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- EMDB-11579: Outer Dynein Arm-Shulin complex - Shulin region from Tetrahymena ... -

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Basic information

Entry
Database: EMDB / ID: EMD-11579
TitleOuter Dynein Arm-Shulin complex - Shulin region from Tetrahymena thermophila
Map data
Sample
  • Complex: Shulin region from Outer Dynein Arm-Shulin complex (Tetrahymena thermophila). Contains Shulin (excluding C3 finger), Lc8e, Lc8f, Lc8d, Lc8, Lc2a, Lc9, Dic N-termini and portion of Dyh3 tail.
    • Complex: Dynein
      • Protein or peptide: x 9 types
    • Complex: ShulinShulin District
      • Protein or peptide: x 1 types
  • Ligand: x 1 types
Function / homology
Function and homology information


dynein complex / minus-end-directed microtubule motor activity / dynein light intermediate chain binding / motile cilium / dynein intermediate chain binding / microtubule-based movement / microtubule-based process / microtubule / ATP hydrolysis activity / ATP binding / cytoplasm
Similarity search - Function
Uncharacterized protein C20orf194-like / Dynein light chain Tctex-1 like / Tctex-1-like superfamily / Tctex-1 family / Dynein light chain type 1 / Dynein light chain, type 1/2 / Dynein light chain superfamily / Dynein light chain type 1 / Dynein heavy chain, AAA 5 extension domain / Dynein heavy chain AAA lid domain ...Uncharacterized protein C20orf194-like / Dynein light chain Tctex-1 like / Tctex-1-like superfamily / Tctex-1 family / Dynein light chain type 1 / Dynein light chain, type 1/2 / Dynein light chain superfamily / Dynein light chain type 1 / Dynein heavy chain, AAA 5 extension domain / Dynein heavy chain AAA lid domain / Dynein heavy chain 3, AAA+ lid domain / AAA+ lid domain / Dynein heavy chain, C-terminal domain / Dynein heavy chain, C-terminal domain, barrel region / Dynein heavy chain C-terminal domain / P-loop containing dynein motor region / Dynein heavy chain, tail / Dynein heavy chain, N-terminal region 1 / Dynein heavy chain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, linker / Dynein heavy chain, AAA module D4 / Dynein heavy chain, coiled coil stalk / Dynein heavy chain, hydrolytic ATP-binding dynein motor region / Dynein heavy chain, ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Dynein heavy chain AAA lid domain superfamily / Dynein heavy chain, domain 2, N-terminal / Dynein heavy chain, linker, subdomain 3 / Dynein heavy chain, AAA1 domain, small subdomain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, N-terminal region 2 / Hydrolytic ATP binding site of dynein motor region / Microtubule-binding stalk of dynein motor / P-loop containing dynein motor region D4 / ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Dynein light chain tctex-type 1 protein / Dynein intermediate chain 2 / Dynein light chain 2A / Dynein heavy chain, outer arm protein / Dynein light chain / Uncharacterized protein / Flagellar outer dynein arm intermediate protein, putative / Dynein light chain / Dynein light chain / Dynein light chain
Similarity search - Component
Biological speciesTetrahymena thermophila CU428 (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.3 Å
AuthorsMali GR / Abid Ali F / Lau CK / Carter AP
Funding support United Kingdom, 2 items
OrganizationGrant numberCountry
Wellcome Trust United Kingdom
Medical Research Council (MRC, United Kingdom) United Kingdom
CitationJournal: Science / Year: 2021
Title: Shulin packages axonemal outer dynein arms for ciliary targeting.
Authors: Girish R Mali / Ferdos Abid Ali / Clinton K Lau / Farida Begum / Jérôme Boulanger / Jonathan D Howe / Zhuo A Chen / Juri Rappsilber / Mark Skehel / Andrew P Carter /
Abstract: The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to ...The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to cilia by an unknown mechanism. Here, we used the ciliate to identify two factors (Q22YU3 and Q22MS1) that bind ODAs in the cytoplasm and are required for ODA delivery to cilia. Q22YU3, which we named Shulin, locked the ODA motor domains into a closed conformation and inhibited motor activity. Cryo-electron microscopy revealed how Shulin stabilized this compact form of ODAs by binding to the dynein tails. Our findings provide a molecular explanation for how newly assembled dyneins are packaged for delivery to the cilia.
History
DepositionAug 3, 2020-
Header (metadata) releaseJan 20, 2021-
Map releaseJan 20, 2021-
UpdateMar 17, 2021-
Current statusMar 17, 2021Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.021
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by radius
  • Surface level: 0.021
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-6zyx
  • Surface level: 0.021
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_11579.png

Downloads & links

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Map

FileDownload / File: emd_11579.map.gz / Format: CCP4 / Size: 347.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.11 Å
Density
Contour LevelBy AUTHOR: 0.021 / Movie #1: 0.021
Minimum - Maximum-0.05202343 - 0.066026956
Average (Standard dev.)1.2899099e-05 (±0.0009420755)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions450450450
Spacing450450450
CellA=B=C: 499.5 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.111.111.11
M x/y/z450450450
origin x/y/z0.0000.0000.000
length x/y/z499.500499.500499.500
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS450450450
D min/max/mean-0.0520.0660.000

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Supplemental data

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Mask #1

Fileemd_11579_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram (log scale)

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Mask #2

Fileemd_11579_msk_2.map
Projections & Slices
AxesZYX

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Histogram

Histogram (log scale)

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Additional map: #1

Fileemd_11579_additional_1.map
Projections & Slices
AxesZYX

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Histogram (log scale)

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Half map: #2

Fileemd_11579_half_map_1.map
Projections & Slices
AxesZYX

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Histogram (log scale)

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Half map: #1

Fileemd_11579_half_map_2.map
Projections & Slices
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Sample components

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Entire : Shulin region from Outer Dynein Arm-Shulin complex (Tetrahymena t...

EntireName: Shulin region from Outer Dynein Arm-Shulin complex (Tetrahymena thermophila). Contains Shulin (excluding C3 finger), Lc8e, Lc8f, Lc8d, Lc8, Lc2a, Lc9, Dic N-termini and portion of Dyh3 tail.
Components
  • Complex: Shulin region from Outer Dynein Arm-Shulin complex (Tetrahymena thermophila). Contains Shulin (excluding C3 finger), Lc8e, Lc8f, Lc8d, Lc8, Lc2a, Lc9, Dic N-termini and portion of Dyh3 tail.
    • Complex: Dynein
      • Protein or peptide: Dynein heavy chain, outer arm protein
      • Protein or peptide: Dynein light chain
      • Protein or peptide: Dynein light chain
      • Protein or peptide: Dynein light chain
      • Protein or peptide: Dynein light chain
      • Protein or peptide: Dynein light chain 2A
      • Protein or peptide: Dynein light chain tctex-type 1 protein
      • Protein or peptide: Dynein intermediate chain 2
      • Protein or peptide: Flagellar outer dynein arm intermediate protein, putative
    • Complex: ShulinShulin District
      • Protein or peptide: ShulinShulin District
  • Ligand: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate

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Supramolecule #1: Shulin region from Outer Dynein Arm-Shulin complex (Tetrahymena t...

SupramoleculeName: Shulin region from Outer Dynein Arm-Shulin complex (Tetrahymena thermophila). Contains Shulin (excluding C3 finger), Lc8e, Lc8f, Lc8d, Lc8, Lc2a, Lc9, Dic N-termini and portion of Dyh3 tail.
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#10

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Supramolecule #2: Dynein

SupramoleculeName: Dynein / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#7, #9-#10
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)

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Supramolecule #3: Shulin

SupramoleculeName: Shulin / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #8
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)

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Macromolecule #1: Dynein heavy chain, outer arm protein

MacromoleculeName: Dynein heavy chain, outer arm protein / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 534.328812 KDa
SequenceString: MLSSKYSKRI AWMKTTICDS LQLKDMIVEE SFQYEKNKNL LEQFLSGEGL NKIFAYYQVQ EQAQNDDIKD TGAQDPVLFF TTGDLEKIQ DKAVWFLRIT NPADDKKKAS QQDGNDNDII FGEITPNTVP MLNALMESVY SRQIDHIITE KIQFWGVAEE E QVLEFQQH ...String:
MLSSKYSKRI AWMKTTICDS LQLKDMIVEE SFQYEKNKNL LEQFLSGEGL NKIFAYYQVQ EQAQNDDIKD TGAQDPVLFF TTGDLEKIQ DKAVWFLRIT NPADDKKKAS QQDGNDNDII FGEITPNTVP MLNALMESVY SRQIDHIITE KIQFWGVAEE E QVLEFQQH SNKFSSEVRE AINLMSPGTE HFKLDYEAIS GLSESEKMQH YEMKFNEWIN LISSQLNDDS EVRKDEKDAG PA TELIYWR SRMQKITNWS EQLKSKDFQI VKASLQRHKN HDNQRPRGDE SLSKLMMEYN RLDLLLTDKL NEAKDNVKYL TTL EKFIEP LYNGTPQQII DTLPALMNAI KMIHTIARFY NTTDKMTGLF IKITNQMIKN CKDRILNKKD NGDNPSLYKM IWEQ DPAEL IEVLGSCIKL YCEYKKCYND TKEKVADMPK GKTFDFSDAQ IFGKFDTFVR RLQKLIEIFS NIQQFNALAK HNLEG MDVL TNKFKKIIDD FKKKGHNLLD TANNKFDRDW VEFNVEISHL DGELQNFIDN NFNRFRNIEY SLKLLHKFQS TIKRDS LKH NLTSRYNAIL HNYATELDTI QRVFQDQKSN PPLVRNMPPE AGKIIWARHL FQKITGPINI FPENVINSTE IRRYYGS YN TLGKQLTIYE MWFYQDWVNK IEQSKAALQA TLIVRHDENK KLYVNFDLEI MQLIREAKCL DRQGIEIPES ARIILLQE D KFKTYYNELL YALKEYERIN SKIKPICKNL LLPHIEDLDL KLRPGMVTLT WTSMNIESYL YYVHQGLKKL EQLIINVND IIENRIENNL KTVSKVVLVH LPQDTKPLSL DSFVQLQEEY INSKTDFLTS KNVEVERAVD DLLQTIMLYP LDPHVDPVLP EETKRIKRY YFWYFYQALL NSTQNSLNAM KYRVCGKKIP GANTLQNLKP FFQVEVQLNG DKVTLNPSLQ EIQKSINRAA T AVLRCSKH LYNWDQQNKD STDKATFYDM IACDKEIVKV ILLLTGSIQG TKNKVNEFLS GFTKFEWLCK ESIQESIKKF SK NGPTLQN YEDQLKKFSQ IEEEIEKIVP TYKIGAMELM THNICTSLST WAKEWKLQYS QDLHKRARQL LDSLTEQTKM LST KLSKPV KDIDSLGYVM ETLEQIRKEQ AEIDMKFNPV QEMYSLLDNY LPGGITDKDE MDARSLLRRN WDILIQQAEI KGKE YQHKQ AIYLKELKQS IKDFTNQVSI FRRDYEKNGP MVEGISPAEA MERLRRFEDE YDVKYQMYKI NARGENLFGL QNQKY PELE KTDAEIKNLN KLYNLYDSVI KNIQQFKEKS WQDVSKDDLA KMEEDAGKYG EQCSRLPKDL KEWQAYRDLK NYIDSL REQ LPLIISLKKP SIMPRHWEKI KEITNTKLNY ENPDQFYIEE IMGAKLLDFR EDIEDITESA DKQLKIRTGL DEINLYW ND MQFQFGIWGK RDVPCMLNGL IVGTILERLE EDQLQLSTFN SQRHVTPFKA EVENLIRTFS DVNDTLDMWV KVQKLWTS L EPVFTGGDIA RQMPLQAKQF QGIDKNWMKI MEKAVETKKV IPCCQNDMLK DFLPDLNRKL EDCQKMLEAY LEGKRKKFP RFYFVSNPTL LKILSQGSEP TSIQEDFEKL FDAITKVTFE SAKDKKNPAL KQITQIQQVI GRNEENISLT GYYVKCEGNI EDWLKKLEQ NMQQTLKDIA SAAAQQVFQV GLKEFVSSQA SQIALLGLQI LWTSKVNEGL ERLSRNERNA MDIKRNEIKE H MNILSSMC LEDLNGAVER TKVETLVTIQ VHQKDISMDL KCKDVNDFEW QKQTRIAWKT DIDECIISIT DWDSPYSYEF LG AKERLCI TPLTDRCYIT LAQAMSMYYG GAPAGPAGTG KTETVKDLGR TLGVFVVVTN CSDQHRYRDM AKIFKGLVQS GLW GCFDEF NRIDLEVLSV VAMQVESITT ARKQHMKKFM FPEEEIEIEL IPTVSYFITM NPGYAGRQEL PENLKVLFRG VSMM VPDRE IIIKVKLASV GYLQIDLLAK KFNVLYRLCE EQLSKQRHYD FGLRNILSVL RTAGNTKRQE IKSDEEMLLM RSLRD MNLS KLVADDIPLF NGLLADIFPK LKEVPKKLYP DVEKKIPEEI NAESYLINTP SFQLKIIQLY ETCLVRHGFM LVGPTG SGK STIMKILTEV LTKLGSPHKI VIMNPKAITA EQMYGVKSEI SDDWIPGVFS TIWAKSNNRA LKHTTWITCD GPVDAIW IE NLNTVLDDNK ILTLANGERI AMTENCKVVF EVENLNNASP ATVSRCGQVY VSPTDLGYEA VIEGWIRNRK ASGRAEES D KLGNILRKYL INMRFIELQS KECKEPMMDT SPVISVINIL NLLTGCLQYF VQTQRTLSEQ EYEKFIVYSM AWAIGGIYE AQDRVRFHEL LLAKNAPIPQ KGKENETVFD YYVSQDYLDW KICSPEEWVP PQSLQFSQLL LPTLDSFRAE MLLNFILTQP KSHTCSNSA LLIGGSGTAK TSSVLLYCNK FDPQKMLFKR TNFSSATSPF MFQSTIEAEC DFKVGKEFAP PGNKMMTIFI D DMSMPFVN KWGDQITLEL VRQLIETGGF YMLDKTQRGN QRKMKNLQYI GAMNHPGGGR NDIPNRLKRQ FFIFNMILPL SI EGIYGPI IKHMFKQKYF SDSTYKVIES LTSATIALWN KVKSTMLPTP AKFHYVFNMR ELSRIFKGIL TCKKDTINDA PKS MKIKPE LFLVGLWRHE AERVLADKLV NNKDKDTVMG YIQEVSLESF SQIENEILEK YSSEKTFLFC DFLRPDVINE DGII EEEAP KIYEAIDSLT ELRKRCNFLL SFYNDRNPSK KMPLVLFDDA LKHLLRISRI IRQPRSSGLL VGVGGSGKQS LTRLA GFIG KNLIQQIIVT KTYSDKDLKE DIKKGFDDAG HLGKQVTFLM TDSEVKKEEF LEYINMVLST GEIPNLLAKD EREVWL GDI SQAYCKEKNL GNIDPPQSEL WTYFVDRVRD NFHIMLCFSP VGQKFRERAR KFPALFNECT IDWFLPWPEE ALVSVAE TF IKNFDKLDTK EETKQELMKH MGNVHLMVNE ICDEYYQKMR RQVYVTPKSF LSYLNSYKTL YIEKYDELDQ QEESFKIG L NKIQEATITI NQMEISLKEE EIQLNEATEK TNQLLANLDK ESKKANQKGE EVAATNKQCE IQAEQISKEK EEAERELEA ALPALRRAQE AVDSIESKDI VELKANKKPL DIIKYIMDAV LVFFKARLIP IQIEERVFNK KEGKAVLFLK ESYDESGIQT LGDMNFMKK LKEFEKDSIN EETIELLEPY LNQSEDWFND TFATKASKAA AGILKWAFAI YEYHQKSKIV KPKRIQVAIA E GRQAIALK ELEKAREDLA QIQAYIKNLK DVYTKQMEEK NELEMKAAKT KKKINTARTL ITSLSGEKDR WGKGAQDISD QK RKLVGNV SLSTAFISYC GPFNAEYRNK LAQQRFVVDM KKRGVPVTPG LELTSFLIDD ATIGEWNLQG LPKDDLSIQN GIM VTNSAR YPLFIDPQGQ GQNWIRNKLS ASIIPERCIT TLSHPKFKDM FLKYCMESGL TLIVENIENE VDPMMDPVLE RQII VKGKT QFVNVAGTEM ELSKEFKLFM TCRLANPSFS PELSAKTTII DFTVTQSGLE QQLLGKVISK EQKALEDSLN QLLAD VNQN QKDLQRLDKN LLERLINSQG NLLDDTELMD VLNNTKTQAK EVAAKLIDAE IKTKEINEKR EQYRPVAIRG SAIYFT MIE VSLVNWMYNS SLEQFLKLFI ESIDLSEKAQ LPSNRVKNII SFLTFHVYRY VNRGLFEKDK ITFILMMAFK ILTTAGT IS SGDVSLFLKS GDALDIKSER QKQISYLEDN QWLNILALSK HTFSGQTLPF FKELPDLISR SENQWRNWID KNDPENFP I PDFAESINQE KEIGSFISLC LVRSLRNDRT LIATQNFISN VLGKEFTDPI SYPIEGIWQE SSNMDPVLFL LSAGADPTS SIDELAKKKK KFPCEKVSMG EGQERVARQV IMKGFVEGGW VILQNCHLGL KFMEEIETLV SPINQIHEDF RLWITCEQHP KFPLGLLQK TLKVTNEPPK GLKAGLYKTF TTIITQEFID KVDHSNWRSL IFTICFLHSI VIERKKFGPL GWCVPYEYNY S DLEASLLY IEKYLTNLMS TPQPNSHNLP ISMNVVRYMI CEVQYGGRIT DDLDRELFIT YGETYLKDGI FGNDYFFYDI MV DGSGQKF KYRIPQNPSA ELIKYQEYIA KVPTVDNPEV FGLHSNADLT FRLKESKEMI NTVMETRPKD SSVGGGKTRE EIV QDKAKD MLKNLPPDYN DVEVRELVSK LGGPNPKTST ERGMTVPLNI FLYQEVTRMQ RVIGLVRKTL QDTILAIDGQ IIMT PEILE AINAIYDAKV PNSWLYDPSG AEISWLLPNL GSWSTSLSDR NKQLNDWLRS GQRPILFWLT GFFNPQGFLT GMKQE VTRN HKKGDGKGGE AWSLDDVVYS TTVKEREKEK DIEQPPAEGV YIKGLYLEGC KWSKNGLDDS DPKKIFADLP ILHVSA INK KKTNEQDRMS NTYLCPVYKY PKRTDKYLIF RVGLPCEGSN NPSHWKLRGV ALLCSTE

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Macromolecule #2: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 10.973408 KDa
SequenceString:
MGDHANEQII DMPENSEMKS MKNDAFSQAK FAVENYKFEN KISSHIKKFF DEKYGPNWHC VVGKHFNAYV SYDSKNFIFF YEGQLAILL YRKG

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Macromolecule #3: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 13.336089 KDa
SequenceString:
MASNQQQKED PKEQQQQYKT FMGARVLWPP ECADDILEGA IRETQDALKK FEIAREGQKI AEHLKKYMDD HFDPYWHVFF GKNFGCQAV HNKNRFIYFY IEKTAFLMYQ TQ

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Macromolecule #4: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 12.516457 KDa
SequenceString:
MSDSDSDEGR VVEEPLPPHI IRFNDMAQHL LKKVIRQADV LIKENPQGLE KDIALNLVKF VKSQPEFKIG DGEWQCIIGK NFGCSLTFD ANVLAFFDLL PSRKSILLFK SG

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Macromolecule #5: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 10.453167 KDa
SequenceString:
MNHEPEVKAT DMEEDMIKRV KEIAINAVKE YKQEKQIAHY IKYEFDKIDG YGWNCIVGRN FGSHIIHQTK KYIFFKINEL CLLLWKA

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Macromolecule #6: Dynein light chain 2A

MacromoleculeName: Dynein light chain 2A / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 15.60812 KDa
SequenceString:
MKGTYLYLNI YKRKREASLI TLNYIKNRFY PSKIQKIIKE LFEDRLKGVE YDPNNANQLS ERLVLELREK IKRGKVPRYK IGVQVVFGE IKGQGLRIAS KCLWDVQNDN YASYTYTSEK VYCTGIVFGC YFE

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Macromolecule #7: Dynein light chain tctex-type 1 protein

MacromoleculeName: Dynein light chain tctex-type 1 protein / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 13.202817 KDa
SequenceString:
MGDTDKEYIS EEVQKAIDDS VKQVFGIKDD SSQVTITYNK DKVNLWTQQI IDYTIRGLNK LGKHFKYCVT AILQQTNHAG ISVQITAYQ DTNTDGSLIQ CYEINDIYAI VSVFAMAV

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Macromolecule #8: Shulin

MacromoleculeName: Shulin / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 139.935781 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MFNFFSSANI NQNIPKYSVN DFVFRLKKIE KIVVKEGLDG FLLINGVDSR ENTEYVKLTN WLFLGNSGLE IEENEYLNQI YSDMIVLIK KGTTHIFIDP EALNSLQTLI YSIPNVDVFC PTEKQYEDKD EMELLKMAFF LRVMKPTKKV GILLGQKDKG K INSIEKWP ...String:
MFNFFSSANI NQNIPKYSVN DFVFRLKKIE KIVVKEGLDG FLLINGVDSR ENTEYVKLTN WLFLGNSGLE IEENEYLNQI YSDMIVLIK KGTTHIFIDP EALNSLQTLI YSIPNVDVFC PTEKQYEDKD EMELLKMAFF LRVMKPTKKV GILLGQKDKG K INSIEKWP LIQSYGLEEL GVGFFSMNHE VVDLTLRLNA VYKNYDKFFV SKLIYVVAKR LTGHFNSAAG QLGDMKMHKR NL ATESQLT EIFRDTYEIE EISKWVQIRG VNAALPKPRV LFGKNTSADC SKEPSVAPLK DLKYSETFHS FHATFETFDL RTC LRAART YFLAKGVKEE RNLITLNDDE GVPQGYELNI DENQQYKDQD FLANLYLSII IGFNEVMQLI TKDYKNMTEE FIQD YIFQK VSKVYAGFQI PESEITLDKI QIILKAYNSF GEEVKIDFKD TISFKLTPYF FMVRIEQKNI KSQILNNTVL GSLVF AESF ILQEGCYLLL TKEIPYFDLW NCQNDYSEKI EKMKKRILWE PLGKQISDEL PKNRIFVQTG RKSNYGFDIP IMQASY YMH ELGLRIETQR LGWFILFFKE MKEIQITQKM NHTWLIFKVD SNITFNSISK DTIALEFTGD ALEQSFFKIK NYFEENQ IK YEYQVDIPAI FQESQIAKKQ ILNQQSQGQK LITMNSIQNE QFFISYIESK QLMILNQMKD LKLSAYKNLY EQMQISQA I TPVENHIGVI LVNGSYCSGK RKFAENLIRF GSDNNLRLHL YKFDLNEMSE LTEKSYLSGL LKFASEKKIQ NTDVIVASV PHFINTKILI DYFSKSEKIS NAFYIRTIAT KININNIYSN FNKNPVNNVF TYGVEGYSQF LLLDTYNNYD ADVNALNKTL SGVLPGAKI YKIMNNILNP ALAKDILTSI TFISEQNNLN RLKYSVQYDL LTSNGPSSVV FIPFKLPILR EKIRDLIYKK I LQNGNQTL VDTIEAEQKI AEFKELNKNS KDPLMIEIIK LKEKIEIQNA QTSDQAIKID YVKGILRYDS KLKEGLEEIT IT PNYFIER TVKGVDAKEF TEELNGVSFK NVKYTGITNS IINDMGFVFA GKNLNKEKLL ELLYKLVKPL NKQKLRQRKD LTE EEIVDI QFRNRGEGLE NGEFYDGQFW RNIQGLILPH HPKKDEFIEE YLKQEEVRIN QINEQLQQEW ETWKQVYDKI HLDK

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Macromolecule #9: Dynein intermediate chain 2

MacromoleculeName: Dynein intermediate chain 2 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 77.888219 KDa
SequenceString: MPPKQTKVVA SRKTVMPISR AGRAQIRRKD SNTQNNMNDQ GMEDEEIDQQ REGMKNQYEQ LTAQELNEDM PSKMLEPKNP QAPKNITVY DYYTRKFKTD ELVDQMIVHF SMDGDYIWKE SNEYKTQEEI RDTKKALIKE AMRKQESEEP GANHDEEAIK Q TLRNKFNY ...String:
MPPKQTKVVA SRKTVMPISR AGRAQIRRKD SNTQNNMNDQ GMEDEEIDQQ REGMKNQYEQ LTAQELNEDM PSKMLEPKNP QAPKNITVY DYYTRKFKTD ELVDQMIVHF SMDGDYIWKE SNEYKTQEEI RDTKKALIKE AMRKQESEEP GANHDEEAIK Q TLRNKFNY NTRECQTINP SIRERGVSTE PPPSDTICGN ITQWEIFDAY YAEIMKDHQI ENKKKKEVDQ DKKQDQSMYS TS FKRCCKI MERMVVQNDQ EDKYHDYRYY WSQGDNLEAG KNEGHLLPIW RFSNEKQRKK NVTSICWNPL YPDLFAVSLG SYD FTKQRM GLICLYSLKN TTHPEYAFNC EAGVMCLDFH PKSAALLAVG LYDGTVLVYD IRNKHKKPIY QSTVRNQKHT DPVW QVKWN PDTSKNYNFY SISSDGRVMN WILMKNKLEP EEVILLRLVG KNEEESTLIG LACGLCFDFN KFEPHIFLVG TEEGK IHKC SRAYSGQYQE TYNGHLLAVY KVKWNNFHPR TFISASADWT VRIWDSKYTS QIICFDLSMM VVDAVWAPYS STVFAC ATM DKVQVYDLNV DKLNKLAEQK IVKQPKLTNL SFNYKDPILL VGDSHGGVTL VKLSPNLCKS GPEIKQTEDK KAMEEFK NV KIEDYEREKM ENLLAVVSKW EREDA

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Macromolecule #10: Flagellar outer dynein arm intermediate protein, putative

MacromoleculeName: Flagellar outer dynein arm intermediate protein, putative
type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 77.178062 KDa
SequenceString: MAEYFTYSKK RKEFNNPINF QDTETRYGGI QNQVVNINQY VQRNPNFIDL DNIAELSEHS VNTERVKTGD RGMSHKEGGW PGNVDPNEA QETGRFKKRI EKDTSFPQAV KDLKEGVEKC IYQNNQIDLL EEYFEGETSE HVVENLSSKT LMLFKDEKEI C KRSVSEIS ...String:
MAEYFTYSKK RKEFNNPINF QDTETRYGGI QNQVVNINQY VQRNPNFIDL DNIAELSEHS VNTERVKTGD RGMSHKEGGW PGNVDPNEA QETGRFKKRI EKDTSFPQAV KDLKEGVEKC IYQNNQIDLL EEYFEGETSE HVVENLSSKT LMLFKDEKEI C KRSVSEIS WHPEGPTKVA VSYAIMRFQQ MPEKMPTQAY VWDLLNPNSP EIKLMSPSAV TNISYNQKIP DQIGGGCYNG LL AVWDGRK GENPIMISPV ENSHYEPVTH FHWLMSKTGS ECVTTSTDGK VMWWDTRKFE AGPVEKLNII EGLGENEEII GGT ALEYNV EAGPSKFLIG TESGSILTAN KKLKKPVEIT TRYGLDQGRH LGPVYSINRS NQNPKYFLSV GDWSCKIWVE DLKT PIIRT KYHGSYLSDG CWSPTRSGAF FLVRRDGWMD VWDYYYRQNE IAFSHKVSDS PLTCIKINQT GGAYHNSGKL CAIGD QDGT VTILELCDSL YTMQPKEKDI INEMFEREYR KEKNLETIKK QQELAKRQVQ KDMGSQKEKW EKKKLEMIET AEASFH ENL AKNPVNEEEF NELDSPSEKR KKTNQNQGRE QEEQSREEQE ASGNFNQQQQ QQQEEEQQQE GEQQHHQNQE HQNGQGH EN GQEEGEENGE EGNQQENEGQ EENEQQQE

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Macromolecule #11: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 11 / Number of copies: 1 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.2 mg/mL
BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 52.0 e/Å2

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Image processing

Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 43338
FSC plot (resolution estimation)

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