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- PDB-7boz: N-teminal of mature bacteriophage T7 tail fiber protein gp17 -

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Basic information

Entry
Database: PDB / ID: 7boz
TitleN-teminal of mature bacteriophage T7 tail fiber protein gp17
ComponentsN-teminal of mature bacteriophage T7 tail fiber protein gp17
KeywordsSTRUCTURAL PROTEIN / phage / portal / protein / mature
Function / homology
Function and homology information


virus tail, fiber / adhesion receptor-mediated virion attachment to host cell / viral entry into host cell / virion attachment to host cell / identical protein binding
Similarity search - Function
Tail fibre protein gp37 C terminal / Bacteriophage T7, Gp17, C-terminal / Bacteriophage T7 tail fibre protein / Phage T7 tail fibre protein
Similarity search - Domain/homology
Biological speciesEscherichia phage T7 (bacteriophage)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.8 Å
AuthorsChen, W.Y. / Xiao, H.
Funding support1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)
CitationJournal: Protein Cell / Year: 2020
Title: Structural changes of a bacteriophage upon DNA packaging and maturation.
Authors: Wenyuan Chen / Hao Xiao / Xurong Wang / Shuanglin Song / Zhen Han / Xiaowu Li / Fan Yang / Li Wang / Jingdong Song / Hongrong Liu / Lingpeng Cheng /
History
DepositionMar 20, 2020Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Mar 17, 2021Provider: repository / Type: Initial release

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Assembly

Deposited unit
a: N-teminal of mature bacteriophage T7 tail fiber protein gp17
b: N-teminal of mature bacteriophage T7 tail fiber protein gp17
c: N-teminal of mature bacteriophage T7 tail fiber protein gp17
d: N-teminal of mature bacteriophage T7 tail fiber protein gp17
e: N-teminal of mature bacteriophage T7 tail fiber protein gp17
f: N-teminal of mature bacteriophage T7 tail fiber protein gp17
g: N-teminal of mature bacteriophage T7 tail fiber protein gp17
h: N-teminal of mature bacteriophage T7 tail fiber protein gp17
i: N-teminal of mature bacteriophage T7 tail fiber protein gp17
j: N-teminal of mature bacteriophage T7 tail fiber protein gp17
k: N-teminal of mature bacteriophage T7 tail fiber protein gp17
l: N-teminal of mature bacteriophage T7 tail fiber protein gp17
m: N-teminal of mature bacteriophage T7 tail fiber protein gp17
n: N-teminal of mature bacteriophage T7 tail fiber protein gp17
o: N-teminal of mature bacteriophage T7 tail fiber protein gp17
p: N-teminal of mature bacteriophage T7 tail fiber protein gp17
q: N-teminal of mature bacteriophage T7 tail fiber protein gp17
r: N-teminal of mature bacteriophage T7 tail fiber protein gp17


Theoretical massNumber of molelcules
Total (without water)1,109,55418
Polymers1,109,55418
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area33830 Å2
ΔGint-241 kcal/mol
Surface area130160 Å2

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Components

#1: Protein
N-teminal of mature bacteriophage T7 tail fiber protein gp17 / Gene product 17 / Gp17 / Tail fiber protein gp17


Mass: 61641.875 Da / Num. of mol.: 18
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia phage T7 (bacteriophage) / Gene: 17 / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P03748

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: N-teminal of mature bacteriophage T7 tail fiber protein gp17
Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT
Source (natural)Organism: Escherichia phage T7 (bacteriophage)
Buffer solutionpH: 7
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: NITROGEN

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Electron microscopy imaging

Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company
MicroscopyModel: FEI TECNAI ARCTICA
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 24 e/Å2 / Film or detector model: FEI FALCON II (4k x 4k)

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Processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 55212 / Symmetry type: POINT

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