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- EMDB-11900: Staphylococcus aureus 50S after 30 minutes incubation at 37C -

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Basic information

Entry
Database: EMDB / ID: EMD-11900
TitleStaphylococcus aureus 50S after 30 minutes incubation at 37C
Map data
Sample
  • Complex: 50S ribosomal subunitProkaryotic large ribosomal subunit
    • Protein or peptide: x 27 types
    • RNA: x 2 types
  • Ligand: x 1 types
Function / homology
Function and homology information


large ribosomal subunit / ribosomal large subunit assembly / 5S rRNA binding / transferase activity / tRNA binding / ribosome / rRNA binding / structural constituent of ribosome / translation / cytoplasm
Similarity search - Function
Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein TL5, C-terminal domain / Ribosomal protein L25, C-terminal / Ribosomal protein L16 signature 1. / Ribosomal protein L6 signature 1. / Ribosomal protein L6, conserved site / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. ...Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein TL5, C-terminal domain / Ribosomal protein L25, C-terminal / Ribosomal protein L16 signature 1. / Ribosomal protein L6 signature 1. / Ribosomal protein L6, conserved site / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L32p, bacterial type / Ribosomal protein L36 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L28 / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35 signature. / Ribosomal protein L35, conserved site / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19 signature. / Ribosomal protein L19, conserved site / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L6, bacterial-type / Ribosomal protein L20 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L36 / Ribosomal protein L14P, bacterial-type / Ribosomal protein L2, bacterial/organellar-type / Ribosomal L28 family / Ribosomal protein L35 / Ribosomal protein L33 / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L28/L24 / Ribosomal protein L33 / Ribosomal protein L33 superfamily / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / L28p-like / Ribosomal protein L16 / Ribosomal protein L20 / Ribosomal L32p protein family / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L30, bacterial-type / Ribosomal protein L34 / Ribosomal protein L34 / Ribosomal protein L19 superfamily / Ribosomal protein L21 / Ribosomal protein L19 / Ribosomal protein L19 / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L32p / Ribosomal protein L17 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal protein L13, bacterial-type / Ribosomal protein L3, bacterial/organelle-type / Ribosomal protein L15, bacterial-type / 50S ribosomal protein uL4 / Ribosomal protein L5 signature. / Ribosomal protein L5, conserved site / Ribosomal protein L2, conserved site / Ribosomal protein L2 signature. / Ribosomal protein L5 / Ribosomal protein L5, N-terminal / Ribosomal protein L5, C-terminal / ribosomal L5P family C-terminus / Ribosomal protein L5 / Ribosomal protein L5 domain superfamily / Ribosomal protein L15 signature. / Ribosomal protein L15, conserved site / Ribosomal protein L6, alpha-beta domain / Ribosomal protein L6 / Ribosomal protein L6 / Ribosomal protein L6, alpha-beta domain superfamily / Ribosomal protein L13, conserved site / Ribosomal protein L13 signature. / Ribosomal protein L2, domain 3 / Ribosomal protein L14 signature. / Ribosomal protein L14P, conserved site / Ribosomal L29 protein / Ribosomal protein L29/L35 / Ribosomal protein L29/L35 superfamily / Ribosomal protein L10e/L16 superfamily / Ribosomal protein L16p/L10e
Similarity search - Domain/homology
50S ribosomal protein L6 / 50S ribosomal protein L3 / 50S ribosomal protein L13 / 50S ribosomal protein L23 / 50S ribosomal protein L18 / 50S ribosomal protein L4 / 50S ribosomal protein L5 / 50S ribosomal protein L34 / 50S ribosomal protein L21 / 50S ribosomal protein L2 ...50S ribosomal protein L6 / 50S ribosomal protein L3 / 50S ribosomal protein L13 / 50S ribosomal protein L23 / 50S ribosomal protein L18 / 50S ribosomal protein L4 / 50S ribosomal protein L5 / 50S ribosomal protein L34 / 50S ribosomal protein L21 / 50S ribosomal protein L2 / : / : / 50S ribosomal protein L14 / 50S ribosomal protein L32 / 50S ribosomal protein L25 / 50S ribosomal protein L29 / 50S ribosomal protein L20 / 50S ribosomal protein L16 / 50S ribosomal protein L33 / 50S ribosomal protein L19 / 50S ribosomal protein L28 / 50S ribosomal protein L27 / 50S ribosomal protein L35 / 50S ribosomal protein L36 / 50S ribosomal protein L15 / 50S ribosomal protein L17 / 50S ribosomal protein L30
Similarity search - Component
Biological speciesStaphylococcus aureus (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.48 Å
AuthorsCimicata G / Bashan A / Yonath A
Funding support1 items
OrganizationGrant numberCountry
European Research Council (ERC)322581
CitationJournal: To Be Published
Title: Staphylococcus aureus 50S after 30 minutes incubation a 37C
Authors: Cimicata G / Bashan A / Yonath A
History
DepositionOct 27, 2020-
Header (metadata) releaseNov 17, 2021-
Map releaseNov 17, 2021-
UpdateNov 17, 2021-
Current statusNov 17, 2021Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0037
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.0037
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-7asm
  • Surface level: 0.0037
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_11900.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.86 Å/pix.
x 360 pix.
= 308.88 Å
0.86 Å/pix.
x 360 pix.
= 308.88 Å
0.86 Å/pix.
x 360 pix.
= 308.88 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.858 Å
Density
Contour LevelBy AUTHOR: 0.0037 / Movie #1: 0.0037
Minimum - Maximum-0.029684417 - 0.08533615
Average (Standard dev.)-6.4310785e-05 (±0.0033578738)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 308.88 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.8580.8580.858
M x/y/z360360360
origin x/y/z0.0000.0000.000
length x/y/z308.880308.880308.880
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS360360360
D min/max/mean-0.0300.085-0.000

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Supplemental data

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Additional map: #1

Fileemd_11900_additional_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Sample components

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Entire : 50S ribosomal subunit

EntireName: 50S ribosomal subunitProkaryotic large ribosomal subunit
Components
  • Complex: 50S ribosomal subunitProkaryotic large ribosomal subunit
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L4
    • RNA: 23S rRNA23S ribosomal RNA
    • RNA: 5S rRNA5S ribosomal RNA
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L25
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L33
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L36
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: 50S ribosomal protein L17
  • Ligand: MAGNESIUM ION

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Supramolecule #1: 50S ribosomal subunit

SupramoleculeName: 50S ribosomal subunit / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#29
Source (natural)Organism: Staphylococcus aureus (bacteria)

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Macromolecule #1: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.104381 KDa
SequenceString:
TNHKLIEAVT KSQLRTDLPS FRPGDTLRVH VRIIEGTRER IQVFEGVVIK RRGGGVSETF TVRKISSGVG VERTFPLHTP KIEKIEVKR RGKVRRAKLY YLRSLRGKAA RIQEI

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Macromolecule #2: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 22.364504 KDa
SequenceString: ANYDVLKLDG TKSGSIELSD AVFGIEPNNS VLFEAINLQR ASLRQGTHAV KNRSAVSGGG RKPWKQKGTG RARQGTIRAP QWRGGGIVF GPTPRSYAYK MPKKMRRLAL RSALSFKAQE NGLTVVDAFN FEAPKTKEFK NVLSTLEQPK KVLVVTENED V NVELSARN ...String:
ANYDVLKLDG TKSGSIELSD AVFGIEPNNS VLFEAINLQR ASLRQGTHAV KNRSAVSGGG RKPWKQKGTG RARQGTIRAP QWRGGGIVF GPTPRSYAYK MPKKMRRLAL RSALSFKAQE NGLTVVDAFN FEAPKTKEFK NVLSTLEQPK KVLVVTENED V NVELSARN IPGVQVTTAQ GLNVLDITNA DSLVITEAAA KKVEEVLG

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Macromolecule #5: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 29.827607 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString: AIKKYKPITN GRRNMTSLDF AEITKTTPEK SLLKPLPKKA GRNNQGKLTV RHHGGGHKRQ YRVIDFKRNK DGINAKVDSI QYDPNRSAN IALVVYADGE KRYIIAPKGL EVGQIVESGA EADIKVGNAL PLQNIPVGTV VHNIELKPGK GGQIARSAGA S AQVLGKEG ...String:
AIKKYKPITN GRRNMTSLDF AEITKTTPEK SLLKPLPKKA GRNNQGKLTV RHHGGGHKRQ YRVIDFKRNK DGINAKVDSI QYDPNRSAN IALVVYADGE KRYIIAPKGL EVGQIVESGA EADIKVGNAL PLQNIPVGTV VHNIELKPGK GGQIARSAGA S AQVLGKEG KYVLIRLRSG EVRMILSTCR ATIGQVGNLQ HELVNVGKAG RSRWKGIRPT VRGSVMNPND HPHGGGEGRA PI GRPSPMS PWGKPTLGKK TRRGKKSSDK LIVRGRK

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Macromolecule #6: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 19.501154 KDa
SequenceString:
SRVGKKIIDI PSDVTVTFDG NHVTVKGPKG ELSRTLNERM TFKQEENTIE VVRPSDSKED RTNHGTTRAL LNNMVQGVSQ GYVKVLELV GVGYRAQMQG KDLILNVGYS HPVEIKAEEN ITFSVEKNTV VKVEGISKEQ VGALASNIRS VRPPEPYKGK G IRYQGEYV RRKEGKT

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Macromolecule #7: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 16.359427 KDa
SequenceString:
MRQTFMANES NIERKWYVID AEGQTLGRLS SEVASILRGK NKVTYTPHVD TGDYVIVINA SKIEFTGNKE TDKVYYRHSN HPGGIKSIT AGELRRTNPE RLIENSIKGM LPSTRLGEKQ GKKLFVYGGA EHPHAAQQPE NYELRG

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Macromolecule #8: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 15.62889 KDa
SequenceString:
MKLHELKPAE GSRKERNRVG RGVATGNGKT SGRGHKGQKA RSGGGVRPGF EGGQLPLFRR LPKRGFTNIN RKEYAIVNLD QLNKFEDGT EVTPALLVES GVVKNEKSGI KILGNGSLDK KLTVKAHKFS ASAAEAIDAK GGAHEVI

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Macromolecule #9: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 15.599435 KDa
Recombinant expressionOrganism: Staphylococcus aureus (bacteria)
SequenceString:
MLLPKRVKYR RQHRPKTTGR SKGGNYVTFG EFGLQATTTS WITSRQIESA RIAMTRYMKR GGKVWIKIFP HTPYTKKPLE VRMGAGKGA VEGWIAVVKP GRILFEVAGV SEEVAREALR LASHKLPVKT KFVKREEL

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Macromolecule #10: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.124093 KDa
SequenceString:
MISKIDKNKV RLKRHARVRT NLSGTAEKPR LNVYRSNKHI YAQIIDDNKG VTLAQASSKD SDIATTATKV ELATKVGEAI AKKAADKGI KEIVFDRGGY LYHGRVKALA EAARESGLEF

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Macromolecule #11: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.459919 KDa
SequenceString:
PRVKGGTVTR ARRKKTIKLA KGYFGSKHTL YKVAKQQVMK SGQYAFRDRR QRKRDFRKLW ITRINAAARQ HEMSYSRLMN GLKKAGIDI NRKMLSEIAI SDEKAFAQLV TKAKDAL

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Macromolecule #12: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 11.354081 KDa
SequenceString:
MFAIIETGGK QIKVEEGQEI FVEKLDVNEG DTFTFDKVLF VGGDSVKVGA PTVEGATVTA TVNKQGRGKK ITVFTYKRRK NSKRKKGHR QPYTKLTIDK INA

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Macromolecule #13: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.3801 KDa
SequenceString:
EARDILKRPV ITEKSSEAMA EDKYTFDVDT RVNKTQVKMA VEEIFNVKVA SVNIMNYKPK KKRMGRYQGY TNKRRKAIVT LKEGSIDLF

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Macromolecule #14: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 14
Details: MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKHQKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIKSNN
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 11.447401 KDa
SequenceString:
MHIKKGDNVK VIAGKDKGKE GKVIATLPKK DRVVVEGVNI MKKHQKPTQL NPEGGILETE AAIHVSNVQL LDPKTNEPTR VGYKFVDGK KVRIAKKSGE EIKSN

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Macromolecule #15: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 10.4622 KDa
SequenceString:
ASLKSIIRQG KQTRSDLKQL RKSGKVPAVV YGYGTKNVSV KVDEVEFIKV IREVGRNGVI ELGVGSKTIK VMVADYQFDP LKNQITHID FLAIN

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Macromolecule #16: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 8.595688 KDa
SequenceString:
VSSTKNGRDS ESKRLGAKRA DGQFVTGGSI LYRQRGTKIY PGENVGRGGD DTLFAKIDGV VKFERKGRDK KQVSVYAVA

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Macromolecule #17: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 7.845942 KDa
SequenceString:
KAKEIRDLTT SEIEEQIKSS KEELFNLRFQ LATGQLEETA RIRTVRKTIA RLKTVARERE IEQSKAN

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Macromolecule #18: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 7.461992 KDa
SequenceString:
PKMKTHRGAA KRVKRTASGQ LKRSRAFTSH LFANKSTKQK RQLRKARLVS KSDMKRVKQL LAYK

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Macromolecule #19: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 5.684561 KDa
SequenceString:
RVNVTLACTE CGDRNYITTK NKRNNPERIE MKKYCPRLNK YTLHRET

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Macromolecule #20: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 5.252369 KDa
SequenceString:
VKRTYQPNKR KHSKVHGFRK RMSTKNGRKV LARRRRKGRK VLS

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Macromolecule #21: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 4.318422 KDa
SequenceString:
MKVRPSVKPI CEKCKVIKRK GKVMVICENP KHKQRQG

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Macromolecule #22: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 23.199545 KDa
SequenceString: TKGILGRKIG MTQVFGENGE LIPVTVVEAK ENVVLQKKTV EVDGYNAIQV GFEDKKAYKK DAKSNKYANK PAEGHAKKAD AAPKRFIRE FRNVDVDAYE VGQEVSVDTF VAGDVIDVTG VSKGKGFQGA IKRHGQSRGP MSHGSHFHRA PGSVGMASDA S RVFKGQKM ...String:
TKGILGRKIG MTQVFGENGE LIPVTVVEAK ENVVLQKKTV EVDGYNAIQV GFEDKKAYKK DAKSNKYANK PAEGHAKKAD AAPKRFIRE FRNVDVDAYE VGQEVSVDTF VAGDVIDVTG VSKGKGFQGA IKRHGQSRGP MSHGSHFHRA PGSVGMASDA S RVFKGQKM PGRMGGNTVT VQNLEVVQVD TENKVILVKG NVPGPKKGLV EIRTSIK

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Macromolecule #23: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 17.651479 KDa
SequenceString:
KFNYSSVMEV PKIDKIVVNM GVGDAVQNSK VLDNAVEELE LITGQKPLVT KAKKSIATFR LREGMPIGAK VTLRGERMYE FLDKLISVS LPRVRDFQGV SKKAFDGRGN YTLGVKEQLI FPEIDYDKVS KVRGMDIVIV TTANTDEEAR ELLANFGMP

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Macromolecule #24: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.157342 KDa
SequenceString:
MIQQETRLKV ADNSGAREVL TIKVLGGSGR KTANIGDVIV CTVKNATPGG VVKKGDVVKA VIVRTKSGVR RNDGSYIKFD ENACVIIRD DKGPRGTRIF GPVARELREG NFMKIVSLAP EVL

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Macromolecule #25: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 12.328358 KDa
SequenceString:
MEAKAVARTI RIAPRKVRLV LDLIRGKNAA EAIAILKLTN KASSPVIEKV LMSALANAEH NYDMNTDELV VKEAYANEGP TLKRFRPRA QGRASAINKR TSHITIVVSD GKE

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Macromolecule #26: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 5.499432 KDa
SequenceString:
ASTGNRRSHA LNSTKRRWNA NLQKVRILVD GKPKKVWVSA RALKSGKVT

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Macromolecule #27: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 6.434487 KDa
SequenceString:
AKLQITLTRS VIGRPETQRK TVEALGLKKT NSSVVVEDNP AIRGQINKVK HLVTVEEK

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Macromolecule #28: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 5.569565 KDa
SequenceString:
AVPKRRTSKT RKNKRRTHFK ISVPGMTECP NCGEYKLSHR VCKNCGSY

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Macromolecule #29: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 13.583523 KDa
SequenceString:
YRKLGRTSDQ RKAMLRDLAT SLIISERIET TEARAKEVRS VVEKLITLGK KGDLASRRNA AKTLRNVEIL NEDETTQTAL QKLFGEIAE RYTERQGGYT RILKQGPRRG DGAESVIIEL V

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Macromolecule #3: 23S rRNA

MacromoleculeName: 23S rRNA / type: rna / ID: 3
Details: ...Details: GAUUAAGUUAUUAAGGGCGCACGGUGGAUGCCUUGGCACUAGAAGCCGAUGAAGGACGUUACUAACGACGAUAUGCUUUGGGGAGCUGUAAGCUUUGAUCCAGAGAUUUCCGAAUGGGGAAACCCAGCAUGAGUUAUGUCAUGUUAUCGAUAUGUGAAUACAUAGCAUAUCAGAAGGCACACCCGGAGAACUGAAACAUCUUAGUACCCGGAGGAAGAGAAAGAAAAUUCGAUUCCCUUAGUAGCGGCGAGCGAAACGGGAAGAGCCCAAACCAACAAGCUUGCUUGUUGGGGUUGUAGGACACUCUGUACGGAGUUACAAAGGACGACAUUAGACGAAUCAUCUGGAAAGAUGAAUCAAAGAAGGUAAUAAUCCUGUAGUCGAAAAUGUUGUCUCUCUUGAGUGGAUCCUGAGUACGACGGAGCACGUGAAAUUCCGUCGGAAUCUGGGAGGACCAUCUCCUAAGGCUAAAUACUCUCUAGUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGUGAAAAGCACCCCGGAAGGGGAGUGAAAUAGAACCUGAAACCGUGUGCUUACAAGUAGUCAGAGCCCGUUAAUGGGUGAUGGCGUGCCUUUUGUAGAAUGAACCGGCGAGUUACGAUUUGAUGCAAGGUUAAGCAGUAAAUGUGGAGCCGUAGCGAAAGCGAGUCUGAAUAGGGCGUUUAGUAUUUGGUCGUAGACCCGAAACCAGGUGAUCUACCCUUGGUCAGGUUGAAGUUCAGGUAACACUGAAUGGAGGACCGAACCGACUUACGUUGAAAAGUGAGCGGAUGAACUGAGGGUAGCGGAGAAAUUCCAAUCGAACCUGGAGAUAGCUGGUUCUCUCCGAAAUAGCUUUAGGGCUAGCCUCAAGUGAUGAUUAUUGGAGGUAGAGCACUGUUUGGACGAGGGGCCCCUCUCGGGUUACCGAAUUCAGACAAACUCCGAAUGCCAAUUAAUUUAACUUGGGAGUCAGAACAUGGGUGAUAAGGUCCGUGUUCGAAAGGGAAACAGCCCAGACCACCAGCUAAGGUCCCAAAAUAUAUGUUAAGUGGAAAAGGAUGUGGCGUUGCCCAGACAACUAGGAUGUUGGCUUAGAAGCAGCCAUCAUUUAAAGAGUGCGUAAUAGCUCACUAGUCGAGUGACACUGCGCCGAAAAUGUACCGGGGCUAAACAUAUUACCGAAGCUGUGGAUUGUCCUUUGGACAAUGGUAGGAGAGCGUUCUAAGGGCGUUGAAGCAUGAUCGUAAGGACAUGUGGAGCGCUUAGAAGUGAGAAUGCCGGUGUGAGUAGCGAAAGACGGGUGAGAAUCCCGUCCACCGAUUGACUAAGGUUUCCAGAGGAAGGCUCGUCCGCUCUGGGUUAGUCGGGUCCUAAGCUGAGGCCGACAGGCGUAGGCGAUGGAUAACAGGUUGAUAUUCCUGUACCACCUAUAAUCGUUUUAAUCGAUGGGGGGACGCAGUAGGAUAGGCGAAGCGUGCGAUUGGAUUGCACGUCUAAGCAGUAAGGCUGAGUAUUAGGCAAAUCCGGUACUCGUUAAGGCUGAGCUGUGAUGGGGAGAAGACAUUGAGUCUUCGAGUCGUUGAUUUCACACUGCCGAGAAAAGCCUCUAGAUAGAAAAUAGGUGCCCGUACCGCAAACCGACACAGGUAGUCAAGAUGAGAAUUCUAAGGUGAGCGAGCGAACUCUCGUUAAGGAACUCGGCAAAAUGACCCCGGUAACUUCGGGAGAAGGGGUGCUCUUUAGGGUUAACGCCCAGAAGAGCCGCAGUGAAUAGGCCCAAGCGACUGUUUAUCAAAAACACAGGUCUCUGCUAAACCGUAAGGUGAUGUAUAGGGGCUGACGCCUGCCCGGUGCUGGAAGGUUAAGAGGAGUGGUUAGCUUCUGCGAAGCUACGAAUCGAAGCCCCAGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGAAAGGCGUAACGAUUUGGGCACUGUCUCAACGAGAGACUCGGUGAAAUCAUAGUACCUGUGAAGAUGCAGGUUACCCGCGACAGGACGGAAAGACCCCGUGGAGCUUUACUGUAGCCUGAUAUUGAAAUUCGGCACAGCUUGUACAGGAUAGGUAGGAGCCUUUGAAACGUGAGCGCUAGCUUACGUGGAGGCGCUGGUGGGAUACUACCCUAGCUGUGUUGGCUUUCUAACCCGCACCACUUAUCGUGGUGGGAGACAGUGUCAAGCGGGCAGUUUGACUGGGGCGGUCGCCUCCUAAAAGGUAACGGAGGCGCUCAAAGGUUCCCUCAGAAUGGUUGGAAAUCAUUCAUAGAGUGUAAAGGCAUAAGGGAGCUUGACUGCGAGACCUACAAGUCGAGCAGGGUCGAAAGACGGACUUAGUGAUCCGGUGGUUCCGCAUGGAAGGGCCAUCGCUCAACGGAUAAAAGCUACCCCGGGGAUAACAGGCUUAUCUCCCCCAAGAGUUCACAUCGACGGGGAGGUUUGGCACCUCGAUGUCGGCUCAUCGCAUCCUGGGGCUGUAGUCGGUCCCAAGGGUUGGGCUGUUCGCCCAUUAAAGCGGUACGCGAGCUGGGUUCAGAACGUCGUGAGACAGUUCGGUCCCUAUCCGUCGUGGGCGUAGGAAAUUUGAGAGGAGCUGUCCUUAGUACGAGAGGACCGGGAUGGACAUACCUCUGGUGUACCAGUUGUCGUGCCAACGGCAUAGCUGGGUAGCUAUGUGUGGACGGGAUAAGUGCUGAAAGCAUCUAAGCAUGAAGCCCCCCUCAAGAUGAGAUUUCCCAACUUCGGUUAUAAGAUCCCUCAAAGAUGAUGAGGUUAAUAGGUUCGAGGUGGAAGCAUGGUGACAUGUGGAGCUGACGAAUACUAAUCGAUCGAAGACUUAAUCAA
Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 946.738688 KDa
SequenceString: GAUUAAGUUA UUAAGGGCGC ACGGUGGAUG CCUUGGCACU AGAAGCCGAU GAAGGACGUU ACUAACGACG AUAUGCUUUG GGGAGCUGU AAGUAAGCUU UGAUCCAGAG AUUUCCGAAU GGGGAAACCC AGCAUGAGUU AUGUCAUGUU AUCGAUAUGU G AAUACAUA ...String:
GAUUAAGUUA UUAAGGGCGC ACGGUGGAUG CCUUGGCACU AGAAGCCGAU GAAGGACGUU ACUAACGACG AUAUGCUUUG GGGAGCUGU AAGUAAGCUU UGAUCCAGAG AUUUCCGAAU GGGGAAACCC AGCAUGAGUU AUGUCAUGUU AUCGAUAUGU G AAUACAUA GCAUAUCAGA AGGCACACCC GGAGAACUGA AACAUCUUAG UACCCGGAGG AAGAGAAAGA AAAUUCGAUU CC CUUAGUA GCGGCGAGCG AAACGGGAAG AGCCCAAACC AACAAGCUUG CUUGUUGGGG UUGUAGGACA CUCUAUACGG AGU UACAAA GGACGACAUU AGACGAAUCA UCUGGAAAGA UGAAUCAAAG AAGGUAAUAA UCCUGUAGUC GAAAAUGUUG UCUC UCUUG AGUGGAUCCU GAGUACGACG GAGCACGUGA AAUUCCGUCG GAAUCUGGGA GGACCAUCUC CUAAGGCUAA AUACU CUCU AGUGACCGAU AGUGAACCAG UACCGUGAGG GAAAGGUGAA AAGCACCCCG GAAGGGGAGU GAAAUAGAAC CUGAAA CCG UGUGCUUACA AGUAGUCAGA GCCCGUUAAU GGGUGAUGGC GUGCCUUUUG UAGAAUGAAC CGGCGAGUUA CGAUUUG AU GCAAGGUUAA GCAGUAAAUG UGGAGCCGUA GCGAAAGCGA GUCUGAAUAG GGCGUUUAGU AUUUGGUCGU AGACCCGA A ACCAGGUGAU CUACCCUUGG UCAGGUUGAA GUUCAGGUAA CACUGAAUGG AGGACCGAAC CGACUUACGU (5MU)GAAA AGUG AGCGGAUGAA CUGAGGGUAG CGGAGAAAUU CCAAUCGAAC CUGGAGAUAG CUGGUUCUCU CCGAAAUAGC UUUAGG GCU AGCCUCAAGU GAUGAUUAUU GGAGGUAGAG CACUGUUUGG ACGAGGGGCC CCUCUCGGGU UACCGAAUUC AGACAAA CU CCGAAUGCCA AUUAAUUUAA CUUGGGAGUC AGAACAUGGG UGAUAAGGUC CGUGUUCGAA AGGGAAACAG CCCAGACC A CCAGCUAAGG UCCCAAAAUA UAUGUUAAGU GGAAAAGGAU GUGGCGUUGC CCAGACAACU AGGAUGUUGG CUUAGAAGC AGCCAUCAUU UAAAGAGUGC GUAAUAGCUC ACUAGUCGAG UGACACUGCG CCGAAAAUGU ACCGGGGCUA AACAUAUUAC CGAAGCUGU GGAUUGUCCU UUGGACAAUG GUAGGAGAGC GUUCUAAGGG CGUUGAAGCA UGAUCGUAAG GACAUGUGGA G CGCUUAGA AGUGAGAAUG CCGGUGUGAG UAGCGAAAGA CGGGUGAGAA UCCCGUCCAC CGAUUGACUA AGGUUUCCAG AG GAAGGCU CGUCCGCUCU GGGUUAGUCG GGUCCUAAGC UGAGGCCGAC AGGCGUAGGC GAUGGAUAAC AGGUUGAUAU UCC UGUACC ACCUAUAAUC GUUUUAAUCG AUGGGGGGAC GCAGUAGGAU AGGCGAAGCG UGCGAUUGGA UUGCACGUCU AAGC AGUAA GGCUGAGUAU UAGGCAAAUC CGGUACUCGU UAAGGCUGAG CUGUGAUGGG GAGAAGACAU UGUGUCUUCG AGUCG UUGA UUUCACACUG CCGAGAAAAG CCUCUAGAUA GAAAAUAGGU GCCCGUACCG CAAACCGACA CAGGUAGUCA AGAUGA GAA UUCUAAGGUG AGCGAGCGAA CUCUCGUUAA GGAACUCGGC AAAAUGACCC CGUAACUUCG GGAGAAGGGG UGCUCUU UA GGGUUAACGC CCAGAAGAGC CGCAGUGAAU AGGCCCAAGC GACUGUUUAU CAAAAACACA GGUCUCUGCU AAACCGUA A GGUGAUGUAU AGGGGCUGAC GCCUGCCCGG UGCUGGAAGG UUAAGAGGAG UGGUUAGCUU CUGCGAAGCU ACGAAUCGA AGCCCCAGUA AACGGCGGCC GUAACUAUAA CGGUCCUAAG GUAGCGAAA(5MU) UCCUUGUCGG GUAAGUUCCG ACCCGC ACG AAAGGCGUAA CGAUUUGGGC ACUGUCUCAA CGAGAGACUC GGUGAAAUCA UAGUACCUGU GAAGAUGCAG GUUACCC GC GACAGGACGG AAAGACCCCG UGGAGCUUUA CUGUAGCCUG AUAUUGAAAU UCGGCACAGC UUGUACAGGA UAGGUAGG A GCCUUUGAAA CGUGAGCGCU AGCUUACGUG GAGGCGCUGG UGGGAUACUA CCCUAGCUGU GUUGGCUUUC UAACCCGCA CCACUUAUCG UGGUGGGAGA CAGUGUCAGG CGGGCAGUUU GACUGGGGCG GUCGCCUCCU AAAAGGUAAC GGAGGCGCUC AAAGGUUCC CUCAGAAUGG UUGGAAAUCA UUCAUAGAGU GUAAAGGCAU AAGGGAGCUU GACUGCGAGA CCUACAAGUC G AGCAGGGU CGAAAGACGG ACUUAGUGAU CCGGUGGUUC CGCAUGGAAG GGCCAUCGCU CAACGGAUAA AAGCUACCCC GG GGAUAAC AGGCUUAUCU CCCCCAAGAG UUCACAUCGA CGGGGAGGUU UGGCACCUCG (2MA)UGUCGGCUC AUCGCAUCC UGGGGCUGUA GUCGGUCCCA AGGGUUGGGC UGUUCGCCCA UUAAAGCGGU ACGCGAGCUG GGUUCAGAAC GUCGUGAGAC AGUUCGGUC CCUAUCCGUC GUGGGCGUAG GAAAUUUGAG AGGAGCUGUC CUUAGUACGA GAGGACCGGG AUGGACAUAC C UCUGGUGU ACCAGUUGUC GUGCCAACGG CAUAGCUGGG UAGCUAUGUG UGGACGGGAU AAGUGCUGAA AGCAUCUAAG CA UGAAGCC CCCCUCAAGA UGAGAUUUCC CAACUUCGGU UAUAAGAUCC CUCAAAGAUG AUGAGGUUAA UAGGUUCGAG GUG GAAGCA UGGUGACAUG UGGAGCUGAC GAAUACUAAU CGAUCGAAGA CUUAAUCAA

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Macromolecule #4: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 4 / Number of copies: 1
Source (natural)Organism: Staphylococcus aureus (bacteria)
Molecular weightTheoretical: 36.974945 KDa
SequenceString:
UCUGGUGACU AUAGCAAGGA GGUCACACCU GUUCCCAUGC CGAACACAGA AGUUAAGCUC CUUAGCGUCG AUGGUAGUCG AACUUACGU UCCGCUAGAG UAGAACGUUG CCAGGC

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Macromolecule #30: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 30 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.6
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 200
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 47.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.48 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 198422

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