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- EMDB-11903: Staphylococcus aureus 70S after 50 minutes incubation at 37C -

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Open data

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Basic information

Entry

Database: EMDB / ID: EMD-11903

Title

Staphylococcus aureus 70S after 50 minutes incubation at 37C

Map data

Sample

Complex: 70S
- RNA: x 3 types
- Protein or peptide: x 43 types

Keywords

Ribosome / H68 /

translation /

protein synthesis

Function / homology

Function and homology information

large ribosomal subunit / small ribosomal subunit /

5S rRNA binding /

transferase activity /

tRNA binding /

rRNA binding /

ribosome / structural constituent of ribosome /

translation /

ribonucleoprotein complex ...
Similarity search - Function

Type-1 KH domain profile. /

Ribosomal protein L25, long-form /

Ribosomal protein L25, beta domain /

Ribosomal protein L25, C-terminal /

Ribosomal protein TL5, C-terminal domain /

Ribosomal protein S14, type Z /

Ribosomal protein L16 signature 1. /

: /

Ribosomal protein L6, conserved site /

Ribosomal protein L6 signature 1. ...Type-1 KH domain profile. /

Ribosomal protein L25, long-form /

Ribosomal protein L25, beta domain /

Ribosomal protein L25, C-terminal /

Ribosomal protein TL5, C-terminal domain /

Ribosomal protein S14, type Z /

Ribosomal protein L16 signature 1. /

: /

Ribosomal protein L6, conserved site /

Ribosomal protein L6 signature 1. /

Ribosomal protein L16, conserved site /

Ribosomal protein L16 signature 2. /

Ribosomal protein L17 signature. / Ribosomal L25p family /

Ribosomal protein L25 /

Ribosomal protein S14/S29 /

Ribosomal protein L28/L24 superfamily /

Ribosomal protein L36 signature. /

Ribosomal protein L25/Gln-tRNA synthetase, N-terminal /

Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily /

Ribosomal protein L28 /

Ribosomal protein L35, conserved site /

Ribosomal protein L35 signature. /

Ribosomal protein L33, conserved site /

Ribosomal protein L33 signature. /

Ribosomal protein L35, non-mitochondrial /

Ribosomal protein L5, bacterial-type /

Ribosomal protein L6, bacterial-type /

Ribosomal protein L18, bacterial-type /

Ribosomal protein L19, conserved site /

Ribosomal protein L19 signature. /

Ribosomal protein L36 /

Ribosomal protein L36 superfamily /

Ribosomal protein L36 /

Ribosomal protein L20 signature. /

Ribosomal protein S3, bacterial-type /

Ribosomal protein L27, conserved site /

Ribosomal protein S6, conserved site /

Ribosomal protein L27 signature. /

Ribosomal protein S6 signature. /

Ribosomal protein S7, bacterial/organellar-type /

Ribosomal protein S11, bacterial-type /

Ribosomal protein S13, bacterial-type /

Ribosomal protein S20 /

Ribosomal protein S20 superfamily /

Ribosomal protein S20 /

Ribosomal protein S9, bacterial/plastid /

Ribosomal protein L14P, bacterial-type /

Ribosomal protein S4, bacterial-type /

Ribosomal protein L34, conserved site /

Ribosomal protein L34 signature. /

30S ribosomal protein S17 /

Ribosomal protein S5, bacterial-type /

Ribosomal protein S6, plastid/chloroplast /

Ribosomal protein L35 /

Ribosomal protein L35 superfamily /

Ribosomal protein L2, bacterial/organellar-type /

Ribosomal protein L35 / Ribosomal L28 family /

Ribosomal protein L33 /

Ribosomal protein L28/L24 /

Ribosomal protein L33 superfamily /

Ribosomal protein L30, bacterial-type /

Ribosomal protein L16 /

Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast /

Ribosomal protein S18, conserved site /

Ribosomal protein S18 signature. / L28p-like /

Ribosomal protein L20 /

Ribosomal protein L20, C-terminal /

Ribosomal protein L21 /

Ribosomal protein L27 / Ribosomal L27 protein /

Ribosomal protein L19 /

Ribosomal protein L19 superfamily /

Ribosomal protein L19 /

Ribosomal proteins 50S L24/mitochondrial 39S L24 /

Ribosomal protein S15, bacterial-type /

Ribosomal protein L17 /

Ribosomal protein L17 superfamily /

Ribosomal protein L17 /

Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein /

Ribosomal protein S6 /

Ribosomal protein S6 superfamily /

Ribosomal protein L24 /

Ribosomal protein S12, bacterial-type /

Ribosomal protein L34 /

Ribosomal protein L13, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 /

Ribosomal protein L3, bacterial/organelle-type /

Ribosomal protein L15, bacterial-type /

Ribosomal protein S18 /

50S ribosomal protein uL4
Similarity search - Domain/homology

Biological species

Staphylococcus aureus (bacteria)

Method

single particle reconstruction /

cryo EM / Resolution: 2.86 Å

Authors

Cimicata G / Bashan A / Yonath A

Funding support

1 items

Citation

Journal: To Be Published
Title: Staphylococcus aureus 50S after 30 minutes incubation a 37C
Authors: Cimicata G / Bashan A / Yonath A

History

Deposition	Oct 27, 2020	-
Header (metadata) release	Nov 17, 2021	-
Map release	Nov 17, 2021	-
Update	Mar 13, 2024	-
Current status	Mar 13, 2024	Processing site: PDBe / Status: Released

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Structure visualization

Movie	Surface view with section colored by density value Surface level: 0.0045 Imaged by UCSF Chimera Download Surface view colored by height Surface level: 0.0045 Imaged by UCSF Chimera Download Surface view with fitted model Atomic models: PDB-7asp Surface level: 0.0045 Imaged by UCSF Chimera Download Movie viewer
Structure viewer	EM map: SurfViewMolmilJmol/JSmol
Supplemental images	emd_11903.png

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Downloads & links

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EMDB archive

Map data	emd_11903.map.gz	336.9 MB		EMDB map data format
Header (meta data)	emd-11903-v30.xml emd-11903.xml	61.8 KB 61.8 KB	Display Display	EMDB header
Images	emd_11903.png	117.4 KB
Filedesc metadata	emd-11903.cif.gz	11.8 KB
Others	emd_11903_additional_1.map.gz	395.4 MB
Archive directory	http://ftp.pdbj.org/pub/emdb/structures/EMD-11903 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-11903	HTTPS FTP

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Related structure data

Related structure data	7aspMC 11900C 11901C 11902C 7asmC 7asnC 7asoC M: atomic model generated by this map C: citing same article (ref.)
Similar structure data	21494 30598 1615 3618 8004 5267 30611 0656 4478 5234 Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

EMDB pages	EMDB (EBI/PDBe) / EMDataResource
Related items in Molecule of the Month	#121 - Jan 2010 70S Ribosomes similarity (8) #253 - Jan 2021 Expressome similarity (7) #157 - Jan 2013 Transfer-Messenger RNA similarity (12) #262 - Oct 2021 Fifty Years of Open Access to PDB Structures similarity (6) #146 - Feb 2012 Aminoglycoside Antibiotics similarity (1) #226 - Oct 2018 Aminoglycoside Antibiotics and Resistance similarity (1) #10 - Oct 2000 Ribosome similarity (1)

EMDB pages

EMDB (EBI/PDBe) /

EMDataResource

Related items in Molecule of the Month

#121 - Jan 2010
70S Ribosomes
similarity (8)
#253 - Jan 2021
Expressome
similarity (7)
#157 - Jan 2013
Transfer-Messenger RNA
similarity (12)
#262 - Oct 2021
Fifty Years of Open Access to PDB Structures
similarity (6)
#146 - Feb 2012
Aminoglycoside Antibiotics
similarity (1)
#226 - Oct 2018
Aminoglycoside Antibiotics and Resistance
similarity (1)
#10 - Oct 2000
Ribosome
similarity (1)

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Map

File

Download / File: emd_11903.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)

Projections & slices

Image control

Size
Brightness
Contrast
Others	InvertY flip

Axes	Z (Sec.)	Y (Row.)	X (Col.)
	0.85 Å/pix. x 480 pix. = 408. Å	0.85 Å/pix. x 480 pix. = 408. Å	0.85 Å/pix. x 480 pix. = 408. Å
Surface
Projections
Slices (1/3)
Slices (1/2)
Slices (2/3)

Images are generated by Spider.

Voxel size

X=Y=Z: 0.85 Å

Density

Histogram

Histogram (log scale)

Contour Level	By AUTHOR: 0.0045 / Movie #1: 0.0045
Minimum - Maximum	-0.028459543 - 0.075757116
Average (Standard dev.)	0.00015371009 (±0.0030464304)

Symmetry

Space group: 1

Details

EMDB XML:

Map geometry

Axis order	X	Y	Z
Origin	0	0	0
Dimensions	480	480	480
Spacing	480	480	480

Cell

A=B=C: 408.0 Å
α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	0.85	0.85	0.85
M x/y/z	480	480	480
origin x/y/z	0.000	0.000	0.000
length x/y/z	408.000	408.000	408.000
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	480	480	480
D min/max/mean	-0.028	0.076	0.000

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Supplemental data

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Additional map: #1

File

emd_11903_additional_1.map

Projections & Slices

Axes	Z	Y	X
Projections
Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : 70S

Entire	Name: 70S
Components	Complex: 70S RNA: 23S rRNA23S ribosomal RNA RNA: 16S rRNA RNA: 5S rRNA5S ribosomal RNA Protein or peptide: 50S ribosomal protein L33,50S ribosomal protein L30Ribosome Protein or peptide: 50S ribosomal protein L34 Protein or peptide: 50S ribosomal protein L35 Protein or peptide: 50S ribosomal protein L36 Protein or peptide: 30S ribosomal protein S10 Protein or peptide: 30S ribosomal protein S11 Protein or peptide: 30S ribosomal protein S12 Protein or peptide: 50S ribosomal protein L2 Protein or peptide: 30S ribosomal protein S13 Protein or peptide: 50S ribosomal protein L3 Protein or peptide: 50S ribosomal protein L4 Protein or peptide: 30S ribosomal protein S14 type ZRibosome Protein or peptide: 30S ribosomal protein S15 Protein or peptide: 30S ribosomal protein S16 Protein or peptide: 50S ribosomal protein L6 Protein or peptide: 50S ribosomal protein L13 Protein or peptide: 30S ribosomal protein S17 Protein or peptide: 50S ribosomal protein L14 Protein or peptide: 30S ribosomal protein S18 Protein or peptide: 30S ribosomal protein S20 Protein or peptide: 50S ribosomal protein L15 Protein or peptide: 30S ribosomal protein S3 Protein or peptide: 50S ribosomal protein L16 Protein or peptide: 50S ribosomal protein L17 Protein or peptide: 30S ribosomal protein S4 Protein or peptide: 30S ribosomal protein S5 Protein or peptide: 50S ribosomal protein L18 Protein or peptide: 30S ribosomal protein S6 Protein or peptide: 50S ribosomal protein L19 Protein or peptide: 30S ribosomal protein S8 Protein or peptide: 50S ribosomal protein L20 Protein or peptide: 30S ribosomal protein S7 Protein or peptide: 50S ribosomal protein L21 Protein or peptide: 30S ribosomal protein S9 Protein or peptide: 50S ribosomal protein L22 Protein or peptide: 50S ribosomal protein L23 Protein or peptide: 50S ribosomal protein L24 Protein or peptide: 50S ribosomal protein L25 Protein or peptide: 50S ribosomal protein L27 Protein or peptide: 50S ribosomal protein L28 Protein or peptide: 50S ribosomal protein L29 Protein or peptide: 50S ribosomalprotein L32p Protein or peptide: 50S ribosomal protein L5

Entire

Name: 70S

Components

Complex: 70S
- RNA: 23S rRNA23S ribosomal RNA
- RNA: 16S rRNA
- RNA: 5S rRNA5S ribosomal RNA
- Protein or peptide: 50S ribosomal protein L33,50S ribosomal protein L30Ribosome
- Protein or peptide: 50S ribosomal protein L34
- Protein or peptide: 50S ribosomal protein L35
- Protein or peptide: 50S ribosomal protein L36
- Protein or peptide: 30S ribosomal protein S10
- Protein or peptide: 30S ribosomal protein S11
- Protein or peptide: 30S ribosomal protein S12
- Protein or peptide: 50S ribosomal protein L2
- Protein or peptide: 30S ribosomal protein S13
- Protein or peptide: 50S ribosomal protein L3
- Protein or peptide: 50S ribosomal protein L4
- Protein or peptide: 30S ribosomal protein S14 type ZRibosome
- Protein or peptide: 30S ribosomal protein S15
- Protein or peptide: 30S ribosomal protein S16
- Protein or peptide: 50S ribosomal protein L6
- Protein or peptide: 50S ribosomal protein L13
- Protein or peptide: 30S ribosomal protein S17
- Protein or peptide: 50S ribosomal protein L14
- Protein or peptide: 30S ribosomal protein S18
- Protein or peptide: 30S ribosomal protein S20
- Protein or peptide: 50S ribosomal protein L15
- Protein or peptide: 30S ribosomal protein S3
- Protein or peptide: 50S ribosomal protein L16
- Protein or peptide: 50S ribosomal protein L17
- Protein or peptide: 30S ribosomal protein S4
- Protein or peptide: 30S ribosomal protein S5
- Protein or peptide: 50S ribosomal protein L18
- Protein or peptide: 30S ribosomal protein S6
- Protein or peptide: 50S ribosomal protein L19
- Protein or peptide: 30S ribosomal protein S8
- Protein or peptide: 50S ribosomal protein L20
- Protein or peptide: 30S ribosomal protein S7
- Protein or peptide: 50S ribosomal protein L21
- Protein or peptide: 30S ribosomal protein S9
- Protein or peptide: 50S ribosomal protein L22
- Protein or peptide: 50S ribosomal protein L23
- Protein or peptide: 50S ribosomal protein L24
- Protein or peptide: 50S ribosomal protein L25
- Protein or peptide: 50S ribosomal protein L27
- Protein or peptide: 50S ribosomal protein L28
- Protein or peptide: 50S ribosomal protein L29
- Protein or peptide: 50S ribosomalprotein L32p
- Protein or peptide: 50S ribosomal protein L5

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Supramolecule #1: 70S

Supramolecule	Name: 70S / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)	Organism: Staphylococcus aureus (bacteria)

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Macromolecule #1: 23S rRNA

Macromolecule	Name: 23S rRNA / type: rna / ID: 1 Details: ...Details: GAUUAAGUUAUUAAGGGCGCACGGUGGAUGCCUUGGCACUAGAAGCCGAUGAAGGACGUUACUAACGACGAUAUGCUUUGGGGAGCUGUAAGCUUUGAUCCAGAGAUUUCCGAAUGGGGAAACCCAGCAUGAGUUAUGUCAUGUUAUCGAUAUGUGAAUACAUAGCAUAUCAGAAGGCACACCCGGAGAACUGAAACAUCUUAGUACCCGGAGGAAGAGAAAGAAAAUUCGAUUCCCUUAGUAGCGGCGAGCGAAACGGGAAGAGCCCAAACCAACAAGCUUGCUUGUUGGGGUUGUAGGACACUCUGUACGGAGUUACAAAGGACGACAUUAGACGAAUCAUCUGGAAAGAUGAAUCAAAGAAGGUAAUAAUCCUGUAGUCGAAAAUGUUGUCUCUCUUGAGUGGAUCCUGAGUACGACGGAGCACGUGAAAUUCCGUCGGAAUCUGGGAGGACCAUCUCCUAAGGCUAAAUACUCUCUAGUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGUGAAAAGCACCCCGGAAGGGGAGUGAAAUAGAACCUGAAACCGUGUGCUUACAAGUAGUCAGAGCCCGUUAAUGGGUGAUGGCGUGCCUUUUGUAGAAUGAACCGGCGAGUUACGAUUUGAUGCAAGGUUAAGCAGUAAAUGUGGAGCCGUAGCGAAAGCGAGUCUGAAUAGGGCGUUUAGUAUUUGGUCGUAGACCCGAAACCAGGUGAUCUACCCUUGGUCAGGUUGAAGUUCAGGUAACACUGAAUGGAGGACCGAACCGACUUACGUUGAAAAGUGAGCGGAUGAACUGAGGGUAGCGGAGAAAUUCCAAUCGAACCUGGAGAUAGCUGGUUCUCUCCGAAAUAGCUUUAGGGCUAGCCUCAAGUGAUGAUUAUUGGAGGUAGAGCACUGUUUGGACGAGGGGCCCCUCUCGGGUUACCGAAUUCAGACAAACUCCGAAUGCCAAUUAAUUUAACUUGGGAGUCAGAACAUGGGUGAUAAGGUCCGUGUUCGAAAGGGAAACAGCCCAGACCACCAGCUAAGGUCCCAAAAUAUAUGUUAAGUGGAAAAGGAUGUGGCGUUGCCCAGACAACUAGGAUGUUGGCUUAGAAGCAGCCAUCAUUUAAAGAGUGCGUAAUAGCUCACUAGUCGAGUGACACUGCGCCGAAAAUGUACCGGGGCUAAACAUAUUACCGAAGCUGUGGAUUGUCCUUUGGACAAUGGUAGGAGAGCGUUCUAAGGGCGUUGAAGCAUGAUCGUAAGGACAUGUGGAGCGCUUAGAAGUGAGAAUGCCGGUGUGAGUAGCGAAAGACGGGUGAGAAUCCCGUCCACCGAUUGACUAAGGUUUCCAGAGGAAGGCUCGUCCGCUCUGGGUUAGUCGGGUCCUAAGCUGAGGCCGACAGGCGUAGGCGAUGGAUAACAGGUUGAUAUUCCUGUACCACCUAUAAUCGUUUUAAUCGAUGGGGGGACGCAGUAGGAUAGGCGAAGCGUGCGAUUGGAUUGCACGUCUAAGCAGUAAGGCUGAGUAUUAGGCAAAUCCGGUACUCGUUAAGGCUGAGCUGUGAUGGGGAGAAGACAUUGAGUCUUCGAGUCGUUGAUUUCACACUGCCGAGAAAAGCCUCUAGAUAGAAAAUAGGUGCCCGUACCGCAAACCGACACAGGUAGUCAAGAUGAGAAUUCUAAGGUGAGCGAGCGAACUCUCGUUAAGGAACUCGGCAAAAUGACCCCGGUAACUUCGGGAGAAGGGGUGCUCUUUAGGGUUAACGCCCAGAAGAGCCGCAGUGAAUAGGCCCAAGCGACUGUUUAUCAAAAACACAGGUCUCUGCUAAACCGUAAGGUGAUGUAUAGGGGCUGACGCCUGCCCGGUGCUGGAAGGUUAAGAGGAGUGGUUAGCUUCUGCGAAGCUACGAAUCGAAGCCCCAGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGAAAGGCGUAACGAUUUGGGCACUGUCUCAACGAGAGACUCGGUGAAAUCAUAGUACCUGUGAAGAUGCAGGUUACCCGCGACAGGACGGAAAGACCCCGUGGAGCUUUACUGUAGCCUGAUAUUGAAAUUCGGCACAGCUUGUACAGGAUAGGUAGGAGCCUUUGAAACGUGAGCGCUAGCUUACGUGGAGGCGCUGGUGGGAUACUACCCUAGCUGUGUUGGCUUUCUAACCCGCACCACUUAUCGUGGUGGGAGACAGUGUCAAGCGGGCAGUUUGACUGGGGCGGUCGCCUCCUAAAAGGUAACGGAGGCGCUCAAAGGUUCCCUCAGAAUGGUUGGAAAUCAUUCAUAGAGUGUAAAGGCAUAAGGGAGCUUGACUGCGAGACCUACAAGUCGAGCAGGGUCGAAAGACGGACUUAGUGAUCCGGUGGUUCCGCAUGGAAGGGCCAUCGCUCAACGGAUAAAAGCUACCCCGGGGAUAACAGGCUUAUCUCCCCCAAGAGUUCACAUCGACGGGGAGGUUUGGCACCUCGAUGUCGGCUCAUCGCAUCCUGGGGCUGUAGUCGGUCCCAAGGGUUGGGCUGUUCGCCCAUUAAAGCGGUACGCGAGCUGGGUUCAGAACGUCGUGAGACAGUUCGGUCCCUAUCCGUCGUGGGCGUAGGAAAUUUGAGAGGAGCUGUCCUUAGUACGAGAGGACCGGGAUGGACAUACCUCUGGUGUACCAGUUGUCGUGCCAACGGCAUAGCUGGGUAGCUAUGUGUGGACGGGAUAAGUGCUGAAAGCAUCUAAGCAUGAAGCCCCCCUCAAGAUGAGAUUUCCCAACUUCGGUUAUAAGAUCCCUCAAAGAUGAUGAGGUUAAUAGGUUCGAGGUGGAAGCAUGGUGACAUGUGGAGCUGACGAAUACUAAUCGAUCGAAGACUUAAUCAA Number of copies: 1
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 946.687688 KDa
Sequence	String: GAUUAAGUUA UUAAGGGCGC ACGGUGGAUG CCUUGGCACU AGAAGCCAAU GAAGGACGUU ACUAACGACG AUAUGCUUUG GGGAGCUGU AAGUAAGCUU UGAUCCAGAG AUUUCCGAAU GGGGAAACCC AGCAUGAGUU AUGUCAUGUU AUCGAUAUGU G AAUACAUA ...String: GAUUAAGUUA UUAAGGGCGC ACGGUGGAUG CCUUGGCACU AGAAGCCAAU GAAGGACGUU ACUAACGACG AUAUGCUUUG GGGAGCUGU AAGUAAGCUU UGAUCCAGAG AUUUCCGAAU GGGGAAACCC AGCAUGAGUU AUGUCAUGUU AUCGAUAUGU G AAUACAUA GCAUAUCAGA AGGCACACCC GGAGAACUGA AACAUCUUAG UACCCGGAGG AAGAGAAAGA AAAUUCGAUU CC CUUAGUA GCGGCGAGCG AAACGGGAAG AGCCCAAACC AACAAGCUUG CUUGUUGGGG UUGUAGGACA CUCUAUACGG AGU UACAAA GGACGACAUU AGACGAAUCA UCUGGAAAGA UGAAUCAAAG AAGGUAAUAA UCCUGUAGUC GAAAAUGUUG UCUC UCUUG AGUGGAUCCU GAGUACGACG GAGCACGUGA AAUUCCGUCG GAAUCUGGGA GGACCAUCUC CUAAGGCUAA AUACU CUCU AGUGACCGAU AGUGAACCAG UACCGUGAGG GAAAGGUGAA AAGCACCCCG GAAGGGGAGU GAAAUAGAAC CUGAAA CCG UGUGCUUACA AGUAGUCAGA GCCCGUUAAU GGGUGAUGGC GUGCCUUUUG UAGAAUGAAC CGGCGAGUUA CGAUUUG AU GCAAGGUUAA GCAGUAAAUG UGGAGCCGUA GCGAAAGCGA GUCUGAAUAG GGCGUUUAGU AUUUGGUCGU AGACCCGA A ACCAGGUGAU CUACCCUUGG UCAGGUUGAA GUUCAGGUAA CACUGAAUGG AGGACCGAAC CGACUUACGU UGAAAAGUG AGCGGAUGAA CUGAGGGUAG CGGAGAAAUU CCAAUCGAAC CUGGAGAUAG CUGGUUCUCU CCGAAAUAGC UUUAGGGCUA GCCUCAAGU GAUGAUUAUU GGAGGUAGAG CACUGUUUGG ACGAGGGGCC CCUCUCGGGU UACCGAAUUC AGACAAACUC C GAAUGCCA AUUAAUUUAA CUUGGGAGUC AGAACAUGGG UGAUAAGGUC CGUGUUCGAA AGGGAAACAG CCCAGACCAC CA GCUAAGG UCCCAAAAUA UAUGUUAAGU GGAAAAGGAU GUGGCGUUGC CCAGACAACU AGGAUGUUGG CUUAGAAGCA GCC AUCAUU UAAAGAGUGC GUAAUAGCUC ACUAGUCGAG UGACACUGCG CCGAAAAUGU ACCGGGGCUA AACAUAUUAC CGAA GCUGU GGAUUGUCCU UUGGACAAUG GUAGGAGAGC GUUCUAAGGG CGUUGAAGCA UGAUCGUAAG GACAUGUGGA GCGCU UAGA AGUGAGAAUG CCGGUGUGAG UAGCGAAAGA CGGGUGAGAA UCCCGUCCAC CGAUUGACUA AGGUUUCCAG AGGAAG GCU CGUCCGCUCU GGGUUAGUCG GGUCCUAAGC UGAGGCCGAC AGGCGUAGGC GAUGGAUAAC AGGUUGAUAU UCCUGUA CC ACCUAUAAUC GUUUUAAUCG AUGGGGGGAC GCAGUAGGAU AGGCGAAGCG UGCGAUUGGA UUGCACGUCU AAGCAGUA A GGCUGAGUAU UAGGCAAAUC CGGUACUCGU UAAGGCUGAG CUGUGAUGGG GAGAAGACAU UGAGUCUUCG AGUCGUUGA UUUCACACUG CCGAGAAAAG CCUCUAGAUA GAAAAUAGGU GCCCGUACCG CAAACCGACA CAGGUAGUCA AGAUGAGAAU UCUAAGGUG AGCGAGCGAA CUCUCGUUAA GGAACUCGGC AAAAUGACCC CGUAACUUCG GGAGAAGGGG UGCUCUUUAG G GUUAACGC CCAGAAGAGC CGCAGUGAAU AGGCCCAAGC GACUGUUUAU CAAAAACACA GGUCUCUGCU AAACCGUAAG GU GAUGUAU AGGGGCUGAC GCCUGCCCGG UGCUGGAAGG UUAAGAGGAG UGGUUAGCUU CUGCGAAGCU ACGAAUCGAA GCC CCAGUA AACGGCGGCC GUAACUAUAA CGGUCCUAAG GUAGCGAAAU UCCUUGUCGG GUAAGUUCCG ACCCGCACGA AAGG CGUAA CGAUUUGGGC ACUGUCUCAA CGAGAGACUC GGUGAAAUCA UAGUACCUGU GAAGAUGCAG GUUACCCGCG ACAGG ACGG AAAGACCCCG UGGAGCUUUA CUGUAGCCUG AUAUUGAAAU UCGGCACAGC UUGUACAGGA UAGGUAGGAG CCUUUG AAA CGUGAGCGCU AGCUUACGUG GAGGCGCUGG UGGGAUACUA CCCUAGCUGU GUUGGCUUUC UAACCCGCAC CACUUAU CG UGGUGGGAGA CAGUGUCAAG CGGGCAGUUU GACUGGGGCG GUCGCCUCCU AAAAGGUAAC GGAGGCGCUC AAAGGUUC C CUCAGAAUGG UUGGAAAUCA UUCAUAGAGU GUAAAGGCAU AAGGGAGCUU GACUGCGAGA CCUACAAGUC GAGCAGGGU CGAAAGACGG ACUUAGUGAU CCGGUGGUUC CGCAUGGAAG GGCCAUCGCU CAACGGAUAA AAGCUACCCC GGGGAUAACA GGCUUAUCU CCCCCAAGAG UUCACAUCGA CGGGGAGGUU UGGCACCUCG AUGUCGGCUC AUCGCAUCCU GGGGCUGUAG U CGGUCCCA AGGGUUGGGC UGUUCGCCCA UUAAAGCGGU ACGCGAGCUG GGUUCAGAAC GUCGUGAGAC AGUUCGGUCC CU AUCCGUC GUGGGCGUAG GAAAUUUGAG AGGAGCUGUC CUUAGUACGA GAGGACCGGG AUGGACAUAC CUCUGGUGUA CCA GUUGUC GUGCCAACGG CAUAGCUGGG UAGCUAUGUG UGGACGGGAU AAGUGCUGAA AGCAUCUAAG CAUGAAGCCC CCCU CAAGA UGAGAUUUCC CAACUUCGGU UAUAAGAUCC CUCAAAGAUG AUGAGGUUAA UAGGUUCGAG GUGGAAGCAU GGUGA CAUG UGGAGCUGAC GAAUACUAAU CGAUCGAAGA CUUAAUCAA

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Macromolecule #2: 16S rRNA

Macromolecule	Name: 16S rRNA / type: rna / ID: 2 Details: ...Details: UUUAUGGAGAGUUUGAUCCUGGCUCAGGAUGAACGCUGGCGGCGUGCCUAAUACAUGCAAGUCGAGCGAACGGACGAGAAGCUUGCUUCUCUGAUGUUAGCGGCGGACGGGUGAGUAACACGUGGAUAACCUACCUAUAAGACUGGGAUAACUUCGGGAAACCGGAGCUAAUACCGGAUAAUAUUUUGAACCGCAUGGUUCAAAAGUGAAAGACGGUCUUGCUGUCACUUAUAGAUGGAUCCGCGCUGCAUUAGCUAGUUGGUAAGGUAACGGCUUACCAAGGCAACGAUACGUAGCCGACCUGAGAGGGUGAUCGGCCACACUGGAACUGAGACACGGUCCAGACUCCUACGGGAGGCAGCAGUAGGGAAUCUUCCGCAAUGGGCGAAAGCCUGACGGAGCAACGCCGCGUGAGUGAUGAAGGUCUUCGGAUCGUAAAACUCUGUUAUUAGGGAAGAACAUAUGUGUAAGUAACUGUGCACAUCUUGACGGUACCUAAUCAGAAAGCCACGGCUAACUACGUGCCAGCAGCCGCGGUAAUACGUAGGUGGCAAGCGUUAUCCGGAAUUAUUGGGCGUAAAGCGCGCGUAGGCGGUUUUUUAAGUCUGAUGUGAAAGCCCACGGCUCAACCGUGGAGGGUCAUUGGAAACUGGAAAACUUGAGUGCAGAAGAGGAAAGUGGAAUUCCAUGUGUAGCGGUGAAAUGCGCAGAGAUAUGGAGGAACACCAGUGGCGAAGGCGACUUUCUGGUCUGUAACUGACGCUGAUGUGCGAAAGCGUGGGGAUCAAACAGGAUUAGAUACCCUGGUAGUCCACGCCGUAAACGAUGAGUGCUAAGUGUUAGGGGGUUUCCGCCCCUUAGUGCUGCAGCUAACGCAUUAAGCACUCCGCCUGGGGAGUACGACCGCAAGGUUGAAACUCAAAGGAAUUGACGGGGACCCGCACAAGCGGUGGAGCAUGUGGUUUAAUUCGAAGCAACGCGAAGAACCUUACCAAAUCUUGACAUCCUUUGACAACUCUAGAGAUAGAGCCUUCCCCUUCGGGGGACAAAGUGACAGGUGGUGCAUGGUUGUCGUCAGCUCGUGUCGUGAGAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUAAGCUUAGUUGCCAUCAUUAAGUUGGGCACUCUAAGUUGACUGCCGGUGACAAACCGGAGGAAGGUGGGGAUGACGUCAAAUCAUCAUGCCCCUUAUGAUUUGGGCUACACACGUGCUACAAUGGACAAUACAAAGGGCAGCGAAACCGCGAGGUCAAGCAAAUCCCAUAAAGUUGUUCUCAGUUCGGAUUGUAGUCUGCAACUCGACUACAUGAAGCUGGAAUCGCUAGUAAUCGUAGAUCAGCAUGCUACGGUGAAUACGUUCCCGGGUAUUGUACACACCGCCCGUCACACCACGAGAGUUUGUAACACCCGAAGCCGGUGGAGUAACCUUUUAGGAGCUAGCCGUCGAAGGUGGGACAAAUGAUUGGGGUGAAGUCGUAACAAGGUAGCCGUAUCGGAAGGUGCGGCUGGAUCACCUCCUUU Number of copies: 1
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 502.019281 KDa
Sequence	String: UUUAUGGAGA GUUUGAUCCU GGCUCAGGAU GAACGCUGGC GGCGUGCCUA AUACAUGCAA GUCGAGCGAA CGGACGAGAA GCUUGCUUC UCUGAUGUUA GCGGCGGACG GGUGAGUAAC ACGUGGAUAA CCUACCUAUA AGACUGGGAU AACUUCGGGA A ACCGUAGC ...String: UUUAUGGAGA GUUUGAUCCU GGCUCAGGAU GAACGCUGGC GGCGUGCCUA AUACAUGCAA GUCGAGCGAA CGGACGAGAA GCUUGCUUC UCUGAUGUUA GCGGCGGACG GGUGAGUAAC ACGUGGAUAA CCUACCUAUA AGACUGGGAU AACUUCGGGA A ACCGUAGC UAAUACCGGA UAAUAUUUUG AACCGCAUGG UUCAAAAGUG AAAGACGGUC UUGCUGUCAC UUAUAGAUGG AU CCGCGCU GCAUUAGCUA GUUGGUAAGG UAACGGCUUA CCAAGGCAAC GAUGCAUAGC CGACCUGAGA GGGUGAUCGG CCA CACUGG AACUGAGACA CGGUCCAGAC UCCUACGGGA GGCAGCAGUA GGGAAUCUUC CGCAAUGGGC GAAAGCCUGA CGGA GCAAC GCCGCGUGAG UGAUGAAGGU CUUCGGAUCG UAAAACUCUG UUAUUAGGGA AGAACAUAUG UGUAAGUAAC UGUGC ACAU CUUGACGGUA CCUAAUCAGA AAGCCACGGC UAACUACGUG CCAGCAGCCG CGGUAAUACG UAGGUGGCAA GCGUUA UCC GGAAUUAUUG GGCGUAAAGC GCGCGUAGGC GGUUUUUUAA GUCUGAUGUG AAAGCCCACG GCUCAACCGU GGAGGGU CA UUGGAAACUG GAAAACUUGA GUGCAGAAGA GGAAAGUGGA AUUCCAUGUG UAGCGGUGAA AUGCGCAGAG AUAUGGAG G AACACCAGUG GCGAAGGCGA CUUUCUGGUC UGUAACUGAC GCUGAUGUGC GAAAGCGUGG GGAUCAAACA GGAUUAGAU ACCCUGGUAG UCCACGCCGU AAACGAUGAG UGCUAAGUGU UAGGGGGUUU CCGCCCCUUA GUGCUGCAGC UAACGCAUUA AGCACUCCG CCUGGGGAGU ACGACCGCAA GGUUGAAACU CAAAGGAAUU GACGGGGACC CGCACAAGCG GUGGAGCAUG U GGUUUAAU UCGAAGCAAC GCGAAGAACC UUACCAAAUC UUGACAUCCU UUGACAACUC UAGAGAUAGA GCCUUCCCCU UC GGGGGAC AAAGUGACAG GUGGUGCAUG GUUGUCGUCA GCUCGUGUCG UGAGAUGUUG GGUUAAGUCC CGCAACGAGC GCA ACCCUU AAGCUUAGUU GCCAUCAUUA AGUUGGGCAC UCUAAGUUGA CUGCCGGUGA CAAACCGGAG GAAGGUGGGG AUGA CGUCA AAUCAUCAUG CCCCUUAUGA UUUGGGCUAC ACACGUGCUA CAAUGGACAA UACAAAGGGC AGCGAAACCG CGAGG UCAA GCAAAUCCCA UAAAGUUGUU CUCAGUUCGG AUUGUAGUCU GCAACUCGAC UACAUGAAGC UGGAAUCGCU AGUAAU CGU AGAUCAGCAU GCUACGGUGA AUACGUUCCC GGGUCUUGUA CACACCGCCC GUCACACCAC GAGAGUUUGU AACACCC GA AGCCGGUGGA GUAACCUUUU AGGAGCUAGC CGUCGAAGGU GGGACAAAUG AUUGGGGUGA AGUCGUAACA AGGUAGCC G UAUCGGAAGG UGCGGCUGGA UCACCUCCUU U

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Macromolecule #3: 5S rRNA

Macromolecule	Name: 5S rRNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 36.692801 KDa
Sequence	String: UCUGGUGACU AUAGCAAGGA GGUCACACCU GUUCCCAUGC CGAACACAGA AGUUAAGGUC UUUAGCGACG AUGGUAGCCA ACUUACGUU CCGCUAGAGU AGAACGUUGC CAGGC

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Macromolecule #4: 50S ribosomal protein L33,50S ribosomal protein L30

Macromolecule	Name: 50S ribosomal protein L33,50S ribosomal protein L30 / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 12.101037 KDa
Sequence	String: RVNVTLACTE CGDRNYITTK NKRNNPERIE MKKYCPRLNK YTLHRETAKL QITLTRSVIG RPETQRKTVE ALGLKKTNSS VVVEDNPAI RGQINKVKHL VTVEEK UniProtKB: Large ribosomal subunit protein bL33, Large ribosomal subunit protein uL30

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Macromolecule #5: 50S ribosomal protein L34

Macromolecule	Name: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 5.252369 KDa
Sequence	String: VKRTYQPNKR KHSKVHGFRK RMSTKNGRKV LARRRRKGRK VLS UniProtKB: Large ribosomal subunit protein bL34

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Macromolecule #6: 50S ribosomal protein L35

Macromolecule	Name: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 7.461992 KDa
Sequence	String: PKMKTHRGAA KRVKRTASGQ LKRSRAFTSH LFANKSTKQK RQLRKARLVS KSDMKRVKQL LAYK UniProtKB: Large ribosomal subunit protein bL35

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Macromolecule #7: 50S ribosomal protein L36

Macromolecule	Name: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 4.318422 KDa
Sequence	String: MKVRPSVKPI CEKCKVIKRK GKVMVICENP KHKQRQG UniProtKB: Large ribosomal subunit protein bL36

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Macromolecule #8: 30S ribosomal protein S10

Macromolecule	Name: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 9.24361 KDa
Sequence	String: IRIRLKAYDH RVIDQSAEKI VETAKRSGAD VSGPIPLPTE KSVYTIIRAV HKYKDSREQF EQRTHKRLID IVNPTPKTVD UniProtKB: UNIPROTKB: A0A6H0HKY4

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Macromolecule #9: 30S ribosomal protein S11

Macromolecule	Name: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 12.047609 KDa
Sequence	String: NIENGVAHIR STFNNTIVTI TDEFGNALSW SSAGALGFKG SKKSTPFAAQ MASETASKSA MEHGLKTVEV TVKGPGPGRE SAIRALQSA GLEVTAIRDV TPVPHNGCRP PKRRR UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #10: 30S ribosomal protein S12

Macromolecule	Name: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 15.189673 KDa
Sequence	String: PTINQLVRKP RQSKIKKSDS PALNKGFNSK KKKFTDLNSP QKRGVCTRVG TMTPKKPNSA LRKYARVRLS NNIEINAYIP GIGHNLQEH SVVLVRGGRV KDLPGVRYHI VRGALDTSGV DGRRQGRSLY GTKKPKN UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #11: 50S ribosomal protein L2

Macromolecule	Name: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 29.827607 KDa
Sequence	String: AIKKYKPITN GRRNMTSLDF AEITKTTPEK SLLKPLPKKA GRNNQGKLTV RHHGGGHKRQ YRVIDFKRNK DGINAKVDSI QYDPNRSAN IALVVYADGE KRYIIAPKGL EVGQIVESGA EADIKVGNAL PLQNIPVGTV VHNIELKPGK GGQIARSAGA S AQVLGKEG ...String: AIKKYKPITN GRRNMTSLDF AEITKTTPEK SLLKPLPKKA GRNNQGKLTV RHHGGGHKRQ YRVIDFKRNK DGINAKVDSI QYDPNRSAN IALVVYADGE KRYIIAPKGL EVGQIVESGA EADIKVGNAL PLQNIPVGTV VHNIELKPGK GGQIARSAGA S AQVLGKEG KYVLIRLRSG EVRMILSTCR ATIGQVGNLQ HELVNVGKAG RSRWKGIRPT VRGSVMNPND HPHGGGEGRA PI GRPSPMS PWGKPTLGKK TRRGKKSSDK LIVRGRK UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #12: 30S ribosomal protein S13

Macromolecule	Name: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 12.843768 KDa
Sequence	String: ARIAGVDIPR EKRVVISLTY IYGIGTSTAQ KILEEANVSA DTRVKDLTDD ELGRIREVVD GYKVEGDLRR ETNLNIKRLM EISSYRGIR HRRGLPVRGQ KTKNNARTRK GPVK UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #13: 50S ribosomal protein L3

Macromolecule	Name: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 23.199545 KDa
Sequence	String: TKGILGRKIG MTQVFGENGE LIPVTVVEAK ENVVLQKKTV EVDGYNAIQV GFEDKKAYKK DAKSNKYANK PAEGHAKKAD AAPKRFIRE FRNVDVDAYE VGQEVSVDTF VAGDVIDVTG VSKGKGFQGA IKRHGQSRGP MSHGSHFHRA PGSVGMASDA S RVFKGQKM ...String: TKGILGRKIG MTQVFGENGE LIPVTVVEAK ENVVLQKKTV EVDGYNAIQV GFEDKKAYKK DAKSNKYANK PAEGHAKKAD AAPKRFIRE FRNVDVDAYE VGQEVSVDTF VAGDVIDVTG VSKGKGFQGA IKRHGQSRGP MSHGSHFHRA PGSVGMASDA S RVFKGQKM PGRMGGNTVT VQNLEVVQVD TENKVILVKG NVPGPKKGLV EIRTSIK UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #14: 50S ribosomal protein L4

Macromolecule	Name: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 22.364504 KDa
Sequence	String: ANYDVLKLDG TKSGSIELSD AVFGIEPNNS VLFEAINLQR ASLRQGTHAV KNRSAVSGGG RKPWKQKGTG RARQGTIRAP QWRGGGIVF GPTPRSYAYK MPKKMRRLAL RSALSFKAQE NGLTVVDAFN FEAPKTKEFK NVLSTLEQPK KVLVVTENED V NVELSARN ...String: ANYDVLKLDG TKSGSIELSD AVFGIEPNNS VLFEAINLQR ASLRQGTHAV KNRSAVSGGG RKPWKQKGTG RARQGTIRAP QWRGGGIVF GPTPRSYAYK MPKKMRRLAL RSALSFKAQE NGLTVVDAFN FEAPKTKEFK NVLSTLEQPK KVLVVTENED V NVELSARN IPGVQVTTAQ GLNVLDITNA DSLVITEAAA KKVEEVLG UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #15: 30S ribosomal protein S14 type Z

Macromolecule	Name: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 7.186573 KDa
Sequence	String: AKTSMVAKQQ KKQKYAVREY TRCERCGRPH SVYRKFKLCR ICFRELAYKG QIPGVRKASW UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #16: 30S ribosomal protein S15

Macromolecule	Name: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 10.503135 KDa
Sequence	String: AISQERKNEI IKEYRVHETD TGSPEVQIAV LTAEINAVNE HLRTHKKDHH SRRGLLKMVG RRRHLLNYLR SKDIQRYREL IKSLGIRR UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #17: 30S ribosomal protein S16

Macromolecule	Name: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 9.291743 KDa
Sequence	String: AVKIRLTRLG SKRNPFYRIV VADARSPRDG RIIEQIGTYN PTSANAPEIK VDEALALKWL NDGAKPTDTV HNILSKEGIM KKF UniProtKB: UNIPROTKB: A0A6K3WEJ9

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Macromolecule #18: 50S ribosomal protein L6

Macromolecule	Name: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 19.501154 KDa
Sequence	String: SRVGKKIIDI PSDVTVTFDG NHVTVKGPKG ELSRTLNERM TFKQEENTIE VVRPSDSKED RTNHGTTRAL LNNMVQGVSQ GYVKVLELV GVGYRAQMQG KDLILNVGYS HPVEIKAEEN ITFSVEKNTV VKVEGISKEQ VGALASNIRS VRPPEPYKGK G IRYQGEYV RRKEGKT UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #19: 50S ribosomal protein L13

Macromolecule	Name: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 16.359427 KDa
Sequence	String: MRQTFMANES NIERKWYVID AEGQTLGRLS SEVASILRGK NKVTYTPHVD TGDYVIVINA SKIEFTGNKE TDKVYYRHSN HPGGIKSIT AGELRRTNPE RLIENSIKGM LPSTRLGEKQ GKKLFVYGGA EHPHAAQQPE NYELRG UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #20: 30S ribosomal protein S17

Macromolecule	Name: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 9.36686 KDa
Sequence	String: NDRKVYVGKV VSDKMDKTIT VLVETYKTHK LYGKRVKYSK KYKTHDENNS AKLGDIVKIQ ETRPLSATKR FRLVEIVEES UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #21: 50S ribosomal protein L14

Macromolecule	Name: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 13.157342 KDa
Sequence	String: MIQQETRLKV ADNSGAREVL TIKVLGGSGR KTANIGDVIV CTVKNATPGG VVKKGDVVKA VIVRTKSGVR RNDGSYIKFD ENACVIIRD DKGPRGTRIF GPVARELREG NFMKIVSLAP EVL UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #22: 30S ribosomal protein S18

Macromolecule	Name: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 6.574833 KDa
Sequence	String: GITHIDYKDT ELLKRFISER GKILPRRVTG TSAKYQRMLT TAIKRSRHMA LLPYVK UniProtKB: Small ribosomal subunit protein bS18

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Macromolecule #23: 30S ribosomal protein S20

Macromolecule	Name: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 8.480758 KDa
Sequence	String: KSAIKRVKTT EKAEARNISQ KSAMRTAVKN AKTAVSNNAD NKNELVSLAV KLVDKAAQSN LIHSNKADRI KSQLMTAN UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #24: 50S ribosomal protein L15

Macromolecule	Name: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 15.62889 KDa
Sequence	String: MKLHELKPAE GSRKERNRVG RGVATGNGKT SGRGHKGQKA RSGGGVRPGF EGGQLPLFRR LPKRGFTNIN RKEYAIVNLD QLNKFEDGT EVTPALLVES GVVKNEKSGI KILGNGSLDK KLTVKAHKFS ASAAEAIDAK GGAHEVI UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #25: 30S ribosomal protein S3

Macromolecule	Name: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 22.684201 KDa
Sequence	String: GQKINPIGLR VGIIRDWEAK WYAEKDFASL LHEDLKIRKF IDNELKEASV SHVEIERAAN RINIAIHTGK PGMVIGKGGS EIEKLRNKL NALTDKKVHI NVIEIKKVDL DARLVAENIA RQLENRASFR RVQKQAITRA MKLGAKGIKT QVSGRLGGAD I ARAEQYSE ...String: GQKINPIGLR VGIIRDWEAK WYAEKDFASL LHEDLKIRKF IDNELKEASV SHVEIERAAN RINIAIHTGK PGMVIGKGGS EIEKLRNKL NALTDKKVHI NVIEIKKVDL DARLVAENIA RQLENRASFR RVQKQAITRA MKLGAKGIKT QVSGRLGGAD I ARAEQYSE GTVPLHTLRA DIDYAHAEAD TTYGKLGVKV WIYR UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #26: 50S ribosomal protein L16

Macromolecule	Name: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 15.599435 KDa
Sequence	String: MLLPKRVKYR RQHRPKTTGR SKGGNYVTFG EFGLQATTTS WITSRQIESA RIAMTRYMKR GGKVWIKIFP HTPYTKKPLE VRMGAGKGA VEGWIAVVKP GRILFEVAGV SEEVAREALR LASHKLPVKT KFVKREEL UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #27: 50S ribosomal protein L17

Macromolecule	Name: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 13.583523 KDa
Sequence	String: YRKLGRTSDQ RKAMLRDLAT SLIISERIET TEARAKEVRS VVEKLITLGK KGDLASRRNA AKTLRNVEIL NEDETTQTAL QKLFGEIAE RYTERQGGYT RILKQGPRRG DGAESVIIEL V UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #28: 30S ribosomal protein S4

Macromolecule	Name: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 22.849145 KDa
Sequence	String: RFRGSNWKKS RRLGISLSGT GKELEKRPYA PGQHGPNQRK KLSEYGLQLR EKQKLRYLYG MTERQFRNTF DIAGKKFGVH GENFMILLA SRLDAVVYSL GLARTRRQAR QLVNHGHILV DGKRVDIPSY SVKPGQTISV REKSQKLNII VESVEINNFV P EYLNFDAD ...String: RFRGSNWKKS RRLGISLSGT GKELEKRPYA PGQHGPNQRK KLSEYGLQLR EKQKLRYLYG MTERQFRNTF DIAGKKFGVH GENFMILLA SRLDAVVYSL GLARTRRQAR QLVNHGHILV DGKRVDIPSY SVKPGQTISV REKSQKLNII VESVEINNFV P EYLNFDAD SLTGTFVRLP ERSELPAEIN EQLIVEYYSR UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #29: 30S ribosomal protein S5

Macromolecule	Name: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 16.522119 KDa
Sequence	String: EFEERVVTIN RVAKVVKGGR RFRFTALVVV GDKNGRVGFG TGKAQEVPEA IKKAVEAAKK DLVVVPRVEG TTPHTITGRY GSGSVFMKP AAPGTGVIAG GPVRAVLELA GITDILSKSL GSNTPINMVR ATIDGLQNLK NAEDVAKLRG KTVEELY UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #30: 50S ribosomal protein L18

Macromolecule	Name: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 13.124093 KDa
Sequence	String: MISKIDKNKV RLKRHARVRT NLSGTAEKPR LNVYRSNKHI YAQIIDDNKG VTLAQASSKD SDIATTATKV ELATKVGEAI AKKAADKGI KEIVFDRGGY LYHGRVKALA EAARESGLEF UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #31: 30S ribosomal protein S6

Macromolecule	Name: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 11.239766 KDa
Sequence	String: MRTYEVMYIV RPNIEEDAKK ALVERFNGIL ATEGAEVLEA KDWGKRRLAY EINDFKDGFY NIVRVKSDNN KATDEFQRLA KISDDIIRY MVIRED UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #32: 50S ribosomal protein L19

Macromolecule	Name: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 13.104381 KDa
Sequence	String: TNHKLIEAVT KSQLRTDLPS FRPGDTLRVH VRIIEGTRER IQVFEGVVIK RRGGGVSETF TVRKISSGVG VERTFPLHTP KIEKIEVKR RGKVRRAKLY YLRSLRGKAA RIQEI UniProtKB: Large ribosomal subunit protein bL19

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Macromolecule #33: 30S ribosomal protein S8

Macromolecule	Name: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 14.622014 KDa
Sequence	String: MTDPIADMLT RVRNANMVRH EKLELPASNI KKEIAEILKS EGFIKNVEYV EDDKQGVLRL FLKYGQNDER VITGLKRISK PGLRVYAKA SEMPKVLNGL GIALVSTSEG VITDKEARKR NVGGEIIAYV W UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #34: 50S ribosomal protein L20

Macromolecule	Name: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 13.459919 KDa
Sequence	String: PRVKGGTVTR ARRKKTIKLA KGYFGSKHTL YKVAKQQVMK SGQYAFRDRR QRKRDFRKLW ITRINAAARQ HEMSYSRLMN GLKKAGIDI NRKMLSEIAI SDEKAFAQLV TKAKDAL UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #35: 30S ribosomal protein S7

Macromolecule	Name: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 17.695357 KDa
Sequence	String: PRKGSVPKRD VLPDPIHNSK LVTKLINKIM LDGKRGTAQR ILYSAFDLVE QRSGRDALEV FEEAINNIMP VLEVKARRVG GSNYQVPVE VRPERRTTLG LRWLVNYARL RGEKTMEDRL ANEILDAANN TGGAVKKRED THKMAEANKA FAHYRW UniProtKB: Small ribosomal subunit protein uS7

+
Macromolecule #36: 50S ribosomal protein L21

Macromolecule	Name: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 11.354081 KDa
Sequence	String: MFAIIETGGK QIKVEEGQEI FVEKLDVNEG DTFTFDKVLF VGGDSVKVGA PTVEGATVTA TVNKQGRGKK ITVFTYKRRK NSKRKKGHR QPYTKLTIDK INA UniProtKB: Large ribosomal subunit protein bL21

+
Macromolecule #37: 30S ribosomal protein S9

Macromolecule	Name: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 14.312321 KDa
Sequence	String: VEYRGTGRRK NSVARVRLVP GEGNITVNNR DVREYLPFES LILDLNQPFD VTETKGNYDV LVNVHGGGFT GQAQAIRHGI ARALLEADP EYRGSLKRAG LLTRDPRMKE RKKPGLKAAR RSPQFSKR UniProtKB: Small ribosomal subunit protein uS9

+
Macromolecule #38: 50S ribosomal protein L22

Macromolecule	Name: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 12.328358 KDa
Sequence	String: MEAKAVARTI RIAPRKVRLV LDLIRGKNAA EAIAILKLTN KASSPVIEKV LMSALANAEH NYDMNTDELV VKEAYANEGP TLKRFRPRA QGRASAINKR TSHITIVVSD GKE UniProtKB: UNIPROTKB: A0A6I4H7Y4

+
Macromolecule #39: 50S ribosomal protein L23

Macromolecule	Name: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 10.3801 KDa
Sequence	String: EARDILKRPV ITEKSSEAMA EDKYTFDVDT RVNKTQVKMA VEEIFNVKVA SVNIMNYKPK KKRMGRYQGY TNKRRKAIVT LKEGSIDLF UniProtKB: Large ribosomal subunit protein uL23

+
Macromolecule #40: 50S ribosomal protein L24

Macromolecule	Name: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 11.316205 KDa
Sequence	String: HIKKGDNVKV IAGKDKGKEG KVIATLPKKD RVVVEGVNIM KKHQKPTQLN PEGGILETEA AIHVSNVQLL DPKTNEPTRV GYKFVDGKK VRIAKKSGEE IKSN UniProtKB: Large ribosomal subunit protein uL24

+
Macromolecule #41: 50S ribosomal protein L25

Macromolecule	Name: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 10.4622 KDa
Sequence	String: ASLKSIIRQG KQTRSDLKQL RKSGKVPAVV YGYGTKNVSV KVDEVEFIKV IREVGRNGVI ELGVGSKTIK VMVADYQFDP LKNQITHID FLAIN UniProtKB: Large ribosomal subunit protein bL25

+
Macromolecule #42: 50S ribosomal protein L27

Macromolecule	Name: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 8.595688 KDa
Sequence	String: VSSTKNGRDS ESKRLGAKRA DGQFVTGGSI LYRQRGTKIY PGENVGRGGD DTLFAKIDGV VKFERKGRDK KQVSVYAVA UniProtKB: Large ribosomal subunit protein bL27

+
Macromolecule #43: 50S ribosomal protein L28

Macromolecule	Name: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 5.499432 KDa
Sequence	String: ASTGNRRSHA LNSTKRRWNA NLQKVRILVD GKPKKVWVSA RALKSGKVT UniProtKB: Large ribosomal subunit protein bL28

+
Macromolecule #44: 50S ribosomal protein L29

Macromolecule	Name: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 7.845942 KDa
Sequence	String: KAKEIRDLTT SEIEEQIKSS KEELFNLRFQ LATGQLEETA RIRTVRKTIA RLKTVARERE IEQSKAN UniProtKB: Large ribosomal subunit protein uL29

+
Macromolecule #45: 50S ribosomalprotein L32p

Macromolecule	Name: 50S ribosomalprotein L32p / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 5.406392 KDa
Sequence	String: AVPKRRTSKT RKNKRRTHFK ISVPGMTECP NCGEYKLSHR VCKNCGS UniProtKB: UNIPROTKB: A0A6K0ZQ16

+
Macromolecule #46: 50S ribosomal protein L5

Macromolecule	Name: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Staphylococcus aureus (bacteria)
Molecular weight	Theoretical: 17.651479 KDa
Sequence	String: KFNYSSVMEV PKIDKIVVNM GVGDAVQNSK VLDNAVEELE LITGQKPLVT KAKKSIATFR LREGMPIGAK VTLRGERMYE FLDKLISVS LPRVRDFQGV SKKAFDGRGN YTLGVKEQLI FPEIDYDKVS KVRGMDIVIV TTANTDEEAR ELLANFGMP UniProtKB: 50S ribosomal protein L5

-
Experimental details

-
Structure determination

Method	cryo EM
Processing	single particle reconstruction
Aggregation state	particle

-
Sample preparation

Buffer	pH: 7.6
Grid	Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 200
Vitrification	Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

-
Electron microscopy

Microscope	FEI TITAN KRIOS
Electron beam	Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron optics	Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recording	Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 47.0 e/Å²
Experimental equipment	Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Particle selection	Number selected: 383752
Startup model	Type of model: PDB ENTRY
Initial angle assignment	Type: NOT APPLICABLE
Final angle assignment	Type: NOT APPLICABLE
Final reconstruction	Resolution.type: BY AUTHOR / Resolution: 2.86 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 192105

+
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-Supplemental data

-Additional map: #1

-Sample components

+Entire : 70S

+Supramolecule #1: 70S

+Macromolecule #1: 23S rRNA

+Macromolecule #2: 16S rRNA

+Macromolecule #3: 5S rRNA

+Macromolecule #4: 50S ribosomal protein L33,50S ribosomal protein L30

+Macromolecule #5: 50S ribosomal protein L34

+Macromolecule #6: 50S ribosomal protein L35

+Macromolecule #7: 50S ribosomal protein L36

+Macromolecule #8: 30S ribosomal protein S10

+Macromolecule #9: 30S ribosomal protein S11

+Macromolecule #10: 30S ribosomal protein S12

+Macromolecule #11: 50S ribosomal protein L2

+Macromolecule #12: 30S ribosomal protein S13

+Macromolecule #13: 50S ribosomal protein L3

+Macromolecule #14: 50S ribosomal protein L4

+Macromolecule #15: 30S ribosomal protein S14 type Z

+Macromolecule #16: 30S ribosomal protein S15

+Macromolecule #17: 30S ribosomal protein S16

+Macromolecule #18: 50S ribosomal protein L6

+Macromolecule #19: 50S ribosomal protein L13

+Macromolecule #20: 30S ribosomal protein S17

+Macromolecule #21: 50S ribosomal protein L14

+Macromolecule #22: 30S ribosomal protein S18

+Macromolecule #23: 30S ribosomal protein S20

+Macromolecule #24: 50S ribosomal protein L15

+Macromolecule #25: 30S ribosomal protein S3

+Macromolecule #26: 50S ribosomal protein L16

+Macromolecule #27: 50S ribosomal protein L17

+Macromolecule #28: 30S ribosomal protein S4

+Macromolecule #29: 30S ribosomal protein S5

+Macromolecule #30: 50S ribosomal protein L18

+Macromolecule #31: 30S ribosomal protein S6

+Macromolecule #32: 50S ribosomal protein L19

+Macromolecule #33: 30S ribosomal protein S8

+Macromolecule #34: 50S ribosomal protein L20

+Macromolecule #35: 30S ribosomal protein S7

+Macromolecule #36: 50S ribosomal protein L21

+Macromolecule #37: 30S ribosomal protein S9

+Macromolecule #38: 50S ribosomal protein L22

+Macromolecule #39: 50S ribosomal protein L23

+Macromolecule #40: 50S ribosomal protein L24

+Macromolecule #41: 50S ribosomal protein L25

+Macromolecule #42: 50S ribosomal protein L27

+Macromolecule #43: 50S ribosomal protein L28

+Macromolecule #44: 50S ribosomal protein L29

+Macromolecule #45: 50S ribosomalprotein L32p

+Macromolecule #46: 50S ribosomal protein L5

-Experimental details

-Structure determination

-Sample preparation

-Electron microscopy

-Image processing

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