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- PDB-7asn: Staphylococcus aureus 50S after 30 minutes incubation a 37C -

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Basic information

Entry
Database: PDB / ID: 7asn
TitleStaphylococcus aureus 50S after 30 minutes incubation a 37C
Components
  • (50S ribosomal protein ...) x 23
  • 23S
  • 5S
KeywordsRIBOSOME / H68 / translation / protein synthesis
Function / homology
Function and homology information


large ribosomal subunit / 5S rRNA binding / transferase activity / ribosomal large subunit assembly / cytosolic large ribosomal subunit / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation ...large ribosomal subunit / 5S rRNA binding / transferase activity / ribosomal large subunit assembly / cytosolic large ribosomal subunit / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / cytoplasm
Similarity search - Function
Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / : / : / Ribosomal protein L16 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal L25p family ...Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / : / : / Ribosomal protein L16 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L32p, bacterial type / : / : / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L20 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L30, bacterial-type / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 superfamily / Ribosomal protein L16 / L28p-like / Ribosomal protein L20 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L21 / Ribosomal protein L19 / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal proteins 50S L24/mitochondrial 39S L24 / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal L32p protein family / Ribosomal protein L32p / Ribosomal protein L24 / Ribosomal protein L34 / Ribosomal protein L34 / Ribosomal protein L13, bacterial-type / Ribosomal protein L3, bacterial/organelle-type / Ribosomal protein L15, bacterial-type / 50S ribosomal protein uL4 / Ribosomal protein L2 signature. / Ribosomal protein L15, conserved site / Ribosomal protein L15 signature. / Ribosomal protein L2, conserved site / : / Ribosomal protein L10e/L16 / Ribosomal protein L10e/L16 superfamily / Ribosomal protein L16p/L10e / Ribosomal protein L13 signature. / Ribosomal protein L2, domain 3 / Ribosomal protein L13, conserved site / Ribosomal protein L14P, conserved site / Ribosomal protein L14 signature. / Ribosomal L29 protein / Ribosomal protein L29/L35 / Ribosomal protein L29/L35 superfamily / Ribosomal Proteins L2, C-terminal domain / Ribosomal protein L2, C-terminal / Ribosomal Proteins L2, C-terminal domain / Ribosomal protein L24 signature. / Ribosomal Proteins L2, RNA binding domain / Ribosomal Proteins L2, RNA binding domain / Ribosomal Proteins L2, RNA binding domain / Ribosomal protein L24/L26, conserved site / Ribosomal protein L2 / KOW (Kyprides, Ouzounis, Woese) motif. / Ribosomal protein L13 / Ribosomal protein L13 / Ribosomal protein L13 superfamily / Ribosomal protein L30, ferredoxin-like fold domain / Ribosomal protein L30p/L7e / Ribosomal protein L23
Similarity search - Domain/homology
RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein bL19 ...RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein bL25 / 50S ribosomal protein L32 / : / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL30
Similarity search - Component
Biological speciesStaphylococcus aureus (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.73 Å
AuthorsCimicata, G. / Bashan, A. / Yonath, A.
Funding support1items
OrganizationGrant numberCountry
European Research Council (ERC)322581
CitationJournal: To Be Published
Title: Staphylococcus aureus 50S after 30 minutes incubation a 37C
Authors: Cimicata, G. / Bashan, A. / Yonath, A.
History
DepositionOct 27, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Nov 17, 2021Provider: repository / Type: Initial release
Revision 1.1Oct 23, 2024Group: Data collection / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / em_admin / pdbx_entry_details / pdbx_modification_feature
Item: _em_admin.last_update / _pdbx_entry_details.has_protein_modification

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Structure visualization

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  • Deposited structure unit
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  • Superimposition on EM map
  • EMDB-11901
  • Imaged by UCSF Chimera
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Structure viewerMolecule:
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Assembly

Deposited unit
A: 23S
B: 5S
1: 50S ribosomal protein L33
2: 50S ribosomal protein L34
3: 50S ribosomal protein L35
F: 50S ribosomal protein L2
D: 50S ribosomal protein L3
E: 50S ribosomal protein L4
H: 50S ribosomal protein L13
L: 50S ribosomal protein L15
Y: 50S ribosomal protein L16
G: 50S ribosomal protein L14
M: 50S ribosomal protein L18
N: 50S ribosomal protein L19
O: 50S ribosomal protein L20
P: 50S ribosomal protein L21
Q: 50S ribosomal protein L22
R: 50S ribosomal protein L23
S: 50S ribosomal protein L24
T: 50S ribosomal protein L25
a: 50S ribosomal protein L27
V: 50S ribosomal protein L28
W: 50S ribosomal protein L29
X: 50S ribosomal protein L30
b: 50S ribosomal protein L32


Theoretical massNumber of molelcules
Total (without water)1,205,93025
Polymers1,205,93025
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: microscopy
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area123120 Å2
ΔGint-1068 kcal/mol
Surface area470710 Å2
MethodPISA

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Components

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RNA chain , 2 types, 2 molecules AB

#1: RNA chain 23S


Mass: 888200.938 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: ...Details: GAUUAAGUUAUUAAGGGCGCACGGUGGAUGCCUUGGCACUAGAAGCCGAUGAAGGACGUUACUAACGACGAUAUGCUUUGGGGAGCUGUAAGCUUUGAUCCAGAGAUUUCCGAAUGGGGAAACCCAGCAUGAGUUAUGUCAUGUUAUCGAUAUGUGAAUACAUAGCAUAUCAGAAGGCACACCCGGAGAACUGAAACAUCUUAGUACCCGGAGGAAGAGAAAGAAAAUUCGAUUCCCUUAGUAGCGGCGAGCGAAACGGGAAGAGCCCAAACCAACAAGCUUGCUUGUUGGGGUUGUAGGACACUCUGUACGGAGUUACAAAGGACGACAUUAGACGAAUCAUCUGGAAAGAUGAAUCAAAGAAGGUAAUAAUCCUGUAGUCGAAAAUGUUGUCUCUCUUGAGUGGAUCCUGAGUACGACGGAGCACGUGAAAUUCCGUCGGAAUCUGGGAGGACCAUCUCCUAAGGCUAAAUACUCUCUAGUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGUGAAAAGCACCCCGGAAGGGGAGUGAAAUAGAACCUGAAACCGUGUGCUUACAAGUAGUCAGAGCCCGUUAAUGGGUGAUGGCGUGCCUUUUGUAGAAUGAACCGGCGAGUUACGAUUUGAUGCAAGGUUAAGCAGUAAAUGUGGAGCCGUAGCGAAAGCGAGUCUGAAUAGGGCGUUUAGUAUUUGGUCGUAGACCCGAAACCAGGUGAUCUACCCUUGGUCAGGUUGAAGUUCAGGUAACACUGAAUGGAGGACCGAACCGACUUACGUUGAAAAGUGAGCGGAUGAACUGAGGGUAGCGGAGAAAUUCCAAUCGAACCUGGAGAUAGCUGGUUCUCUCCGAAAUAGCUUUAGGGCUAGCCUCAAGUGAUGAUUAUUGGAGGUAGAGCACUGUUUGGACGAGGGGCCCCUCUCGGGUUACCGAAUUCAGACAAACUCCGAAUGCCAAUUAAUUUAACUUGGGAGUCAGAACAUGGGUGAUAAGGUCCGUGUUCGAAAGGGAAACAGCCCAGACCACCAGCUAAGGUCCCAAAAUAUAUGUUAAGUGGAAAAGGAUGUGGCGUUGCCCAGACAACUAGGAUGUUGGCUUAGAAGCAGCCAUCAUUUAAAGAGUGCGUAAUAGCUCACUAGUCGAGUGACACUGCGCCGAAAAUGUACCGGGGCUAAACAUAUUACCGAAGCUGUGGAUUGUCCUUUGGACAAUGGUAGGAGAGCGUUCUAAGGGCGUUGAAGCAUGAUCGUAAGGACAUGUGGAGCGCUUAGAAGUGAGAAUGCCGGUGUGAGUAGCGAAAGACGGGUGAGAAUCCCGUCCACCGAUUGACUAAGGUUUCCAGAGGAAGGCUCGUCCGCUCUGGGUUAGUCGGGUCCUAAGCUGAGGCCGACAGGCGUAGGCGAUGGAUAACAGGUUGAUAUUCCUGUACCACCUAUAAUCGUUUUAAUCGAUGGGGGGACGCAGUAGGAUAGGCGAAGCGUGCGAUUGGAUUGCACGUCUAAGCAGUAAGGCUGAGUAUUAGGCAAAUCCGGUACUCGUUAAGGCUGAGCUGUGAUGGGGAGAAGACAUUGAGUCUUCGAGUCGUUGAUUUCACACUGCCGAGAAAAGCCUCUAGAUAGAAAAUAGGUGCCCGUACCGCAAACCGACACAGGUAGUCAAGAUGAGAAUUCUAAGGUGAGCGAGCGAACUCUCGUUAAGGAACUCGGCAAAAUGACCCCGGUAACUUCGGGAGAAGGGGUGCUCUUUAGGGUUAACGCCCAGAAGAGCCGCAGUGAAUAGGCCCAAGCGACUGUUUAUCAAAAACACAGGUCUCUGCUAAACCGUAAGGUGAUGUAUAGGGGCUGACGCCUGCCCGGUGCUGGAAGGUUAAGAGGAGUGGUUAGCUUCUGCGAAGCUACGAAUCGAAGCCCCAGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGAAAGGCGUAACGAUUUGGGCACUGUCUCAACGAGAGACUCGGUGAAAUCAUAGUACCUGUGAAGAUGCAGGUUACCCGCGACAGGACGGAAAGACCCCGUGGAGCUUUACUGUAGCCUGAUAUUGAAAUUCGGCACAGCUUGUACAGGAUAGGUAGGAGCCUUUGAAACGUGAGCGCUAGCUUACGUGGAGGCGCUGGUGGGAUACUACCCUAGCUGUGUUGGCUUUCUAACCCGCACCACUUAUCGUGGUGGGAGACAGUGUCAAGCGGGCAGUUUGACUGGGGCGGUCGCCUCCUAAAAGGUAACGGAGGCGCUCAAAGGUUCCCUCAGAAUGGUUGGAAAUCAUUCAUAGAGUGUAAAGGCAUAAGGGAGCUUGACUGCGAGACCUACAAGUCGAGCAGGGUCGAAAGACGGACUUAGUGAUCCGGUGGUUCCGCAUGGAAGGGCCAUCGCUCAACGGAUAAAAGCUACCCCGGGGAUAACAGGCUUAUCUCCCCCAAGAGUUCACAUCGACGGGGAGGUUUGGCACCUCGAUGUCGGCUCAUCGCAUCCUGGGGCUGUAGUCGGUCCCAAGGGUUGGGCUGUUCGCCCAUUAAAGCGGUACGCGAGCUGGGUUCAGAACGUCGUGAGACAGUUCGGUCCCUAUCCGUCGUGGGCGUAGGAAAUUUGAGAGGAGCUGUCCUUAGUACGAGAGGACCGGGAUGGACAUACCUCUGGUGUACCAGUUGUCGUGCCAACGGCAUAGCUGGGUAGCUAUGUGUGGACGGGAUAAGUGCUGAAAGCAUCUAAGCAUGAAGCCCCCCUCAAGAUGAGAUUUCCCAACUUCGGUUAUAAGAUCCCUCAAAGAUGAUGAGGUUAAUAGGUUCGAGGUGGAAGCAUGGUGACAUGUGGAGCUGACGAAUACUAAUCGAUCGAAGACUUAAUCAA
Source: (gene. exp.) Staphylococcus aureus (bacteria) / Production host: Staphylococcus aureus (bacteria)
#2: RNA chain 5S


Mass: 34120.289 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: UCUGGUGACUAUAGCAAGGAGGUCACACCUGUUCCCAUGCCGAACACAGAAGUUAAGGUCUUUAGCGACGAUGGUAGCCAACUUACGUUCCGCUAGAGUAGAACGUUGCCAGGC
Source: (gene. exp.) Staphylococcus aureus (bacteria) / Production host: Staphylococcus aureus (bacteria)

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50S ribosomal protein ... , 23 types, 23 molecules 123FDEHLYGMNOPQRSTaVWXb

#3: Protein/peptide 50S ribosomal protein L33


Mass: 5684.561 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rpmG2, rpmG, rpmGA, BN1321_250027, BSZ10_06805, BTN44_05930, CSC83_10520, CSC87_10725, CV021_11035, EP54_12360, EQ90_07500, ERS072840_02602, ERS140147_01724, HMPREF3211_00887, RK64_07170, SAMEA2445518_00543
Production host: Staphylococcus aureus (bacteria) / References: UniProt: A0A077V2P0
#4: Protein/peptide 50S ribosomal protein L34


Mass: 5252.369 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rpmH, rpmH_1, BN1321_430090, BSZ10_04010, BTN44_00005, C7P97_05575, CSC83_09170, CSC87_00805, CV021_04430, D1G01_01950, D1G05_00255, D1G09_00255, D1G21_10375, EF900_09815, EP54_05535, EQ90_ ...Gene: rpmH, rpmH_1, BN1321_430090, BSZ10_04010, BTN44_00005, C7P97_05575, CSC83_09170, CSC87_00805, CV021_04430, D1G01_01950, D1G05_00255, D1G09_00255, D1G21_10375, EF900_09815, EP54_05535, EQ90_05835, ERS072840_02076, ERS140147_01157, HMPREF3211_01016, M1K003_1448, NCTC10654_02887, NCTC10702_04202, NCTC13131_00239, NCTC5661_02478, NCTC5664_00960, NCTC6133_03671, NCTC7878_00992, NCTC7887_02502, NCTC7988_02787, RK64_00675, SAMEA1708664_00264, SAMEA1708674_02331, SAMEA2445518_01774
Production host: Staphylococcus aureus (bacteria) / References: UniProt: W8TQ59
#5: Protein 50S ribosomal protein L35


Mass: 7461.992 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rpmI, BJQ98_01270, BKL65_00680, BN1321_260310, BN1326_80212, BO217_1935, BVV32_04930, BZP34_02055, ERS072738_01466, ERS072840_00232, ERS073071_00087, ERS073147_00101, ERS073583_00087, ERS074020_ ...Gene: rpmI, BJQ98_01270, BKL65_00680, BN1321_260310, BN1326_80212, BO217_1935, BVV32_04930, BZP34_02055, ERS072738_01466, ERS072840_00232, ERS073071_00087, ERS073147_00101, ERS073583_00087, ERS074020_02606, ERS140147_00900, HMPREF3211_00281, SAMEA2445518_00211, SAMEA3448837_00215
Production host: Staphylococcus aureus (bacteria) / References: UniProt: A0A077UL47
#6: Protein 50S ribosomal protein L2


Mass: 29827.607 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria) / Gene: rap, rplB / Production host: Staphylococcus aureus (bacteria) / References: UniProt: B3VKN3
#7: Protein 50S ribosomal protein L3


Mass: 23199.545 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rplC, rplC_1, BN1321_380082, BTN44_13670, C7P97_04145, CSC83_04430, CSC87_02210, CV021_17915, D1G01_09415, D1G05_10655, D1G09_02825, D1G21_09885, EP54_02075, EQ90_01290, ERS072840_01156, ...Gene: rplC, rplC_1, BN1321_380082, BTN44_13670, C7P97_04145, CSC83_04430, CSC87_02210, CV021_17915, D1G01_09415, D1G05_10655, D1G09_02825, D1G21_09885, EP54_02075, EQ90_01290, ERS072840_01156, ERS140147_00638, HMPREF3211_01575, M1K003_0793, NCTC10654_02422, NCTC10702_03548, NCTC13131_01593, NCTC5664_01662, NCTC6133_03040, NCTC7878_01008, NCTC7988_02329, RK64_11980, SAMEA2445518_02252
Production host: Staphylococcus aureus (bacteria) / References: UniProt: W8U3W0
#8: Protein 50S ribosomal protein L4


Mass: 22364.504 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rplD, rplD_1, BN1321_380081, BTN44_13665, C7P97_04140, CSC83_04425, CSC87_02215, CV021_17920, D1G01_09420, D1G05_10660, D1G09_02830, D1G21_09890, EF900_08505, EP54_02080, EQ90_01285, HMPREF3211_ ...Gene: rplD, rplD_1, BN1321_380081, BTN44_13665, C7P97_04140, CSC83_04425, CSC87_02215, CV021_17920, D1G01_09420, D1G05_10660, D1G09_02830, D1G21_09890, EF900_08505, EP54_02080, EQ90_01285, HMPREF3211_01574, M1K003_0792, NCTC10654_02421, NCTC10702_03547, NCTC13131_01592, NCTC6133_03039, NCTC7988_02328, RK64_11975, SAMEA1708674_02412
Production host: Staphylococcus aureus (bacteria) / References: UniProt: W8TRC7
#9: Protein 50S ribosomal protein L13


Mass: 16202.232 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVTYTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLIENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rplM, BN1321_380050, BTN44_13510, C7P97_03985, DD547_02255, DDL17_01300, DQV20_00425, DQV53_12855, E3A28_11285, E3K14_11845, EP54_02235, EQ90_01130, ERS072840_02335, ERS140147_00605, FA040_ ...Gene: rplM, BN1321_380050, BTN44_13510, C7P97_03985, DD547_02255, DDL17_01300, DQV20_00425, DQV53_12855, E3A28_11285, E3K14_11845, EP54_02235, EQ90_01130, ERS072840_02335, ERS140147_00605, FA040_01450, G0V24_08140, G0X12_08225, G0X27_07160, G0Z08_03675, G0Z18_07935, G6W67_02325, G6W97_08495, G6X24_00060, G6X31_08745, G6X35_06870, G6Y10_08530, G6Y24_08740, G6Y28_07645, G6Y30_00220, GF545_10180, GF559_05955, GIX97_07485, GO677_03530, GO706_12960, GO746_00955, GO788_09495, GO793_16575, GO803_10595, GO805_15105, GO810_02220, GO821_04070, GO894_08805, GO915_09285, GO941_09170, GO942_00990, HMPREF3211_01543, M1K003_0759, NCTC10654_02389, NCTC5664_01699, NCTC6133_03005, NCTC7878_01045, NCTC9944_02373, RK64_11820, SAMEA1029528_02041, SAMEA1029547_01439, SAMEA1029553_01089, SAMEA1469884_02397, SAMEA1531680_02385, SAMEA1531701_02555, SAMEA1964876_00455, SAMEA1965205_00275, SAMEA1966505_00275, SAMEA1969349_01203, SAMEA1969845_00716, SAMEA1971706_00637, SAMEA1972827_01250, SAMEA2076212_00390, SAMEA2076218_01251, SAMEA2076220_01014, SAMEA2076226_01596, SAMEA2076463_01028, SAMEA2076464_01027, SAMEA2076470_00622, SAMEA2076472_00582, SAMEA2076478_00675, SAMEA2076480_00937, SAMEA2076481_01590, SAMEA2076743_01216, SAMEA2076745_00724, SAMEA2076746_01275, SAMEA2076747_00950, SAMEA2076749_00584, SAMEA2076751_00053, SAMEA2076752_01109, SAMEA2076755_00583, SAMEA2076756_00903, SAMEA2076758_00714, SAMEA2076759_00275, SAMEA2076761_00275, SAMEA2076762_00704, SAMEA2076763_01332, SAMEA2076764_00518, SAMEA2076765_02012, SAMEA2077023_00741, SAMEA2077025_00275, SAMEA2077027_00275, SAMEA2077029_00034, SAMEA2077031_00035, SAMEA2077034_00275, SAMEA2077035_01581, SAMEA2077039_01078, SAMEA2077040_01563, SAMEA2077041_00517, SAMEA2077044_00857, SAMEA2077045_00275, SAMEA2077046_00036, SAMEA2077293_00052, SAMEA2077294_01342, SAMEA2077295_00053, SAMEA2077297_01126, SAMEA2077300_00275, SAMEA2077301_00052, SAMEA2077302_00053, SAMEA2077303_00275, SAMEA2077307_00275, SAMEA2077832_00052, SAMEA2078252_00274, SAMEA2078256_00883, SAMEA2078307_00275, SAMEA2078308_01023, SAMEA2078553_01490, SAMEA2078558_01230, SAMEA2078560_01652, SAMEA2078569_00357, SAMEA2078570_00052, SAMEA2078572_00275, SAMEA2078824_01027, SAMEA2078837_00054, SAMEA2079048_00274, SAMEA2079051_00455, SAMEA2079277_00790, SAMEA2079291_00654, SAMEA2079503_01074, SAMEA2079507_00738, SAMEA2079512_00985, SAMEA2079517_00807, SAMEA2079724_01580, SAMEA2079727_01944, SAMEA2079728_01700, SAMEA2079732_01390, SAMEA2079742_00609, SAMEA2079946_01222, SAMEA2079949_01552, SAMEA2079951_01612, SAMEA2079952_01663, SAMEA2079957_01685, SAMEA2079958_01389, SAMEA2079960_00127, SAMEA2079961_00786, SAMEA2079968_01564, SAMEA2080329_01800, SAMEA2080330_00708, SAMEA2080334_00548, SAMEA2080433_01339, SAMEA2080812_01355, SAMEA2080898_00053, SAMEA2080900_01196, SAMEA2080904_00343, SAMEA2080913_00053, SAMEA2081043_00554, SAMEA2081053_00274, SAMEA2081054_01328, SAMEA2081055_00275, SAMEA2081060_00275, SAMEA2081211_00477, SAMEA2081213_00805, SAMEA2081218_01795, SAMEA2081341_01673, SAMEA2081342_01345, SAMEA2081349_01639, SAMEA2081359_01659, SAMEA2081362_00052, SAMEA2081468_01442, SAMEA2081474_01226, SAMEA2081475_01016, SAMEA2081476_01006, SAMEA2081479_00804, SAMEA2081480_00055, SAMEA2081560_00455, SAMEA2081561_00342, SAMEA2081564_00035, SAMEA2081567_00275, SAMEA2081568_00053, SAMEA2081569_01639, SAMEA2081570_00037, SAMEA2081571_00035, SAMEA2081572_00455, SAMEA2081573_01387, SAMEA2081575_01314, SAMEA2081577_01322, SAMEA2081578_01005, SAMEA2081579_00052, SAMEA2081581_00454, SAMEA2081582_00712, SAMEA2081673_01788, SAMEA2081674_01291, SAMEA2445518_02285, SAMEA958766_01482, SAMEA958770_01502, SAMEA958772_01700, SAMEA958778_01191, SAMEA958779_00429, SAMEA958785_01487, SAMEA958793_01527, SAMEA958798_00724, SAMEA958804_01654, SAMEA958810_01120, SAMEA958836_01760, SAMEA958838_00421, SAMEA958845_01459, SAMEA958846_01156, SAMEA958848_00445, SAMEA958855_00819, SAMEA958858_01763, SAMEA958870_01857, SAMEA958898_00142, SAMEA958906_00686, SAMEA958924_01503, SAMEA958925_01962, SAMEA958951_01812, SAMEA958953_01734, SAMEA958961_01377, SAMEA958979_01446, SAMEA958987_00840, SAMEA958995_01395, SAST44_02479, SAST45_02461
Production host: Staphylococcus aureus (bacteria) / References: UniProt: W8TUE6
#10: Protein 50S ribosomal protein L15


Mass: 15628.890 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rplO, AYM28_12445, AYM37_12445, BMF23_12440, BN1321_380063, BN1326_140024, BO217_1005, ERS072738_00725, ERS072840_01137, ERS073071_00328, ERS073583_01088, ERS074020_00479, ERS140147_00619, ...Gene: rplO, AYM28_12445, AYM37_12445, BMF23_12440, BN1321_380063, BN1326_140024, BO217_1005, ERS072738_00725, ERS072840_01137, ERS073071_00328, ERS073583_01088, ERS074020_00479, ERS140147_00619, HMPREF3211_01556, SAMEA2445518_02271
Production host: Staphylococcus aureus (bacteria) / References: UniProt: A0A077UGA7
#11: Protein 50S ribosomal protein L16


Mass: 15599.435 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rplP, rplP_1, BN1321_380075, BTN44_13635, C7P97_04110, CSC83_04395, CSC87_02245, CV021_17950, D1G01_09450, D1G05_10690, D1G09_02860, D1G21_09920, EF900_08535, EP54_02110, EQ90_01255, ERS072840_ ...Gene: rplP, rplP_1, BN1321_380075, BTN44_13635, C7P97_04110, CSC83_04395, CSC87_02245, CV021_17950, D1G01_09450, D1G05_10690, D1G09_02860, D1G21_09920, EF900_08535, EP54_02110, EQ90_01255, ERS072840_01149, ERS140147_00631, HMPREF3211_01568, M1K003_0786, NCTC10654_02415, NCTC10702_03541, NCTC13131_01586, NCTC5664_01669, NCTC6133_03033, NCTC7878_01016, NCTC7988_02322, RK64_11945, SAMEA1708664_00133, SAMEA1708674_02418, SAMEA2445518_02259
Production host: Staphylococcus aureus (bacteria) / References: UniProt: A0A077V4G0
#12: Protein 50S ribosomal protein L14


Mass: 13157.342 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rplN, rplNA, AYM28_12490, AYM37_12490, BMF23_12485, BN1321_380072, BN1326_140033, BO217_0996, ERS072738_00716, ERS072840_01146, ERS073071_00337, ERS073583_01097, ERS074020_00488, ERS140147_ ...Gene: rplN, rplNA, AYM28_12490, AYM37_12490, BMF23_12485, BN1321_380072, BN1326_140033, BO217_0996, ERS072738_00716, ERS072840_01146, ERS073071_00337, ERS073583_01097, ERS074020_00488, ERS140147_00628, HMPREF3211_01565, SAMEA2445518_02262
Production host: Staphylococcus aureus (bacteria) / References: UniProt: A0A077UUA0
#13: Protein 50S ribosomal protein L18


Mass: 12879.740 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rplR, BN1321_380066, BTN44_13590, C7P97_04065, CSC87_02290, DD547_02271, DDL17_01380, DQV20_00505, DQV53_12935, E3A28_11205, E3K14_11925, EP54_02155, EQ90_01210, ERS072840_01140, FA040_01530, ...Gene: rplR, BN1321_380066, BTN44_13590, C7P97_04065, CSC87_02290, DD547_02271, DDL17_01380, DQV20_00505, DQV53_12935, E3A28_11205, E3K14_11925, EP54_02155, EQ90_01210, ERS072840_01140, FA040_01530, FVP29_08980, G0V24_08060, G0X12_08305, G0X27_07080, G0Y02_02885, G0Z18_08015, G6W67_02245, G6W97_08415, G6X24_00140, G6X31_08665, G6X35_06950, G6Y10_08450, G6Y24_08820, G6Y28_10990, G6Y30_07090, GF545_07570, GF559_06035, GIX97_07565, GO677_05785, GO706_04040, GO746_01035, GO788_09415, GO793_01695, GO803_10675, GO805_15025, GO810_02140, GO821_04150, GO894_08885, GO915_00080, GO941_09030, GO942_01070, HMPREF3211_01559, M1K003_0777, NCTC10654_02406, NCTC5664_01678, NCTC6133_03024, NCTC9944_02390, RK64_11900, SAMEA1029528_02024, SAMEA1029547_01422, SAMEA1029553_01106, SAMEA1469856_00806, SAMEA1469884_01240, SAMEA1531680_01581, SAMEA1531701_01522, SAMEA1964876_00438, SAMEA1965205_00258, SAMEA1966505_00258, SAMEA1969349_01220, SAMEA1969845_00699, SAMEA1971706_00620, SAMEA1972827_01267, SAMEA2076212_00373, SAMEA2076218_01268, SAMEA2076220_00997, SAMEA2076226_01613, SAMEA2076463_01011, SAMEA2076464_01010, SAMEA2076470_00639, SAMEA2076472_00565, SAMEA2076478_00692, SAMEA2076480_00954, SAMEA2076481_01607, SAMEA2076743_01233, SAMEA2076745_00707, SAMEA2076746_01292, SAMEA2076747_00967, SAMEA2076749_00567, SAMEA2076751_00070, SAMEA2076752_01092, SAMEA2076755_00566, SAMEA2076756_00920, SAMEA2076758_00731, SAMEA2076759_00258, SAMEA2076761_00258, SAMEA2076762_00687, SAMEA2076763_01349, SAMEA2076764_00535, SAMEA2076765_01995, SAMEA2077023_00724, SAMEA2077025_00258, SAMEA2077027_00258, SAMEA2077029_00051, SAMEA2077031_00052, SAMEA2077034_00258, SAMEA2077035_01598, SAMEA2077039_01095, SAMEA2077040_01546, SAMEA2077041_00534, SAMEA2077044_00840, SAMEA2077045_00258, SAMEA2077046_00053, SAMEA2077293_00069, SAMEA2077294_01359, SAMEA2077295_00070, SAMEA2077297_01109, SAMEA2077300_00258, SAMEA2077301_00069, SAMEA2077302_00070, SAMEA2077303_00258, SAMEA2077307_00258, SAMEA2077832_00069, SAMEA2078252_00257, SAMEA2078256_00866, SAMEA2078307_00258, SAMEA2078308_01006, SAMEA2078553_01507, SAMEA2078558_01213, SAMEA2078560_01669, SAMEA2078569_00374, SAMEA2078570_00069, SAMEA2078572_00258, SAMEA2078824_01010, SAMEA2078837_00071, SAMEA2079048_00257, SAMEA2079051_00438, SAMEA2079277_00773, SAMEA2079291_00671, SAMEA2079503_01091, SAMEA2079507_00721, SAMEA2079512_01002, SAMEA2079517_00790, SAMEA2079724_01597, SAMEA2079727_01927, SAMEA2079728_01683, SAMEA2079732_01373, SAMEA2079742_00592, SAMEA2079946_01239, SAMEA2079949_01535, SAMEA2079951_01629, SAMEA2079952_01680, SAMEA2079957_01668, SAMEA2079958_01372, SAMEA2079960_00144, SAMEA2079961_00803, SAMEA2079968_01581, SAMEA2080329_01783, SAMEA2080330_00725, SAMEA2080334_00565, SAMEA2080433_01356, SAMEA2080812_01372, SAMEA2080898_00070, SAMEA2080900_01213, SAMEA2080904_00360, SAMEA2080913_00070, SAMEA2081043_00537, SAMEA2081053_00257, SAMEA2081054_01345, SAMEA2081055_00258, SAMEA2081060_00258, SAMEA2081211_00494, SAMEA2081213_00788, SAMEA2081218_01778, SAMEA2081341_01656, SAMEA2081342_01328, SAMEA2081349_01622, SAMEA2081359_01676, SAMEA2081362_00069, SAMEA2081468_01459, SAMEA2081474_01209, SAMEA2081475_01033, SAMEA2081476_01023, SAMEA2081479_00821, SAMEA2081480_00072, SAMEA2081560_00438, SAMEA2081561_00359, SAMEA2081564_00052, SAMEA2081567_00258, SAMEA2081568_00070, SAMEA2081569_01622, SAMEA2081570_00054, SAMEA2081571_00052, SAMEA2081572_00438, SAMEA2081573_01404, SAMEA2081575_01297, SAMEA2081577_01305, SAMEA2081578_00988, SAMEA2081579_00069, SAMEA2081581_00437, SAMEA2081582_00695, SAMEA2081673_01771, SAMEA2081674_01308, SAMEA958766_01465, SAMEA958770_01485, SAMEA958772_01683, SAMEA958778_01174, SAMEA958779_00446, SAMEA958785_01470, SAMEA958793_01510, SAMEA958798_00741, SAMEA958804_01671, SAMEA958810_01103, SAMEA958836_01743, SAMEA958838_00438, SAMEA958845_01442, SAMEA958846_01173, SAMEA958848_00428, SAMEA958855_00836, SAMEA958858_01746, SAMEA958870_00125, SAMEA958898_00125, SAMEA958906_00669, SAMEA958924_01520, SAMEA958925_01945, SAMEA958951_01795, SAMEA958953_01717, SAMEA958961_01394, SAMEA958979_01429, SAMEA958987_00857, SAMEA958995_01412, SAST44_02495, SAST45_02480
Production host: Staphylococcus aureus (bacteria) / References: UniProt: W8TRE0
#14: Protein 50S ribosomal protein L19


Mass: 13104.381 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQVFEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYLRSLRGKAARIQEIR
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rplS, BN1321_240087, C7P97_10140, CSC87_03990, DD547_01263, DQV53_06625, E3A28_02425, E3K14_06125, E4U00_03800, EP54_08610, EQ90_08050, ERS072840_01382, ERS140147_00145, FA040_04695, G0V24_ ...Gene: rplS, BN1321_240087, C7P97_10140, CSC87_03990, DD547_01263, DQV53_06625, E3A28_02425, E3K14_06125, E4U00_03800, EP54_08610, EQ90_08050, ERS072840_01382, ERS140147_00145, FA040_04695, G0V24_07190, G0X12_05090, G0Y02_06645, G0Z08_08030, G0Z18_11335, G6Y10_05730, GF545_05790, GF559_04760, GO677_10260, GO746_09515, GO788_03405, GO793_08790, GO803_14020, GO805_11845, GO821_05955, GO894_03270, GO915_11865, GO941_12700, GO942_07360, HMPREF3211_01285, NCTC10654_01285, NCTC10702_01957, NCTC5664_02266, NCTC6133_01508, NCTC7878_01877, NCTC9944_01245, RK64_06615, SAMEA1029528_00180, SAMEA1029547_01639, SAMEA1029553_01694, SAMEA1469856_01649, SAMEA1469884_01653, SAMEA1531680_01523, SAMEA1531701_01424, SAMEA1964876_01594, SAMEA1965205_01564, SAMEA1966505_01051, SAMEA1969349_01580, SAMEA1969845_01317, SAMEA1971706_01742, SAMEA1972827_01165, SAMEA2076212_01561, SAMEA2076218_01166, SAMEA2076220_01595, SAMEA2076226_00704, SAMEA2076463_01302, SAMEA2076464_01301, SAMEA2076470_01521, SAMEA2076472_01630, SAMEA2076478_01657, SAMEA2076480_01313, SAMEA2076481_01967, SAMEA2076743_01594, SAMEA2076745_01922, SAMEA2076746_01652, SAMEA2076747_01313, SAMEA2076749_01638, SAMEA2076751_01449, SAMEA2076752_01398, SAMEA2076755_01205, SAMEA2076756_01265, SAMEA2076758_01105, SAMEA2076759_01221, SAMEA2076761_01573, SAMEA2076762_01748, SAMEA2076763_01680, SAMEA2076764_01739, SAMEA2076765_01744, SAMEA2077023_01751, SAMEA2077025_01475, SAMEA2077027_01540, SAMEA2077029_01471, SAMEA2077031_01603, SAMEA2077034_01711, SAMEA2077035_01319, SAMEA2077039_01455, SAMEA2077040_01123, SAMEA2077041_01725, SAMEA2077044_01527, SAMEA2077045_01616, SAMEA2077046_01398, SAMEA2077293_01600, SAMEA2077294_01704, SAMEA2077295_01402, SAMEA2077297_01399, SAMEA2077300_01703, SAMEA2077301_01400, SAMEA2077302_01629, SAMEA2077303_01480, SAMEA2077307_01431, SAMEA2077832_01722, SAMEA2078252_01724, SAMEA2078256_01341, SAMEA2078307_01653, SAMEA2078308_01311, SAMEA2078553_01200, SAMEA2078558_01504, SAMEA2078560_01533, SAMEA2078569_01779, SAMEA2078570_01636, SAMEA2078572_01868, SAMEA2078824_01299, SAMEA2078837_01604, SAMEA2079048_01517, SAMEA2079051_01620, SAMEA2079277_01335, SAMEA2079291_01801, SAMEA2079503_01679, SAMEA2079507_01757, SAMEA2079512_01608, SAMEA2079517_01329, SAMEA2079724_01180, SAMEA2079727_00969, SAMEA2079728_01478, SAMEA2079732_01168, SAMEA2079742_01438, SAMEA2079946_01137, SAMEA2079949_01122, SAMEA2079951_01330, SAMEA2079952_01170, SAMEA2079957_01253, SAMEA2079958_01022, SAMEA2079960_01167, SAMEA2079961_00556, SAMEA2079968_01301, SAMEA2080329_01220, SAMEA2080330_01334, SAMEA2080334_01561, SAMEA2080433_01108, SAMEA2080812_01238, SAMEA2080898_02005, SAMEA2080900_01558, SAMEA2080904_01431, SAMEA2080913_01447, SAMEA2081043_01633, SAMEA2081053_01540, SAMEA2081054_01212, SAMEA2081055_01541, SAMEA2081060_00861, SAMEA2081211_01539, SAMEA2081213_01224, SAMEA2081218_01041, SAMEA2081341_01184, SAMEA2081342_00977, SAMEA2081349_01062, SAMEA2081359_01184, SAMEA2081362_01584, SAMEA2081468_01339, SAMEA2081474_01500, SAMEA2081475_01576, SAMEA2081476_00900, SAMEA2081479_01412, SAMEA2081480_01678, SAMEA2081560_01281, SAMEA2081561_01518, SAMEA2081564_01694, SAMEA2081567_01683, SAMEA2081568_01691, SAMEA2081569_01255, SAMEA2081570_01694, SAMEA2081571_01619, SAMEA2081572_01431, SAMEA2081573_01283, SAMEA2081575_01602, SAMEA2081577_01611, SAMEA2081578_01280, SAMEA2081579_01502, SAMEA2081581_01408, SAMEA2081582_01700, SAMEA2081673_01062, SAMEA2081674_01060, SAMEA2445518_00637, SAMEA958766_00811, SAMEA958770_01326, SAMEA958772_01210, SAMEA958778_00152, SAMEA958779_01245, SAMEA958785_00831, SAMEA958793_01036, SAMEA958798_01018, SAMEA958804_00839, SAMEA958810_01376, SAMEA958836_00285, SAMEA958838_00849, SAMEA958845_00961, SAMEA958846_01705, SAMEA958848_01729, SAMEA958855_00693, SAMEA958858_00386, SAMEA958870_02229, SAMEA958898_00775, SAMEA958906_01143, SAMEA958924_01238, SAMEA958951_01058, SAMEA958953_01088, SAMEA958961_01310, SAMEA958987_01455, SAMEA958995_00733, SAST44_01274, SAST45_01311
Production host: Staphylococcus aureus (bacteria) / References: UniProt: A0A077UVB6
#15: Protein 50S ribosomal protein L20


Mass: 13459.919 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rplT, BJQ98_01265, BKL65_00685, BN1321_260309, BN1326_80211, BO217_1936, BVV32_04935, BZP34_02060, ERS072738_01465, ERS072840_00233, ERS073071_00088, ERS073147_00102, ERS073583_00088, ERS074020_ ...Gene: rplT, BJQ98_01265, BKL65_00685, BN1321_260309, BN1326_80211, BO217_1936, BVV32_04935, BZP34_02060, ERS072738_01465, ERS072840_00233, ERS073071_00088, ERS073147_00102, ERS073583_00088, ERS074020_02607, ERS140147_00901, HMPREF3211_00282, SAMEA2445518_00212, SAMEA3448837_00216
Production host: Staphylococcus aureus (bacteria) / References: UniProt: A0A077VMP6
#16: Protein 50S ribosomal protein L21


Mass: 11354.081 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rplU, AYM28_09130, AYM37_09130, BMF23_03765, BN1321_260285, BN1326_80188, BO217_1960, ERS072840_00775, ERS073071_00111, ERS073583_00111, HMPREF3211_01799, SAMEA2445518_00236
Production host: Staphylococcus aureus (bacteria) / References: UniProt: D7URR3
#17: Protein 50S ribosomal protein L22


Mass: 12328.358 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria) / Gene: rplV, GO915_00135 / Production host: Staphylococcus aureus (bacteria) / References: UniProt: A0A6I4H7Y4
#18: Protein 50S ribosomal protein L23


Mass: 10380.100 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rplW, rplW_1, BN1321_380080, BTN44_13660, C7P97_04135, CSC83_04420, CSC87_02220, CV021_17925, D1G01_09425, D1G05_10665, D1G09_02835, D1G21_09895, EF900_08510, EP54_02085, EQ90_01280, ERS072840_ ...Gene: rplW, rplW_1, BN1321_380080, BTN44_13660, C7P97_04135, CSC83_04420, CSC87_02220, CV021_17925, D1G01_09425, D1G05_10665, D1G09_02835, D1G21_09895, EF900_08510, EP54_02085, EQ90_01280, ERS072840_01154, HMPREF3211_01573, M1K003_0791, NCTC10654_02420, NCTC10702_03546, NCTC13131_01591, NCTC6133_03038, NCTC7878_01010, NCTC7988_02327, RK64_11970, SAMEA1708664_00534, SAMEA1708674_02413
Production host: Staphylococcus aureus (bacteria) / References: UniProt: W8TUB4
#19: Protein 50S ribosomal protein L24


Mass: 11316.205 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rplX, rplX_1, BN1321_380071, BTN44_13615, C7P97_04090, CSC83_04375, CSC87_02265, CV021_17970, D1G01_09470, D1G05_10710, D1G09_02880, D1G21_09940, EF900_08555, EP54_02130, EQ90_01235, ERS072840_ ...Gene: rplX, rplX_1, BN1321_380071, BTN44_13615, C7P97_04090, CSC83_04375, CSC87_02265, CV021_17970, D1G01_09470, D1G05_10710, D1G09_02880, D1G21_09940, EF900_08555, EP54_02130, EQ90_01235, ERS072840_01145, HMPREF3211_01564, M1K003_0782, NCTC10654_02411, NCTC10702_03537, NCTC13131_01582, NCTC5664_01673, NCTC6133_03029, NCTC7878_01020, NCTC7988_02318, RK64_11925, SAMEA1708674_02422
Production host: Staphylococcus aureus (bacteria) / References: UniProt: W8TRD5
#20: Protein 50S ribosomal protein L25 / General stress protein CTC


Mass: 10462.200 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria) / Gene: rplY, ctc, HMPREF3211_00359 / Production host: Staphylococcus aureus (bacteria) / References: UniProt: A0A133Q8Z9
#21: Protein 50S ribosomal protein L27


Mass: 8595.688 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rpmA, BN1321_260283, BTN44_03935, C7P97_01990, CSC87_06710, DD547_01728, DDL17_04730, DQV20_11650, DQV53_09150, E3A28_00245, E3K14_08310, E4U00_05985, EP54_09335, EQ90_02340, ERS072840_00777, ...Gene: rpmA, BN1321_260283, BTN44_03935, C7P97_01990, CSC87_06710, DD547_01728, DDL17_04730, DQV20_11650, DQV53_09150, E3A28_00245, E3K14_08310, E4U00_05985, EP54_09335, EQ90_02340, ERS072840_00777, ERS140147_00927, FA040_12875, FVP29_06755, G0V24_11260, G0X12_13450, G0X27_07875, G0Y02_04570, G0Z08_03090, G0Z18_03185, G6W67_00925, G6W97_12310, G6X24_04035, G6X31_01370, G6X35_09655, G6Y10_03070, G6Y24_01395, G6Y28_11450, G6Y30_16885, GF545_03605, GF559_00530, GIX97_04375, GO677_13480, GO706_06590, GO746_11040, GO788_00940, GO793_02920, GO793_12520, GO803_01990, GO805_05050, GO810_16080, GO821_00240, GO894_13400, GO915_03865, GO941_09810, GO942_04930, HMPREF3211_01797, M1K003_1396, NCTC10654_01709, NCTC10702_02584, NCTC5664_03623, NCTC6133_02187, NCTC9944_01669, RK64_08800, SAMEA1029528_01685, SAMEA1029547_02075, SAMEA1029553_02518, SAMEA1469856_00606, SAMEA1469884_01222, SAMEA1531680_00289, SAMEA1531701_01204, SAMEA1964876_02448, SAMEA1965205_02482, SAMEA1966505_02491, SAMEA1969349_02417, SAMEA1969845_01677, SAMEA1971706_01054, SAMEA1972827_01837, SAMEA2076212_01601, SAMEA2076218_01667, SAMEA2076220_01753, SAMEA2076226_01555, SAMEA2076463_02382, SAMEA2076464_02395, SAMEA2076470_02432, SAMEA2076472_02347, SAMEA2076478_02403, SAMEA2076480_02457, SAMEA2076481_02469, SAMEA2076743_02500, SAMEA2076745_02444, SAMEA2076746_02445, SAMEA2076747_02485, SAMEA2076749_02399, SAMEA2076751_02426, SAMEA2076752_02494, SAMEA2076755_02465, SAMEA2076756_02513, SAMEA2076758_02520, SAMEA2076759_02514, SAMEA2076761_02558, SAMEA2076762_01946, SAMEA2076763_01878, SAMEA2076764_02485, SAMEA2076765_02395, SAMEA2077023_02452, SAMEA2077025_02499, SAMEA2077027_02452, SAMEA2077029_02462, SAMEA2077031_02498, SAMEA2077034_01993, SAMEA2077035_02484, SAMEA2077039_02485, SAMEA2077040_02405, SAMEA2077041_02448, SAMEA2077044_02453, SAMEA2077045_02428, SAMEA2077046_02418, SAMEA2077293_02511, SAMEA2077294_02437, SAMEA2077295_02426, SAMEA2077297_02397, SAMEA2077300_02476, SAMEA2077301_02475, SAMEA2077302_02444, SAMEA2077303_02457, SAMEA2077307_02358, SAMEA2077832_02484, SAMEA2078252_02488, SAMEA2078256_02458, SAMEA2078307_02501, SAMEA2078308_02410, SAMEA2078553_02400, SAMEA2078558_02471, SAMEA2078560_02497, SAMEA2078569_00045, SAMEA2078570_02414, SAMEA2078572_02515, SAMEA2078824_02436, SAMEA2078837_02547, SAMEA2079048_02569, SAMEA2079051_02464, SAMEA2079277_01809, SAMEA2079291_02683, SAMEA2079503_02554, SAMEA2079507_01006, SAMEA2079512_02668, SAMEA2079517_02675, SAMEA2079724_01338, SAMEA2079727_01613, SAMEA2079728_01942, SAMEA2079732_01657, SAMEA2079742_01685, SAMEA2079946_01625, SAMEA2079949_01592, SAMEA2079951_01554, SAMEA2079952_01420, SAMEA2079957_01911, SAMEA2079958_01821, SAMEA2079960_01836, SAMEA2079961_01610, SAMEA2079968_01341, SAMEA2080329_01469, SAMEA2080330_01911, SAMEA2080334_01931, SAMEA2080433_01741, SAMEA2080812_02523, SAMEA2080898_02517, SAMEA2080900_02444, SAMEA2080904_02458, SAMEA2080913_02506, SAMEA2081043_02491, SAMEA2081053_02568, SAMEA2081054_02532, SAMEA2081055_02520, SAMEA2081060_01822, SAMEA2081211_00856, SAMEA2081213_00880, SAMEA2081218_01648, SAMEA2081341_01342, SAMEA2081342_01613, SAMEA2081349_01863, SAMEA2081359_01618, SAMEA2081362_01624, SAMEA2081468_02420, SAMEA2081474_02497, SAMEA2081475_02468, SAMEA2081476_02434, SAMEA2081479_02516, SAMEA2081480_02465, SAMEA2081560_02362, SAMEA2081561_02438, SAMEA2081564_02417, SAMEA2081567_02405, SAMEA2081568_02501, SAMEA2081569_02477, SAMEA2081570_02368, SAMEA2081571_02440, SAMEA2081572_02440, SAMEA2081573_02445, SAMEA2081575_02416, SAMEA2081577_02471, SAMEA2081578_02439, SAMEA2081579_02465, SAMEA2081581_02377, SAMEA2081582_02552, SAMEA2081673_01457, SAMEA2081674_01694, SAMEA2445518_00238, SAMEA958766_01142, SAMEA958770_01542, SAMEA958772_01368, SAMEA958778_01376, SAMEA958779_02721, SAMEA958785_01191, SAMEA958793_01566, SAMEA958804_01430, SAMEA958810_02900, SAMEA958836_01613, SAMEA958838_02813, SAMEA958845_01499, SAMEA958846_01745, SAMEA958848_02334, SAMEA958855_02127, SAMEA958858_02292, SAMEA958870_02488, SAMEA958898_01784, SAMEA958906_02169, SAMEA958924_01278, SAMEA958925_01300, SAMEA958951_01297, SAMEA958953_00897, SAMEA958961_01997, SAMEA958979_01147, SAMEA958987_02152, SAMEA958995_01154, SAST44_01825, SAST45_01803
Production host: Staphylococcus aureus (bacteria) / References: UniProt: A0A077ULC5
#22: Protein/peptide 50S ribosomal protein L28


Mass: 5499.432 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rpmB, rpmB_1, BN1321_240068, BTN44_06590, C7P97_10230, CSC83_01035, CSC87_04080, CV021_11420, D1G01_05860, D1G05_09135, D1G09_05585, D1G21_01100, EF900_02800, EP54_08520, EQ90_08140, ERS072840_ ...Gene: rpmB, rpmB_1, BN1321_240068, BTN44_06590, C7P97_10230, CSC83_01035, CSC87_04080, CV021_11420, D1G01_05860, D1G05_09135, D1G09_05585, D1G21_01100, EF900_02800, EP54_08520, EQ90_08140, ERS072840_01399, ERS140147_00162, HMPREF3211_01267, M1K003_0065, NCTC10654_01268, NCTC10702_01929, NCTC13131_00418, NCTC5664_02292, NCTC6133_01484, NCTC7878_01902, NCTC7988_01236, RK64_06525, SAMEA1708664_00224, SAMEA1708674_00029, SAMEA2445518_00654
Production host: Staphylococcus aureus (bacteria) / References: UniProt: A0A077URJ8
#23: Protein 50S ribosomal protein L29


Mass: 7845.942 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rpmC, rpmC_1, BN1321_380074, BTN44_13630, C7P97_04105, CSC83_04390, CSC87_02250, CSC87_16445, CV021_17955, D1G01_09455, D1G05_10695, D1G09_02865, D1G21_09925, EF900_08540, EP54_02115, EQ90_ ...Gene: rpmC, rpmC_1, BN1321_380074, BTN44_13630, C7P97_04105, CSC83_04390, CSC87_02250, CSC87_16445, CV021_17955, D1G01_09455, D1G05_10695, D1G09_02865, D1G21_09925, EF900_08540, EP54_02115, EQ90_01250, ERS072840_01148, ERS140147_00630, HMPREF3211_01567, M1K003_0785, NCTC10654_02414, NCTC10702_03540, NCTC13131_01585, NCTC5664_01670, NCTC6133_03032, NCTC7878_01017, NCTC7988_02321, RK64_11940, SAMEA1708664_00134, SAMEA1708674_02419, SAMEA2445518_02260
Production host: Staphylococcus aureus (bacteria) / References: UniProt: A0A077W1J5
#24: Protein 50S ribosomal protein L30


Mass: 6434.487 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria)
Gene: rpmD, rpmD_1, BN1321_380064, BTN44_13580, C7P97_04055, CSC83_04340, CSC87_02300, CV021_18005, D1G01_09505, D1G05_10745, D1G09_02915, D1G21_09975, EF900_08590, EP54_02165, EQ90_01200, ERS072840_ ...Gene: rpmD, rpmD_1, BN1321_380064, BTN44_13580, C7P97_04055, CSC83_04340, CSC87_02300, CV021_18005, D1G01_09505, D1G05_10745, D1G09_02915, D1G21_09975, EF900_08590, EP54_02165, EQ90_01200, ERS072840_01138, HMPREF3211_01557, M1K003_0775, NCTC10654_02404, NCTC10702_03528, NCTC13131_01575, NCTC5664_01681, NCTC6133_03022, NCTC7878_01028, NCTC7988_02311, RK64_11890, SAMEA1708664_01776, SAMEA1708674_02429
Production host: Staphylococcus aureus (bacteria) / References: UniProt: W8UVN7
#25: Protein/peptide 50S ribosomal protein L32


Mass: 5569.565 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Staphylococcus aureus (bacteria) / Gene: rpmF, BSZ10_12860 / Production host: Staphylococcus aureus (bacteria) / References: UniProt: A0A1Q8DAT0

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: 50S ribosomal subunit / Type: RIBOSOME / Entity ID: all / Source: NATURAL
Molecular weightExperimental value: NO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Buffer solutionpH: 7.6
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: COPPER / Grid mesh size: 200 divisions/in. / Grid type: Quantifoil R1.2/1.3
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD
Image recordingElectron dose: 47 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k)

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Processing

SoftwareName: PHENIX / Version: 1.18_3845: / Classification: refinement
CTF correctionType: NONE
3D reconstructionResolution: 2.73 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 175844 / Symmetry type: POINT
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.00788253
ELECTRON MICROSCOPYf_angle_d1.02133220
ELECTRON MICROSCOPYf_dihedral_angle_d20.65736884
ELECTRON MICROSCOPYf_chiral_restr0.05517236
ELECTRON MICROSCOPYf_plane_restr0.0076260

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