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- EMDB-11902: Staphylococcus aureus 70S after 30 minutes incubation at 37C -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-11902
TitleStaphylococcus aureus 70S after 30 minutes incubation at 37C
Map data
Sample
  • Complex: 70S
    • RNA: x 3 types
    • Protein or peptide: x 44 types
KeywordsRibosome / H68 / translation / protein synthesis
Function / homology
Function and homology information


large ribosomal subunit / small ribosomal subunit / ribosomal large subunit assembly / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding ...large ribosomal subunit / small ribosomal subunit / ribosomal large subunit assembly / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / zinc ion binding / cytoplasm
Similarity search - Function
Ribosomal protein L31 type B / Type-1 KH domain profile. / : / Ribosomal protein S14, type Z / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L16 signature 1. / : ...Ribosomal protein L31 type B / Type-1 KH domain profile. / : / Ribosomal protein S14, type Z / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / : / : / Ribosomal protein L28 / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein S14/S29 / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein L20 signature. / Ribosomal protein S6 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S4, bacterial-type / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein S6, plastid/chloroplast / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L30, bacterial-type / Ribosomal protein L33 superfamily / Ribosomal protein L16 / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / L28p-like / Ribosomal protein L20 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L21 / Ribosomal protein S15, bacterial-type / Ribosomal proteins 50S L24/mitochondrial 39S L24 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein S6 / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein S12, bacterial-type / Ribosomal protein L24 / Ribosomal protein L34 / Ribosomal protein L34 / Ribosomal protein L13, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein L3, bacterial/organelle-type / Ribosomal protein L15, bacterial-type / Ribosomal protein S18 / Ribosomal protein S18 / 50S ribosomal protein uL4 / Ribosomal protein S18 superfamily / K Homology domain / K homology RNA-binding domain / Ribosomal protein S3, conserved site / Ribosomal protein S3 signature. / Ribosomal protein S14, conserved site / Ribosomal protein S14 signature. / Ribosomal protein L5, conserved site / Ribosomal protein L5 signature. / KH domain / Type-2 KH domain profile. / K Homology domain, type 2 / Ribosomal protein S3, C-terminal
Similarity search - Domain/homology
Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL14 / Small ribosomal subunit protein bS18 ...Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL14 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS9 / : / : / : / : / : / : / : / Large ribosomal subunit protein bL21 / Small ribosomal subunit protein bS6 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein bL31B / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein bS20
Similarity search - Component
Biological speciesStaphylococcus aureus (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.11 Å
AuthorsCimicata G / Bashan A / Yonath A
Funding support1 items
OrganizationGrant numberCountry
European Research Council (ERC)322581
CitationJournal: To Be Published
Title: Staphylococcus aureus 70S after 30 minutes incubation a 37C
Authors: Cimicata G / Bashan A / Yonath A
History
DepositionOct 27, 2020-
Header (metadata) releaseNov 17, 2021-
Map releaseNov 17, 2021-
UpdateOct 23, 2024-
Current statusOct 23, 2024Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0052
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.0052
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-7aso
  • Surface level: 0.0052
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_11902.png

Downloads & links

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Map

FileDownload / File: emd_11902.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.86 Å/pix.
x 420 pix.
= 361.2 Å		0.86 Å/pix.
x 420 pix.
= 361.2 Å		0.86 Å/pix.
x 420 pix.
= 361.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.86 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0052 / Movie #1: 0.0052
Minimum - Maximum-0.015758734 - 0.03910529
Average (Standard dev.)0.0002529819 (±0.0021330114)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions420420420
Spacing420420420
CellA=B=C: 361.2 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.860.860.86
M x/y/z420420420
origin x/y/z0.0000.0000.000
length x/y/z361.200361.200361.200
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS420420420
D min/max/mean-0.0160.0390.000

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Supplemental data

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Additional map: #1

Fileemd_11902_additional_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : 70S

EntireName: 70S
Components
  • Complex: 70S
    • RNA: 5S
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14 type Z
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
    • RNA: 16S
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L25
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomalprotein L32p
    • Protein or peptide: 50S ribosomal protein L33
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • Protein or peptide: 50S ribosomal protein L31 type B
    • RNA: 23S

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Supramolecule #1: 70S

SupramoleculeName: 70S / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#47
Source (natural)Organism: Staphylococcus aureus (bacteria)

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Macromolecule #1: 5S

MacromoleculeName: 5S / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 36.692801 KDa
SequenceString:
UCUGGUGACU AUAGCAAGGA GGUCACACCU GUUCCCAUGC CGAACACAGA AGUUAAGGUC UUUAGCGACG AUGGUAGCCA ACUUACGUU CCGCUAGAGU AGAACGUUGC CAGGC

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Macromolecule #20: 16S

MacromoleculeName: 16S / type: rna / ID: 20
Details: ...Details: UUUAUGGAGAGUUUGAUCCUGGCUCAGGAUGAACGCUGGCGGCGUGCCUAAUACAUGCAAGUCGAGCGAACGGACGAGAAGCUUGCUUCUCUGAUGUUAGCGGCGGACGGGUGAGUAACACGUGGAUAACCUACCUAUAAGACUGGGAUAACUUCGGGAAACCGGAGCUAAUACCGGAUAAUAUUUUGAACCGCAUGGUUCAAAAGUGAAAGACGGUCUUGCUGUCACUUAUAGAUGGAUCCGCGCUGCAUUAGCUAGUUGGUAAGGUAACGGCUUACCAAGGCAACGAUACGUAGCCGACCUGAGAGGGUGAUCGGCCACACUGGAACUGAGACACGGUCCAGACUCCUACGGGAGGCAGCAGUAGGGAAUCUUCCGCAAUGGGCGAAAGCCUGACGGAGCAACGCCGCGUGAGUGAUGAAGGUCUUCGGAUCGUAAAACUCUGUUAUUAGGGAAGAACAUAUGUGUAAGUAACUGUGCACAUCUUGACGGUACCUAAUCAGAAAGCCACGGCUAACUACGUGCCAGCAGCCGCGGUAAUACGUAGGUGGCAAGCGUUAUCCGGAAUUAUUGGGCGUAAAGCGCGCGUAGGCGGUUUUUUAAGUCUGAUGUGAAAGCCCACGGCUCAACCGUGGAGGGUCAUUGGAAACUGGAAAACUUGAGUGCAGAAGAGGAAAGUGGAAUUCCAUGUGUAGCGGUGAAAUGCGCAGAGAUAUGGAGGAACACCAGUGGCGAAGGCGACUUUCUGGUCUGUAACUGACGCUGAUGUGCGAAAGCGUGGGGAUCAAACAGGAUUAGAUACCCUGGUAGUCCACGCCGUAAACGAUGAGUGCUAAGUGUUAGGGGGUUUCCGCCCCUUAGUGCUGCAGCUAACGCAUUAAGCACUCCGCCUGGGGAGUACGACCGCAAGGUUGAAACUCAAAGGAAUUGACGGGGACCCGCACAAGCGGUGGAGCAUGUGGUUUAAUUCGAAGCAACGCGAAGAACCUUACCAAAUCUUGACAUCCUUUGACAACUCUAGAGAUAGAGCCUUCCCCUUCGGGGGACAAAGUGACAGGUGGUGCAUGGUUGUCGUCAGCUCGUGUCGUGAGAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUAAGCUUAGUUGCCAUCAUUAAGUUGGGCACUCUAAGUUGACUGCCGGUGACAAACCGGAGGAAGGUGGGGAUGACGUCAAAUCAUCAUGCCCCUUAUGAUUUGGGCUACACACGUGCUACAAUGGACAAUACAAAGGGCAGCGAAACCGCGAGGUCAAGCAAAUCCCAUAAAGUUGUUCUCAGUUCGGAUUGUAGUCUGCAACUCGACUACAUGAAGCUGGAAUCGCUAGUAAUCGUAGAUCAGCAUGCUACGGUGAAUACGUUCCCGGGUAUUGUACACACCGCCCGUCACACCACGAGAGUUUGUAACACCCGAAGCCGGUGGAGUAACCUUUUAGGAGCUAGCCGUCGAAGGUGGGACAAAUGAUUGGGGUGAAGUCGUAACAAGGUAGCCGUAUCGGAAGGUGCGGCUGGAUCACCUCCUUU
Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 458.188688 KDa
SequenceString: AUGGAGAGUU UGAUCCUGGC UCAGGAUGAA CGCUGGCGGC GUGCCUAAUA CAUGCAAGUC GAGCGAACGU UAGCGGCGGA CGGGUGAGU AACACGUGGA UAACCUACCU AUAAGACUGG GAUAACUUCG GGAAACCGUA GCUAAUACCG GAUAAUAUAG U GAAAGACA ...String:
AUGGAGAGUU UGAUCCUGGC UCAGGAUGAA CGCUGGCGGC GUGCCUAAUA CAUGCAAGUC GAGCGAACGU UAGCGGCGGA CGGGUGAGU AACACGUGGA UAACCUACCU AUAAGACUGG GAUAACUUCG GGAAACCGUA GCUAAUACCG GAUAAUAUAG U GAAAGACA CUUAUAGAUG GAUCCGCGCU GCAUUAGCUA GUUGGUAAGG UAACGGCUUA CCAAGGCAAC GAUGCAUAGC CG ACCUGAG AGGGUGAUCG GCCACACUGG AACUGAGACA CGGUCCAGAC UCCUACGGGA GGCAGCAGUA GGGAAUCUUC CGC AAUGGG CGAAAGCCUG ACGGAGCAAC GCCGCGUGAG UGAUGAAGGU CUAUCGUAAA ACUCUGUUAU UAGGGAGAAC AUAU GUCAC AUCUUGACGU ACCUAAUCAG AAAGCCACGG CUAACUACGU GCCAGCAGCC GCGGUAAUAC GUAGGUGGCA AGCGU UAUC CGGAAUUAUU GGGCGUAAAG CGCGCGUAGG CGGUUUUUUA AGUCUGAUGU GAAAGCCCAC GGCUCAACCG UGGAGG GUC AUUGGAAACU GGAAAACUUG AGUGCAGAAG AGGAAAGUGG AAUUCCAUGU GUAGCGGUGA AAUGCGCGAG AUAUGGA GG AACACCAGUG GCGAAGGCGA CUUUCUGGUC UGUAACUGAC GCUGAUGUGC GAAAGCGUGG GGAUCAAACA GGAUUAGA U ACCCUGGUAG UCCACGCCGU AAACGAUGAG UGCUAAGUGU UAGGGGGUGC CCCUUAGUGC UGCAGCUAAC GCAUUAAGC ACUCCGCCUG GGGAGUACGA CCGCAAGGUU GAAACUCAAA GGAAUUGACG GGGACCCGCA CAAGCGGUGG AGCAUGUGGU UUAAUUCGA AGCAACGCGA AGAACCUUAC CAAAUCUUGA CAUCACAAAG UGACAGGUGG UGCAUGGUUG UCGUCAGCUC G UGUCGUGA GAUGUUGGGU UAAGUCCCGC AACGAGCGCA ACCCUUAAGC UUAGUUGCCA UCAUUAAGUU GGGCACUCUA AG UUGACUG CCGGUGACAA ACCGGAGGAA GGUGGGGAUG ACGUCAAAUC AUCAUGCCCC UUAUGAUUUG GGCUACACAC GUG CUACAA UGGACAAUAC AAAGGGCAGC GAAACCGCGA GGUCAAGCAA UCCCAUAAAG UUGUUCUCAG UUCGGAUUGU AGUC UGCAA CUCGACUACA UGAAGCUGGA AUCGCUAGUA AUCGUAGAUC AGCAUGCUAC GGUGAAUACG UUCCCGGGUC UUGUA CACA CCGCCCGUCA CACCACGAGG UUUGUAACAC CCGAAGCCGG UGGAGUAACC UUUUAGGAGC UAGCCGUCGA AGGUGG GAC AAAUGGGGGU GAAGUCGUAA CAAGGUAGCC GUAUCGGAAG GUGCGGCUGG AU

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Macromolecule #47: 23S

MacromoleculeName: 23S / type: rna / ID: 47
Details: GAUUAAGUUAUUAAGGGCGCACGGUGGAUGCCUUGGCACUAGAAGCCGAUGAAGGACGUUACUAACGACGAUAUGCUUUGGGGAGCUGUAAGCUUUGAUCCAGAGAUUUCCGAAUGGGGAAACCCAGCAUGAGUUA ...Details: GAUUAAGUUAUUAAGGGCGCACGGUGGAUGCCUUGGCACUAGAAGCCGAUGAAGGACGUUACUAACGACGAUAUGCUUUGGGGAGCUGUAAGCUUUGAUCCAGAGAUUUCCGAAUGGGGAAACCCAGCAUGAGUUA UGUCAUGUUAUCGAUAUGUGAAUACAUAGCAUAUCAGAAGGCACACCCGGAGAACUGAAACAUCUUAGUA CCCGGAGGAAGAGAAAGAAAAUUCGAUUCCCUUAGUAGCGGCGAGCGAAACGGGAAGAGCCCAAACCAAC AAGCUUGCUUGUUGGGGUUGUAGGACACUCUGUACGGAGUUACAAAGGACGACAUUAGACGAAUCAUCUG GAAAGAUGAAUCAAAGAAGGUAAUAAUCCUGUAGUCGAAAAUGUUGUCUCUCUUGAGUGGAUCCUGAGUA CGACGGAGCACGUGAAAUUCCGUCGGAAUCUGGGAGGACCAUCUCCUAAGGCUAAAUACUCUCUAGUGAC CGAUAGUGAACCAGUACCGUGAGGGAAAGGUGAAAAGCACCCCGGAAGGGGAGUGAAAUAGAACCUGAAA CCGUGUGCUUACAAGUAGUCAGAGCCCGUUAAUGGGUGAUGGCGUGCCUUUUGUAGAAUGAACCGGCGAG UUACGAUUUGAUGCAAGGUUAAGCAGUAAAUGUGGAGCCGUAGCGAAAGCGAGUCUGAAUAGGGCGUUUA GUAUUUGGUCGUAGACCCGAAACCAGGUGAUCUACCCUUGGUCAGGUUGAAGUUCAGGUAACACUGAAUG GAGGACCGAACCGACUUACGUUGAAAAGUGAGCGGAUGAACUGAGGGUAGCGGAGAAAUUCCAAUCGAAC CUGGAGAUAGCUGGUUCUCUCCGAAAUAGCUUUAGGGCUAGCCUCAAGUGAUGAUUAUUGGAGGUAGAGC ACUGUUUGGACGAGGGGCCCCUCUCGGGUUACCGAAUUCAGACAAACUCCGAAUGCCAAUUAAUUUAACU UGGGAGUCAGAACAUGGGUGAUAAGGUCCGUGUUCGAAAGGGAAACAGCCCAGACCACCAGCUAAGGUCC CAAAAUAUAUGUUAAGUGGAAAAGGAUGUGGCGUUGCCCAGACAACUAGGAUGUUGGCUUAGAAGCAGCC AUCAUUUAAAGAGUGCGUAAUAGCUCACUAGUCGAGUGACACUGCGCCGAAAAUGUACCGGGGCUAAACA UAUUACCGAAGCUGUGGAUUGUCCUUUGGACAAUGGUAGGAGAGCGUUCUAAGGGCGUUGAAGCAUGAUC GUAAGGACAUGUGGAGCGCUUAGAAGUGAGAAUGCCGGUGUGAGUAGCGAAAGACGGGUGAGAAUCCCGU CCACCGAUUGACUAAGGUUUCCAGAGGAAGGCUCGUCCGCUCUGGGUUAGUCGGGUCCUAAGCUGAGGCC GACAGGCGUAGGCGAUGGAUAACAGGUUGAUAUUCCUGUACCACCUAUAAUCGUUUUAAUCGAUGGGGGG ACGCAGUAGGAUAGGCGAAGCGUGCGAUUGGAUUGCACGUCUAAGCAGUAAGGCUGAGUAUUAGGCAAAU CCGGUACUCGUUAAGGCUGAGCUGUGAUGGGGAGAAGACAUUGAGUCUUCGAGUCGUUGAUUUCACACUG CCGAGAAAAGCCUCUAGAUAGAAAAUAGGUGCCCGUACCGCAAACCGACACAGGUAGUCAAGAUGAGAAU UCUAAGGUGAGCGAGCGAACUCUCGUUAAGGAACUCGGCAAAAUGACCCCGGUAACUUCGGGAGAAGGGGU GCUCUUUAGGGUUAACGCCCAGAAGAGCCGCAGUGAAUAGGCCCAAGCGACUGUUUAUCAAAAACACAGG UCUCUGCUAAACCGUAAGGUGAUGUAUAGGGGCUGACGCCUGCCCGGUGCUGGAAGGUUAAGAGGAGUGG UUAGCUUCUGCGAAGCUACGAAUCGAAGCCCCAGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAG CGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGAAAGGCGUAACGAUUUGGGCACUGUCUCAACGAGA GACUCGGUGAAAUCAUAGUACCUGUGAAGAUGCAGGUUACCCGCGACAGGACGGAAAGACCCCGUGGAGC UUUACUGUAGCCUGAUAUUGAAAUUCGGCACAGCUUGUACAGGAUAGGUAGGAGCCUUUGAAACGUGAGC GCUAGCUUACGUGGAGGCGCUGGUGGGAUACUACCCUAGCUGUGUUGGCUUUCUAACCCGCACCACUUAU CGUGGUGGGAGACAGUGUCAAGCGGGCAGUUUGACUGGGGCGGUCGCCUCCUAAAAGGUAACGGAGGCGC UCAAAGGUUCCCUCAGAAUGGUUGGAAAUCAUUCAUAGAGUGUAAAGGCAUAAGGGAGCUUGACUGCGAG ACCUACAAGUCGAGCAGGGUCGAAAGACGGACUUAGUGAUCCGGUGGUUCCGCAUGGAAGGGCCAUCGCU CAACGGAUAAAAGCUACCCCGGGGAUAACAGGCUUAUCUCCCCCAAGAGUUCACAUCGACGGGGAGGUUU GGCACCUCGAUGUCGGCUCAUCGCAUCCUGGGGCUGUAGUCGGUCCCAAGGGUUGGGCUGUUCGCCCAUU AAAGCGGUACGCGAGCUGGGUUCAGAACGUCGUGAGACAGUUCGGUCCCUAUCCGUCGUGGGCGUAGGAA AUUUGAGAGGAGCUGUCCUUAGUACGAGAGGACCGGGAUGGACAUACCUCUGGUGUACCAGUUGUCGUGC CAACGGCAUAGCUGGGUAGCUAUGUGUGGACGGGAUAAGUGCUGAAAGCAUCUAAGCAUGAAGCCCCCCU CAAGAUGAGAUUUCCCAACUUCGGUUAUAAGAUCCCUCAAAGAUGAUGAGGUUAAUAGGUUCGAGGUGGA AGCAUGGUGACAUGUGGAGCUGACGAAUACUAAUCGAUCGAAGACUUAAUCAA
Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 881.281938 KDa
SequenceString: UUAAGUUAUU AAGGGCGCAC GGUGGAUGCC UUGGCACUAG AAGCCAAUGA AGGACGUUAC UAACGACGAU AUGCUUUGGG GAGCUGUAA GUAAGCUUUG AUCCAGAGAU UUCCGAAUGG GGAAACCCAG CAUGAGUUAU GUCAUGUUAU CGAUAUGUGA A UACAUAGC ...String:
UUAAGUUAUU AAGGGCGCAC GGUGGAUGCC UUGGCACUAG AAGCCAAUGA AGGACGUUAC UAACGACGAU AUGCUUUGGG GAGCUGUAA GUAAGCUUUG AUCCAGAGAU UUCCGAAUGG GGAAACCCAG CAUGAGUUAU GUCAUGUUAU CGAUAUGUGA A UACAUAGC AUAUCAGAAG GCACACCCGG AGAACUGAAA CAUCUUAGUA CCCGGAGGAA GAGAAAGAAA AUUCGAUUCC CU UAGUAGC GGCGAGCGAA ACGGGAAGAG CCCAAACCAA CAAGCUUGCU UGUUGGGGUU GUAGGACACU CUAGAGUUAC AAA GGACGA CAUUAGACGA AUCAUCUGGA AAGAUGAAUC AAAGAAGGUA AUAAUCCUGU AGUCGAAAAU GUUGUCUCUC UUGA GUGGA UCCUGAGUAC GACGGAGCAC GUGAAAUUCC GUCGGAAUCU GGGAGGACCA UCUCCUAAGG CUAAAUACUC UCUAG UGAC CGAUAGUGAA CCAGUACCGU GAGGGAAAGG UGAAAAGCAC CCCGGAAGGG GAGUGAAAUA GAACCUGAAA CCGUGU GCU UACAAGUAGU CAGAGCCCGU UAAUGGGUGA UGGCGUGCCU UUUGUAGAAU GAACCGGCGA GUUACGAUUU GAUGCAA GG UUAAGCAGUA AAUGUGGAGC CGUAGCGAAA GCGAGUCUGA AUAGGGCGUU UAGUAUUUGG UCGUAGACCC GAAACCAG G UGAUCUACCC UUGGUCAGGU UGAAGUUCAG UGAAUGGAGG ACCGAACCGA CUUACGUUGA AAAGUGAGCG GAUGAACUG AGGGUAGCGG AGAAAUUCCA AUCGAACCUG GAGAUAGCUG GUUCUCUCCG AAAUAGCUUU AGGGCUAGCC UCAAGUGAUG AUUAUUGGA GGUAGAGCAC UGUUUGGACG AGGCCGAAUU CAGACAAACU CCGAAUGCCA AUUAAUUUAA CUUGGGAGUC A GAACAUGG GUGAUAAGGU CCGUGUUCGA AAGGGAAACA GCCCAGACCA CCAGCUAAGG UCCCAAAAUA UAUGUUAAGU GG AAAAGGA UGUGGCGUUG CCACAACUAG AGUCGAGUGA CACUGCGCCG AAAAUGUACC GGGGCUAAAC AUAUUACCGA AGC UGUGGA UUGUCCUUGG ACAAUGGUAG GAGAGCGUUC UAAGGGCGUU GAAGCAUGAU CGUAAGGACA UGUGGAGCGC UUAG AAGUG AGAAUGCCGG UGUGAGUAGC GAAAGACGGG UGAGAAUCCC GUCCACCGAU UGACUAAGGU UUCCAGAGGA AGGCU CGUC CGCUCUGGGU UAGUCGGGUC CUAAGCUGAG GCCGACAGGC GUAGGCGAUG GAUAACAGGU UGAUAUUCCU GUACCA CCU AUAAUCGAUG GGGGGACGCA GUAGGAUAGG GAAGCGUUGG AUUGCACGUC UAAGCAGUAA GGCUGAGUAA UCCGGUA CU CGGGCUGAGC UGUGAUGGGG AGAAGACCGA GUCGUUGAUU UCACACUGCC GAGAAAAGCC UCUAGAUAGA AAAUAGGU G CCCGUACCGC AAACCGACAC AGGUAGUCAA GAUGAGAAUU CUAAGGUGAG CGAGCGAACU CUCGUUAAGG AACUCGGCA AAAUGACCCC GUAACUUCGG GAGAAGGGGU GCUCUUUAGG GUUAACGCCC AGAAGAGCCG CAGUGAAUAG GCCCAAGCGA CUGUUUAUC AAAAACACAG GUCUCUGCUA AACCGUAAGG UGAUGUAUAG GGGCUGACGC CUGCCCGGUG CUGGAAGGUU A AGAGGAGU GGUUAGCUUC UGCGAAGCUA CGAAUCGAAG CCCCAGUAAA CGGCGGCCGU AACGGUCCUA AGGUAGCGAA AU UCCUUGU CGGGUAAGUU CCGACCCGCA CGAAAGGCGU AACGAUUUGG GCACUGUCUC AACGAGAGAC UCGGUGAAAU CAU AGUACC UGUGAAGAUG CAGGUUACCC GCGACAGGAC GGAAAGACCC CGUGGAGCUU UACUGUAGCC UGAUAUUGAA AUUC GGCAC AGCUAGCUGU GUUGGCUUUC UAACCCGCAC CACUUACGUG GUGGGAGACA GUGUCAAGCG GGCAGUUUGA CUGGG GCGG UCGCCUCCUA AAAGGUAACG GAGGCGCUCA AAGGUUCCCU CAGAAUGGUU GGAAAUCAUU CAUAGAGUGU AAAGGC AUA AGGGAGCUUG ACUGCGAGAC CUACAAGUCG AGCAGGGUCG AAAGACGGAC UUAGUGAUCC GGUGGUUCCG CAUGGAA GG GCCAUCGCUC AACGGAUAAA AGCUACCCCG GGGAUAACAG GCUUAUCUCC CCCAAGAGUU CACAUCGACG GGGAGGUU U GGCACCUCGA UGUCGGCUCA UCGCAUCCUG GGGCUGUAGU CGGUCCCAAG GGUUGGGCUG UUCGCCCAUU AAAGCGGUA CGCGAGCUGG GUUCAGAACG UCGUGAGACA GUUCGGUCCC UAUCCGUCGU GGGCGUAGGA AAUUUGAGAG GAGCUGUCCU UAGUACGAG AGGACCGGGA UGGACAUACC UCUGGUGUAC CAGUUGUCGU GCCAACGGCA UAGCUGGGUA GCUAUGUGUG G ACGGGAUA AGUGCUGAAA GCAUCUAAGC AUGAAGCCCC CCUCAAGAUG AGAUUUCCCA ACUCGGUUAU AAGAUCCCUC AA AGAUGAU GAGGUUAAUA GGUUCGAGGU GGAAGCAUGG UGACAUGUGG AGCUGACGAA UACUAAUCGA UCGAAGACUU AAU

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Macromolecule #2: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 22.684201 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString: GQKINPIGLR VGIIRDWEAK WYAEKDFASL LHEDLKIRKF IDNELKEASV SHVEIERAAN RINIAIHTGK PGMVIGKGGS EIEKLRNKL NALTDKKVHI NVIEIKKVDL DARLVAENIA RQLENRASFR RVQKQAITRA MKLGAKGIKT QVSGRLGGAD I ARAEQYSE ...String:
GQKINPIGLR VGIIRDWEAK WYAEKDFASL LHEDLKIRKF IDNELKEASV SHVEIERAAN RINIAIHTGK PGMVIGKGGS EIEKLRNKL NALTDKKVHI NVIEIKKVDL DARLVAENIA RQLENRASFR RVQKQAITRA MKLGAKGIKT QVSGRLGGAD I ARAEQYSE GTVPLHTLRA DIDYAHAEAD TTYGKLGVKV WIYR

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #3: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 22.849145 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString: RFRGSNWKKS RRLGISLSGT GKELEKRPYA PGQHGPNQRK KLSEYGLQLR EKQKLRYLYG MTERQFRNTF DIAGKKFGVH GENFMILLA SRLDAVVYSL GLARTRRQAR QLVNHGHILV DGKRVDIPSY SVKPGQTISV REKSQKLNII VESVEINNFV P EYLNFDAD ...String:
RFRGSNWKKS RRLGISLSGT GKELEKRPYA PGQHGPNQRK KLSEYGLQLR EKQKLRYLYG MTERQFRNTF DIAGKKFGVH GENFMILLA SRLDAVVYSL GLARTRRQAR QLVNHGHILV DGKRVDIPSY SVKPGQTISV REKSQKLNII VESVEINNFV P EYLNFDAD SLTGTFVRLP ERSELPAEIN EQLIVEYYSR

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #4: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 16.522119 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
EFEERVVTIN RVAKVVKGGR RFRFTALVVV GDKNGRVGFG TGKAQEVPEA IKKAVEAAKK DLVVVPRVEG TTPHTITGRY GSGSVFMKP AAPGTGVIAG GPVRAVLELA GITDILSKSL GSNTPINMVR ATIDGLQNLK NAEDVAKLRG KTVEELY

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #5: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 11.239766 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
MRTYEVMYIV RPNIEEDAKK ALVERFNGIL ATEGAEVLEA KDWGKRRLAY EINDFKDGFY NIVRVKSDNN KATDEFQRLA KISDDIIRY MVIRED

UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #6: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 17.695357 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
PRKGSVPKRD VLPDPIHNSK LVTKLINKIM LDGKRGTAQR ILYSAFDLVE QRSGRDALEV FEEAINNIMP VLEVKARRVG GSNYQVPVE VRPERRTTLG LRWLVNYARL RGEKTMEDRL ANEILDAANN TGGAVKKRED THKMAEANKA FAHYRW

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #7: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 14.622014 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
MTDPIADMLT RVRNANMVRH EKLELPASNI KKEIAEILKS EGFIKNVEYV EDDKQGVLRL FLKYGQNDER VITGLKRISK PGLRVYAKA SEMPKVLNGL GIALVSTSEG VITDKEARKR NVGGEIIAYV W

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #8: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 14.312321 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
VEYRGTGRRK NSVARVRLVP GEGNITVNNR DVREYLPFES LILDLNQPFD VTETKGNYDV LVNVHGGGFT GQAQAIRHGI ARALLEADP EYRGSLKRAG LLTRDPRMKE RKKPGLKAAR RSPQFSKR

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #9: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 9.24361 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
IRIRLKAYDH RVIDQSAEKI VETAKRSGAD VSGPIPLPTE KSVYTIIRAV HKYKDSREQF EQRTHKRLID IVNPTPKTVD

UniProtKB: UNIPROTKB: A0A6H0HKY4

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Macromolecule #10: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 12.047609 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
NIENGVAHIR STFNNTIVTI TDEFGNALSW SSAGALGFKG SKKSTPFAAQ MASETASKSA MEHGLKTVEV TVKGPGPGRE SAIRALQSA GLEVTAIRDV TPVPHNGCRP PKRRR

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #11: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 15.189673 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
PTINQLVRKP RQSKIKKSDS PALNKGFNSK KKKFTDLNSP QKRGVCTRVG TMTPKKPNSA LRKYARVRLS NNIEINAYIP GIGHNLQEH SVVLVRGGRV KDLPGVRYHI VRGALDTSGV DGRRQGRSLY GTKKPKN

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #12: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 12.843768 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
ARIAGVDIPR EKRVVISLTY IYGIGTSTAQ KILEEANVSA DTRVKDLTDD ELGRIREVVD GYKVEGDLRR ETNLNIKRLM EISSYRGIR HRRGLPVRGQ KTKNNARTRK GPVK

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #13: 30S ribosomal protein S14 type Z

MacromoleculeName: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 7.186573 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
AKTSMVAKQQ KKQKYAVREY TRCERCGRPH SVYRKFKLCR ICFRELAYKG QIPGVRKASW

UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #14: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.503135 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
AISQERKNEI IKEYRVHETD TGSPEVQIAV LTAEINAVNE HLRTHKKDHH SRRGLLKMVG RRRHLLNYLR SKDIQRYREL IKSLGIRR

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #15: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 9.291743 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
AVKIRLTRLG SKRNPFYRIV VADARSPRDG RIIEQIGTYN PTSANAPEIK VDEALALKWL NDGAKPTDTV HNILSKEGIM KKF

UniProtKB: UNIPROTKB: A0A6K3WEJ9

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Macromolecule #16: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 9.36686 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
NDRKVYVGKV VSDKMDKTIT VLVETYKTHK LYGKRVKYSK KYKTHDENNS AKLGDIVKIQ ETRPLSATKR FRLVEIVEES

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #17: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.574833 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
GITHIDYKDT ELLKRFISER GKILPRRVTG TSAKYQRMLT TAIKRSRHMA LLPYVK

UniProtKB: Small ribosomal subunit protein bS18

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Macromolecule #18: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 9.010406 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
SIKKGPFVDE HLMKKVEAQE GSEKKQVIKT WSRRSTIFPN FIGHTFAVYD GRKHVPVYVT EDMVGHKLGE FAPTRTFK

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #19: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 8.480758 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
KSAIKRVKTT EKAEARNISQ KSAMRTAVKN AKTAVSNNAD NKNELVSLAV KLVDKAAQSN LIHSNKADRI KSQLMTAN

UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #21: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 23.199545 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString: TKGILGRKIG MTQVFGENGE LIPVTVVEAK ENVVLQKKTV EVDGYNAIQV GFEDKKAYKK DAKSNKYANK PAEGHAKKAD AAPKRFIRE FRNVDVDAYE VGQEVSVDTF VAGDVIDVTG VSKGKGFQGA IKRHGQSRGP MSHGSHFHRA PGSVGMASDA S RVFKGQKM ...String:
TKGILGRKIG MTQVFGENGE LIPVTVVEAK ENVVLQKKTV EVDGYNAIQV GFEDKKAYKK DAKSNKYANK PAEGHAKKAD AAPKRFIRE FRNVDVDAYE VGQEVSVDTF VAGDVIDVTG VSKGKGFQGA IKRHGQSRGP MSHGSHFHRA PGSVGMASDA S RVFKGQKM PGRMGGNTVT VQNLEVVQVD TENKVILVKG NVPGPKKGLV EIRTSIK

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #22: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 22.293424 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString: NYDVLKLDGT KSGSIELSDA VFGIEPNNSV LFEAINLQRA SLRQGTHAVK NRSAVSGGGR KPWKQKGTGR ARQGTIRAPQ WRGGGIVFG PTPRSYAYKM PKKMRRLALR SALSFKAQEN GLTVVDAFNF EAPKTKEFKN VLSTLEQPKK VLVVTENEDV N VELSARNI ...String:
NYDVLKLDGT KSGSIELSDA VFGIEPNNSV LFEAINLQRA SLRQGTHAVK NRSAVSGGGR KPWKQKGTGR ARQGTIRAPQ WRGGGIVFG PTPRSYAYKM PKKMRRLALR SALSFKAQEN GLTVVDAFNF EAPKTKEFKN VLSTLEQPKK VLVVTENEDV N VELSARNI PGVQVTTAQG LNVLDITNAD SLVITEAAAK KVEEVLG

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #23: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 18.644643 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
RLKEKFNTEV TENLMKKFNY SSVMEVPKID KIVVNMGVGD AVQNSKVLDN AVEELELITG QKPLVTKAKK SIATFRLREG MPIGAKVTL RGERMYEFLD KLISVSLPRV RDFQGVSKKA FDGRGNYTLG VKEQLIFPEI DYDKVSKVRG MDIVIVTTAN T DEEAR

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #24: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 19.400051 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
SRVGKKIIDI PSDVTVTFDG NHVTVKGPKG ELSRTLNERM TFKQEENTIE VVRPSDSKED RTNHGTTRAL LNNMVQGVSQ GYVKVLELV GVGYRAQMQG KDLILNVGYS HPVEIKAEEN ITFSVEKNTV VKVEGISKEQ VGALASNIRS VRPPEPYKGK G IRYQGEYV RRKEGK

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #25: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 16.359427 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
MRQTFMANES NIERKWYVID AEGQTLGRLS SEVASILRGK NKVTYTPHVD TGDYVIVINA SKIEFTGNKE TDKVYYRHSN HPGGIKSIT AGELRRTNPE RLIENSIKGM LPSTRLGEKQ GKKLFVYGGA EHPHAAQQPE NYELRG

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #26: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.157342 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
MIQQETRLKV ADNSGAREVL TIKVLGGSGR KTANIGDVIV CTVKNATPGG VVKKGDVVKA VIVRTKSGVR RNDGSYIKFD ENACVIIRD DKGPRGTRIF GPVARELREG NFMKIVSLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #27: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 15.515731 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
MKLHELKPAE GSRKERNRVG RGVATGNGKT SGRGHKGQKA RSGGGVRPGF EGGQLPLFRR LPKRGFTNIN RKEYAIVNLD QLNKFEDGT EVTPALLVES GVVKNEKSGI KILGNGSLDK KLTVKAHKFS ASAAEAIDAK GGAHEV

UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #28: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 15.486276 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
MLLPKRVKYR RQHRPKTTGR SKGGNYVTFG EFGLQATTTS WITSRQIESA RIAMTRYMKR GGKVWIKIFP HTPYTKKPLE VRMGAGKGA VEGWIAVVKP GRILFEVAGV SEEVAREALR LASHKLPVKT KFVKREE

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #29: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.42035 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
RKLGRTSDQR KAMLRDLATS LIISERIETT EARAKEVRSV VEKLITLGKK GDLASRRNAA KTLRNVEILN EDETTQTALQ KLFGEIAER YTERQGGYTR ILKQGPRRGD GAESVIIELV

UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #30: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 12.461404 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
MISKIDKNKV RLKRHARVRT NLSGTAEKPR LNVYRSNKHI YAQIIDDNKG VTLAQASSKD SDIATTATKV ELATKVGEAI AKKAADKGI KEIVFDRGGY LYHGRVKALA EAAR

UniProtKB: UNIPROTKB: A0A6K6IAA8

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Macromolecule #31: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.459919 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
PRVKGGTVTR ARRKKTIKLA KGYFGSKHTL YKVAKQQVMK SGQYAFRDRR QRKRDFRKLW ITRINAAARQ HEMSYSRLMN GLKKAGIDI NRKMLSEIAI SDEKAFAQLV TKAKDAL

UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #32: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 11.354081 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
MFAIIETGGK QIKVEEGQEI FVEKLDVNEG DTFTFDKVLF VGGDSVKVGA PTVEGATVTA TVNKQGRGKK ITVFTYKRRK NSKRKKGHR QPYTKLTIDK INA

UniProtKB: Large ribosomal subunit protein bL21

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Macromolecule #33: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 12.328358 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
MEAKAVARTI RIAPRKVRLV LDLIRGKNAA EAIAILKLTN KASSPVIEKV LMSALANAEH NYDMNTDELV VKEAYANEGP TLKRFRPRA QGRASAINKR TSHITIVVSD GKE

UniProtKB: UNIPROTKB: A0A6I4H7Y4

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Macromolecule #34: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.3801 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
EARDILKRPV ITEKSSEAMA EDKYTFDVDT RVNKTQVKMA VEEIFNVKVA SVNIMNYKPK KKRMGRYQGY TNKRRKAIVT LKEGSIDLF

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #35: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 11.316205 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
HIKKGDNVKV IAGKDKGKEG KVIATLPKKD RVVVEGVNIM KKHQKPTQLN PEGGILETEA AIHVSNVQLL DPKTNEPTRV GYKFVDGKK VRIAKKSGEE IKSN

UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #36: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.391122 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
SLKSIIRQGK QTRSDLKQLR KSGKVPAVVY GYGTKNVSVK VDEVEFIKVI REVGRNGVIE LGVGSKTIKV MVADYQFDPL KNQITHIDF LAIN

UniProtKB: UNIPROTKB: A0A6H3X640

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Macromolecule #37: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 8.911099 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
KKGVSSTKNG RDSESKRLGA KRADGQFVTG GSILYRQRGT KIYPGENVGR GGDDTLFAKI DGVVKFERKG RDKKQVSVYA VA

UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #38: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 5.499432 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
ASTGNRRSHA LNSTKRRWNA NLQKVRILVD GKPKKVWVSA RALKSGKVT

UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #39: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 7.331325 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
EIRDLTTSEI EEQIKSSKEE LFNLRFQLAT GQLEETARIR TVRKTIARLK TVAREREIEQ SK

UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #40: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.305308 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
AKLQITLTRS VIGRPETQRK TVEALGLKKT NSSVVVEDNP AIRGQINKVK HLVTVEE

UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #41: 50S ribosomalprotein L32p

MacromoleculeName: 50S ribosomalprotein L32p / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 5.406392 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
AVPKRRTSKT RKNKRRTHFK ISVPGMTECP NCGEYKLSHR VCKNCGS

UniProtKB: UNIPROTKB: A0A6K0ZQ16

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Macromolecule #42: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 5.684561 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
RVNVTLACTE CGDRNYITTK NKRNNPERIE MKKYCPRLNK YTLHRET

UniProtKB: Large ribosomal subunit protein bL33

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Macromolecule #43: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 5.252369 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
VKRTYQPNKR KHSKVHGFRK RMSTKNGRKV LARRRRKGRK VLS

UniProtKB: Large ribosomal subunit protein bL34

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Macromolecule #44: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.985402 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
PKMKTHRGAA KRVKRTASGQ LKRSRAFTSH LFANKSTKQK RQLRKARLVS KSDMKRVKQL

UniProtKB: UNIPROTKB: A0A6J8FVP5

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Macromolecule #45: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 4.318422 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
MKVRPSVKPI CEKCKVIKRK GKVMVICENP KHKQRQG

UniProtKB: Large ribosomal subunit protein bL36

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Macromolecule #46: 50S ribosomal protein L31 type B

MacromoleculeName: 50S ribosomal protein L31 type B / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 8.603602 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
MKQGIHPEYH QVIFLDTTTN FKFLSGSTKT SSEMMEWEDG KEYPVIRLDI SSDSHPFYTG RQKFAAADGR VERF

UniProtKB: Large ribosomal subunit protein bL31B

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.6
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 200
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 47.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 494375
Startup modelType of model: PDB ENTRY
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.11 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 123520
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE

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