+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-11900 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Staphylococcus aureus 50S after 30 minutes incubation at 37C | |||||||||
Map data | ||||||||||
Sample |
| |||||||||
Function / homology | Function and homology information large ribosomal subunit / 5S rRNA binding / transferase activity / ribosomal large subunit assembly / cytosolic large ribosomal subunit / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex ...large ribosomal subunit / 5S rRNA binding / transferase activity / ribosomal large subunit assembly / cytosolic large ribosomal subunit / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / cytoplasm Similarity search - Function | |||||||||
Biological species | Staphylococcus aureus (bacteria) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.48 Å | |||||||||
Authors | Cimicata G / Bashan A / Yonath A | |||||||||
Funding support | 1 items
| |||||||||
Citation | Journal: To Be Published Title: Staphylococcus aureus 50S after 30 minutes incubation a 37C Authors: Cimicata G / Bashan A / Yonath A | |||||||||
History |
|
-Structure visualization
Movie |
Movie viewer |
---|---|
Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_11900.map.gz | 140.6 MB | EMDB map data format | |
---|---|---|---|---|
Header (meta data) | emd-11900-v30.xml emd-11900.xml | 41.1 KB 41.1 KB | Display Display | EMDB header |
Images | emd_11900.png | 91.7 KB | ||
Others | emd_11900_additional_1.map.gz | 166.9 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-11900 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-11900 | HTTPS FTP |
-Validation report
Summary document | emd_11900_validation.pdf.gz | 360.4 KB | Display | EMDB validaton report |
---|---|---|---|---|
Full document | emd_11900_full_validation.pdf.gz | 360 KB | Display | |
Data in XML | emd_11900_validation.xml.gz | 6.9 KB | Display | |
Data in CIF | emd_11900_validation.cif.gz | 7.8 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-11900 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-11900 | HTTPS FTP |
-Related structure data
Related structure data | 7asmMC 7asnC 7asoC 7aspC M: atomic model generated by this map C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
---|---|
Related items in Molecule of the Month |
-Map
File | Download / File: emd_11900.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.858 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-Additional map: #1
File | emd_11900_additional_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Sample components
+Entire : 50S ribosomal subunit
+Supramolecule #1: 50S ribosomal subunit
+Macromolecule #1: 50S ribosomal protein L19
+Macromolecule #2: 50S ribosomal protein L4
+Macromolecule #5: 50S ribosomal protein L2
+Macromolecule #6: 50S ribosomal protein L6
+Macromolecule #7: 50S ribosomal protein L13
+Macromolecule #8: 50S ribosomal protein L15
+Macromolecule #9: 50S ribosomal protein L16
+Macromolecule #10: 50S ribosomal protein L18
+Macromolecule #11: 50S ribosomal protein L20
+Macromolecule #12: 50S ribosomal protein L21
+Macromolecule #13: 50S ribosomal protein L23
+Macromolecule #14: 50S ribosomal protein L24
+Macromolecule #15: 50S ribosomal protein L25
+Macromolecule #16: 50S ribosomal protein L27
+Macromolecule #17: 50S ribosomal protein L29
+Macromolecule #18: 50S ribosomal protein L35
+Macromolecule #19: 50S ribosomal protein L33
+Macromolecule #20: 50S ribosomal protein L34
+Macromolecule #21: 50S ribosomal protein L36
+Macromolecule #22: 50S ribosomal protein L3
+Macromolecule #23: 50S ribosomal protein L5
+Macromolecule #24: 50S ribosomal protein L14
+Macromolecule #25: 50S ribosomal protein L22
+Macromolecule #26: 50S ribosomal protein L28
+Macromolecule #27: 50S ribosomal protein L30
+Macromolecule #28: 50S ribosomal protein L32
+Macromolecule #29: 50S ribosomal protein L17
+Macromolecule #3: 23S rRNA
+Macromolecule #4: 5S rRNA
+Macromolecule #30: MAGNESIUM ION
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.6 |
---|---|
Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 200 |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 47.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.48 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 198422 |
---|---|
Initial angle assignment | Type: NOT APPLICABLE |
Final angle assignment | Type: NOT APPLICABLE |