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- EMDB-11582: Outer Dynein Arm-Shulin complex - Dyh4 motor (Tetrahymena thermophila) -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-11582 | |||||||||
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Title | Outer Dynein Arm-Shulin complex - Dyh4 motor (Tetrahymena thermophila) | |||||||||
![]() | Main map of Dyh4 motor (resampled on a full-length ODA-Shulin structure from a smaller subset of particles using EMDA). -131 angstrom sharpened map. | |||||||||
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Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 5.03 Å | |||||||||
![]() | Mali GR / Abid Ali F / Lau CK / Carter AP | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Shulin packages axonemal outer dynein arms for ciliary targeting. Authors: Girish R Mali / Ferdos Abid Ali / Clinton K Lau / Farida Begum / Jérôme Boulanger / Jonathan D Howe / Zhuo A Chen / Juri Rappsilber / Mark Skehel / Andrew P Carter / ![]() ![]() Abstract: The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to ...The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to cilia by an unknown mechanism. Here, we used the ciliate to identify two factors (Q22YU3 and Q22MS1) that bind ODAs in the cytoplasm and are required for ODA delivery to cilia. Q22YU3, which we named Shulin, locked the ODA motor domains into a closed conformation and inhibited motor activity. Cryo-electron microscopy revealed how Shulin stabilized this compact form of ODAs by binding to the dynein tails. Our findings provide a molecular explanation for how newly assembled dyneins are packaged for delivery to the cilia. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 35.3 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 16.2 KB 16.2 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 9.1 KB | Display | ![]() |
Images | ![]() | 49.4 KB | ||
Masks | ![]() | 43.5 MB | ![]() | |
Others | ![]() ![]() ![]() | 228.8 KB 50 MB 50 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Main map of Dyh4 motor (resampled on a full-length ODA-Shulin structure from a smaller subset of particles using EMDA). -131 angstrom sharpened map. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 2.22991 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Mask #1
File | ![]() | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Additional map: Mask for Dyh4 motor map (Z-flipped, non resampled)...
File | emd_11582_additional_1.map | ||||||||||||
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Annotation | Mask for Dyh4 motor map (Z-flipped, non resampled) to be used with half maps. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map 2 of Dyh4 motor map (Z-flipped, non-resampled)
File | emd_11582_half_map_1.map | ||||||||||||
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Annotation | Half map 2 of Dyh4 motor map (Z-flipped, non-resampled) | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map 1 of Dyh4 motor map (Z-flipped, non-resampled)
File | emd_11582_half_map_2.map | ||||||||||||
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Annotation | Half map 1 of Dyh4 motor map (Z-flipped, non-resampled) | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Tetrahymena thermophila ODA Dyh4 motor
Entire | Name: Tetrahymena thermophila ODA Dyh4 motor |
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Components |
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-Supramolecule #1: Tetrahymena thermophila ODA Dyh4 motor
Supramolecule | Name: Tetrahymena thermophila ODA Dyh4 motor / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 1.8 MDa |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 0.2 mg/mL |
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Buffer | pH: 7.4 |
Grid | Model: Quantifoil R2/2 / Material: GOLD / Support film - Material: GRAPHENE OXIDE |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 52.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
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Image processing
-Atomic model buiding 1
Refinement | Protocol: RIGID BODY FIT |
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