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- EMDB-11578: Outer Dynein Arm-Shulin complex - ordered tail region (Tetrahymen... -

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Basic information

Entry
Database: EMDB / ID: EMD-11578
TitleOuter Dynein Arm-Shulin complex - ordered tail region (Tetrahymena thermophila)
Map data
SampleTetrahymena thermophila ODA Dyh4 motor
Biological speciesTetrahymena thermophila (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 5.92 Å
AuthorsMali GR / Abid Ali F / Lau CK / Carter AP
Funding support United Kingdom, 2 items
OrganizationGrant numberCountry
Wellcome Trust United Kingdom
Medical Research Council (MRC, United Kingdom) United Kingdom
CitationJournal: Science / Year: 2021
Title: Shulin packages axonemal outer dynein arms for ciliary targeting.
Authors: Girish R Mali / Ferdos Abid Ali / Clinton K Lau / Farida Begum / Jérôme Boulanger / Jonathan D Howe / Zhuo A Chen / Juri Rappsilber / Mark Skehel / Andrew P Carter /
Abstract: The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to ...The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to cilia by an unknown mechanism. Here, we used the ciliate to identify two factors (Q22YU3 and Q22MS1) that bind ODAs in the cytoplasm and are required for ODA delivery to cilia. Q22YU3, which we named Shulin, locked the ODA motor domains into a closed conformation and inhibited motor activity. Cryo-electron microscopy revealed how Shulin stabilized this compact form of ODAs by binding to the dynein tails. Our findings provide a molecular explanation for how newly assembled dyneins are packaged for delivery to the cilia.
Validation ReportSummary, Full report, XML, About validation report
History
DepositionAug 3, 2020-
Header (metadata) releaseJan 20, 2021-
Map releaseJan 20, 2021-
UpdateMar 17, 2021-
Current statusMar 17, 2021Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.00448
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by radius
  • Surface level: 0.00448
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_11578.map.gz / Format: CCP4 / Size: 347.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.11 Å/pix.
x 450 pix.
= 499.5 Å
1.11 Å/pix.
x 450 pix.
= 499.5 Å
1.11 Å/pix.
x 450 pix.
= 499.5 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.11 Å
Density
Contour LevelBy AUTHOR: 0.00448 / Movie #1: 0.00448
Minimum - Maximum-0.015252047 - 0.02575889
Average (Standard dev.)1.6791011e-05 (±0.0007283911)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions450450450
Spacing450450450
CellA=B=C: 499.5 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.111.111.11
M x/y/z450450450
origin x/y/z0.0000.0000.000
length x/y/z499.500499.500499.500
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS450450450
D min/max/mean-0.0150.0260.000

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Supplemental data

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Segmentation: #1

Fileemd_11578_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Additional map: Mask for ordered ODA-Shulin tail (Z-flipped, non resampled)...

Fileemd_11578_additional_1.map
AnnotationMask for ordered ODA-Shulin tail (Z-flipped, non resampled) to be used with half maps.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Half map: Half map 2 of ordered ODA-Shulin tail (Z-flipped, non-resampled)

Fileemd_11578_half_map_1.map
AnnotationHalf map 2 of ordered ODA-Shulin tail (Z-flipped, non-resampled)
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Half map: Half map 1 of ordered ODA-Shulin tail (Z-flipped, non-resampled)

Fileemd_11578_half_map_2.map
AnnotationHalf map 1 of ordered ODA-Shulin tail (Z-flipped, non-resampled)
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Sample components

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Entire Tetrahymena thermophila ODA Dyh4 motor

EntireName: Tetrahymena thermophila ODA Dyh4 motor / Number of components: 1

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Component #1: protein, Tetrahymena thermophila ODA Dyh4 motor

ProteinName: Tetrahymena thermophila ODA Dyh4 motor / Recombinant expression: No
MassTheoretical: 1.8 MDa
SourceSpecies: Tetrahymena thermophila (eukaryote)

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Experimental details

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Sample preparation

SpecimenSpecimen state: Particle / Method: cryo EM
Sample solutionSpecimen conc.: 0.2 mg/mL / pH: 7.4
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Temperature: 277.15 K / Humidity: 100 %

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Electron microscopy imaging

ImagingMicroscope: FEI TITAN
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 52 e/Å2 / Illumination mode: FLOOD BEAM
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: OTHER

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Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 56667
3D reconstructionResolution: 5.92 Å / Resolution method: FSC 0.143 CUT-OFF
FSC plot (resolution estimation)

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