJECTION MATCHING |